Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroperoxide isomerase ALOXE3

Gene

ALOXE3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia.5 Publications

Catalytic activityi

A hydroperoxy icosatetraenoate = a hydroxy epoxy icosatrienoate.5 Publications
A hydroperoxy icosatetraenoate = an oxoicosatetraenoate + H2O.5 Publications

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Kineticsi

Has a 5 to 10 fold higher activity toward (12R)-HPETE (hydroperoxyeicosatetraenoic acid) compared to (12S)-HPETE and (15S)-HPETE. From (12R)-HPETE produces a stereoisomer of hepoxilin A3. More active on hydroperoxides with an R configuration than an S one.
  1. KM=46 µM for (12R)-HPETE3 Publications
  2. KM=28 µM for (12S)-HPETE3 Publications
  3. KM=32 µM for (15S)-HPETE3 Publications
  4. KM=50 µM for synthetic fatty acid 9E,11Z,14Z-20:3omega6 (at pH 7.5)3 Publications
  1. Vmax=3.256 µmol/min/mg enzyme for (12R)-HPETE (at pH 7.5)3 Publications
  2. Vmax=0.858 µmol/min/mg enzyme for (12S)-HPETE (at pH 7.5)3 Publications
  3. Vmax=0.484 µmol/min/mg enzyme for (15S)-HPETE (at pH 7.5)3 Publications

Pathwayi: hydroperoxy eicosatetraenoic acid biosynthesis

This protein is involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis and in Lipid metabolism.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi408Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi413Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi588Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi592Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi711Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Isomerase, Lyase, Oxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
UniPathwayi
UPA00222

UPA00881

Chemistry databases

SwissLipidsiSLP:000000656

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroperoxide isomerase ALOXE3 (EC:5.4.4.75 Publications)
Alternative name(s):
Epidermis-type lipoxygenase 31 Publication
Short name:
Epidermal LOX-3
Short name:
e-LOX-3
Short name:
eLOX-3
Hydroperoxy icosatetraenoate dehydratase (EC:4.2.1.1525 Publications)
Gene namesi
Name:ALOXE3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000179148.9
HGNCiHGNC:13743 ALOXE3
MIMi607206 gene
neXtProtiNX_Q9BYJ1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Ichthyosis, congenital, autosomal recessive 3 (ARCI3)5 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of autosomal recessive congenital ichthyosis, a disorder of keratinization with abnormal differentiation and desquamation of the epidermis, resulting in abnormal skin scaling over the whole body. The main skin phenotypes are lamellar ichthyosis (LI) and non-bullous congenital ichthyosiform erythroderma (NCIE), although phenotypic overlap within the same patient or among patients from the same family can occur. Lamellar ichthyosis is a condition often associated with an embedment in a collodion-like membrane at birth; skin scales later develop, covering the entire body surface. Non-bullous congenital ichthyosiform erythroderma characterized by fine whitish scaling on an erythrodermal background; larger brownish scales are present on the buttocks, neck and legs.
See also OMIM:606545
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069561237L → M in ARCI3; no effect on enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121434235EnsemblClinVar.1
Natural variantiVAR_069562281G → V in ARCI3; complete loss of the enzyme activity. 1 PublicationCorresponds to variant dbSNP:rs786205120Ensembl.1
Natural variantiVAR_069563344 – 347QYVA → P in ARCI3; complete loss of the enzyme activity. 1 Publication4
Natural variantiVAR_015175396R → S in ARCI3; complete loss of the enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121434234Ensembl.1
Natural variantiVAR_069564427L → P in ARCI3. 1 Publication1
Natural variantiVAR_015176500V → F in ARCI3; complete loss of the enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121434232Ensembl.1
Natural variantiVAR_069565630P → L in ARCI3; complete loss of the enzyme activity. 3 PublicationsCorresponds to variant dbSNP:rs147149459EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi451A → G: Increases the O2-dependent dioxygenase activity. 2 Publications1

Keywords - Diseasei

Disease mutation, Ichthyosis

Organism-specific databases

DisGeNETi59344
GeneReviewsiALOXE3
MalaCardsiALOXE3
MIMi606545 phenotype
OpenTargetsiENSG00000179148
Orphaneti79394 Congenital non-bullous ichthyosiform erythroderma
313 Lamellar ichthyosis
281122 Self-improving collodion baby
PharmGKBiPA24727

Polymorphism and mutation databases

BioMutaiALOXE3
DMDMi27923803

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002206911 – 711Hydroperoxide isomerase ALOXE3Add BLAST711

Proteomic databases

EPDiQ9BYJ1
PaxDbiQ9BYJ1
PeptideAtlasiQ9BYJ1
PRIDEiQ9BYJ1
ProteomicsDBi79655
79656 [Q9BYJ1-2]

PTM databases

iPTMnetiQ9BYJ1
PhosphoSitePlusiQ9BYJ1

Expressioni

Tissue specificityi

Predominantly expressed in skin.

