UniProtKB - Q9C0C9 (UBE2O_HUMAN)
Protein
(E3-independent) E2 ubiquitin-conjugating enzyme
Gene
UBE2O
Organism
Homo sapiens (Human)
Status
Functioni
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).4 Publications
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.PROSITE-ProRule annotation2 Publications EC:2.3.2.24
Activity regulationi
inhibited by phenylarsine oxide (PAO).1 Publication
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1040 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type endopeptidase inhibitor activity Source: GO_Central
- RNA binding Source: UniProtKB
- ubiquitin conjugating enzyme activity Source: MGI
- ubiquitin protein ligase activity Source: MGI
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- negative regulation of apoptotic process Source: GO_Central
- positive regulation of BMP signaling pathway Source: UniProtKB
- protein K63-linked ubiquitination Source: UniProtKB
- protein monoubiquitination Source: UniProtKB
- retrograde transport, endosome to Golgi Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Transport, Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS10987-MONOMER |
PathwayCommonsi | Q9C0C9 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q9C0C9 |
SIGNORi | Q9C0C9 |
UniPathwayi | UPA00143 |
Protein family/group databases
MoonDBi | Q9C0C9, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: (E3-independent) E2 ubiquitin-conjugating enzyme (EC:2.3.2.242 Publications)Alternative name(s): E2/E3 hybrid ubiquitin-protein ligase UBE2O Ubiquitin carrier protein O Ubiquitin-conjugating enzyme E2 O Ubiquitin-conjugating enzyme E2 of 230 kDa Short name: Ubiquitin-conjugating enzyme E2-230K Ubiquitin-protein ligase O |
Gene namesi | Name:UBE2O Synonyms:KIAA1734 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29554, UBE2O |
MIMi | 617649, gene |
neXtProti | NX_Q9C0C9 |
VEuPathDBi | HostDB:ENSG00000175931.12 |
Subcellular locationi
Cytosol
- cytosol Source: GOC
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1040 | C → S: Loss of function. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 63893 |
OpenTargetsi | ENSG00000175931 |
PharmGKBi | PA142670651 |
Miscellaneous databases
Pharosi | Q9C0C9, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295940 |
Genetic variation databases
BioMutai | UBE2O |
DMDMi | 209572710 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000280637 | 1 – 1292 | (E3-independent) E2 ubiquitin-conjugating enzymeAdd BLAST | 1292 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 50 | PhosphoserineCombined sources | 1 | |
Modified residuei | 87 | PhosphoserineCombined sources | 1 | |
Modified residuei | 89 | PhosphoserineCombined sources | 1 | |
Modified residuei | 399 | PhosphoserineCombined sources | 1 | |
Modified residuei | 401 | PhosphoserineCombined sources | 1 | |
Modified residuei | 441 | PhosphoserineCombined sources | 1 | |
Modified residuei | 488 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 491 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 515 | PhosphoserineCombined sources | 1 | |
Modified residuei | 836 | PhosphoserineCombined sources | 1 | |
Modified residuei | 838 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 839 | PhosphoserineBy similarity | 1 | |
Modified residuei | 896 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated. Phosphorylation affects subcellular location.1 Publication
Ubiquitinated: autoubiquitinates, possibly affecting its subcellular location.1 Publication
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9C0C9 |
jPOSTi | Q9C0C9 |
MassIVEi | Q9C0C9 |
MaxQBi | Q9C0C9 |
PaxDbi | Q9C0C9 |
PeptideAtlasi | Q9C0C9 |
PRIDEi | Q9C0C9 |
ProteomicsDBi | 80004 |
PTM databases
iPTMneti | Q9C0C9 |
MetOSitei | Q9C0C9 |
PhosphoSitePlusi | Q9C0C9 |
SwissPalmi | Q9C0C9 |
Expressioni
Tissue specificityi
Predominantly expressed in skeletal muscle and heart.1 Publication
Gene expression databases
Bgeei | ENSG00000175931, Expressed in cerebellum and 199 other tissues |
ExpressionAtlasi | Q9C0C9, baseline and differential |
Genevisiblei | Q9C0C9, HS |
Organism-specific databases
HPAi | ENSG00000175931, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain).
