Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Oxysterol-binding protein-related protein 8

Gene

OSBPL8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:26206935). Binds oxysterol, 25-hydroxycholesterol and cholesterol (PubMed:17428193, PubMed:17991739, PubMed:21698267).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei485PhosphatidylserineBy similarity1
Binding sitei540PhosphatidylserineBy similarity1
Binding sitei706Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei710Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei714Phosphatidylinositol 4-phosphateBy similarity1

GO - Molecular functioni

  • cholesterol binding Source: BHF-UCL
  • lipid binding Source: GO_Central
  • phosphatidylinositol-4-phosphate binding Source: UniProtKB
  • phosphatidylserine binding Source: UniProtKB
  • phospholipid transporter activity Source: UniProtKB
  • sterol binding Source: GO_Central
  • sterol transporter activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processLipid transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-1482801 Acyl chain remodelling of PS

Protein family/group databases

TCDBi2.D.1.1.2 the pi4p/ps counter transporter (p/p-ct) family

Chemistry databases

SwissLipidsiSLP:000001535

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein-related protein 8
Short name:
ORP-8
Short name:
OSBP-related protein 8
Gene namesi
Name:OSBPL8
Synonyms:KIAA14511 Publication, ORP8, OSBP10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000091039.16
HGNCiHGNC:16396 OSBPL8
MIMi606736 gene
neXtProtiNX_Q9BZF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei871 – 888HelicalSequence analysisAdd BLAST18

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi514 – 515HH → AA: Impaired lipid countertransport between the endoplasmic reticulum and the plasma membrane. 1 Publication2

Organism-specific databases

DisGeNETi114882
OpenTargetsiENSG00000091039
PharmGKBiPA32832

Polymorphism and mutation databases

BioMutaiOSBPL8
DMDMi39932732

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001003781 – 889Oxysterol-binding protein-related protein 8Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei328PhosphoserineCombined sources1
Modified residuei342PhosphoserineCombined sources1
Modified residuei807PhosphoserineCombined sources1
Modified residuei808PhosphoserineCombined sources1
Modified residuei810PhosphoserineCombined sources1
Modified residuei814PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BZF1
MaxQBiQ9BZF1
PaxDbiQ9BZF1
PeptideAtlasiQ9BZF1
PRIDEiQ9BZF1
ProteomicsDBi79828
79829 [Q9BZF1-2]

PTM databases

iPTMnetiQ9BZF1
PhosphoSitePlusiQ9BZF1
SwissPalmiQ9BZF1

Miscellaneous databases

PMAP-CutDBiQ9BZF1

Expressioni

Tissue specificityi

Widely expressed (PubMed:11735225). Expressed at higher level in macrophages (PubMed:17991739).2 Publications

Gene expression databases

BgeeiENSG00000091039 Expressed in 249 organ(s), highest expression level in blood
CleanExiHS_OSBPL8
ExpressionAtlasiQ9BZF1 baseline and differential
GenevisibleiQ9BZF1 HS

Organism-specific databases

HPAiHPA001309

Interactioni

Subunit structurei

Interacts with SPAG5 (PubMed:24424245). Interacts with NUP62 (PubMed:21698267).2 Publications

Protein-protein interaction databases

BioGridi125383, 43 interactors
DIPiDIP-56130N
IntActiQ9BZF1, 17 interactors
STRINGi9606.ENSP00000261183

Structurei

Secondary structure

1889
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BZF1
SMRiQ9BZF1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BZF1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 265PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni420 – 425Phosphatidylinositol 4-phosphate bindingBy similarity6
Regioni420 – 425Phosphatidylserine bindingBy similarity6
Regioni482 – 485Phosphatidylinositol 4-phosphate bindingBy similarity4
Regioni514 – 515Phosphatidylinositol 4-phosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the OSBP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2210 Eukaryota
ENOG410XRW6 LUCA
GeneTreeiENSGT00550000074515
HOGENOMiHOG000233870
HOVERGENiHBG053375
InParanoidiQ9BZF1
KOiK22285
OMAiSYYHADI
OrthoDBiEOG091G0A3R
PhylomeDBiQ9BZF1
TreeFamiTF312807

