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Protein

Disintegrin and metalloproteinase domain-containing protein 33

Gene

ADAM33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi133Zinc; in inhibited formBy similarity1
Metal bindingi345Zinc; catalytic1 Publication1
Active sitei346PROSITE-ProRule annotation1
Metal bindingi349Zinc; catalytic1 Publication1
Metal bindingi355Zinc; catalytic1 Publication1

GO - Molecular functioni

  • metalloendopeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM12.244

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 33 (EC:3.4.24.-)
Short name:
ADAM 33
Gene namesi
Name:ADAM33
Synonyms:C20orf153
ORF Names:UNQ873/PRO1891
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000149451.17
HGNCiHGNC:15478 ADAM33
MIMi607114 gene
neXtProtiNX_Q9BZ11

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 701ExtracellularSequence analysisAdd BLAST672
Transmembranei702 – 722HelicalSequence analysisAdd BLAST21
Topological domaini723 – 813CytoplasmicSequence analysisAdd BLAST91

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Asthma (ASTHMA)3 Publications
Disease susceptibility is associated with variations affecting the gene represented in this entry.
Disease descriptionThe most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with wheezing due to spasmodic contraction of the bronchi.
See also OMIM:600807

Keywords - Diseasei

Asthma

Organism-specific databases

DisGeNETi80332
MIMi600807 phenotype
OpenTargetsiENSG00000149451
PharmGKBiPA24526

Chemistry databases

ChEMBLiCHEMBL6121
GuidetoPHARMACOLOGYi1673

Polymorphism and mutation databases

BioMutaiADAM33
DMDMi20137458

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002914230 – 203By similarityAdd BLAST174
ChainiPRO_0000029143204 – 813Disintegrin and metalloproteinase domain-containing protein 33Add BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi231N-linked (GlcNAc...) asparagine1
Glycosylationi276N-linked (GlcNAc...) asparagine1
Disulfide bondi320 ↔ 4041 Publication
Disulfide bondi360 ↔ 3881 Publication
Disulfide bondi361 ↔ 3711 Publication
Glycosylationi448N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi475 ↔ 495By similarity
Disulfide bondi653 ↔ 663By similarity
Disulfide bondi657 ↔ 669By similarity
Disulfide bondi671 ↔ 680By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9BZ11
PeptideAtlasiQ9BZ11
PRIDEiQ9BZ11
ProteomicsDBi79748
79749 [Q9BZ11-2]
79750 [Q9BZ11-3]

PTM databases

iPTMnetiQ9BZ11
PhosphoSitePlusiQ9BZ11

Expressioni

Tissue specificityi

Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.1 Publication

Gene expression databases

BgeeiENSG00000149451
CleanExiHS_ADAM33
ExpressionAtlasiQ9BZ11 baseline and differential
GenevisibleiQ9BZ11 HS

Organism-specific databases

HPAiHPA067152
HPA071758

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi123243, 57 interactors
IntActiQ9BZ11, 4 interactors
STRINGi9606.ENSP00000348912

Chemistry databases

BindingDBiQ9BZ11

Structurei

Secondary structure

1813
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi210 – 218Combined sources9
Helixi220 – 225Combined sources6
Turni226 – 228Combined sources3
Helixi230 – 248Combined sources19
Helixi249 – 251Combined sources3
Beta strandi253 – 262Combined sources10
Beta strandi264 – 266Combined sources3
Helixi275 – 292Combined sources18
Beta strandi296 – 303Combined sources8
Helixi307 – 309Combined sources3
Turni322 – 324Combined sources3
Beta strandi326 – 330Combined sources5
Beta strandi333 – 335Combined sources3
Helixi336 – 350Combined sources15
Helixi366 – 368Combined sources3
Helixi387 – 398Combined sources12
Turni399 – 402Combined sources4
Helixi403 – 406Combined sources4

3D structure databases

ProteinModelPortaliQ9BZ11
SMRiQ9BZ11
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BZ11

