UniProtKB - Q9BZJ0 (CRNL1_HUMAN)
Crooked neck-like protein 1
CRNKL1
Functioni
Involved in pre-mRNA splicing process.
4 PublicationsGO - Molecular functioni
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- spliceosomal complex assembly Source: UniProtKB
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | Q9BZJ0 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway |
SignaLinki | Q9BZJ0 |
Names & Taxonomyi
Protein namesi | Recommended name: Crooked neck-like protein 1Alternative name(s): Crooked neck homolog Short name: hCrn |
Gene namesi | Name:CRNKL1 Synonyms:CRN ORF Names:CGI-201, MSTP021 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15762, CRNKL1 |
MIMi | 610952, gene |
neXtProti | NX_Q9BZJ0 |
VEuPathDBi | HostDB:ENSG00000101343 |
Subcellular locationi
Nucleus
- Nucleus 4 Publications
- Nucleus speckle 1 Publication
Note: Colocalizes with core spliceosomal snRNP proteins (PubMed:12084575).1 Publication
Nucleus
- catalytic step 2 spliceosome Source: UniProtKB
- nuclear speck Source: UniProtKB-SubCell
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- post-mRNA release spliceosomal complex Source: GO_Central
- spliceosomal complex Source: UniProtKB
- U2-type catalytic step 2 spliceosome Source: UniProtKB
Other locations
- Prp19 complex Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 51340 |
OpenTargetsi | ENSG00000101343 |
PharmGKBi | PA26886 |
Miscellaneous databases
Pharosi | Q9BZJ0, Tbio |
Genetic variation databases
BioMutai | CRNKL1 |
DMDMi | 147744555 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
ChainiPRO_0000205719 | 1 – 848 | Crooked neck-like protein 1Add BLAST | 848 | ||
Isoform 2 (identifier: Q9BZJ0-2) | |||||
Initiator methioninei | RemovedCombined sources |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 503 | PhosphoserineCombined sources | 1 | ||
Isoform 2 (identifier: Q9BZJ0-2) | |||||
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9BZJ0 |
jPOSTi | Q9BZJ0 |
MassIVEi | Q9BZJ0 |
MaxQBi | Q9BZJ0 |
PaxDbi | Q9BZJ0 |
PeptideAtlasi | Q9BZJ0 |
PRIDEi | Q9BZJ0 |
ProteomicsDBi | 79851 [Q9BZJ0-1] 79852 [Q9BZJ0-2] 79853 [Q9BZJ0-3] |
PTM databases
GlyGeni | Q9BZJ0, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9BZJ0 |
PhosphoSitePlusi | Q9BZJ0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000101343, Expressed in calcaneal tendon and 228 other tissues |
ExpressionAtlasi | Q9BZJ0, baseline and differential |
Genevisiblei | Q9BZJ0, HS |
Organism-specific databases
HPAi | ENSG00000101343, Low tissue specificity |
Interactioni
Subunit structurei
Identified in the spliceosome C complex (PubMed:11991638, PubMed:28502770, PubMed:28076346). Present in a spliceosome complex assembled in vitro containing CRNKL1, HPRP8BP and SNRPB2 (PubMed:12084575). Isoform 2 seems to be predominant in the spliceosome complex (PubMed:12084575).
Interacts with PPIL2 (via the PPIase cyclophilin-type domain); they may form a trimeric complex with HSP90 (PubMed:15189447).
