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Protein

Kinetochore protein Nuf2

Gene

NUF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).8 Publications

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: GO_Central
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB
  • kinetochore organization Source: GO_Central
  • meiotic chromosome segregation Source: GO_Central
  • mitotic spindle organization Source: GO_Central

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SIGNORiQ9BZD4

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Nuf2
Short name:
hNuf2
Short name:
hNuf2R
Short name:
hsNuf2
Alternative name(s):
Cell division cycle-associated protein 1
Gene namesi
Name:NUF2
Synonyms:CDCA1, NUF2R
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143228.12
HGNCiHGNC:14621 NUF2
MIMi611772 gene
neXtProtiNX_Q9BZD4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi83540
OpenTargetsiENSG00000143228
PharmGKBiPA162398215

Polymorphism and mutation databases

BioMutaiNUF2
DMDMi115311829

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498131 – 464Kinetochore protein Nuf2Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei247PhosphoserineCombined sources1

Post-translational modificationi

Can be phosphorylated by AURKA and AURKB.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BZD4
MaxQBiQ9BZD4
PaxDbiQ9BZD4
PeptideAtlasiQ9BZD4
PRIDEiQ9BZD4
ProteomicsDBi79815

PTM databases

iPTMnetiQ9BZD4
PhosphoSitePlusiQ9BZD4

Expressioni

Gene expression databases

BgeeiENSG00000143228 Expressed in 126 organ(s), highest expression level in testis
CleanExiHS_NUF2
ExpressionAtlasiQ9BZD4 baseline and differential
GenevisibleiQ9BZD4 HS

Organism-specific databases

HPAiHPA059692

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B. Directly interacts with CENPE; this interaction determines CENPE kinetochore localization.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi123673, 79 interactors
ComplexPortaliCPX-550 Ndc80 complex
CORUMiQ9BZD4
DIPiDIP-36119N
IntActiQ9BZD4, 44 interactors
MINTiQ9BZD4
STRINGi9606.ENSP00000271452

Structurei

Secondary structure

1464
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BZD4
SMRiQ9BZD4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BZD4

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Interaction with the N-terminus of NDC80Add BLAST385
Regioni386 – 464Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili147 – 345Sequence analysisAdd BLAST199
Coiled coili389 – 459Sequence analysisAdd BLAST71

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4438 Eukaryota
ENOG410ZI4C LUCA
GeneTreeiENSGT00390000004199
HOGENOMiHOG000290701
HOVERGENiHBG082069
InParanoidiQ9BZD4
KOiK11548
OMAiREIVGCI
OrthoDBiEOG091G0YDU
PhylomeDBiQ9BZD4
TreeFamiTF101067

Family and domain databases

Gene3Di1.10.418.60, 1 hit
InterProiView protein in InterPro
IPR005549 Kinetochore_Nuf2
IPR038275 Nuf2_N_sf
PfamiView protein in Pfam
PF03800 Nuf2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q9BZD4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METLSFPRYN VAEIVIHIRN KILTGADGKN LTKNDLYPNP KPEVLHMIYM
60 70 80 90 100
RALQIVYGIR LEHFYMMPVN SEVMYPHLME GFLPFSNLVT HLDSFLPICR
110 120 130 140 150
VNDFETADIL CPKAKRTSRF LSGIINFIHF REACRETYME FLWQYKSSAD
160 170 180 190 200
KMQQLNAAHQ EALMKLERLD SVPVEEQEEF KQLSDGIQEL QQSLNQDFHQ
210 220 230 240 250
KTIVLQEGNS QKKSNISEKT KRLNELKLSV VSLKEIQESL KTKIVDSPEK
260 270 280 290 300
LKNYKEKMKD TVQKLKNARQ EVVEKYEIYG DSVDCLPSCQ LEVQLYQKKI
310 320 330 340 350
QDLSDNREKL ASILKESLNL EDQIESDESE LKKLKTEENS FKRLMIVKKE
360 370 380 390 400
KLATAQFKIN KKHEDVKQYK RTVIEDCNKV QEKRGAVYER VTTINQEIQK
410 420 430 440 450
IKLGIQQLKD AAEREKLKSQ EIFLNLKTAL EKYHDGIEKA AEDSYAKIDE
460
KTAELKRKMF KMST
Length:464
Mass (Da):54,304
Last modified:September 19, 2006 - v2
Checksum:i250BFED5AD87153B
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AQT4B1AQT4_HUMAN
Kinetochore protein Nuf2
NUF2
202Annotation score:
E9PQC4E9PQC4_HUMAN
Kinetochore protein Nuf2
NUF2
417Annotation score:
E9PKH1E9PKH1_HUMAN
Kinetochore protein Nuf2
NUF2
124Annotation score:
B1AQT3B1AQT3_HUMAN
Kinetochore protein Nuf2
NUF2
145Annotation score:
E9PP32E9PP32_HUMAN
Kinetochore protein Nuf2
NUF2
221Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027490229S → L2 PublicationsCorresponds to variant dbSNP:rs11802875Ensembl.1
Natural variantiVAR_027491239S → R. Corresponds to variant dbSNP:rs16852767Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050577 mRNA Translation: BAB59141.1
AB050578 mRNA Translation: BAB59142.1
AF326731 mRNA Translation: AAK01426.1
AK093348 mRNA Translation: BAC04140.1
AL592435 Genomic DNA No translation available.
BC008489 mRNA Translation: AAH08489.1
BC021171 mRNA Translation: AAH21171.2
CCDSiCCDS1245.1
RefSeqiNP_113611.2, NM_031423.3
NP_663735.2, NM_145697.2
UniGeneiHs.651950