Gene expression databases

BgeeiENSG00000179148 Expressed in 72 organ(s), highest expression level in skin of leg
CleanExiHS_ALOXE3
ExpressionAtlasiQ9BYJ1 baseline and differential
GenevisibleiQ9BYJ1 HS

Organism-specific databases

HPAiHPA023120
HPA078597

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ALOX12BO753422EBI-6925949,EBI-6925925

Protein-protein interaction databases

BioGridi121886, 29 interactors
IntActiQ9BYJ1, 3 interactors
STRINGi9606.ENSP00000314879

Structurei

3D structure databases

ProteinModelPortaliQ9BYJ1
SMRiQ9BYJ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 119PLATPROSITE-ProRule annotationAdd BLAST118
Domaini120 – 711LipoxygenasePROSITE-ProRule annotationAdd BLAST592

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
GeneTreeiENSGT00550000074415
HOGENOMiHOG000234358
HOVERGENiHBG005150
InParanoidiQ9BYJ1
KOiK18684
PhylomeDBiQ9BYJ1
TreeFamiTF105320

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BYJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAVYRLCVTT GPYLRAGTLD NISVTLVGTC GESPKQRLDR MGRDFAPGSV
60 70 80 90 100
QKYKVRCTAE LGELLLLRVH KERYAFFRKD SWYCSRICVT EPDGSVSHFP
110 120 130 140 150
CYQWIEGYCT VELRPGTART ICQDSLPLLL DHRTRELRAR QECYRWKIYA
160 170 180 190 200
PGFPCMVDVN SFQEMESDKK FALTKTTTCV DQGDSSGNRY LPGFPMKIDI
210 220 230 240 250
PSLMYMEPNV RYSATKTISL LFNAIPASLG MKLRGLLDRK GSWKKLDDMQ
260 270 280 290 300
NIFWCHKTFT TKYVTEHWCE DHFFGYQYLN GVNPVMLHCI SSLPSKLPVT
310 320 330 340 350
NDMVAPLLGQ DTCLQTELER GNIFLADYWI LAEAPTHCLN GRQQYVAAPL
360 370 380 390 400
CLLWLSPQGA LVPLAIQLSQ TPGPDSPIFL PTDSEWDWLL AKTWVRNSEF
410 420 430 440 450
LVHENNTHFL CTHLLCEAFA MATLRQLPLC HPIYKLLLPH TRYTLQVNTI
460 470 480 490 500
ARATLLNPEG LVDQVTSIGR QGLIYLMSTG LAHFTYTNFC LPDSLRARGV
510 520 530 540 550
LAIPNYHYRD DGLKIWAAIE SFVSEIVGYY YPSDASVQQD SELQAWTGEI
560 570 580 590 600
FAQAFLGRES SGFPSRLCTP GEMVKFLTAI IFNCSAQHAA VNSGQHDFGA
610 620 630 640 650
WMPNAPSSMR QPPPQTKGTT TLKTYLDTLP EVNISCNNLL LFWLVSQEPK
660 670 680 690 700
DQRPLGTYPD EHFTEEAPRR SIAAFQSRLA QISRDIQERN QGLALPYTYL
710
DPPLIENSVS I
Length:711
Mass (Da):80,543
Last modified:June 1, 2001 - v1
Checksum:iBDED1E4ED5CF6783
GO
Isoform 2 (identifier: Q9BYJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPRGAFRPCL...PGHPFLLPIM

Note: No experimental confirmation available.
Show »
Length:843
Mass (Da):95,186
Checksum:i75C69C336513F82C
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KPH2J3KPH2_HUMAN
Arachidonate lipoxygenase 3, isofor...
ALOXE3 hCG_31289
867Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti155C → R in CAC12843 (PubMed:11350124).Curated1
Sequence conflicti194F → L in AAG16899 (PubMed:12881489).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069561237L → M in ARCI3; no effect on enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121434235EnsemblClinVar.1
Natural variantiVAR_069562281G → V in ARCI3; complete loss of the enzyme activity. 1 PublicationCorresponds to variant dbSNP:rs786205120Ensembl.1
Natural variantiVAR_069563344 – 347QYVA → P in ARCI3; complete loss of the enzyme activity. 1 Publication4
Natural variantiVAR_015175396R → S in ARCI3; complete loss of the enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121434234Ensembl.1
Natural variantiVAR_069564427L → P in ARCI3. 1 Publication1
Natural variantiVAR_015176500V → F in ARCI3; complete loss of the enzyme activity. 2 PublicationsCorresponds to variant dbSNP:rs121434232Ensembl.1
Natural variantiVAR_069565630P → L in ARCI3; complete loss of the enzyme activity. 3 PublicationsCorresponds to variant dbSNP:rs147149459EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0432871M → MPRGAFRPCLPALYFAFLTC PTPEQRMSGTQAPDIHLGEP ARGTGCVRGKQTSIRVQDCG RREEARAASRELRREKAQEH PRESWAHPQPYPAPQPLALR PETQPCPACRSSPPGRLLLR PALPGHPFLLPIM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ269499 mRNA Translation: CAC12843.1
AJ305020
, AJ305021, AJ305023, AJ305025 Genomic DNA Translation: CAC34518.1
AF182218 mRNA Translation: AAG16899.1
AK296416 mRNA Translation: BAH12346.1
AK313677 mRNA Translation: BAG36428.1
CH471108 Genomic DNA Translation: EAW90094.1
BC101938 mRNA Translation: AAI01939.1
BC101939 mRNA Translation: AAI01940.1
BC103508 mRNA Translation: AAI03509.1
BC104724 mRNA Translation: AAI04725.1
CCDSiCCDS11130.1 [Q9BYJ1-1]
CCDS54084.1 [Q9BYJ1-2]
RefSeqiNP_001159432.1, NM_001165960.1 [Q9BYJ1-2]
NP_067641.2, NM_021628.2 [Q9BYJ1-1]
UniGeneiHs.232770