3 PublicationsBinary interactionsi
Q9BZJ0
With | #Exp. | IntAct |
---|---|---|
CCDC12 [Q8WUD4] | 2 | EBI-1049701,EBI-2557572 |
Protein-protein interaction databases
BioGRIDi | 119487, 112 interactors |
CORUMi | Q9BZJ0 |
IntActi | Q9BZJ0, 50 interactors |
MINTi | Q9BZJ0 |
STRINGi | 9606.ENSP00000366557 |
Miscellaneous databases
RNActi | Q9BZJ0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9BZJ0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 222 – 254 | HAT 1Add BLAST | 33 | |
Repeati | 256 – 288 | HAT 2Add BLAST | 33 | |
Repeati | 290 – 322 | HAT 3Add BLAST | 33 | |
Repeati | 324 – 355 | HAT 4Add BLAST | 32 | |
Repeati | 357 – 388 | HAT 5Add BLAST | 32 | |
Repeati | 390 – 425 | HAT 6Add BLAST | 36 | |
Repeati | 427 – 461 | HAT 7Add BLAST | 35 | |
Repeati | 471 – 503 | HAT 8Add BLAST | 33 | |
Repeati | 505 – 539 | HAT 9Add BLAST | 35 | |
Repeati | 549 – 585 | HAT 10Add BLAST | 37 | |
Repeati | 587 – 618 | HAT 11Add BLAST | 32 | |
Repeati | 620 – 652 | HAT 12Add BLAST | 33 | |
Repeati | 654 – 688 | HAT 13Add BLAST | 35 | |
Repeati | 690 – 721 | HAT 14Add BLAST | 32 | |
Repeati | 726 – 767 | HAT 15Add BLAST | 42 | |
Repeati | 769 – 807 | HAT 16Add BLAST | 39 | |
Repeati | 809 – 834 | HAT 17Add BLAST | 26 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 81 – 106 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 411 – 628 | Mediates interaction with HSP90By similarityAdd BLAST | 218 | |
Regioni | 827 – 848 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1915, Eukaryota |
GeneTreei | ENSGT00550000074931 |
HOGENOMi | CLU_2687132_0_0_1 |
InParanoidi | Q9BZJ0 |
OMAi | FTFSKIW |
PhylomeDBi | Q9BZJ0 |
TreeFami | TF300305 |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
InterProi | View protein in InterPro IPR003107, HAT IPR045075, Syf1-like IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR11246, PTHR11246, 1 hit |
Pfami | View protein in Pfam PF02184, HAT, 2 hits |
SMARTi | View protein in SMART SM00386, HAT, 13 hits |
SUPFAMi | SSF48452, SSF48452, 2 hits |
s (5+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ
60 70 80 90 100
FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHSTR CRKEDAQPGH
110 120 130 140 150
HGNGAASVTA QARGQRSVLQ VPLPVPRSCL FSESFVVSVS SQSRFLASVP
160 170 180 190 200
GTGVQRSTAA DMAASTAAGK QRIPKVAKVK NKAPAEVQIT AEQLLREAKE
210 220 230 240 250
RELELLPPPP QQKITDEEEL NDYKLRKRKT FEDNIRKNRT VISNWIKYAQ
260 270 280 290 300
WEESLKEIQR ARSIYERALD VDYRNITLWL KYAEMEMKNR QVNHARNIWD
310 320 330 340 350
RAITTLPRVN QFWYKYTYME EMLGNVAGAR QVFERWMEWQ PEEQAWHSYI
360 370 380 390 400
NFELRYKEVD RARTIYERFV LVHPDVKNWI KYARFEEKHA YFAHARKVYE
410 420 430 440 450
RAVEFFGDEH MDEHLYVAFA KFEENQKEFE RVRVIYKYAL DRISKQDAQE
460 470 480 490 500
LFKNYTIFEK KFGDRRGIED IIVSKRRFQY EEEVKANPHN YDAWFDYLRL
510 520 530 540 550
VESDAEAEAV REVYERAIAN VPPIQEKRHW KRYIYLWINY ALYEELEAKD
560 570 580 590 600
PERTRQVYQA SLELIPHKKF TFAKMWILYA QFEIRQKNLS LARRALGTSI
610 620 630 640 650
GKCPKNKLFK VYIELELQLR EFDRCRKLYE KFLEFGPENC TSWIKFAELE
660 670 680 690 700
TILGDIDRAR AIYELAISQP RLDMPEVLWK SYIDFEIEQE ETERTRNLYR
710 720 730 740 750
RLLQRTQHVK VWISFAQFEL SSGKEGSLTK CRQIYEEANK TMRNCEEKEE
760 770 780 790 800
RLMLLESWRS FEEEFGTASD KERVDKLMPE KVKKRRKVQT DDGSDAGWEE
810 820 830 840
YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES
The sequence of this isoform differs from the canonical sequence as follows:
23-34: Missing.
117-848: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
23-74: TSRLELRSYS...RSPCVAASQH → ILASLLVSTA...SRLKREVKGQ
75-848: Missing.
