UniProtKB - Q9BZJ3 (TRYD_HUMAN)
Tryptase delta
TPSD1
Functioni
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
By similarityCaution
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.1 Publication EC:3.4.21.59
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 81 | Charge relay systemBy similarity | 1 | |
Active sitei | 128 | Charge relay systemBy similarity | 1 | |
Active sitei | 231 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: GO_Central
- serine-type peptidase activity Source: ProtInc
GO - Biological processi
- proteolysis Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
PathwayCommonsi | Q9BZJ3 |
Protein family/group databases
MEROPSi | S01.054 |
Names & Taxonomyi
Protein namesi | Recommended name: Tryptase delta (EC:3.4.21.59)Alternative name(s): Delta-tryptase HmMCP-3-like tryptase III Mast cell mMCP-7-like Tryptase-3 |
Gene namesi | Name:TPSD1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14118, TPSD1 |
MIMi | 609272, gene |
neXtProti | NX_Q9BZJ3 |
VEuPathDBi | HostDB:ENSG00000095917 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Note: Released from the secretory granules upon mast cell activation.By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 23430 |
OpenTargetsi | ENSG00000095917 |
PharmGKBi | PA37845 |
Miscellaneous databases
Pharosi | Q9BZJ3, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2095193 |
Genetic variation databases
BioMutai | TPSD1 |
DMDMi | 239938722 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | Sequence analysisAdd BLAST | 25 | |
PropeptideiPRO_0000027483 | 26 – 37 | Activation peptideBy similarityAdd BLAST | 12 | |
ChainiPRO_0000027484 | 38 – 242 | Tryptase deltaAdd BLAST | 205 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 66 ↔ 82 | PROSITE-ProRule annotation | ||
Glycosylationi | 139 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 162 ↔ 237 | PROSITE-ProRule annotation | ||
Disulfide bondi | 195 ↔ 218 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
jPOSTi | Q9BZJ3 |
MassIVEi | Q9BZJ3 |
PaxDbi | Q9BZJ3 |
PeptideAtlasi | Q9BZJ3 |
PRIDEi | Q9BZJ3 |
ProteomicsDBi | 79856 [Q9BZJ3-1] 79857 [Q9BZJ3-2] |
PTM databases
GlyGeni | Q9BZJ3, 1 site |
iPTMneti | Q9BZJ3 |
PhosphoSitePlusi | Q9BZJ3 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000095917, Expressed in esophagogastric junction muscularis propria and 102 other tissues |
ExpressionAtlasi | Q9BZJ3, baseline and differential |
Genevisiblei | Q9BZJ3, HS |
Organism-specific databases
HPAi | ENSG00000095917, Tissue enhanced (stomach) |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 9606.ENSP00000211076 |
Chemistry databases
BindingDBi | Q9BZJ3 |
Miscellaneous databases
RNActi | Q9BZJ3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 242 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 205 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000165532 |
HOGENOMi | CLU_006842_1_2_1 |
InParanoidi | Q9BZJ3 |
OMAi | NQLCDAE |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9BZJ3 |
TreeFami | TF351676 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLLLAPQMLS LLLLALPVLA SPAYVAPAPG QALQQTGIVG GQEAPRSKWP
60 70 80 90 100
WQVSLRVRGP YWMHFCGGSL IHPQWVLTAA HCVEPDIKDL AALRVQLREQ
110 120 130 140 150
HLYYQDQLLP VSRIIVHPQF YIIQTGADIA LLELEEPVNI SSHIHTVTLP
160 170 180 190 200
PASETFPPGM PCWVTGWGDV DNNVHLPPPY PLKEVEVPVV ENHLCNAEYH
210 220 230 240
TGLHTGHSFQ IVRDDMLCAG SENHDSCQGD SGGPLVCKVN GT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DFZ7 | A0A0C4DFZ7_HUMAN | Tryptase delta | TPSD1 | 235 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | V → G in AAD17861 (PubMed:9920877).Curated | 1 | |
Sequence conflicti | 178 | P → S in AAL17874 (PubMed:12391231).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_016870 | 22 | P → R1 PublicationCorresponds to variant dbSNP:rs3865205Ensembl. | 1 | |
