AMBRA1

Functionⁱ

Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex involved in cell cycle control and autophagy (PubMed:20921139, PubMed:23524951, PubMed:24587252, PubMed:33854232, PubMed:33854235, PubMed:33854239, PubMed:32333458).

The DCX(AMBRA1) complex specifically mediates the polyubiquitination of target proteins such as BECN1, CCND1, CCND2, CCND3, ELOC and ULK1 (PubMed:23524951, PubMed:33854232, PubMed:33854235, PubMed:33854239).

Acts as an upstream master regulator of the transition from G1 to S cell phase: AMBRA1 specifically recognizes and binds phosphorylated cyclin-D (CCND1, CCND2 and CCND3), leading to cyclin-D ubiquitination by the DCX(AMBRA1) complex and subsequent degradation (PubMed:33854232, PubMed:33854235, PubMed:33854239).

By controlling the transition from G1 to S phase and cyclin-D degradation, AMBRA1 acts as a tumor suppressor that promotes genomic integrity during DNA replication and counteracts developmental abnormalities and tumor growth (PubMed:33854232, PubMed:33854235, PubMed:33854239).

AMBRA1 also regulates the cell cycle by promoting MYC dephosphorylation and degradation independently of the DCX(AMBRA1) complex: acts via interaction with the catalytic subunit of protein phosphatase 2A (PPP2CA), which enhances interaction between PPP2CA and MYC, leading to MYC dephosphorylation and degradation (PubMed:25803737, PubMed:25438055).

Acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes (PubMed:25499913, PubMed:30166453).

Acts as a key regulator of autophagy by modulating the BECN1-PIK3C3 complex: controls protein turnover during neuronal development, and regulates normal cell survival and proliferation (PubMed:21358617).

In normal conditions, AMBRA1 is tethered to the cytoskeleton via interaction with dyneins DYNLL1 and DYNLL2 (PubMed:20921139).

Upon autophagy induction, AMBRA1 is released from the cytoskeletal docking site to induce autophagosome nucleation by mediating ubiquitination of proteins involved in autophagy (PubMed:20921139).

The DCX(AMBRA1) complex mediates 'Lys-63'-linked ubiquitination of BECN1, increasing the association between BECN1 and PIK3C3 to promote PIK3C3 activity (By similarity).

In collaboration with TRAF6, AMBRA1 mediates 'Lys-63'-linked ubiquitination of ULK1 following autophagy induction, promoting ULK1 stability and kinase activity (PubMed:23524951).

Also activates ULK1 via interaction with TRIM32: TRIM32 stimulates ULK1 through unanchored 'Lys-63'-linked polyubiquitin chains (PubMed:31123703).

Also acts as an activator of mitophagy via interaction with PRKN and LC3 proteins (MAP1LC3A, MAP1LC3B or MAP1LC3C); possibly by bringing damaged mitochondria onto autophagosomes (PubMed:21753002, PubMed:25215947).

Also activates mitophagy by acting as a cofactor for HUWE1; acts by promoting HUWE1-mediated ubiquitination of MFN2 (PubMed:30217973).

AMBRA1 is also involved in regulatory T-cells (Treg) differentiation by promoting FOXO3 dephosphorylation independently of the DCX(AMBRA1) complex: acts via interaction with PPP2CA, which enhances interaction between PPP2CA and FOXO3, leading to FOXO3 dephosphorylation and stabilization (PubMed:30513302).

May act as a regulator of intracellular trafficking, regulating the localization of active PTK2/FAK and SRC (By similarity).

Also involved in transcription regulation by acting as a scaffold for protein complexes at chromatin (By similarity).

By similarity17 Publications

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.5 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein
Biological process	Autophagy, Cell cycle, Differentiation, Neurogenesis, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

PathwayCommonsⁱ	Q9C0C7
Reactomeⁱ	R-HSA-1632852, Macroautophagy
SignaLinkⁱ	Q9C0C7
SIGNORⁱ	Q9C0C7
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Activating molecule in BECN1-regulated autophagy protein 11 Publication Alternative name(s): DDB1- and CUL4-associated factor 31 Publication
Gene namesⁱ	Name:AMBRA11 PublicationImported Synonyms:DCAF31 Publication, KIAA17361 Publication
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 11

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:25990, AMBRA1
MIMⁱ	611359, gene
neXtProtⁱ	NX_Q9C0C7
VEuPathDBⁱ	HostDB:ENSG00000110497

Keywords - Cellular componentⁱ

Cell junction, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Mitochondrion, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	1 – 43	Missing : Abolished interaction with DDB1. 1 PublicationAdd BLAST	43
Mutagenesisⁱ	1 – 22	Missing : Abolished interaction with DDB1. 1 PublicationAdd BLAST	22
Mutagenesisⁱ	52	S → A: Impaired phosphorylation by MTOR, leading to strong induction of autophagy. Does not affect interaction with DDB1. 1 Publication	1
Mutagenesisⁱ	52	S → E: Phospho-mimetic mutant; abolished ability to promote autophagy. Does not affect interaction with DDB1. 1 Publication	1
Mutagenesisⁱ	275 – 277	PQP → AQA: Abolished interaction with PPP2CA and MYC, leading to decreased MYC dephosphorylation; when associated with 1206-A--A-1208. Abolished interaction with PPP2CA and FOXO3, leading to decreased FOXO3 dephosphorylation; when associated with 1206-A--A-1208. 2 Publications	3
Mutagenesisⁱ	482	D → A: Abolished cleavage by caspases without affecting cleavage by calpains. 1 Publication	1
Mutagenesisⁱ	620	E → A: Abolished interaction with TRAF6. 1 Publication	1
Mutagenesisⁱ	642	E → A: Does not affect interaction with TRAF6. 1 Publication	1
Mutagenesisⁱ	682	E → A: Abolished interaction with TRAF6. 1 Publication	1
Mutagenesisⁱ	918	E → A: Does not affect interaction with TRAF6. 1 Publication	1
Mutagenesisⁱ	1043	S → A: Abolished phosphorylation by CHUK/IKKA, leading to impaired interaction with ATG8 family proteins and reduced mitophagic activity. 1 Publication	1
Mutagenesisⁱ	1043	S → D: Phospho-mimetic mutant; increased interaction with ATG8 family proteins and increased mitophagic activity. 1 Publication	1
Mutagenesisⁱ	1049 – 1052	WDQL → ADQA: Abolished interaction with LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). 1 Publication	4
Mutagenesisⁱ	1105	Q → A in TAT1 mutant; abolished interaction with DYNLL1 and DYNLL2, leading to constitutive induction of autophagy. 1 Publication	1
Mutagenesisⁱ	1117	Q → A in TAT2 mutant; abolished interaction with DYNLL1 and DYNLL2, leading to constitutive induction of autophagy. 1 Publication	1
Mutagenesisⁱ	1134	E → A: Does not affect interaction with TRAF6. 1 Publication	1
Mutagenesisⁱ	1206 – 1208	PQP → AQA: Abolished interaction with PPP2CA and MYC, leading to decreased MYC dephosphorylation; when associated with 275-A--A-277. Abolished interaction with PPP2CA and FOXO3, leading to decreased FOXO3 dephosphorylation; when associated with 275-A--A-277. 1 Publication	3