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037239 OSBP_sf
IPR000648 Oxysterol-bd
IPR018494 Oxysterol-bd_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR10972 PTHR10972, 1 hit
PfamiView protein in Pfam
PF01237 Oxysterol_BP, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SUPFAMiSSF144000 SSF144000, 1 hit
PROSITEiView protein in PROSITE
PS01013 OSBP, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BZF1-1) [UniParc]FASTAAdd to basket
Also known as: ORP8L1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGGLADGEP DRTSLLGDSK DVLGPSTVVA NSDESQLLTP GKMSQRQGKE
60 70 80 90 100
AYPTPTKDLH QPSLSPASPH SQGFERGKED ISQNKDESSL SMSKSKSESK
110 120 130 140 150
LYNGSEKDSS TSSKLTKKES LKVQKKNYRE EKKRATKELL STITDPSVIV
160 170 180 190 200
MADWLKIRGT LKSWTKLWCV LKPGVLLIYK TQKNGQWVGT VLLNACEIIE
210 220 230 240 250
RPSKKDGFCF KLFHPLEQSI WAVKGPKGEA VGSITQPLPS SYLIIRATSE
260 270 280 290 300
SDGRCWMDAL ELALKCSSLL KRTMIREGKE HDLSVSSDST HVTFYGLLRA
310 320 330 340 350
NNLHSGDNFQ LNDSEIERQH FKDQDMYSDK SDKENDQEHD ESDNEVMGKS
360 370 380 390 400
EESDTDTSER QDDSYIEPEP VEPLKETTYT EQSHEELGEA GEASQTETVS
410 420 430 440 450
EENKSLIWTL LKQVRPGMDL SKVVLPTFIL EPRSFLDKLS DYYYHADFLS
460 470 480 490 500
EAALEENPYF RLKKVVKWYL SGFYKKPKGL KKPYNPILGE TFRCLWIHPR
510 520 530 540 550
TNSKTFYIAE QVSHHPPISA FYVSNRKDGF CLSGSILAKS KFYGNSLSAI
560 570 580 590 600
LEGEARLTFL NRGEDYVMTM PYAHCKGILY GTMTLELGGT VNITCQKTGY
610 620 630 640 650
SAILEFKLKP FLGSSDCVNQ ISGKLKLGKE VLATLEGHWD SEVFITDKKT
660 670 680 690 700
DNSEVFWNPT PDIKQWRLIR HTVKFEEQGD FESEKLWQRV TRAINAKDQT
710 720 730 740 750
EATQEKYVLE EAQRQAARDR KTKNEEWSCK LFELDPLTGE WHYKFADTRP
760 770 780 790 800
WDPLNDMIQF EKDGVIQTKV KHRTPMVSVP KMKHKPTRQQ KKVAKGYSSP
810 820 830 840 850
EPDIQDSSGS EAQSVKPSTR RKKGIELGDI QSSIESIKQT QEEIKRNIMA
860 870 880
LRNHLVSSTP ATDYFLQQKD YFIIFLLILL QVIINFMFK
Length:889
Mass (Da):101,196
Last modified:December 15, 2003 - v3
Checksum:i79DB8055BD15FE95
GO
Isoform 2 (identifier: Q9BZF1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-29: Missing.

