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Protein

(E3-independent) E2 ubiquitin-conjugating enzyme

Gene

UBE2O

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).4 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.PROSITE-ProRule annotation2 Publications

Activity regulationi

inhibited by phenylarsine oxide (PAO).1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1040Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processTransport, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS10987-MONOMER
ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ9C0C9
SIGNORiQ9C0C9
UniPathwayi
UPA00143

Protein family/group databases

MoonDBiQ9C0C9 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
(E3-independent) E2 ubiquitin-conjugating enzyme (EC:2.3.2.242 Publications)
Alternative name(s):
E2/E3 hybrid ubiquitin-protein ligase UBE2O
Ubiquitin carrier protein O
Ubiquitin-conjugating enzyme E2 O
Ubiquitin-conjugating enzyme E2 of 230 kDa
Short name:
Ubiquitin-conjugating enzyme E2-230K
Ubiquitin-protein ligase O
Gene namesi
Name:UBE2O
Synonyms:KIAA1734
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000175931.12
HGNCiHGNC:29554 UBE2O
MIMi617649 gene
neXtProtiNX_Q9C0C9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1040C → S: Loss of function. 2 Publications1

Organism-specific databases

OpenTargetsiENSG00000175931
PharmGKBiPA142670651

Polymorphism and mutation databases

BioMutaiUBE2O
DMDMi209572710

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002806371 – 1292(E3-independent) E2 ubiquitin-conjugating enzymeAdd BLAST1292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1
Modified residuei87PhosphoserineCombined sources1
Modified residuei89PhosphoserineCombined sources1
Modified residuei399PhosphoserineCombined sources1
Modified residuei401PhosphoserineCombined sources1
Modified residuei441PhosphoserineCombined sources1
Modified residuei488PhosphothreonineCombined sources1
Modified residuei491PhosphothreonineCombined sources1
Modified residuei515PhosphoserineCombined sources1
Modified residuei836PhosphoserineCombined sources1
Modified residuei838PhosphothreonineBy similarity1
Modified residuei839PhosphoserineBy similarity1
Modified residuei896PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. Phosphorylation affects subcellular location.1 Publication
Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9C0C9
MaxQBiQ9C0C9
PaxDbiQ9C0C9
PeptideAtlasiQ9C0C9
PRIDEiQ9C0C9
ProteomicsDBi80004

PTM databases

iPTMnetiQ9C0C9
PhosphoSitePlusiQ9C0C9

Expressioni

Tissue specificityi

Predominantly expressed in skeletal muscle and heart.1 Publication

Gene expression databases

BgeeiENSG00000175931 Expressed in 185 organ(s), highest expression level in cerebellum
CleanExiHS_UBE2O
ExpressionAtlasiQ9C0C9 baseline and differential
GenevisibleiQ9C0C9 HS

Organism-specific databases

HPAiHPA023605

Interactioni

Subunit structurei

Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain).By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi121973, 96 interactors
IntActiQ9C0C9, 41 interactors
MINTiQ9C0C9
STRINGi9606.ENSP00000323687

Structurei

3D structure databases

ProteinModelPortaliQ9C0C9
SMRiQ9C0C9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili812 – 882Sequence analysisAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi512 – 536Nuclear localization signal1 PublicationAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 43Ala-richAdd BLAST42

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0895 Eukaryota
ENOG410XQ7W LUCA
GeneTreeiENSGT00730000110680
HOVERGENiHBG080116
InParanoidiQ9C0C9
KOiK10581
OMAiKAEWPSE
OrthoDBiEOG091G04SX
PhylomeDBiQ9C0C9
TreeFamiTF325556