Keywords - Diseaseⁱ

Tumor suppressor

Organism-specific databases

DisGeNET More...DisGeNETⁱ	55626
Open Targets More...OpenTargetsⁱ	ENSG00000110497
PharmGKBⁱ	PA162376307

Miscellaneous databases

Pharosⁱ	Q9C0C7, Tbio

Pharosⁱ

Q9C0C7, Tbio

Genetic variation databases

BioMutaⁱ	AMBRA1
DMDMⁱ	166215833

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000315703	1 – 1298	Activating molecule in BECN1-regulated autophagy protein 1Add BLAST		1298

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	45	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	52	Phosphoserine; by MTOR1 Publication	1
Modified residueⁱ	328	PhosphoserineCombined sources	1
Modified residueⁱ	394	PhosphoserineCombined sources	1
Modified residueⁱ	443	PhosphoserineCombined sources	1
Modified residueⁱ	635	PhosphoserineCombined sources	1
Modified residueⁱ	639	PhosphoserineCombined sources	1
Modified residueⁱ	747	Asymmetric dimethylarginineBy similarity	1
Modified residueⁱ	1043	Phosphoserine; by IKKA1 Publication	1
Modified residueⁱ	1205	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylation at Ser-52 by MTOR inhibits its ability to regulate autophagy and mediate ubiquitination of ULK1 (PubMed:23524951). Phosphorylation by ULK1 in response to autophagy induction abolishes interaction with DYNLL1 and DYNLL2, releasing AMBRA1 from the cytoskeletal docking site to induce autophagosome nucleation (PubMed:20921139). Phosphorylation by MTOR inhibits interaction with PPP2CA and subsequent dephosphorylation of MYC (PubMed:25438055). Phosphorylation at Ser-1043 by CHUK/IKKA promotes its interaction with ATG8 family proteins GABARAP and MAP1LC3B and its mitophagic activity (PubMed:30217973).4 Publications

Ubiquitinated by RNF2 via 'Lys-48'-linkage in unstressed cells, leading to its degradation by the proteasome (PubMed:25499913). Induction of autophagy promotes stabilization via interaction with CUL4 (CUL4A or CUL4B) and DDB1 (PubMed:25499913). Upon prolonged starvation, ubiquitinated and degraded, terminating the autophagy response (PubMed:25499913).1 Publication

Undergoes proteolytic processing by caspase-6 (CASP6), caspase-7 (CASP7) and caspase-8 (CASP8) during apoptosis, resulting in the dismantling of the autophagic machinery and the accomplishment of the programmed cell death program (PubMed:22441670). Also cleaved by calpains during apoptosis, which mediate a complete proteolytic degradation (PubMed:22441670).1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	482 – 483	Cleavage; by caspase-6, caspase-7 and caspase-81 Publication		2

Keywords - PTMⁱ

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q9C0C7
jPOSTⁱ	Q9C0C7
MassIVEⁱ	Q9C0C7
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9C0C7
PaxDbⁱ	Q9C0C7
PeptideAtlas More...PeptideAtlasⁱ	Q9C0C7
PRIDEⁱ	Q9C0C7
ProteomicsDBⁱ	32296 79999 [Q9C0C7-1] 80000 [Q9C0C7-2] 80001 [Q9C0C7-3] 80002 [Q9C0C7-4] 80003 [Q9C0C7-5]

PTM databases

GlyGenⁱ	Q9C0C7, 3 sites, 1 O-linked glycan (3 sites)
iPTMnetⁱ	Q9C0C7
PhosphoSitePlusⁱ	Q9C0C7

Expressionⁱ

Inductionⁱ

Negatively regulated by microRNA 7-3HG (miR7-3HG), which targets the 3' untranslated (3'-UTR) region of AMBRA1 transcripts, leading to a decrease of AMBRA1 mRNA and protein levels, thereby inhibiting autophagy (PubMed:28059583). Strongly up-regulated during egulatory T-cells (Treg) differentiation (PubMed:30513302).2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000110497, Expressed in stomach and 242 other tissues
ExpressionAtlasⁱ	Q9C0C7, baseline and differential
Genevisibleⁱ	Q9C0C7, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000110497, Low tissue specificity

HPAⁱ

ENSG00000110497, Low tissue specificity

Interactionⁱ

Subunit structureⁱ

Component of the DCX(AMBRA1) E3 ubiquitin ligase complex, also named CRL4(AMBRA1), at least composed of CUL4 (CUL4A or CUL4B), DDB1, AMBRA1 and RBX1 (PubMed:25499913, PubMed:16949367, PubMed:30166453, PubMed:33854232, PubMed:33854239).

Interacts with BECN1 (PubMed:21358617, PubMed:25438055). Probably forms a complex with BECN1 and PIK3C3 (By similarity).

Interacts with BECN2 (PubMed:23954414).

Interacts with BCL2; leading to prevent interaction with BCN1 and autophagy, interaction is disrupted upon autophagy induction (PubMed:21358617).

Interacts with ULK1 (PubMed:25438055).

Interacts (via PxP motifs) with PPP2CA; enhancing interaction between PPP2CA and MYC or FOXO3 (PubMed:25438055, PubMed:30513302).