By similarityBinary interactionsi
Hide detailsQ9C0C9
Protein-protein interaction databases
BioGRIDi | 121973, 127 interactors |
IntActi | Q9C0C9, 91 interactors |
MINTi | Q9C0C9 |
STRINGi | 9606.ENSP00000323687 |
Miscellaneous databases
RNActi | Q9C0C9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 953 – 1113 | UBC corePROSITE-ProRule annotationAdd BLAST | 161 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 812 – 882 | Sequence analysisAdd BLAST | 71 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 512 – 536 | Nuclear localization signal1 PublicationAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 2 – 43 | Ala-richAdd BLAST | 42 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0895, Eukaryota |
GeneTreei | ENSGT00940000160755 |
HOGENOMi | CLU_002088_1_0_1 |
InParanoidi | Q9C0C9 |
OMAi | YESYMLQ |
OrthoDBi | 808738at2759 |
PhylomeDBi | Q9C0C9 |
TreeFami | TF325556 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS50127, UBC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9C0C9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADPAAPTPA APAPAQAPAP APEAVPAPAA APVPAPAPAS DSASGPSSDS
60 70 80 90 100
GPEAGSQRLL FSHDLVSGRY RGSVHFGLVR LIHGEDSDSE GEEEGRGSSG
110 120 130 140 150
CSEAGGAGHE EGRASPLRRG YVRVQWYPEG VKQHVKETKL KLEDRSVVPR
160 170 180 190 200
DVVRHMRSTD SQCGTVIDVN IDCAVKLIGT NCIIYPVNSK DLQHIWPFMY
210 220 230 240 250
GDYIAYDCWL GKVYDLKNQI ILKLSNGARC SMNTEDGAKL YDVCPHVSDS
260 270 280 290 300
GLFFDDSYGF YPGQVLIGPA KIFSSVQWLS GVKPVLSTKS KFRVVVEEVQ
310 320 330 340 350
VVELKVTWIT KSFCPGGTDS VSPPPSVITQ ENLGRVKRLG CFDHAQRQLG
360 370 380 390 400
ERCLYVFPAK VEPAKIAWEC PEKNCAQGEG SMAKKVKRLL KKQVVRIMSC
410 420 430 440 450
SPDTQCSRDH SMEDPDKKGE SKTKSEAESA SPEETPDGSA SPVEMQDEGA
460 470 480 490 500
EEPHEAGEQL PPFLLKEGRD DRLHSAEQDA DDEAADDTDD TSSVTSSASS
510 520 530 540 550
TTSSQSGSGT SRKKSIPLSI KNLKRKHKRK KNKITRDFKP GDRVAVEVVT
560 570 580 590 600
TMTSADVMWQ DGSVECNIRS NDLFPVHHLD NNEFCPGDFV VDKRVQSCPD
610 620 630 640 650
PAVYGVVQSG DHIGRTCMVK WFKLRPSGDD VELIGEEEDV SVYDIADHPD
660 670 680 690 700
FRFRTTDIVI RIGNTEDGAP HKEDEPSVGQ VARVDVSSKV EVVWADNSKT
710 720 730 740 750
IILPQHLYNI ESEIEESDYD SVEGSTSGAS SDEWEDDSDS WETDNGLVED
760 770 780 790 800
EHPKIEEPPI PPLEQPVAPE DKGVVISEEA ATAAVQGAVA MAAPMAGLME
810 820 830 840 850
KAGKDGPPKS FRELKEAIKI LESLKNMTVE QLLTGSPTSP TVEPEKPTRE
860 870 880 890 900
KKFLDDIKKL QENLKKTLDN VAIVEEEKME AVPDVERKED KPEGQSPVKA
910 920 930 940 950
EWPSETPVLC QQCGGKPGVT FTSAKGEVFS VLEFAPSNHS FKKIEFQPPE
960 970 980 990 1000
AKKFFSTVRK EMALLATSLP EGIMVKTFED RMDLFSALIK GPTRTPYEDG
1010 1020 1030 1040 1050
LYLFDIQLPN IYPAVPPHFC YLSQCSGRLN PNLYDNGKVC VSLLGTWIGK
1060 1070 1080 1090 1100
GTERWTSKSS LLQVLISIQG LILVNEPYYN EAGFDSDRGL QEGYENSRCY
1110 1120 1130 1140 1150
NEMALIRVVQ SMTQLVRRPP EVFEQEIRQH FSTGGWRLVN RIESWLETHA
1160 1170 1180 1190 1200
LLEKAQALPN GVPKASSSPE PPAVAELSDS GQQEPEDGGP APGEASQGSD
1210 1220 1230 1240 1250
SEGGAQGLAS ASRDHTDQTS ETAPDASVPP SVKPKKRRKS YRSFLPEKSG
1260 1270 1280 1290
YPDIGFPLFP LSKGFIKSIR GVLTQFRAAL LEAGMPECTE DK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7ES11 | K7ES11_HUMAN | (E3-independent) E2 ubiquitin-conju... | UBE2O | 807 | Annotation score: | ||
K7EQ12 | K7EQ12_HUMAN | (E3-independent) E2 ubiquitin-conju... | UBE2O | 189 | Annotation score: |
Sequence cautioni
The sequence AAH22237 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAH25977 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAH36820 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAH51868 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB14320 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB14948 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB15313 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAB21825 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 50 | S → F in BAB21825 (PubMed:11214970).Curated | 1 | |