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9C0C9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADPAAPTPA APAPAQAPAP APEAVPAPAA APVPAPAPAS DSASGPSSDS
60 70 80 90 100
GPEAGSQRLL FSHDLVSGRY RGSVHFGLVR LIHGEDSDSE GEEEGRGSSG
110 120 130 140 150
CSEAGGAGHE EGRASPLRRG YVRVQWYPEG VKQHVKETKL KLEDRSVVPR
160 170 180 190 200
DVVRHMRSTD SQCGTVIDVN IDCAVKLIGT NCIIYPVNSK DLQHIWPFMY
210 220 230 240 250
GDYIAYDCWL GKVYDLKNQI ILKLSNGARC SMNTEDGAKL YDVCPHVSDS
260 270 280 290 300
GLFFDDSYGF YPGQVLIGPA KIFSSVQWLS GVKPVLSTKS KFRVVVEEVQ
310 320 330 340 350
VVELKVTWIT KSFCPGGTDS VSPPPSVITQ ENLGRVKRLG CFDHAQRQLG
360 370 380 390 400
ERCLYVFPAK VEPAKIAWEC PEKNCAQGEG SMAKKVKRLL KKQVVRIMSC
410 420 430 440 450
SPDTQCSRDH SMEDPDKKGE SKTKSEAESA SPEETPDGSA SPVEMQDEGA
460 470 480 490 500
EEPHEAGEQL PPFLLKEGRD DRLHSAEQDA DDEAADDTDD TSSVTSSASS
510 520 530 540 550
TTSSQSGSGT SRKKSIPLSI KNLKRKHKRK KNKITRDFKP GDRVAVEVVT
560 570 580 590 600
TMTSADVMWQ DGSVECNIRS NDLFPVHHLD NNEFCPGDFV VDKRVQSCPD
610 620 630 640 650
PAVYGVVQSG DHIGRTCMVK WFKLRPSGDD VELIGEEEDV SVYDIADHPD
660 670 680 690 700
FRFRTTDIVI RIGNTEDGAP HKEDEPSVGQ VARVDVSSKV EVVWADNSKT
710 720 730 740 750
IILPQHLYNI ESEIEESDYD SVEGSTSGAS SDEWEDDSDS WETDNGLVED
760 770 780 790 800
EHPKIEEPPI PPLEQPVAPE DKGVVISEEA ATAAVQGAVA MAAPMAGLME
810 820 830 840 850
KAGKDGPPKS FRELKEAIKI LESLKNMTVE QLLTGSPTSP TVEPEKPTRE
860 870 880 890 900
KKFLDDIKKL QENLKKTLDN VAIVEEEKME AVPDVERKED KPEGQSPVKA
910 920 930 940 950
EWPSETPVLC QQCGGKPGVT FTSAKGEVFS VLEFAPSNHS FKKIEFQPPE
960 970 980 990 1000
AKKFFSTVRK EMALLATSLP EGIMVKTFED RMDLFSALIK GPTRTPYEDG
1010 1020 1030 1040 1050
LYLFDIQLPN IYPAVPPHFC YLSQCSGRLN PNLYDNGKVC VSLLGTWIGK
1060 1070 1080 1090 1100
GTERWTSKSS LLQVLISIQG LILVNEPYYN EAGFDSDRGL QEGYENSRCY
1110 1120 1130 1140 1150
NEMALIRVVQ SMTQLVRRPP EVFEQEIRQH FSTGGWRLVN RIESWLETHA
1160 1170 1180 1190 1200
LLEKAQALPN GVPKASSSPE PPAVAELSDS GQQEPEDGGP APGEASQGSD
1210 1220 1230 1240 1250
SEGGAQGLAS ASRDHTDQTS ETAPDASVPP SVKPKKRRKS YRSFLPEKSG
1260 1270 1280 1290
YPDIGFPLFP LSKGFIKSIR GVLTQFRAAL LEAGMPECTE DK
Length:1,292
Mass (Da):141,293
Last modified:October 14, 2008 - v3
Checksum:iC9DF6395A32E9AB0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7ES11K7ES11_HUMAN
(E3-independent) E2 ubiquitin-conju...
UBE2O
807Annotation score:
K7EQ12K7EQ12_HUMAN
(E3-independent) E2 ubiquitin-conju...
UBE2O
189Annotation score:

