TPSD1

Functionⁱ

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.By similarity

Caution

Although PubMed:11174199 reported this as a pseudogene, PubMed:12391231 showed it is expressed and has proteolytic activity when expressed in bacterial cells.Curated

Catalytic activityⁱ

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	81	Charge relay systemBy similarity	1
Active siteⁱ	128	Charge relay systemBy similarity	1
Active siteⁱ	231	Charge relay systemBy similarity	1

GO - Molecular functionⁱ

serine-type endopeptidase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
serine-type peptidase activity
Source: ProtInc
Traceable author statementⁱ
- 9920877

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

proteolysis
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Serine protease

Molecular function

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	S01.054

MEROPSⁱ

S01.054

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Tryptase delta (EC:3.4.21.59) Alternative name(s): Delta-tryptase HmMCP-3-like tryptase III Mast cell mMCP-7-like Tryptase-3
Gene namesⁱ	Name:TPSD1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 16

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000095917.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:14118 TPSD1
MIMⁱ	609272 gene
neXtProtⁱ	NX_Q9BZJ3

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	23430
Open Targets More...OpenTargetsⁱ	ENSG00000095917
PharmGKBⁱ	PA37845

Chemistry databases

ChEMBLⁱ	CHEMBL2095193

ChEMBLⁱ

CHEMBL2095193

Polymorphism and mutation databases

BioMutaⁱ	TPSD1
DMDMⁱ	239938722

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
Signal peptideⁱ	1 – 25	Sequence analysisAdd BLAST	25
PropeptideⁱPRO_0000027483	26 – 37	Activation peptideBy similarityAdd BLAST	12
ChainⁱPRO_0000027484	38 – 242	Tryptase deltaAdd BLAST	205

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	66 ↔ 82	PROSITE-ProRule annotation
Glycosylationⁱ	139	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	162 ↔ 237	PROSITE-ProRule annotation
Disulfide bondⁱ	195 ↔ 218	PROSITE-ProRule annotation

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbⁱ	Q9BZJ3
PeptideAtlas More...PeptideAtlasⁱ	Q9BZJ3
PRIDEⁱ	Q9BZJ3
ProteomicsDBⁱ	79856 79857 [Q9BZJ3-2]

PTM databases

iPTMnetⁱ	Q9BZJ3
PhosphoSitePlusⁱ	Q9BZJ3

Expressionⁱ

Tissue specificityⁱ

Expressed in colon, lung, heart and synovial tissue. May be specific to mast cells.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000095917 Expressed in 84 organ(s), highest expression level in esophagogastric junction muscularis propria
CleanExⁱ	HS_TPSD1
ExpressionAtlasⁱ	Q9BZJ3 baseline and differential
Genevisibleⁱ	Q9BZJ3 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB002215 CAB032871 HPA040182 HPA049554

HPAⁱ

CAB002215
CAB032871
HPA040182
HPA049554

Interactionⁱ

Subunit structureⁱ

Homotetramer.By similarity

Protein-protein interaction databases

STRINGⁱ	9606.ENSP00000211076

STRINGⁱ

9606.ENSP00000211076

Chemistry databases

BindingDBⁱ

Q9BZJ3

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9BZJ3
SMRⁱ	Q9BZJ3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	38 – 242	Peptidase S1PROSITE-ProRule annotationAdd BLAST		205

Sequence similaritiesⁱ

Belongs to the peptidase S1 family. Tryptase subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG3627 Eukaryota COG5640 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140944
HOVERGENⁱ	HBG013304
InParanoidⁱ	Q9BZJ3
KEGG Orthology (KO) More...KOⁱ	K01340
OMAⁱ	WINEHIF
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0AH5
PhylomeDBⁱ	Q9BZJ3
TreeFamⁱ	TF351676