Natural variantiVAR_016871 | 25 | V → A. Corresponds to variant dbSNP:rs1800984Ensembl. | 1 | |
Natural variantiVAR_016872 | 83 | V → M3 PublicationsCorresponds to variant dbSNP:rs1141967Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008319 | 86 – 94 | Missing in isoform 2. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF098327 Genomic DNA Translation: AAD17845.1 AF099147 Genomic DNA Translation: AAD17861.1 Different initiation. AF318074 Genomic DNA Translation: AAK12909.1 Different initiation. AE006466 Genomic DNA Translation: AAK61272.1 AC120498 Genomic DNA No translation available. AF421357 mRNA Translation: AAL86695.1 AF206664 mRNA Translation: AAG35694.1 AY055427 mRNA Translation: AAL17874.1 |
CCDSi | CCDS10432.1 [Q9BZJ3-1] |
RefSeqi | NP_036349.1, NM_012217.2 [Q9BZJ3-1] |
Genome annotation databases
Ensembli | ENST00000211076.5; ENSP00000211076.3; ENSG00000095917.14 |
GeneIDi | 23430 |
KEGGi | hsa:23430 |
MANE-Selecti | ENST00000211076.5; ENSP00000211076.3; NM_012217.3; NP_036349.1 |
UCSCi | uc002clb.2, human [Q9BZJ3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF098327 Genomic DNA Translation: AAD17845.1 AF099147 Genomic DNA Translation: AAD17861.1 Different initiation. AF318074 Genomic DNA Translation: AAK12909.1 Different initiation. AE006466 Genomic DNA Translation: AAK61272.1 AC120498 Genomic DNA No translation available. AF421357 mRNA Translation: AAL86695.1 AF206664 mRNA Translation: AAG35694.1 AY055427 mRNA Translation: AAL17874.1 |
CCDSi | CCDS10432.1 [Q9BZJ3-1] |
RefSeqi | NP_036349.1, NM_012217.2 [Q9BZJ3-1] |
3D structure databases
AlphaFoldDBi | Q9BZJ3 |
SMRi | Q9BZJ3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9606.ENSP00000211076 |
Chemistry databases
BindingDBi | Q9BZJ3 |
ChEMBLi | CHEMBL2095193 |
Protein family/group databases
MEROPSi | S01.054 |
PTM databases
GlyGeni | Q9BZJ3, 1 site |
iPTMneti | Q9BZJ3 |
PhosphoSitePlusi | Q9BZJ3 |
Genetic variation databases
BioMutai | TPSD1 |
DMDMi | 239938722 |
Proteomic databases
jPOSTi | Q9BZJ3 |
MassIVEi | Q9BZJ3 |
PaxDbi | Q9BZJ3 |
PeptideAtlasi | Q9BZJ3 |
PRIDEi | Q9BZJ3 |
ProteomicsDBi | 79856 [Q9BZJ3-1] 79857 [Q9BZJ3-2] |
Protocols and materials databases
Antibodypediai | 55908, 104 antibodies from 22 providers |
DNASUi | 23430 |
Genome annotation databases
Ensembli | ENST00000211076.5; ENSP00000211076.3; ENSG00000095917.14 |
GeneIDi | 23430 |
KEGGi | hsa:23430 |
MANE-Selecti | ENST00000211076.5; ENSP00000211076.3; NM_012217.3; NP_036349.1 |
UCSCi | uc002clb.2, human [Q9BZJ3-1] |
Organism-specific databases
CTDi | 23430 |
DisGeNETi | 23430 |
GeneCardsi | TPSD1 |
HGNCi | HGNC:14118, TPSD1 |
HPAi | ENSG00000095917, Tissue enhanced (stomach) |
MIMi | 609272, gene |
neXtProti | NX_Q9BZJ3 |
OpenTargetsi | ENSG00000095917 |
PharmGKBi | PA37845 |
VEuPathDBi | HostDB:ENSG00000095917 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000165532 |
HOGENOMi | CLU_006842_1_2_1 |
InParanoidi | Q9BZJ3 |
OMAi | NQLCDAE |
OrthoDBi | 1314811at2759 |
PhylomeDBi | Q9BZJ3 |
TreeFami | TF351676 |
Enzyme and pathway databases
PathwayCommonsi | Q9BZJ3 |
Miscellaneous databases
BioGRID-ORCSi | 23430, 15 hits in 1063 CRISPR screens |
GeneWikii | TPSD1 |
GenomeRNAii | 23430 |
Pharosi | Q9BZJ3, Tbio |
PROi | PR:Q9BZJ3 |
RNActi | Q9BZJ3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000095917, Expressed in esophagogastric junction muscularis propria and 102 other tissues |
ExpressionAtlasi | Q9BZJ3, baseline and differential |
Genevisiblei | Q9BZJ3, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TRYD_HUMAN | |
Accessioni | Q9BZJ3Primary (citable) accession number: Q9BZJ3 Secondary accession number(s): O95824 Q9UQI8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | June 16, 2009 | |
Last modified: | May 25, 2022 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families