Note: No experimental confirmation available.
Show »
Length:874
Mass (Da):99,714
Checksum:i309510B3F9E184DB
GO
Isoform 3 (identifier: Q9BZF1-3) [UniParc]FASTAAdd to basket
Also known as: ORP8S1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:847
Mass (Da):96,956
Checksum:i9DD6AB040C2FD20B
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VQX7F8VQX7_HUMAN
Oxysterol-binding protein
OSBPL8
721Annotation score:
F8VUA7F8VUA7_HUMAN
Oxysterol-binding protein
OSBPL8
694Annotation score:
F8VZ43F8VZ43_HUMAN
Oxysterol-binding protein-related p...
OSBPL8
157Annotation score:
F8VVE7F8VVE7_HUMAN
Oxysterol-binding protein-related p...
OSBPL8
111Annotation score:
F8VVD3F8VVD3_HUMAN
Oxysterol-binding protein-related p...
OSBPL8
76Annotation score:
F8VZB8F8VZB8_HUMAN
Oxysterol-binding protein-related p...
OSBPL8
58Annotation score:
F8W060F8W060_HUMAN
Oxysterol-binding protein-related p...
OSBPL8
52Annotation score:
F8W1F1F8W1F1_HUMAN
Oxysterol-binding protein-related p...
OSBPL8
28Annotation score:
F8VS33F8VS33_HUMAN
Oxysterol-binding protein-related p...
OSBPL8
41Annotation score:

Sequence cautioni

The sequence AAG53411 differs from that shown. Reason: Frameshift at position 30.Curated
The sequence BAA95975 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347M → V in CAH18345 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0458011 – 42Missing in isoform 3. CuratedAdd BLAST42
Alternative sequenceiVSP_00912015 – 29Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF392452 mRNA Translation: AAL40665.1
AB040884 mRNA Translation: BAA95975.3 Different initiation.
AK289997 mRNA Translation: BAF82686.1
CR749542 mRNA Translation: CAH18345.1
AC117491 Genomic DNA No translation available.
AC122687 Genomic DNA No translation available.
AC124943 Genomic DNA No translation available.
BC093834 mRNA Translation: AAH93834.1
BC101529 mRNA Translation: AAI01530.1
BC111728 mRNA Translation: AAI11729.1
AF323730 mRNA Translation: AAG53411.1 Frameshift.
CCDSiCCDS31862.1 [Q9BZF1-1]
CCDS41814.1 [Q9BZF1-3]
RefSeqiNP_001003712.1, NM_001003712.1 [Q9BZF1-3]
NP_001306581.1, NM_001319652.1 [Q9BZF1-3]
NP_001306584.1, NM_001319655.1
NP_065892.1, NM_020841.4 [Q9BZF1-1]
XP_005268678.1, XM_005268621.4 [Q9BZF1-1]
XP_006719287.1, XM_006719224.2 [Q9BZF1-3]
UniGeneiHs.430849

Genome annotation databases

EnsembliENST00000261183; ENSP00000261183; ENSG00000091039 [Q9BZF1-1]
ENST00000393249; ENSP00000376939; ENSG00000091039 [Q9BZF1-3]
ENST00000393250; ENSP00000376940; ENSG00000091039 [Q9BZF1-3]
ENST00000611266; ENSP00000478240; ENSG00000091039 [Q9BZF1-3]
GeneIDi114882
KEGGihsa:114882
UCSCiuc001sye.2 human [Q9BZF1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF392452 mRNA Translation: AAL40665.1
AB040884 mRNA Translation: BAA95975.3 Different initiation.
AK289997 mRNA Translation: BAF82686.1
CR749542 mRNA Translation: CAH18345.1
AC117491 Genomic DNA No translation available.
AC122687 Genomic DNA No translation available.
AC124943 Genomic DNA No translation available.
BC093834 mRNA Translation: AAH93834.1
BC101529 mRNA Translation: AAI01530.1
BC111728 mRNA Translation: AAI11729.1
AF323730 mRNA Translation: AAG53411.1 Frameshift.
CCDSiCCDS31862.1 [Q9BZF1-1]
CCDS41814.1 [Q9BZF1-3]
RefSeqiNP_001003712.1, NM_001003712.1 [Q9BZF1-3]
NP_001306581.1, NM_001319652.1 [Q9BZF1-3]
NP_001306584.1, NM_001319655.1
NP_065892.1, NM_020841.4 [Q9BZF1-1]
XP_005268678.1, XM_005268621.4 [Q9BZF1-1]
XP_006719287.1, XM_006719224.2 [Q9BZF1-3]
UniGeneiHs.430849