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00190 Tryp_SPc, 1 hit
InterProⁱ	View protein in InterPro IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00089 Trypsin, 1 hit
PRINTSⁱ	PR00722 CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00020 Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494 SSF50494, 1 hit
PROSITEⁱ	View protein in PROSITE PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: Q9BZJ3-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLAPQMLS LLLLALPVLA SPAYVAPAPG QALQQTGIVG GQEAPRSKWP 
        60         70         80         90        100
WQVSLRVRGP YWMHFCGGSL IHPQWVLTAA HCVEPDIKDL AALRVQLREQ 
       110        120        130        140        150
HLYYQDQLLP VSRIIVHPQF YIIQTGADIA LLELEEPVNI SSHIHTVTLP 
       160        170        180        190        200
PASETFPPGM PCWVTGWGDV DNNVHLPPPY PLKEVEVPVV ENHLCNAEYH 
       210        220        230        240 
TGLHTGHSFQ IVRDDMLCAG SENHDSCQGD SGGPLVCKVN GT

Length:242

Mass (Da):26,584

Last modified:June 16, 2009 - v2

Checksum:ⁱ6E72D8AE1EDEA2F1

GO

Isoform 2 (identifier: Q9BZJ3-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
86-94: Missing.

« Hide

        10         20         30         40         50
MLLLAPQMLS LLLLALPVLA SPAYVAPAPG QALQQTGIVG GQEAPRSKWP 
        60         70         80         90        100
WQVSLRVRGP YWMHFCGGSL IHPQWVLTAA HCVEPVQLRE QHLYYQDQLL 
       110        120        130        140        150
PVSRIIVHPQ FYIIQTGADI ALLELEEPVN ISSHIHTVTL PPASETFPPG 
       160        170        180        190        200
MPCWVTGWGD VDNNVHLPPP YPLKEVEVPV VENHLCNAEY HTGLHTGHSF 
       210        220        230 
QIVRDDMLCA GSENHDSCQG DSGGPLVCKV NGT

Show »

Length:233

Mass (Da):25,587

Checksum:ⁱ74A9069BFC39CF44

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A0C4DFZ7	A0A0C4DFZ7_HUMAN		Tryptase delta Tryptase delta	TPSD1	235	Annotation score:

Sequence cautionⁱ

The sequence AAD17861 differs from that shown. Reason: Erroneous initiation.Curated

The sequence AAK12909 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	25	V → G in AAD17861 (PubMed:9920877).Curated		1
Sequence conflictⁱ	178	P → S in AAL17874 (PubMed:12391231).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_016870	22	P → R1 PublicationCorresponds to variant dbSNP:rs3865205Ensembl.	1
Natural variantⁱVAR_016871	25	V → A. Corresponds to variant dbSNP:rs1800984Ensembl.	1
Natural variantⁱVAR_016872	83	V → M3 PublicationsCorresponds to variant dbSNP:rs1141967Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_008319	86 – 94	Missing in isoform 2. 1 Publication		9

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF098327 Genomic DNA Translation: AAD17845.1 AF099147 Genomic DNA Translation: AAD17861.1 Different initiation. AF318074 Genomic DNA Translation: AAK12909.1 Different initiation. AE006466 Genomic DNA Translation: AAK61272.1 AC120498 Genomic DNA No translation available. AF421357 mRNA Translation: AAL86695.1 AF206664 mRNA Translation: AAG35694.1 AY055427 mRNA Translation: AAL17874.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS10432.1 [Q9BZJ3-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_036349.1, NM_012217.2 [Q9BZJ3-1]
UniGeneⁱ	Hs.677789

Genome annotation databases

Ensemblⁱ	ENST00000211076; ENSP00000211076; ENSG00000095917 [Q9BZJ3-1]
GeneIDⁱ	23430
KEGGⁱ	hsa:23430
UCSC genome browser More...UCSCⁱ	uc002clb.2 human [Q9BZJ3-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9BZJ3	Tryptase delta		HUMAN		235	UniRef100_Q9BZJ3

Protein	Similar proteins		Species	Score	Length	Source
Q9BZJ3	Tryptase delta		HUMAN		235	UniRef90_Q9BZJ3
Uncharacterized protein		PONAB		282
Uncharacterized protein		CHLSB		282
Uncharacterized protein		MANLE		282
Uncharacterized protein		MACFA		282
+16

Protein	Similar proteins		Species	Score	Length	Source
Q9BZJ3	Tryptase alpha/beta-1	)	HUMAN		275	UniRef50_Q15661
Tryptase beta-2		MOUSE		276
Tryptase beta-2		RAT		274
TPSB2 isoform 1		PANTR		292
Uncharacterized protein		PANPA		282
+143