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5JY65 | Q5JY65_HUMAN | Crooked neck-like protein 1 | CRNKL1 | 836 | Annotation score: | ||
A0A0C4DGD7 | A0A0C4DGD7_HUMAN | Crooked neck-like protein 1 | CRNKL1 | 104 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 213 | K → R in AAF65571 (PubMed:11342225).Curated | 1 | |
Sequence conflicti | 213 | K → R in AAK01924 (PubMed:11342225).Curated | 1 | |
Sequence conflicti | 213 | K → R in AAK01925 (PubMed:11342225).Curated | 1 | |
Sequence conflicti | 513 | V → A in BAB14659 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_024995 | 35 | G → R1 PublicationCorresponds to variant dbSNP:rs7508949Ensembl. | 1 | |
Natural variantiVAR_049318 | 51 | F → L. Corresponds to variant dbSNP:rs2273058Ensembl. | 1 | |
Natural variantiVAR_049319 | 111 | Q → H. Corresponds to variant dbSNP:rs2255258Ensembl. | 1 | |
Natural variantiVAR_049320 | 158 | T → A. Corresponds to variant dbSNP:rs2255255Ensembl. | 1 | |
Natural variantiVAR_049321 | 843 | V → I. Corresponds to variant dbSNP:rs35201190Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002058 | 1 – 161 | Missing in isoform 2. 2 PublicationsAdd BLAST | 161 | |
Alternative sequenceiVSP_002060 | 23 – 74 | TSRLE…AASQH → ILASLLVSTALPTSSAAPGR RTPRAAARRTRSLVTMETVP PPSRLKREVKGQ in isoform 5. 1 PublicationAdd BLAST | 52 | |
Alternative sequenceiVSP_002059 | 23 – 34 | Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_002061 | 75 – 848 | Missing in isoform 5. 1 PublicationAdd BLAST | 774 | |
Alternative sequenceiVSP_002062 | 117 – 848 | Missing in isoform 4. 1 PublicationAdd BLAST | 732 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF255443 mRNA Translation: AAF65571.2 AF318302 mRNA Translation: AAK01924.1 AF318303 mRNA Translation: AAK01925.1 AF318304 mRNA Translation: AAK01926.1 AF318305 mRNA Translation: AAK01927.1 AF111802 mRNA Translation: AAL39004.1 AK023246 mRNA Translation: BAB14485.1 Different initiation. AK023728 mRNA Translation: BAB14659.1 AK292799 mRNA Translation: BAF85488.1 AL035454 Genomic DNA No translation available. AK022908 mRNA Translation: BAB14303.1 Different initiation. |
CCDSi | CCDS33446.1 [Q9BZJ0-1] CCDS63238.1 [Q9BZJ0-2] CCDS63239.1 [Q9BZJ0-3] |
RefSeqi | NP_001265554.1, NM_001278625.1 NP_001265555.1, NM_001278626.1 NP_001265556.1, NM_001278627.1 NP_001265557.1, NM_001278628.1 [Q9BZJ0-2] NP_057736.4, NM_016652.5 [Q9BZJ0-1] |
Genome annotation databases
Ensembli | ENST00000377340; ENSP00000366557; ENSG00000101343 ENST00000496549; ENSP00000428436; ENSG00000101343 [Q9BZJ0-5] ENST00000536226; ENSP00000440733; ENSG00000101343 [Q9BZJ0-2] |
GeneIDi | 51340 |
KEGGi | hsa:51340 |
MANE-Selecti | ENST00000536226.2; ENSP00000440733.1; NM_001278628.2; NP_001265557.1 [Q9BZJ0-2] |
UCSCi | uc002wrs.5, human [Q9BZJ0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF255443 mRNA Translation: AAF65571.2 AF318302 mRNA Translation: AAK01924.1 AF318303 mRNA Translation: AAK01925.1 AF318304 mRNA Translation: AAK01926.1 AF318305 mRNA Translation: AAK01927.1 AF111802 mRNA Translation: AAL39004.1 AK023246 mRNA Translation: BAB14485.1 Different initiation. AK023728 mRNA Translation: BAB14659.1 AK292799 mRNA Translation: BAF85488.1 AL035454 Genomic DNA No translation available. AK022908 mRNA Translation: BAB14303.1 Different initiation. |
CCDSi | CCDS33446.1 [Q9BZJ0-1] CCDS63238.1 [Q9BZJ0-2] CCDS63239.1 [Q9BZJ0-3] |
RefSeqi | NP_001265554.1, NM_001278625.1 NP_001265555.1, NM_001278626.1 NP_001265556.1, NM_001278627.1 NP_001265557.1, NM_001278628.1 [Q9BZJ0-2] NP_057736.4, NM_016652.5 [Q9BZJ0-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5MQF | electron microscopy | 5.90 | O | 1-848 | [»] | |