Genome annotation databases

EnsembliENST00000318227; ENSP00000314879; ENSG00000179148 [Q9BYJ1-2]
ENST00000448843; ENSP00000400581; ENSG00000179148 [Q9BYJ1-1]
GeneIDi59344
KEGGihsa:59344
UCSCiuc010cnr.4 human [Q9BYJ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

about water - Issue 153 of September 2013

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ269499 mRNA Translation: CAC12843.1
AJ305020
, AJ305021, AJ305023, AJ305025 Genomic DNA Translation: CAC34518.1
AF182218 mRNA Translation: AAG16899.1
AK296416 mRNA Translation: BAH12346.1
AK313677 mRNA Translation: BAG36428.1
CH471108 Genomic DNA Translation: EAW90094.1
BC101938 mRNA Translation: AAI01939.1
BC101939 mRNA Translation: AAI01940.1
BC103508 mRNA Translation: AAI03509.1
BC104724 mRNA Translation: AAI04725.1
CCDSiCCDS11130.1 [Q9BYJ1-1]
CCDS54084.1 [Q9BYJ1-2]
RefSeqiNP_001159432.1, NM_001165960.1 [Q9BYJ1-2]
NP_067641.2, NM_021628.2 [Q9BYJ1-1]
UniGeneiHs.232770

3D structure databases

ProteinModelPortaliQ9BYJ1
SMRiQ9BYJ1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121886, 29 interactors
IntActiQ9BYJ1, 3 interactors
STRINGi9606.ENSP00000314879

Chemistry databases

SwissLipidsiSLP:000000656

PTM databases

iPTMnetiQ9BYJ1
PhosphoSitePlusiQ9BYJ1

Polymorphism and mutation databases

BioMutaiALOXE3
DMDMi27923803

Proteomic databases

EPDiQ9BYJ1
PaxDbiQ9BYJ1
PeptideAtlasiQ9BYJ1
PRIDEiQ9BYJ1
ProteomicsDBi79655
79656 [Q9BYJ1-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318227; ENSP00000314879; ENSG00000179148 [Q9BYJ1-2]
ENST00000448843; ENSP00000400581; ENSG00000179148 [Q9BYJ1-1]
GeneIDi59344
KEGGihsa:59344
UCSCiuc010cnr.4 human [Q9BYJ1-1]

Organism-specific databases

CTDi59344
DisGeNETi59344
EuPathDBiHostDB:ENSG00000179148.9
GeneCardsiALOXE3
GeneReviewsiALOXE3
HGNCiHGNC:13743 ALOXE3
HPAiHPA023120
HPA078597
MalaCardsiALOXE3
MIMi606545 phenotype
607206 gene
neXtProtiNX_Q9BYJ1
OpenTargetsiENSG00000179148
Orphaneti79394 Congenital non-bullous ichthyosiform erythroderma
313 Lamellar ichthyosis
281122 Self-improving collodion baby
PharmGKBiPA24727
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
GeneTreeiENSGT00550000074415
HOGENOMiHOG000234358
HOVERGENiHBG005150
InParanoidiQ9BYJ1
KOiK18684
PhylomeDBiQ9BYJ1
TreeFamiTF105320

Enzyme and pathway databases

UniPathwayi
UPA00222

UPA00881

ReactomeiR-HSA-2142712 Synthesis of 12-eicosatetraenoic acid derivatives

Miscellaneous databases

ChiTaRSiALOXE3 human
GeneWikiiALOXE3
GenomeRNAii59344
PROiPR:Q9BYJ1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000179148 Expressed in 72 organ(s), highest expression level in skin of leg
CleanExiHS_ALOXE3
ExpressionAtlasiQ9BYJ1 baseline and differential
GenevisibleiQ9BYJ1 HS

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLOXE3_HUMAN
AccessioniPrimary (citable) accession number: Q9BYJ1
Secondary accession number(s): B2R981
, B7Z3W0, Q3ZB74, Q9H4F2, Q9HC22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  7. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again