Forms a complex with PPP2CA and BECN1; AMBRA1 and BECN1 components of the complex regulate MYC stability via different pathways (PubMed:25803737).

Interacts (TQT motifs) with DYNLL1 and DYNLL2; tethering AMBRA1 and the BECN1-PIK3C3 complex in absence of autophagy (PubMed:20921139).

Interacts with TRAF6; interaction is required to mediate 'Lys-63'-linked ubiquitination of ULK1 (PubMed:23524951).

Interacts with TRIM32; promoting activation of ULK1 by TRIM32 via unanchored 'Lys-63'-linked polyubiquitin chains (PubMed:31123703).

Interacts with PRKN (By similarity).

Interacts (via LIR motif) with LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C) (PubMed:25215947, PubMed:30217973).

Interacts with HUWE1 (PubMed:30217973).

Interacts with PTK2/FAK (By similarity).

Interacts with SRC; required for SRC trafficking to autophagosomes (By similarity).

By similarity15 Publications

Binary interactionsⁱ

Hide details

Q9C0C7

With	#Exp.	IntAct
BCL2 [P10415]	10	EBI-2512975,EBI-77694
BECN1 [Q14457]	9	EBI-2512975,EBI-949378
PPP2R1A [P30153]	3	EBI-2512975,EBI-302388
ULK1 [O75385]	3	EBI-2512975,EBI-908831
vif [P12504] from Human immunodeficiency virus type 1 group M subtype B (isolate NY5).	5	EBI-2512975,EBI-779991

Isoform 3 [Q9C0C7-3]

With	#Exp.	IntAct
TRAF6 [Q9Y4K3]	2	EBI-16042318,EBI-359276
ULK1 [O75385]	4	EBI-16042318,EBI-908831

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	120765, 233 interactors
Database of interacting proteins More...DIPⁱ	DIP-53597N
IntActⁱ	Q9C0C7, 53 interactors
Molecular INTeraction database More...MINTⁱ	Q9C0C7
STRINGⁱ	9606.ENSP00000431926

Miscellaneous databases

RNActⁱ	Q9C0C7, protein

RNActⁱ

Q9C0C7, protein

Structureⁱ

3D structure databases

SMRⁱ	Q9C0C7
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	51 – 90	WD 1Add BLAST	40
Repeatⁱ	93 – 133	WD 2Add BLAST	41
Repeatⁱ	135 – 175	WD 3Add BLAST	41

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 22	Interaction with DDB11 PublicationAdd BLAST	22
Regionⁱ	254 – 284	DisorderedSequence analysisAdd BLAST	31
Regionⁱ	343 – 413	DisorderedSequence analysisAdd BLAST	71
Regionⁱ	458 – 494	DisorderedSequence analysisAdd BLAST	37
Regionⁱ	538 – 561	DisorderedSequence analysisAdd BLAST	24
Regionⁱ	590 – 690	DisorderedSequence analysisAdd BLAST	101
Regionⁱ	747 – 796	DisorderedSequence analysisAdd BLAST	50
Regionⁱ	1060 – 1079	DisorderedSequence analysisAdd BLAST	20
Regionⁱ	1112 – 1143	DisorderedSequence analysisAdd BLAST	32
Regionⁱ	1190 – 1214	DisorderedSequence analysisAdd BLAST	25
Regionⁱ	1227 – 1298	DisorderedSequence analysisAdd BLAST	72

Motif

Feature key	Position(s)	DescriptionActions	Length
Motifⁱ	275 – 281	PxP motif 12 Publications	7
Motifⁱ	1043 – 1052	LIR1 Publication	10
Motifⁱ	1104 – 1106	TQT motif 11 Publication	3
Motifⁱ	1116 – 1118	TQT motif 21 Publication	3
Motifⁱ	1206 – 1212	PxP motif 22 Publications	7

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	266 – 280	Pro residuesSequence analysisAdd BLAST	15
Compositional biasⁱ	343 – 389	Polar residuesSequence analysisAdd BLAST	47
Compositional biasⁱ	480 – 494	Polar residuesSequence analysisAdd BLAST	15
Compositional biasⁱ	590 – 648	Polar residuesSequence analysisAdd BLAST	59
Compositional biasⁱ	656 – 677	Polar residuesSequence analysisAdd BLAST	22
Compositional biasⁱ	747 – 769	Polar residuesSequence analysisAdd BLAST	23
Compositional biasⁱ	1242 – 1260	Pro residuesSequence analysisAdd BLAST	19

Domainⁱ

The PxP motifs mediate interaction with the catalytic subunit of protein phosphatase 2A (PPP2CA).2 Publications

The LIR motif (LC3-interacting region) is required for the interaction with the ATG8 family proteins GABARAP and MAP1LC3B.2 Publications

Sequence similaritiesⁱ

Belongs to the WD repeat AMBRA1 family.Curated

Keywords - Domainⁱ

Repeat, WD repeat

Phylogenomic databases

eggNOGⁱ	KOG0266, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000016223
HOGENOMⁱ	CLU_008882_0_0_1
InParanoidⁱ	Q9C0C7
OMAⁱ	PPAECCQ
Database of Orthologous Groups More...OrthoDBⁱ	488527at2759
PhylomeDBⁱ	Q9C0C7
TreeFamⁱ	TF328981

Family and domain databases

Database of protein disorder More...DisProtⁱ	DP01328
Gene3Dⁱ	2.130.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf
SMARTⁱ	View protein in SMART SM00320, WD40, 3 hits
SUPFAMⁱ	SSF50978, SSF50978, 1 hit
PROSITEⁱ	View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit