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini210 – 409Peptidase M12BPROSITE-ProRule annotationAdd BLAST200
Domaini417 – 503DisintegrinPROSITE-ProRule annotationAdd BLAST87
Domaini649 – 681EGF-likePROSITE-ProRule annotationAdd BLAST33

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi131 – 138Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi503 – 648Cys-richAdd BLAST146

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607 Eukaryota
ENOG410XX2M LUCA
GeneTreeiENSGT00910000144014
HOGENOMiHOG000230883
HOVERGENiHBG006978
InParanoidiQ9BZ11
KOiK08616
OMAiPDGCCVE
OrthoDBiEOG091G01NX
PhylomeDBiQ9BZ11
TreeFamiTF314733

Family and domain databases

CDDicd04269 ZnMc_adamalysin_II_like, 1 hit
Gene3Di3.40.390.10, 1 hit
4.10.70.10, 1 hit
InterProiView protein in InterPro
IPR006586 ADAM_Cys-rich
IPR018358 Disintegrin_CS
IPR001762 Disintegrin_dom
IPR036436 Disintegrin_dom_sf
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR034027 Reprolysin_adamalysin
PfamiView protein in Pfam
PF08516 ADAM_CR, 1 hit
PF00200 Disintegrin, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
PRINTSiPR00289 DISINTEGRIN
SMARTiView protein in SMART
SM00608 ACR, 1 hit
SM00050 DISIN, 1 hit
SUPFAMiSSF57552 SSF57552, 1 hit
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS00427 DISINTEGRIN_1, 1 hit
PS50214 DISINTEGRIN_2, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BZ11-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGWRPRRARG TPLLLLLLLL LLWPVPGAGV LQGHIPGQPV TPHWVLDGQP
60 70 80 90 100
WRTVSLEEPV SKPDMGLVAL EAEGQELLLE LEKNHRLLAP GYIETHYGPD
110 120 130 140 150
GQPVVLAPNH TDHCHYQGRV RGFPDSWVVL CTCSGMSGLI TLSRNASYYL
160 170 180 190 200
RPWPPRGSKD FSTHEIFRME QLLTWKGTCG HRDPGNKAGM TSLPGGPQSR
210 220 230 240 250
GRREARRTRK YLELYIVADH TLFLTRHRNL NHTKQRLLEV ANYVDQLLRT
260 270 280 290 300
LDIQVALTGL EVWTERDRSR VTQDANATLW AFLQWRRGLW AQRPHDSAQL
310 320 330 340 350
LTGRAFQGAT VGLAPVEGMC RAESSGGVST DHSELPIGAA ATMAHEIGHS
360 370 380 390 400
LGLSHDPDGC CVEAAAESGG CVMAAATGHP FPRVFSACSR RQLRAFFRKG
410 420 430 440 450
GGACLSNAPD PGLPVPPALC GNGFVEAGEE CDCGPGQECR DLCCFAHNCS
460 470 480 490 500
LRPGAQCAHG DCCVRCLLKP AGALCRQAMG DCDLPEFCTG TSSHCPPDVY
510 520 530 540 550
LLDGSPCARG SGYCWDGACP TLEQQCQQLW GPGSHPAPEA CFQVVNSAGD
560 570 580 590 600
AHGNCGQDSE GHFLPCAGRD ALCGKLQCQG GKPSLLAPHM VPVDSTVHLD
610 620 630 640 650
GQEVTCRGAL ALPSAQLDLL GLGLVEPGTQ CGPRMVCQSR RCRKNAFQEL
660 670 680 690 700
QRCLTACHSH GVCNSNHNCH CAPGWAPPFC DKPGFGGSMD SGPVQAENHD
710 720 730 740 750
TFLLAMLLSV LLPLLPGAGL AWCCYRLPGA HLQRCSWGCR RDPACSGPKD
760 770 780 790 800
GPHRDHPLGG VHPMELGPTA TGQPWPLDPE NSHEPSSHPE KPLPAVSPDP
810
QADQVQMPRS CLW
Length:813
Mass (Da):87,739
Last modified:March 27, 2002 - v2
Checksum:i90713A99668D5569
GO
Isoform 2 (identifier: Q9BZ11-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     636-661: Missing.

