UniProtKB - Q9BZM2 (PA2GF_HUMAN)
Protein
Group IIF secretory phospholipase A2
Gene
PLA2G2F
Organism
Homo sapiens (Human)
Status
Functioni
Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at the sn-2 position of phospholipids (phospholipase A2 activity), the catalytic efficiency decreasing in the following order: phosphatidylglycerols > phosphatidylethanolamines > phosphatidylcholines > phosphatidylserines (PubMed:11112443). May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases (By similarity).By similarity1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotation1 PublicationEC:3.1.1.4PROSITE-ProRule annotation1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H2O = (9Z)-octadecenoate + 1-hexadecanoyl-sn-glycero-3-phospho-L-serine + H+1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Ca2+1 PublicationNote: Binds 1 Ca2+ ion per subunit.1 Publication
pH dependencei
Optimum pH is 7-8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 49 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 51 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 67 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 68 | CalciumBy similarity | 1 | |
Active sitei | 114 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium-dependent phospholipase A2 activity Source: UniProtKB
- calcium ion binding Source: GO_Central
- phospholipase A2 activity Source: UniProtKB
- phospholipase A2 activity (consuming 1,2-dipalmitoylphosphatidylcholine) Source: UniProtKB-EC
- phospholipase A2 activity consuming 1,2-dioleoylphosphatidylethanolamine) Source: UniProtKB-EC
- phospholipid binding Source: GO_Central
GO - Biological processi
- arachidonic acid metabolic process Source: Ensembl
- arachidonic acid secretion Source: InterPro
- innate immune response Source: UniProtKB-KW
- lipid catabolic process Source: UniProtKB-KW
- negative regulation of T cell proliferation Source: GO_Central
- phosphatidic acid biosynthetic process Source: Reactome
- phosphatidylcholine acyl-chain remodeling Source: UniProtKB
- phosphatidylethanolamine acyl-chain remodeling Source: UniProtKB
- phosphatidylglycerol acyl-chain remodeling Source: UniProtKB
- phosphatidylinositol acyl-chain remodeling Source: Reactome
- phosphatidylserine acyl-chain remodeling Source: UniProtKB
- phospholipid metabolic process Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Immunity, Innate immunity, Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9BZM2 |
Reactomei | R-HSA-1482788, Acyl chain remodelling of PC R-HSA-1482801, Acyl chain remodelling of PS R-HSA-1482839, Acyl chain remodelling of PE R-HSA-1482922, Acyl chain remodelling of PI R-HSA-1482925, Acyl chain remodelling of PG R-HSA-1483166, Synthesis of PA |
Chemistry databases
SwissLipidsi | SLP:000000654 [Q9BZM2-1] |
Names & Taxonomyi
Protein namesi | Recommended name: Group IIF secretory phospholipase A21 Publication (EC:3.1.1.41 Publication)Short name: GIIF sPLA2 Short name: sPLA2-IIF Alternative name(s): Phosphatidylcholine 2-acylhydrolase 2F |
Gene namesi | Name:PLA2G2F |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30040, PLA2G2F |
neXtProti | NX_Q9BZM2 |
VEuPathDBi | HostDB:ENSG00000158786.4 |
Subcellular locationi
Plasma membrane
- Cell membrane ; Peripheral membrane protein By similarity
Extracellular region or secreted
- Secreted By similarity
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular region Source: Reactome