Sequences (6+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 6 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 6 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9C0C7-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP 
        60         70         80         90        100
DSPRSTFLLA FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC 
       110        120        130        140        150
VTFHPTISGL IASGCLDGEV RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ 
       160        170        180        190        200
LLLIATANEI HFWDWSRREP FAVVKTASEM ERVRLVRFDP LGHYLLTAIV 
       210        220        230        240        250
NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL HNFLHMLSSR 
       260        270        280        290        300
SSGIQVGEQS TVQDSATPSP PPPPPQPSTE RPRTSAYIRL RQRVSYPTAE 
       310        320        330        340        350
CCQHLGILCL CSRCSGTRVP SLLPHQDSVP PASARATTPS FSFVQTEPFH 
       360        370        380        390        400
PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP TRRSLGGPLS 
       410        420        430        440        450
SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT SASSVSLLSV 
       460        470        480        490        500
LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN NSGSIRHELQ 
       510        520        530        540        550
CDLRRFFLEY DRLQELDQSL SGEAPQTQQA QEMLNNNIES ERPGPSHQPT 
       560        570        580        590        600
PHSSENNSNL SRGHLNRCRA CHNLLTFNND TLRWERTTPN YSSGEASSSW 
       610        620        630        640        650
QVPSSFESVP SSGSQLPPLE RTEGQTPSSS RLELSSSASP QEERTVGVAF 
       660        670        680        690        700
NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR RRLLESSLIS 
       710        720        730        740        750
LSRYDGAGSR EHPIYPDPAR LSPAAYYAQR MIQYLSRRDS IRQRSMRYQQ 
       760        770        780        790        800
NRLRSSTSSS SSDNQGPSVE GTDLEFEDFE DNGDRSRHRA PRNARMSAPS 
       810        820        830        840        850
LGRFVPRRFL LPEYLPYAGI FHERGQPGLA THSSVNRVLA GAVIGDGQSA 
       860        870        880        890        900
VASNIANTTY RLQWWDFTKF DLPEISNASV NVLVQNCKIY NDASCDISAD 
       910        920        930        940        950
GQLLAAFIPS SQRGFPDEGI LAVYSLAPHN LGEMLYTKRF GPNAISVSLS 
       960        970        980        990       1000
PMGRYVMVGL ASRRILLHPS TEHMVAQVFR LQQAHGGETS MRRVFNVLYP 
      1010       1020       1030       1040       1050
MPADQRRHVS INSARWLPEP GLGLAYGTNK GDLVICRPEA LNSGVEYYWD 
      1060       1070       1080       1090       1100
QLNETVFTVH SNSRSSERPG TSRATWRTDR DMGLMNAIGL QPRNPATSVT 
      1110       1120       1130       1140       1150
SQGTQTLALQ LQNAETQTER EVPEPGTAAS GPGEGEGSEY GASGEDALSR 
      1160       1170       1180       1190       1200
IQRLMAEGGM TAVVQREQST TMASMGGFGN NIIVSHRIHR SSQTGTEPGA 
      1210       1220       1230       1240       1250
AHTSSPQPST SRGLLPEAGQ LAERGLSPRT ASWDQPGTPG REPTQPTLPS 
      1260       1270       1280       1290 
SSPVPIPVSL PSAEGPTLHC ELTNNNHLLD GGSSRGDAAG PRGEPRNR

Length:1,298

Mass (Da):142,507

Last modified:January 15, 2008 - v2

Checksum:ⁱ44FE9CDFFFE6811E

GO

Isoform 2 (identifier: Q9C0C7-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
721-780: Missing.

« Hide

        10         20         30         40         50
MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP 
        60         70         80         90        100
DSPRSTFLLA FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC 
       110        120        130        140        150
VTFHPTISGL IASGCLDGEV RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ 
       160        170        180        190        200
LLLIATANEI HFWDWSRREP FAVVKTASEM ERVRLVRFDP LGHYLLTAIV 
       210        220        230        240        250
NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL HNFLHMLSSR 
       260        270        280        290        300
SSGIQVGEQS TVQDSATPSP PPPPPQPSTE RPRTSAYIRL RQRVSYPTAE 
       310        320        330        340        350
CCQHLGILCL CSRCSGTRVP SLLPHQDSVP PASARATTPS FSFVQTEPFH 
       360        370        380        390        400
PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP TRRSLGGPLS 
       410        420        430        440        450
SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT SASSVSLLSV 
       460        470        480        490        500
LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN NSGSIRHELQ 
       510        520        530        540        550
CDLRRFFLEY DRLQELDQSL SGEAPQTQQA QEMLNNNIES ERPGPSHQPT 
       560        570        580        590        600
PHSSENNSNL SRGHLNRCRA CHNLLTFNND TLRWERTTPN YSSGEASSSW 
       610        620        630        640        650
QVPSSFESVP SSGSQLPPLE RTEGQTPSSS RLELSSSASP QEERTVGVAF 
       660        670        680        690        700
NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR RRLLESSLIS 
       710        720        730        740        750
LSRYDGAGSR EHPIYPDPAR DNGDRSRHRA PRNARMSAPS LGRFVPRRFL 
       760        770        780        790        800
LPEYLPYAGI FHERGQPGLA THSSVNRVLA GAVIGDGQSA VASNIANTTY 
       810        820        830        840        850
RLQWWDFTKF DLPEISNASV NVLVQNCKIY NDASCDISAD GQLLAAFIPS 
       860        870        880        890        900
SQRGFPDEGI LAVYSLAPHN LGEMLYTKRF GPNAISVSLS PMGRYVMVGL 
       910        920        930        940        950
ASRRILLHPS TEHMVAQVFR LQQAHGGETS MRRVFNVLYP MPADQRRHVS 
       960        970        980        990       1000
INSARWLPEP GLGLAYGTNK GDLVICRPEA LNSGVEYYWD QLNETVFTVH 
      1010       1020       1030       1040       1050
SNSRSSERPG TSRATWRTDR DMGLMNAIGL QPRNPATSVT SQGTQTLALQ 
      1060       1070       1080       1090       1100
LQNAETQTER EVPEPGTAAS GPGEGEGSEY GASGEDALSR IQRLMAEGGM 
      1110       1120       1130       1140       1150
TAVVQREQST TMASMGGFGN NIIVSHRIHR SSQTGTEPGA AHTSSPQPST 
      1160       1170       1180       1190       1200
SRGLLPEAGQ LAERGLSPRT ASWDQPGTPG REPTQPTLPS SSPVPIPVSL 
      1210       1220       1230 
PSAEGPTLHC ELTNNNHLLD GGSSRGDAAG PRGEPRNR

Show »

Length:1,238

Mass (Da):135,528

Checksum:ⁱEE24A481D1388999

GO

Isoform 3 (identifier: Q9C0C7-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
691-719: Missing.