Sequence conflicti | 675 | E → EQ in BAB15313 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 1047 | W → C in AAH22237 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1143 | E → D in AAH25977 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1150 | A → T in BAB14320 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 1261 | L → P in BAB14320 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 1290 | E → G in BAB14948 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031184 | 1207 | G → S4 PublicationsCorresponds to variant dbSNP:rs3803739Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB051521 mRNA Translation: BAB21825.1 Different initiation. AC090699 Genomic DNA No translation available. CH471099 Genomic DNA Translation: EAW89396.1 BC004525 mRNA Translation: AAH04525.2 BC022237 mRNA Translation: AAH22237.1 Different initiation. BC025977 mRNA Translation: AAH25977.1 Different initiation. BC036820 mRNA Translation: AAH36820.1 Different initiation. BC051868 mRNA Translation: AAH51868.2 Different initiation. AL832432 mRNA Translation: CAH10644.1 AK022940 mRNA Translation: BAB14320.1 Different initiation. AK024657 mRNA Translation: BAB14948.1 Different initiation. AK025999 mRNA Translation: BAB15313.1 Different initiation. |
CCDSi | CCDS32742.1 |
RefSeqi | NP_071349.3, NM_022066.3 |
Genome annotation databases
Ensembli | ENST00000319380; ENSP00000323687; ENSG00000175931 |
GeneIDi | 63893 |
KEGGi | hsa:63893 |
UCSCi | uc002jrm.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB051521 mRNA Translation: BAB21825.1 Different initiation. AC090699 Genomic DNA No translation available. CH471099 Genomic DNA Translation: EAW89396.1 BC004525 mRNA Translation: AAH04525.2 BC022237 mRNA Translation: AAH22237.1 Different initiation. BC025977 mRNA Translation: AAH25977.1 Different initiation. BC036820 mRNA Translation: AAH36820.1 Different initiation. BC051868 mRNA Translation: AAH51868.2 Different initiation. AL832432 mRNA Translation: CAH10644.1 AK022940 mRNA Translation: BAB14320.1 Different initiation. AK024657 mRNA Translation: BAB14948.1 Different initiation. AK025999 mRNA Translation: BAB15313.1 Different initiation. |
CCDSi | CCDS32742.1 |
RefSeqi | NP_071349.3, NM_022066.3 |
3D structure databases
SMRi | Q9C0C9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 121973, 127 interactors |
IntActi | Q9C0C9, 91 interactors |
MINTi | Q9C0C9 |
STRINGi | 9606.ENSP00000323687 |
Chemistry databases
ChEMBLi | CHEMBL4295940 |
Protein family/group databases
MoonDBi | Q9C0C9, Predicted |
PTM databases
iPTMneti | Q9C0C9 |
MetOSitei | Q9C0C9 |
PhosphoSitePlusi | Q9C0C9 |
SwissPalmi | Q9C0C9 |
Genetic variation databases
BioMutai | UBE2O |
DMDMi | 209572710 |
Proteomic databases
EPDi | Q9C0C9 |
jPOSTi | Q9C0C9 |
MassIVEi | Q9C0C9 |
MaxQBi | Q9C0C9 |
PaxDbi | Q9C0C9 |
PeptideAtlasi | Q9C0C9 |
PRIDEi | Q9C0C9 |
ProteomicsDBi | 80004 |
Protocols and materials databases
Antibodypediai | 32408, 155 antibodies |
Genome annotation databases
Ensembli | ENST00000319380; ENSP00000323687; ENSG00000175931 |
GeneIDi | 63893 |
KEGGi | hsa:63893 |
UCSCi | uc002jrm.5, human |
Organism-specific databases
CTDi | 63893 |
DisGeNETi | 63893 |
GeneCardsi | UBE2O |
HGNCi | HGNC:29554, UBE2O |
HPAi | ENSG00000175931, Low tissue specificity |
MIMi | 617649, gene |
neXtProti | NX_Q9C0C9 |
OpenTargetsi | ENSG00000175931 |
PharmGKBi | PA142670651 |
VEuPathDBi | HostDB:ENSG00000175931.12 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0895, Eukaryota |
GeneTreei | ENSGT00940000160755 |
HOGENOMi | CLU_002088_1_0_1 |
InParanoidi | Q9C0C9 |
OMAi | YESYMLQ |
OrthoDBi | 808738at2759 |
PhylomeDBi | Q9C0C9 |
TreeFami | TF325556 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BioCyci | MetaCyc:HS10987-MONOMER |
PathwayCommonsi | Q9C0C9 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q9C0C9 |
SIGNORi | Q9C0C9 |
Miscellaneous databases
BioGRID-ORCSi | 63893, 53 hits in 873 CRISPR screens |
ChiTaRSi | UBE2O, human |
GeneWikii | UBE2O |
GenomeRNAii | 63893 |
Pharosi | Q9C0C9, Tbio |
PROi | PR:Q9C0C9 |
RNActi | Q9C0C9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000175931, Expressed in cerebellum and 199 other tissues |
ExpressionAtlasi | Q9C0C9, baseline and differential |
Genevisiblei | Q9C0C9, HS |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE2O_HUMAN | |
Accessioni | Q9C0C9Primary (citable) accession number: Q9C0C9 Secondary accession number(s): A6NDU5 Q9H9B2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | October 14, 2008 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families