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Membrane, SecretedPathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000158786 |
PharmGKBi | PA134931043 |
Miscellaneous databases
Pharosi | Q9BZM2, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4278 |
GuidetoPHARMACOLOGYi | 1420 |
Genetic variation databases
BioMutai | PLA2G2F |
DMDMi | 20139134 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000022759 | 21 – 168 | Group IIF secretory phospholipase A2Add BLAST | 148 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 46 ↔ 138 | By similarity | ||
Disulfide bondi | 48 ↔ 64 | By similarity | ||
Disulfide bondi | 63 ↔ 120 | By similarity | ||
Disulfide bondi | 69 ↔ 145 | By similarity | ||
Disulfide bondi | 70 ↔ 113 | By similarity | ||
Disulfide bondi | 79 ↔ 106 | By similarity | ||
Glycosylationi | 92 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 98 ↔ 111 | By similarity | ||
Glycosylationi | 102 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 123 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 144 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9BZM2 |
PRIDEi | Q9BZM2 |
ProteomicsDBi | 79874 [Q9BZM2-1] 79875 [Q9BZM2-2] |
PTM databases
GlyGeni | Q9BZM2, 4 sites |
iPTMneti | Q9BZM2 |
PhosphoSitePlusi | Q9BZM2 |
Expressioni
Tissue specificityi
Expressed at high levels in placenta, testis, thymus and at lower levels in heart, kidney, liver and prostate (PubMed:11112443). Highly expressed in rheumatoid arthritic tissues, including synovial lining cells in the intima, capillary endothelial cells and plasma cells (PubMed:11877435).2 Publications
Gene expression databases
Bgeei | ENSG00000158786, Expressed in urinary bladder and 33 other tissues |
Genevisiblei | Q9BZM2, HS |
Organism-specific databases
HPAi | ENSG00000158786, Tissue enhanced (lymphoid tissue, skin, urinary bladder) |
Interactioni
Binary interactionsi
Hide detailsIsoform 2 [Q9BZM2-2]
With | #Exp. | IntAct |
---|---|---|
NBL1 - isoform 2 [P41271-2] | 3 | EBI-12826629,EBI-12135485 |
VENTX [O95231] | 3 | EBI-12826629,EBI-10191303 |
Protein-protein interaction databases
BioGRIDi | 122219, 3 interactors |
IntActi | Q9BZM2, 2 interactors |
STRINGi | 9606.ENSP00000364243 |
Chemistry databases
BindingDBi | Q9BZM2 |
Miscellaneous databases
RNActi | Q9BZM2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 139 – 168 | Required for localization on the plasma membraneBy similarityAdd BLAST | 30 |
Sequence similaritiesi
Belongs to the phospholipase A2 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4087, Eukaryota |
GeneTreei | ENSGT00940000161819 |
HOGENOMi | CLU_090683_2_0_1 |
InParanoidi | Q9BZM2 |
OMAi | ETECDKQ |
OrthoDBi | 1422829at2759 |
PhylomeDBi | Q9BZM2 |
TreeFami | TF319283 |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00118, PA2_HIS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9BZM2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKKFFTVAIL AGSVLSTAHG SLLNLKAMVE AVTGRSAILS FVGYGCYCGL
60 70 80 90 100
GGRGQPKDEV DWCCHAHDCC YQELFDQGCH PYVDHYDHTI ENNTEIVCSD
110 120 130 140 150
LNKTECDKQT CMCDKNMVLC LMNQTYREEY RGFLNVYCQG PTPNCSIYEP
160
PPEEVTCSHQ SPAPPAPP
Isoform 2 (identifier: Q9BZM2-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-1: M → MADGAKANPKGFKKKVLDRCFSGWRGPRFGASCPSRTSRSSLGM
Note: No signal peptide could be predicted in this isoform, challenging its subcellular location within the secretory pathway and hence the formation of disulfide bonds, which are required for its activity.Curated