« Hide

        10         20         30         40         50
MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP 
        60         70         80         90        100
DSPRSTFLLA FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC 
       110        120        130        140        150
VTFHPTISGL IASGCLDGEV RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ 
       160        170        180        190        200
LLLIATANEI HFWDWSRREP FAVVKTASEM ERVRLVRFDP LGHYLLTAIV 
       210        220        230        240        250
NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL HNFLHMLSSR 
       260        270        280        290        300
SSGIQVGEQS TVQDSATPSP PPPPPQPSTE RPRTSAYIRL RQRVSYPTAE 
       310        320        330        340        350
CCQHLGILCL CSRCSGTRVP SLLPHQDSVP PASARATTPS FSFVQTEPFH 
       360        370        380        390        400
PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP TRRSLGGPLS 
       410        420        430        440        450
SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT SASSVSLLSV 
       460        470        480        490        500
LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN NSGSIRHELQ 
       510        520        530        540        550
CDLRRFFLEY DRLQELDQSL SGEAPQTQQA QEMLNNNIES ERPGPSHQPT 
       560        570        580        590        600
PHSSENNSNL SRGHLNRCRA CHNLLTFNND TLRWERTTPN YSSGEASSSW 
       610        620        630        640        650
QVPSSFESVP SSGSQLPPLE RTEGQTPSSS RLELSSSASP QEERTVGVAF 
       660        670        680        690        700
NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR RLSPAAYYAQ 
       710        720        730        740        750
RMIQYLSRRD SIRQRSMRYQ QNRLRSSTSS SSSDNQGPSV EGTDLEFEDF 
       760        770        780        790        800
EDNGDRSRHR APRNARMSAP SLGRFVPRRF LLPEYLPYAG IFHERGQPGL 
       810        820        830        840        850
ATHSSVNRVL AGAVIGDGQS AVASNIANTT YRLQWWDFTK FDLPEISNAS 
       860        870        880        890        900
VNVLVQNCKI YNDASCDISA DGQLLAAFIP SSQRGFPDEG ILAVYSLAPH 
       910        920        930        940        950
NLGEMLYTKR FGPNAISVSL SPMGRYVMVG LASRRILLHP STEHMVAQVF 
       960        970        980        990       1000
RLQQAHGGET SMRRVFNVLY PMPADQRRHV SINSARWLPE PGLGLAYGTN 
      1010       1020       1030       1040       1050
KGDLVICRPE ALNSGVEYYW DQLNETVFTV HSNSRSSERP GTSRATWRTD 
      1060       1070       1080       1090       1100
RDMGLMNAIG LQPRNPATSV TSQGTQTLAL QLQNAETQTE REVPEPGTAA 
      1110       1120       1130       1140       1150
SGPGEGEGSE YGASGEDALS RIQRLMAEGG MTAVVQREQS TTMASMGGFG 
      1160       1170       1180       1190       1200
NNIIVSHRIH RSSQTGTEPG AAHTSSPQPS TSRGLLPEAG QLAERGLSPR 
      1210       1220       1230       1240       1250
TASWDQPGTP GREPTQPTLP SSSPVPIPVS LPSAEGPTLH CELTNNNHLL 
      1260 
DGGSSRGDAA GPRGEPRNR

Show »

Length:1,269

Mass (Da):139,269

Checksum:ⁱC9902123C4EE6199

GO

Isoform 4 (identifier: Q9C0C7-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
255-344: Missing.

« Hide

        10         20         30         40         50
MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP 
        60         70         80         90        100
DSPRSTFLLA FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC 
       110        120        130        140        150
VTFHPTISGL IASGCLDGEV RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ 
       160        170        180        190        200
LLLIATANEI HFWDWSRREP FAVVKTASEM ERVRLVRFDP LGHYLLTAIV 
       210        220        230        240        250
NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL HNFLHMLSSR 
       260        270        280        290        300
SSGIQTEPFH PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP 
       310        320        330        340        350
TRRSLGGPLS SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT 
       360        370        380        390        400
SASSVSLLSV LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN 
       410        420        430        440        450
NSGSIRHELQ CDLRRFFLEY DRLQELDQSL SGEAPQTQQA QEMLNNNIES 
       460        470        480        490        500
ERPGPSHQPT PHSSENNSNL SRGHLNRCRA CHNLLTFNND TLRWERTTPN 
       510        520        530        540        550
YSSGEASSSW QVPSSFESVP SSGSQLPPLE RTEGQTPSSS RLELSSSASP 
       560        570        580        590        600
QEERTVGVAF NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR 
       610        620        630        640        650
RRLLESSLIS LSRYDGAGSR EHPIYPDPAR LSPAAYYAQR MIQYLSRRDS 
       660        670        680        690        700
IRQRSMRYQQ NRLRSSTSSS SSDNQGPSVE GTDLEFEDFE DNGDRSRHRA 
       710        720        730        740        750
PRNARMSAPS LGRFVPRRFL LPEYLPYAGI FHERGQPGLA THSSVNRVLA 
       760        770        780        790        800
GAVIGDGQSA VASNIANTTY RLQWWDFTKF DLPEISNASV NVLVQNCKIY 
       810        820        830        840        850
NDASCDISAD GQLLAAFIPS SQRGFPDEGI LAVYSLAPHN LGEMLYTKRF 
       860        870        880        890        900
GPNAISVSLS PMGRYVMVGL ASRRILLHPS TEHMVAQVFR LQQAHGGETS 
       910        920        930        940        950
MRRVFNVLYP MPADQRRHVS INSARWLPEP GLGLAYGTNK GDLVICRPEA 
       960        970        980        990       1000
LNSGVEYYWD QLNETVFTVH SNSRSSERPG TSRATWRTDR DMGLMNAIGL 
      1010       1020       1030       1040       1050
QPRNPATSVT SQGTQTLALQ LQNAETQTER EVPEPGTAAS GPGEGEGSEY 
      1060       1070       1080       1090       1100
GASGEDALSR IQRLMAEGGM TAVVQREQST TMASMGGFGN NIIVSHRIHR 
      1110       1120       1130       1140       1150
SSQTGTEPGA AHTSSPQPST SRGLLPEAGQ LAERGLSPRT ASWDQPGTPG 
      1160       1170       1180       1190       1200
REPTQPTLPS SSPVPIPVSL PSAEGPTLHC ELTNNNHLLD GGSSRGDAAG 

PRGEPRNR

Show »