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF098327 Genomic DNA Translation: AAD17845.1 AF099147 Genomic DNA Translation: AAD17861.1 Different initiation. AF318074 Genomic DNA Translation: AAK12909.1 Different initiation. AE006466 Genomic DNA Translation: AAK61272.1 AC120498 Genomic DNA No translation available. AF421357 mRNA Translation: AAL86695.1 AF206664 mRNA Translation: AAG35694.1 AY055427 mRNA Translation: AAL17874.1
CCDSⁱ	CCDS10432.1 [Q9BZJ3-1]
RefSeqⁱ	NP_036349.1, NM_012217.2 [Q9BZJ3-1]
UniGeneⁱ	Hs.677789

3D structure databases

ProteinModelPortalⁱ	Q9BZJ3
SMRⁱ	Q9BZJ3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	9606.ENSP00000211076

STRINGⁱ

9606.ENSP00000211076

Chemistry databases

BindingDBⁱ	Q9BZJ3
ChEMBLⁱ	CHEMBL2095193

Protein family/group databases

MEROPSⁱ	S01.054

MEROPSⁱ

S01.054

PTM databases

iPTMnetⁱ	Q9BZJ3
PhosphoSitePlusⁱ	Q9BZJ3

Polymorphism and mutation databases

BioMutaⁱ	TPSD1
DMDMⁱ	239938722

Proteomic databases

PaxDbⁱ	Q9BZJ3
PeptideAtlasⁱ	Q9BZJ3
PRIDEⁱ	Q9BZJ3
ProteomicsDBⁱ	79856 79857 [Q9BZJ3-2]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000211076; ENSP00000211076; ENSG00000095917 [Q9BZJ3-1]
GeneIDⁱ	23430
KEGGⁱ	hsa:23430
UCSCⁱ	uc002clb.2 human [Q9BZJ3-1]

Organism-specific databases

CTDⁱ	23430
DisGeNETⁱ	23430
EuPathDBⁱ	HostDB:ENSG00000095917.13
GeneCardsⁱ	TPSD1
H-InvDBⁱ	HIX0038551
HGNCⁱ	HGNC:14118 TPSD1
HPAⁱ	CAB002215 CAB032871 HPA040182 HPA049554
MIMⁱ	609272 gene
neXtProtⁱ	NX_Q9BZJ3
OpenTargetsⁱ	ENSG00000095917
PharmGKBⁱ	PA37845
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3627 Eukaryota COG5640 LUCA
GeneTreeⁱ	ENSGT00900000140944
HOVERGENⁱ	HBG013304
InParanoidⁱ	Q9BZJ3
KOⁱ	K01340
OMAⁱ	WINEHIF
OrthoDBⁱ	EOG091G0AH5
PhylomeDBⁱ	Q9BZJ3
TreeFamⁱ	TF351676

Miscellaneous databases

GeneWikiⁱ	TPSD1
GenomeRNAiⁱ	23430
Protein Ontology More...PROⁱ	PR:Q9BZJ3
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000095917 Expressed in 84 organ(s), highest expression level in esophagogastric junction muscularis propria
CleanExⁱ	HS_TPSD1
ExpressionAtlasⁱ	Q9BZJ3 baseline and differential
Genevisibleⁱ	Q9BZJ3 HS

Family and domain databases

CDDⁱ	cd00190 Tryp_SPc, 1 hit
InterProⁱ	View protein in InterPro IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER
Pfamⁱ	View protein in Pfam PF00089 Trypsin, 1 hit
PRINTSⁱ	PR00722 CHYMOTRYPSIN
SMARTⁱ	View protein in SMART SM00020 Tryp_SPc, 1 hit
SUPFAMⁱ	SSF50494 SSF50494, 1 hit
PROSITEⁱ	View protein in PROSITE PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TRYD_HUMAN
Accessionⁱ	Q9BZJ3Primary (citable) accession number: Q9BZJ3 Secondary accession number(s): O95824 , Q8TDI6, Q96L36, Q96RZ5, Q9H2Y6, Q9UQI8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
	Last sequence update:	June 16, 2009
	Last modified:	November 7, 2018
	This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Peptidase families
Classification of peptidase families and list of entries
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9BZJ3 (TRYD_HUMAN)

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Tryptase delta

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