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V88NMR-A149-265[»]
5U77X-ray2.16A149-265[»]
5U78X-ray1.98A/B/C/D149-265[»]
ProteinModelPortaliQ9BZF1
SMRiQ9BZF1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125383, 43 interactors
DIPiDIP-56130N
IntActiQ9BZF1, 17 interactors
STRINGi9606.ENSP00000261183

Chemistry databases

SwissLipidsiSLP:000001535

Protein family/group databases

TCDBi2.D.1.1.2 the pi4p/ps counter transporter (p/p-ct) family

PTM databases

iPTMnetiQ9BZF1
PhosphoSitePlusiQ9BZF1
SwissPalmiQ9BZF1

Polymorphism and mutation databases

BioMutaiOSBPL8
DMDMi39932732

Proteomic databases

EPDiQ9BZF1
MaxQBiQ9BZF1
PaxDbiQ9BZF1
PeptideAtlasiQ9BZF1
PRIDEiQ9BZF1
ProteomicsDBi79828
79829 [Q9BZF1-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261183; ENSP00000261183; ENSG00000091039 [Q9BZF1-1]
ENST00000393249; ENSP00000376939; ENSG00000091039 [Q9BZF1-3]
ENST00000393250; ENSP00000376940; ENSG00000091039 [Q9BZF1-3]
ENST00000611266; ENSP00000478240; ENSG00000091039 [Q9BZF1-3]
GeneIDi114882
KEGGihsa:114882
UCSCiuc001sye.2 human [Q9BZF1-1]

Organism-specific databases

CTDi114882
DisGeNETi114882
EuPathDBiHostDB:ENSG00000091039.16
GeneCardsiOSBPL8
HGNCiHGNC:16396 OSBPL8
HPAiHPA001309
MIMi606736 gene
neXtProtiNX_Q9BZF1
OpenTargetsiENSG00000091039
PharmGKBiPA32832
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2210 Eukaryota
ENOG410XRW6 LUCA
GeneTreeiENSGT00550000074515
HOGENOMiHOG000233870
HOVERGENiHBG053375
InParanoidiQ9BZF1
KOiK22285
OMAiSYYHADI
OrthoDBiEOG091G0A3R
PhylomeDBiQ9BZF1
TreeFamiTF312807

Enzyme and pathway databases

ReactomeiR-HSA-1482801 Acyl chain remodelling of PS

Miscellaneous databases

ChiTaRSiOSBPL8 human
EvolutionaryTraceiQ9BZF1
GeneWikiiOSBPL8
GenomeRNAii114882
PMAP-CutDBiQ9BZF1
PROiPR:Q9BZF1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000091039 Expressed in 249 organ(s), highest expression level in blood
CleanExiHS_OSBPL8
ExpressionAtlasiQ9BZF1 baseline and differential
GenevisibleiQ9BZF1 HS

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR037239 OSBP_sf
IPR000648 Oxysterol-bd
IPR018494 Oxysterol-bd_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR10972 PTHR10972, 1 hit
PfamiView protein in Pfam
PF01237 Oxysterol_BP, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SUPFAMiSSF144000 SSF144000, 1 hit
PROSITEiView protein in PROSITE
PS01013 OSBP, 1 hit
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOSBL8_HUMAN
AccessioniPrimary (citable) accession number: Q9BZF1
Secondary accession number(s): A8K1T2
, E9PE66, E9PE68, Q52LQ3, Q68D75, Q8WXP8, Q9P277
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: December 15, 2003
Last modified: November 7, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again