Length:1,208

Mass (Da):132,837

Checksum:ⁱ3BA3D281C30318F1

GO

Isoform 5 (identifier: Q9C0C7-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
255-344: Missing.
691-691: R → RRSLALSPRL...ITGVSHRAWP

« Hide

        10         20         30         40         50
MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP 
        60         70         80         90        100
DSPRSTFLLA FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC 
       110        120        130        140        150
VTFHPTISGL IASGCLDGEV RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ 
       160        170        180        190        200
LLLIATANEI HFWDWSRREP FAVVKTASEM ERVRLVRFDP LGHYLLTAIV 
       210        220        230        240        250
NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL HNFLHMLSSR 
       260        270        280        290        300
SSGIQTEPFH PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP 
       310        320        330        340        350
TRRSLGGPLS SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT 
       360        370        380        390        400
SASSVSLLSV LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN 
       410        420        430        440        450
NSGSIRHELQ CDLRRFFLEY DRLQELDQSL SGEAPQTQQA QEMLNNNIES 
       460        470        480        490        500
ERPGPSHQPT PHSSENNSNL SRGHLNRCRA CHNLLTFNND TLRWERTTPN 
       510        520        530        540        550
YSSGEASSSW QVPSSFESVP SSGSQLPPLE RTEGQTPSSS RLELSSSASP 
       560        570        580        590        600
QEERTVGVAF NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR 
       610        620        630        640        650
RRSLALSPRL EYSGAILAHC KLRLPGSCHS PASASQVAGT TGAHHHARLI 
       660        670        680        690        700
FAFLVEMEFH HVSQAGLELL TSGDLPTSAS QSAGITGVSH RAWPRLLESS 
       710        720        730        740        750
LISLSRYDGA GSREHPIYPD PARLSPAAYY AQRMIQYLSR RDSIRQRSMR 
       760        770        780        790        800
YQQNRLRSST SSSSSDNQGP SVEGTDLEFE DFEDNGDRSR HRAPRNARMS 
       810        820        830        840        850
APSLGRFVPR RFLLPEYLPY AGIFHERGQP GLATHSSVNR VLAGAVIGDG 
       860        870        880        890        900
QSAVASNIAN TTYRLQWWDF TKFDLPEISN ASVNVLVQNC KIYNDASCDI 
       910        920        930        940        950
SADGQLLAAF IPSSQRGFPD EGILAVYSLA PHNLGEMLYT KRFGPNAISV 
       960        970        980        990       1000
SLSPMGRYVM VGLASRRILL HPSTEHMVAQ VFRLQQAHGG ETSMRRVFNV 
      1010       1020       1030       1040       1050
LYPMPADQRR HVSINSARWL PEPGLGLAYG TNKGDLVICR PEALNSGVEY 
      1060       1070       1080       1090       1100
YWDQLNETVF TVHSNSRSSE RPGTSRATWR TDRDMGLMNA IGLQPRNPAT 
      1110       1120       1130       1140       1150
SVTSQGTQTL ALQLQNAETQ TEREVPEPGT AASGPGEGEG SEYGASGEDA 
      1160       1170       1180       1190       1200
LSRIQRLMAE GGMTAVVQRE QSTTMASMGG FGNNIIVSHR IHRSSQTGTE 
      1210       1220       1230       1240       1250
PGAAHTSSPQ PSTSRGLLPE AGQLAERGLS PRTASWDQPG TPGREPTQPT 
      1260       1270       1280       1290       1300
LPSSSPVPIP VSLPSAEGPT LHCELTNNNH LLDGGSSRGD AAGPRGEPRN 

R

Show »

Length:1,301

Mass (Da):142,585

Checksum:ⁱ0EF81F37DFCA142D

GO

Isoform 6 (identifier: Q9C0C7-6) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-344: Missing.
     691-719: Missing.
     864-880: WWDFTKFDLPEISNASV → GGGTSLSLTSLKSVMLP
     881-1298: Missing.

« Hide

        10         20         30         40         50
MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP 
        60         70         80         90        100
DSPRSTFLLA FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC 
       110        120        130        140        150
VTFHPTISGL IASGCLDGEV RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ 
       160        170        180        190        200
LLLIATANEI HFWDWSRREP FAVVKTASEM ERVRLVRFDP LGHYLLTAIV 
       210        220        230        240        250
NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL HNFLHMLSSR 
       260        270        280        290        300
SSGIQTEPFH PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP 
       310        320        330        340        350
TRRSLGGPLS SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT 
       360        370        380        390        400
SASSVSLLSV LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN 
       410        420        430        440        450
NSGSIRHELQ CDLRRFFLEY DRLQELDQSL SGEAPQTQQA QEMLNNNIES 
       460        470        480        490        500
ERPGPSHQPT PHSSENNSNL SRGHLNRCRA CHNLLTFNND TLRWERTTPN 
       510        520        530        540        550
YSSGEASSSW QVPSSFESVP SSGSQLPPLE RTEGQTPSSS RLELSSSASP 
       560        570        580        590        600
QEERTVGVAF NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR 
       610        620        630        640        650
RLSPAAYYAQ RMIQYLSRRD SIRQRSMRYQ QNRLRSSTSS SSSDNQGPSV 
       660        670        680        690        700
EGTDLEFEDF EDNGDRSRHR APRNARMSAP SLGRFVPRRF LLPEYLPYAG 
       710        720        730        740        750
IFHERGQPGL ATHSSVNRVL AGAVIGDGQS AVASNIANTT YRLQGGGTSL 
       760 
SLTSLKSVML P

Show »

Length:761

Mass (Da):84,428

Checksum:ⁱ4512BD3DCDD64FE1

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A0A075B6T1	A0A075B6T1_HUMAN	Activating molecule in BECN1-regula... Activating molecule in BECN1-regulated autophagy protein 1	AMBRA1	1,179	Annotation score:
H0YE34	H0YE34_HUMAN	Activating molecule in BECN1-regula... Activating molecule in BECN1-regulated autophagy protein 1	AMBRA1	256	Annotation score:
E9PL55	E9PL55_HUMAN	Activating molecule in BECN1-regula... Activating molecule in BECN1-regulated autophagy protein 1	AMBRA1	24	Annotation score:

Sequence cautionⁱ

Isoform 6:

The sequence AL834190 differs from that shown. Reason: Frameshift.Curated

The sequence BAA91067 differs from that shown. Reason: Frameshift.Curated

The sequence BAB14457 differs from that shown. Reason: Erroneous initiation.Curated

The sequence BAB21827 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	165	W → R in BAA91067 (PubMed:14702039).Curated	1
Sequence conflictⁱ	793	N → K in AL834190 (PubMed:17974005).Curated	1
Sequence conflictⁱ	869	K → E in BAB14457 (PubMed:14702039).Curated	1
Sequence conflictⁱ	983	Q → L in BAB14457 (PubMed:14702039).Curated	1
Sequence conflictⁱ	1105	Q → E in AAH45609 (PubMed:15489334).Curated	1
Sequence conflictⁱ	1268	L → V in AAH45609 (PubMed:15489334).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_085051	80	T → M Found in a child with spina bifida; unknown pathological significance; reduced induction of autophagy. 1 Publication	1
Natural variantⁱVAR_085052	364	L → F Found in a fetus with encephalocele; unknown pathological significance; reduced induction of autophagy. 1 Publication	1
Natural variantⁱVAR_085053	833	S → F Found in a child with spina bifida; unknown pathological significance; reduced induction of autophagy. 1 Publication	1
Natural variantⁱVAR_085054	974	M → V Found in a fetus with encephalocele and spina bifida; unknown pathological significance; reduced induction of autophagy. 1 Publication	1
Natural variantⁱVAR_085055	1043	S → F Found in a fetus with anencephaly and spina bifida; unknown pathological significance; does not impair induction of autophagy. 1 Publication	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_030654	255 – 344	Missing in isoform 4, isoform 5 and isoform 6. 3 PublicationsAdd BLAST	90
Alternative sequenceⁱVSP_030655	691 – 719	Missing in isoform 3 and isoform 6. 2 PublicationsAdd BLAST	29
Alternative sequenceⁱVSP_030656	691	R → RRSLALSPRLEYSGAILAHC KLRLPGSCHSPASASQVAGT TGAHHHARLIFAFLVEMEFH HVSQAGLELLTSGDLPTSAS QSAGITGVSHRAWP in isoform 5. 1 Publication	1
Alternative sequenceⁱVSP_030657	721 – 780	Missing in isoform 2. 1 PublicationAdd BLAST	60
Alternative sequenceⁱVSP_045989	864 – 880	WWDFT…SNASV → GGGTSLSLTSLKSVMLP in isoform 6. 1 PublicationAdd BLAST	17
Alternative sequenceⁱVSP_045990	881 – 1298	Missing in isoform 6. 1 PublicationAdd BLAST	418

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	DQ870924 mRNA Translation: ABI74670.1 AB051523 mRNA Translation: BAB21827.1 Different initiation. AK000301 mRNA Translation: BAA91067.1 Frameshift. AK023197 mRNA Translation: BAB14457.1 Different initiation. AL834190 mRNA No translation available. AC024293 Genomic DNA No translation available. AC115097 Genomic DNA No translation available. AC116021 Genomic DNA No translation available. AC127035 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW67996.1 CH471064 Genomic DNA Translation: EAW67999.1 CH471064 Genomic DNA Translation: EAW68001.1 BC045609 mRNA Translation: AAH45609.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS31475.1 [Q9C0C7-4] CCDS73281.1 [Q9C0C7-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001254711.1, NM_001267782.1 [Q9C0C7-5] NP_001254712.1, NM_001267783.1 NP_001287660.1, NM_001300731.1 [Q9C0C7-2] NP_060219.2, NM_017749.3 [Q9C0C7-4] XP_005253066.1, XM_005253009.3 [Q9C0C7-1] XP_005253068.1, XM_005253011.3 [Q9C0C7-3] XP_005253071.1, XM_005253014.3 XP_006718322.1, XM_006718259.2 [Q9C0C7-1]

Genome annotation databases

Ensemblⁱ	ENST00000314845; ENSP00000318313; ENSG00000110497 [Q9C0C7-4] ENST00000458649; ENSP00000415327; ENSG00000110497 ENST00000528950; ENSP00000433945; ENSG00000110497 [Q9C0C7-3] ENST00000534300; ENSP00000431926; ENSG00000110497 [Q9C0C7-2] ENST00000683756; ENSP00000508322; ENSG00000110497
GeneIDⁱ	55626
KEGGⁱ	hsa:55626
MANE-Selectⁱ	ENST00000683756.1; ENSP00000508322.1; NM_001387011.1; NP_001373940.1
UCSC genome browser More...UCSCⁱ	uc001ncu.3, human [Q9C0C7-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9C0C7-5	AMBRA1 isoform 1		PANTR		601	UniRef100_A0A2J8LHC0

Protein	Similar proteins		Species	Score	Length	Source
Q9C0C7	cDNA FLJ60985		HUMAN		1298	UniRef90_Q9C0C7
AMBRA1 isoform 1		PANTR		601
Isoform 4 of Activating molecule in BECN1-regulated autophagy protein 1		HUMAN		1208
Activating molecule in BECN1-regulated autophagy protein 1		HUMAN		1179
Isoform 5 of Activating molecule in BECN1-regulated autophagy protein 1		HUMAN		1301
+2

Protein	Similar proteins		Species	Score	Length	Source
Q9C0C7	Activating molecule in BECN1-regulated autophagy protein 1A		DANRE		1315	UniRef50_Q9C0C7
cDNA FLJ60985		HUMAN		1298
AMBRA1 isoform 1		PANTR		601
activating molecule in BECN1-regulated autophagy protein 1 isoform X4		ACIJB		1212
Autophagy and beclin 1 regulator 1		COLAP		1269
+956
Q9C0C7-6	Activating molecule in BECN1-regulated autophagy protein 1-like		Sinocyclocheilus anshuiensis		836	UniRef50_Q9C0C7-6
Autophagy and beclin 1 regulator 1		VARKO		859
Activating molecule in BECN1-regulated autophagy protein 1 (Fragment)		NESNO		844
AMRA1 protein (Fragment)		Grantiella picta		847
Autophagy and beclin 1 regulator 1		Muscovy Duck (domestic type)		853
+115