Sequence cautioni

The sequence AAH22237 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH25977 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH36820 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH51868 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14320 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14948 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB15313 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB21825 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50S → F in BAB21825 (PubMed:11214970).Curated1
Sequence conflicti675E → EQ in BAB15313 (PubMed:14702039).Curated1
Sequence conflicti1047W → C in AAH22237 (PubMed:15489334).Curated1
Sequence conflicti1143E → D in AAH25977 (PubMed:15489334).Curated1
Sequence conflicti1150A → T in BAB14320 (PubMed:14702039).Curated1
Sequence conflicti1261L → P in BAB14320 (PubMed:14702039).Curated1
Sequence conflicti1290E → G in BAB14948 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0311841207G → S4 PublicationsCorresponds to variant dbSNP:rs3803739Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051521 mRNA Translation: BAB21825.1 Different initiation.
AC090699 Genomic DNA No translation available.
CH471099 Genomic DNA Translation: EAW89396.1
BC004525 mRNA Translation: AAH04525.2
BC022237 mRNA Translation: AAH22237.1 Different initiation.
BC025977 mRNA Translation: AAH25977.1 Different initiation.
BC036820 mRNA Translation: AAH36820.1 Different initiation.
BC051868 mRNA Translation: AAH51868.2 Different initiation.
AL832432 mRNA Translation: CAH10644.1
AK022940 mRNA Translation: BAB14320.1 Different initiation.
AK024657 mRNA Translation: BAB14948.1 Different initiation.
AK025999 mRNA Translation: BAB15313.1 Different initiation.
CCDSiCCDS32742.1
RefSeqiNP_071349.3, NM_022066.3
UniGeneiHs.16130

Genome annotation databases

EnsembliENST00000319380; ENSP00000323687; ENSG00000175931
GeneIDi63893
KEGGihsa:63893
UCSCiuc002jrm.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051521 mRNA Translation: BAB21825.1 Different initiation.
AC090699 Genomic DNA No translation available.
CH471099 Genomic DNA Translation: EAW89396.1
BC004525 mRNA Translation: AAH04525.2
BC022237 mRNA Translation: AAH22237.1 Different initiation.
BC025977 mRNA Translation: AAH25977.1 Different initiation.
BC036820 mRNA Translation: AAH36820.1 Different initiation.
BC051868 mRNA Translation: AAH51868.2 Different initiation.
AL832432 mRNA Translation: CAH10644.1
AK022940 mRNA Translation: BAB14320.1 Different initiation.
AK024657 mRNA Translation: BAB14948.1 Different initiation.
AK025999 mRNA Translation: BAB15313.1 Different initiation.
CCDSiCCDS32742.1
RefSeqiNP_071349.3, NM_022066.3
UniGeneiHs.16130

3D structure databases

ProteinModelPortaliQ9C0C9
SMRiQ9C0C9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121973, 96 interactors
IntActiQ9C0C9, 41 interactors
MINTiQ9C0C9
STRINGi9606.ENSP00000323687

Protein family/group databases

MoonDBiQ9C0C9 Predicted

PTM databases

iPTMnetiQ9C0C9
PhosphoSitePlusiQ9C0C9

Polymorphism and mutation databases

BioMutaiUBE2O
DMDMi209572710

Proteomic databases

EPDiQ9C0C9
MaxQBiQ9C0C9
PaxDbiQ9C0C9
PeptideAtlasiQ9C0C9
PRIDEiQ9C0C9
ProteomicsDBi80004

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319380; ENSP00000323687; ENSG00000175931
GeneIDi63893
KEGGihsa:63893
UCSCiuc002jrm.5 human

Organism-specific databases

CTDi63893
EuPathDBiHostDB:ENSG00000175931.12
GeneCardsiUBE2O
H-InvDBiHIX0020035
HGNCiHGNC:29554 UBE2O
HPAiHPA023605
MIMi617649 gene
neXtProtiNX_Q9C0C9
OpenTargetsiENSG00000175931
PharmGKBiPA142670651
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0895 Eukaryota
ENOG410XQ7W LUCA
GeneTreeiENSGT00730000110680
HOVERGENiHBG080116
InParanoidiQ9C0C9
KOiK10581
OMAiKAEWPSE
OrthoDBiEOG091G04SX
PhylomeDBiQ9C0C9
TreeFamiTF325556

Enzyme and pathway databases

UniPathwayi
UPA00143

BioCyciMetaCyc:HS10987-MONOMER
ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ9C0C9
SIGNORiQ9C0C9

Miscellaneous databases

ChiTaRSiUBE2O human
GeneWikiiUBE2O
GenomeRNAii63893
PROiPR:Q9C0C9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175931 Expressed in 185 organ(s), highest expression level in cerebellum
CleanExiHS_UBE2O
ExpressionAtlasiQ9C0C9 baseline and differential
GenevisibleiQ9C0C9 HS

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2O_HUMAN
AccessioniPrimary (citable) accession number: Q9C0C9
Secondary accession number(s): A6NDU5
, Q69YP4, Q6PIZ2, Q86UA4, Q8N425, Q8TBN1, Q9BSW1, Q9H6E6, Q9H7E4, Q9H9B2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 14, 2008
Last modified: November 7, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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