Show »
Length:787
Mass (Da):84,724
Checksum:iCBAF1B08B263A890
GO
Isoform 3 (identifier: Q9BZ11-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-478: Missing.
     636-661: Missing.

Note: No experimental confirmation available. By similarity with mouse isoform.
Show »
Length:309
Mass (Da):32,554
Checksum:iA60AC04E056CF264
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti802Missing in AAM80482 (PubMed:12110844).Curated1
Sequence conflicti802Missing in AAM80483 (PubMed:12110844).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030512109N → S1 PublicationCorresponds to variant dbSNP:rs41467948Ensembl.1
Natural variantiVAR_029143178T → A1 PublicationCorresponds to variant dbSNP:rs3918392Ensembl.1
Natural variantiVAR_030513272T → M1 PublicationCorresponds to variant dbSNP:rs41534847Ensembl.1
Natural variantiVAR_066337305A → V in a cutaneous metastatic melanoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_030514316V → I1 PublicationCorresponds to variant dbSNP:rs41459049Ensembl.1
Natural variantiVAR_030515336P → S1 PublicationCorresponds to variant dbSNP:rs41483049Ensembl.1
Natural variantiVAR_030516365A → S1 PublicationCorresponds to variant dbSNP:rs41419248Ensembl.1
Natural variantiVAR_030517441D → E1 PublicationCorresponds to variant dbSNP:rs41382144Ensembl.1
Natural variantiVAR_030518515W → R1 PublicationCorresponds to variant dbSNP:rs615436Ensembl.1
Natural variantiVAR_030519612L → H1 PublicationCorresponds to variant dbSNP:rs41453444Ensembl.1
Natural variantiVAR_030520710V → I1 PublicationCorresponds to variant dbSNP:rs3918396Ensembl.1
Natural variantiVAR_030521739C → G1 PublicationCorresponds to variant dbSNP:rs41434648Ensembl.1
Natural variantiVAR_030522742D → Y1 PublicationCorresponds to variant dbSNP:rs41462450Ensembl.1
Natural variantiVAR_021847764M → T1 PublicationCorresponds to variant dbSNP:rs2280091Ensembl.1
Natural variantiVAR_029144774P → S1 PublicationCorresponds to variant dbSNP:rs2280090Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0154211 – 478Missing in isoform 3. CuratedAdd BLAST478
Alternative sequenceiVSP_005495636 – 661Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055891 mRNA Translation: BAB83092.1
AF466287 mRNA Translation: AAM80482.1
AF466288 Genomic DNA Translation: AAM80483.1
AY358314 mRNA Translation: AAQ88680.1
DQ995342 Genomic DNA Translation: ABI97387.1
AL109804 Genomic DNA No translation available.
AL356755 Genomic DNA No translation available.
CCDSiCCDS13058.1 [Q9BZ11-1]
RefSeqiNP_001269376.1, NM_001282447.2
NP_079496.1, NM_025220.4 [Q9BZ11-1]
NP_694882.1, NM_153202.3 [Q9BZ11-2]
UniGeneiHs.173716

Genome annotation databases

EnsembliENST00000350009; ENSP00000322550; ENSG00000149451 [Q9BZ11-2]
ENST00000356518; ENSP00000348912; ENSG00000149451 [Q9BZ11-1]
GeneIDi80332
KEGGihsa:80332
UCSCiuc002wit.5 human [Q9BZ11-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiADA33_HUMAN
AccessioniPrimary (citable) accession number: Q9BZ11
Secondary accession number(s): A0A1K6
, Q5JT75, Q5JT76, Q8N0W6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: June 20, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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