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	DQ870924 mRNA Translation: ABI74670.1 AB051523 mRNA Translation: BAB21827.1 Different initiation. AK000301 mRNA Translation: BAA91067.1 Frameshift. AK023197 mRNA Translation: BAB14457.1 Different initiation. AL834190 mRNA No translation available. AC024293 Genomic DNA No translation available. AC115097 Genomic DNA No translation available. AC116021 Genomic DNA No translation available. AC127035 Genomic DNA No translation available. CH471064 Genomic DNA Translation: EAW67996.1 CH471064 Genomic DNA Translation: EAW67999.1 CH471064 Genomic DNA Translation: EAW68001.1 BC045609 mRNA Translation: AAH45609.1
CCDSⁱ	CCDS31475.1 [Q9C0C7-4] CCDS73281.1 [Q9C0C7-2]
RefSeqⁱ	NP_001254711.1, NM_001267782.1 [Q9C0C7-5] NP_001254712.1, NM_001267783.1 NP_001287660.1, NM_001300731.1 [Q9C0C7-2] NP_060219.2, NM_017749.3 [Q9C0C7-4] XP_005253066.1, XM_005253009.3 [Q9C0C7-1] XP_005253068.1, XM_005253011.3 [Q9C0C7-3] XP_005253071.1, XM_005253014.3 XP_006718322.1, XM_006718259.2 [Q9C0C7-1]

3D structure databases

SMRⁱ	Q9C0C7
ModBaseⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	120765, 233 interactors
DIPⁱ	DIP-53597N
IntActⁱ	Q9C0C7, 53 interactors
MINTⁱ	Q9C0C7
STRINGⁱ	9606.ENSP00000431926

PTM databases

GlyGenⁱ	Q9C0C7, 3 sites, 1 O-linked glycan (3 sites)
iPTMnetⁱ	Q9C0C7
PhosphoSitePlusⁱ	Q9C0C7

Genetic variation databases

BioMutaⁱ	AMBRA1
DMDMⁱ	166215833

Proteomic databases

EPDⁱ	Q9C0C7
jPOSTⁱ	Q9C0C7
MassIVEⁱ	Q9C0C7
MaxQBⁱ	Q9C0C7
PaxDbⁱ	Q9C0C7
PeptideAtlasⁱ	Q9C0C7
PRIDEⁱ	Q9C0C7
ProteomicsDBⁱ	32296 79999 [Q9C0C7-1] 80000 [Q9C0C7-2] 80001 [Q9C0C7-3] 80002 [Q9C0C7-4] 80003 [Q9C0C7-5]

Protocols and materials databases

Antibodypediaⁱ	26331, 431 antibodies from 36 providers
The DNASU plasmid repository More...DNASUⁱ	55626

Genome annotation databases

Ensemblⁱ	ENST00000314845; ENSP00000318313; ENSG00000110497 [Q9C0C7-4] ENST00000458649; ENSP00000415327; ENSG00000110497 ENST00000528950; ENSP00000433945; ENSG00000110497 [Q9C0C7-3] ENST00000534300; ENSP00000431926; ENSG00000110497 [Q9C0C7-2] ENST00000683756; ENSP00000508322; ENSG00000110497
GeneIDⁱ	55626
KEGGⁱ	hsa:55626
MANE-Selectⁱ	ENST00000683756.1; ENSP00000508322.1; NM_001387011.1; NP_001373940.1
UCSCⁱ	uc001ncu.3, human [Q9C0C7-1]

Organism-specific databases

CTDⁱ	55626
DisGeNETⁱ	55626
GeneCardsⁱ	AMBRA1
HGNCⁱ	HGNC:25990, AMBRA1
HPAⁱ	ENSG00000110497, Low tissue specificity
MIMⁱ	611359, gene
neXtProtⁱ	NX_Q9C0C7
OpenTargetsⁱ	ENSG00000110497
PharmGKBⁱ	PA162376307
VEuPathDBⁱ	HostDB:ENSG00000110497
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0266, Eukaryota
GeneTreeⁱ	ENSGT00390000016223
HOGENOMⁱ	CLU_008882_0_0_1
InParanoidⁱ	Q9C0C7
OMAⁱ	PPAECCQ
OrthoDBⁱ	488527at2759
PhylomeDBⁱ	Q9C0C7
TreeFamⁱ	TF328981

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
PathwayCommonsⁱ	Q9C0C7
Reactomeⁱ	R-HSA-1632852, Macroautophagy
SignaLinkⁱ	Q9C0C7
SIGNORⁱ	Q9C0C7

Miscellaneous databases

BioGRID-ORCSⁱ	55626, 108 hits in 1104 CRISPR screens
ChiTaRSⁱ	AMBRA1, human
GenomeRNAiⁱ	55626
Pharosⁱ	Q9C0C7, Tbio
Protein Ontology More...PROⁱ	PR:Q9C0C7
RNActⁱ	Q9C0C7, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000110497, Expressed in stomach and 242 other tissues
ExpressionAtlasⁱ	Q9C0C7, baseline and differential
Genevisibleⁱ	Q9C0C7, HS

Family and domain databases

DisProtⁱ	DP01328
Gene3Dⁱ	2.130.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf
SMARTⁱ	View protein in SMART SM00320, WD40, 3 hits
SUPFAMⁱ	SSF50978, SSF50978, 1 hit
PROSITEⁱ	View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AMRA1_HUMAN
Accessionⁱ	Q9C0C7Primary (citable) accession number: Q9C0C7 Secondary accession number(s): A6XN33 , D3DQP8, G3V193, Q86XD6, Q9H8Z0, Q9NXE7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
	Last sequence update:	January 15, 2008
	Last modified:	February 23, 2022
	This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Reference proteome

Documents

Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM
Human entries with genetic variants
List of human entries with genetic variants
Human variants curated from literature reports
Index of human variants curated from literature reports
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

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UniProtKB - Q9C0C7 (AMRA1_HUMAN)

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Activating molecule in BECN1-regulated autophagy protein 1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

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Keywords - PTMi

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<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Q9C0C7

Isoform 3 [Q9C0C7-3]

GO - Molecular functioni

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<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

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<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

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Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

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Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

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Keywords - Domainⁱ

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Keywords - Coding sequence diversityⁱ

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Keywords - Technical termⁱ