Genome annotation databases

EnsembliENST00000271452; ENSP00000271452; ENSG00000143228
ENST00000367900; ENSP00000356875; ENSG00000143228
GeneIDi83540
KEGGihsa:83540
UCSCiuc001gcq.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050577 mRNA Translation: BAB59141.1
AB050578 mRNA Translation: BAB59142.1
AF326731 mRNA Translation: AAK01426.1
AK093348 mRNA Translation: BAC04140.1
AL592435 Genomic DNA No translation available.
BC008489 mRNA Translation: AAH08489.1
BC021171 mRNA Translation: AAH21171.2
CCDSiCCDS1245.1
RefSeqiNP_113611.2, NM_031423.3
NP_663735.2, NM_145697.2
UniGeneiHs.651950

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88C/D1-169[»]
3IZ0electron microscopy-D/F1-169[»]
ProteinModelPortaliQ9BZD4
SMRiQ9BZD4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123673, 79 interactors
ComplexPortaliCPX-550 Ndc80 complex
CORUMiQ9BZD4
DIPiDIP-36119N
IntActiQ9BZD4, 44 interactors
MINTiQ9BZD4
STRINGi9606.ENSP00000271452

PTM databases

iPTMnetiQ9BZD4
PhosphoSitePlusiQ9BZD4

Polymorphism and mutation databases

BioMutaiNUF2
DMDMi115311829

Proteomic databases

EPDiQ9BZD4
MaxQBiQ9BZD4
PaxDbiQ9BZD4
PeptideAtlasiQ9BZD4
PRIDEiQ9BZD4
ProteomicsDBi79815

Protocols and materials databases

DNASUi83540
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000271452; ENSP00000271452; ENSG00000143228
ENST00000367900; ENSP00000356875; ENSG00000143228
GeneIDi83540
KEGGihsa:83540
UCSCiuc001gcq.2 human

Organism-specific databases

CTDi83540
DisGeNETi83540
EuPathDBiHostDB:ENSG00000143228.12
GeneCardsiNUF2
HGNCiHGNC:14621 NUF2
HPAiHPA059692
MIMi611772 gene
neXtProtiNX_Q9BZD4
OpenTargetsiENSG00000143228
PharmGKBiPA162398215
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4438 Eukaryota
ENOG410ZI4C LUCA
GeneTreeiENSGT00390000004199
HOGENOMiHOG000290701
HOVERGENiHBG082069
InParanoidiQ9BZD4
KOiK11548
OMAiREIVGCI
OrthoDBiEOG091G0YDU
PhylomeDBiQ9BZD4
TreeFamiTF101067

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SIGNORiQ9BZD4

Miscellaneous databases

EvolutionaryTraceiQ9BZD4
GeneWikiiNUF2
GenomeRNAii83540
PROiPR:Q9BZD4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143228 Expressed in 126 organ(s), highest expression level in testis
CleanExiHS_NUF2
ExpressionAtlasiQ9BZD4 baseline and differential
GenevisibleiQ9BZD4 HS

Family and domain databases

Gene3Di1.10.418.60, 1 hit
InterProiView protein in InterPro
IPR005549 Kinetochore_Nuf2
IPR038275 Nuf2_N_sf
PfamiView protein in Pfam
PF03800 Nuf2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNUF2_HUMAN
AccessioniPrimary (citable) accession number: Q9BZD4
Secondary accession number(s): Q8WU69, Q96HJ4, Q96Q78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: November 7, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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