Show »Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 149 | E → G in BAC04210 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037524 | 1 | M → MADGAKANPKGFKKKVLDRC FSGWRGPRFGASCPSRTSRS SLGM in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF306566 mRNA Translation: AAG50242.1 AK093645 mRNA Translation: BAC04210.1 AL158172 Genomic DNA No translation available. Z98257 Genomic DNA No translation available. |
CCDSi | CCDS204.2 [Q9BZM2-2] |
RefSeqi | NP_073730.3, NM_022819.3 [Q9BZM2-2] XP_011540258.1, XM_011541956.1 [Q9BZM2-2] |
Genome annotation databases
Ensembli | ENST00000375102; ENSP00000364243; ENSG00000158786 [Q9BZM2-2] |
GeneIDi | 64600 |
KEGGi | hsa:64600 |
UCSCi | uc009vpp.2, human [Q9BZM2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF306566 mRNA Translation: AAG50242.1 AK093645 mRNA Translation: BAC04210.1 AL158172 Genomic DNA No translation available. Z98257 Genomic DNA No translation available. |
CCDSi | CCDS204.2 [Q9BZM2-2] |
RefSeqi | NP_073730.3, NM_022819.3 [Q9BZM2-2] XP_011540258.1, XM_011541956.1 [Q9BZM2-2] |
3D structure databases
SMRi | Q9BZM2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122219, 3 interactors |
IntActi | Q9BZM2, 2 interactors |
STRINGi | 9606.ENSP00000364243 |
Chemistry databases
BindingDBi | Q9BZM2 |
ChEMBLi | CHEMBL4278 |
GuidetoPHARMACOLOGYi | 1420 |
SwissLipidsi | SLP:000000654 [Q9BZM2-1] |
PTM databases
GlyGeni | Q9BZM2, 4 sites |
iPTMneti | Q9BZM2 |
PhosphoSitePlusi | Q9BZM2 |
Genetic variation databases
BioMutai | PLA2G2F |
DMDMi | 20139134 |
Proteomic databases
PaxDbi | Q9BZM2 |
PRIDEi | Q9BZM2 |
ProteomicsDBi | 79874 [Q9BZM2-1] 79875 [Q9BZM2-2] |
Protocols and materials databases
Antibodypediai | 68368, 4 antibodies |
DNASUi | 64600 |
Genome annotation databases
Ensembli | ENST00000375102; ENSP00000364243; ENSG00000158786 [Q9BZM2-2] |
GeneIDi | 64600 |
KEGGi | hsa:64600 |
UCSCi | uc009vpp.2, human [Q9BZM2-1] |
Organism-specific databases
CTDi | 64600 |
GeneCardsi | PLA2G2F |
HGNCi | HGNC:30040, PLA2G2F |
HPAi | ENSG00000158786, Tissue enhanced (lymphoid tissue, skin, urinary bladder) |
neXtProti | NX_Q9BZM2 |
OpenTargetsi | ENSG00000158786 |
PharmGKBi | PA134931043 |
VEuPathDBi | HostDB:ENSG00000158786.4 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4087, Eukaryota |
GeneTreei | ENSGT00940000161819 |
HOGENOMi | CLU_090683_2_0_1 |
InParanoidi | Q9BZM2 |
OMAi | ETECDKQ |
OrthoDBi | 1422829at2759 |
PhylomeDBi | Q9BZM2 |
TreeFami | TF319283 |
Enzyme and pathway databases
PathwayCommonsi | Q9BZM2 |
Reactomei | R-HSA-1482788, Acyl chain remodelling of PC R-HSA-1482801, Acyl chain remodelling of PS R-HSA-1482839, Acyl chain remodelling of PE R-HSA-1482922, Acyl chain remodelling of PI R-HSA-1482925, Acyl chain remodelling of PG R-HSA-1483166, Synthesis of PA |
Miscellaneous databases
BioGRID-ORCSi | 64600, 2 hits in 980 CRISPR screens |
GenomeRNAii | 64600 |
Pharosi | Q9BZM2, Tchem |
PROi | PR:Q9BZM2 |
RNActi | Q9BZM2, protein |
Gene expression databases
Bgeei | ENSG00000158786, Expressed in urinary bladder and 33 other tissues |
Genevisiblei | Q9BZM2, HS |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00118, PA2_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2GF_HUMAN | |
Accessioni | Q9BZM2Primary (citable) accession number: Q9BZM2 Secondary accession number(s): Q5R385, Q8N217, Q9H506 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 11, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - SIMILARITY comments
Index of protein domains and families