5XJC | electron microscopy | 3.60 | J | 1-848 | [»] | |
5YZG | electron microscopy | 4.10 | J | 1-848 | [»] | |
5Z56 | electron microscopy | 5.10 | J | 1-848 | [»] | |
5Z57 | electron microscopy | 6.50 | J | 1-848 | [»] | |
5Z58 | electron microscopy | 4.90 | J | 1-848 | [»] | |
6FF4 | electron microscopy | 16.00 | O | 1-848 | [»] | |
6FF7 | electron microscopy | 4.50 | O | 1-848 | [»] | |
6ICZ | electron microscopy | 3.00 | J | 1-848 | [»] | |
6ID0 | electron microscopy | 2.90 | J | 1-848 | [»] | |
6ID1 | electron microscopy | 2.86 | J | 1-848 | [»] | |
6QDV | electron microscopy | 3.30 | S | 1-848 | [»] | |
6ZYM | electron microscopy | 3.40 | O | 1-848 | [»] | |
7A5P | electron microscopy | 5.00 | O | 1-848 | [»] | |
7ABI | electron microscopy | 8.00 | O | 1-848 | [»] | |
7DVQ | electron microscopy | 2.89 | J | 1-848 | [»] | |
SMRi | Q9BZJ0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119487, 112 interactors |
CORUMi | Q9BZJ0 |
IntActi | Q9BZJ0, 50 interactors |
MINTi | Q9BZJ0 |
STRINGi | 9606.ENSP00000366557 |
PTM databases
GlyGeni | Q9BZJ0, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9BZJ0 |
PhosphoSitePlusi | Q9BZJ0 |
Genetic variation databases
BioMutai | CRNKL1 |
DMDMi | 147744555 |
Proteomic databases
EPDi | Q9BZJ0 |
jPOSTi | Q9BZJ0 |
MassIVEi | Q9BZJ0 |
MaxQBi | Q9BZJ0 |
PaxDbi | Q9BZJ0 |
PeptideAtlasi | Q9BZJ0 |
PRIDEi | Q9BZJ0 |
ProteomicsDBi | 79851 [Q9BZJ0-1] 79852 [Q9BZJ0-2] 79853 [Q9BZJ0-3] |
Protocols and materials databases
Antibodypediai | 9534, 64 antibodies from 13 providers |
DNASUi | 51340 |
Genome annotation databases
Ensembli | ENST00000377340; ENSP00000366557; ENSG00000101343 ENST00000496549; ENSP00000428436; ENSG00000101343 [Q9BZJ0-5] ENST00000536226; ENSP00000440733; ENSG00000101343 [Q9BZJ0-2] |
GeneIDi | 51340 |
KEGGi | hsa:51340 |
MANE-Selecti | ENST00000536226.2; ENSP00000440733.1; NM_001278628.2; NP_001265557.1 [Q9BZJ0-2] |
UCSCi | uc002wrs.5, human [Q9BZJ0-1] |
Organism-specific databases
CTDi | 51340 |
DisGeNETi | 51340 |
GeneCardsi | CRNKL1 |
HGNCi | HGNC:15762, CRNKL1 |
HPAi | ENSG00000101343, Low tissue specificity |
MIMi | 610952, gene |
neXtProti | NX_Q9BZJ0 |
OpenTargetsi | ENSG00000101343 |
PharmGKBi | PA26886 |
VEuPathDBi | HostDB:ENSG00000101343 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1915, Eukaryota |
GeneTreei | ENSGT00550000074931 |
HOGENOMi | CLU_2687132_0_0_1 |
InParanoidi | Q9BZJ0 |
OMAi | FTFSKIW |
PhylomeDBi | Q9BZJ0 |
TreeFami | TF300305 |
Enzyme and pathway databases
PathwayCommonsi | Q9BZJ0 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway |
SignaLinki | Q9BZJ0 |
Miscellaneous databases
BioGRID-ORCSi | 51340, 761 hits in 1051 CRISPR screens |
ChiTaRSi | CRNKL1, human |
GenomeRNAii | 51340 |
Pharosi | Q9BZJ0, Tbio |
PROi | PR:Q9BZJ0 |
RNActi | Q9BZJ0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000101343, Expressed in calcaneal tendon and 228 other tissues |
ExpressionAtlasi | Q9BZJ0, baseline and differential |
Genevisiblei | Q9BZJ0, HS |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
InterProi | View protein in InterPro IPR003107, HAT IPR045075, Syf1-like IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR11246, PTHR11246, 1 hit |
Pfami | View protein in Pfam PF02184, HAT, 2 hits |
SMARTi | View protein in SMART SM00386, HAT, 13 hits |
SUPFAMi | SSF48452, SSF48452, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CRNL1_HUMAN | |
Accessioni | Q9BZJ0Primary (citable) accession number: Q9BZJ0 Secondary accession number(s): A8K9T4 Q9NYD8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | May 15, 2007 | |
Last modified: | February 23, 2022 | |
This is version 177 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families