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Protein

MKI67 FHA domain-interacting nucleolar phosphoprotein

Gene

NIFK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • RNA binding Source: UniProtKB

GO - Biological processi

  • protein-containing complex assembly Source: UniProtKB
  • rRNA metabolic process Source: UniProtKB
  • rRNA transcription Source: UniProtKB

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MKI67 FHA domain-interacting nucleolar phosphoprotein
Alternative name(s):
Nucleolar phosphoprotein Nopp34
Nucleolar protein interacting with the FHA domain of pKI-67
Short name:
hNIFK
Gene namesi
Name:NIFK
Synonyms:MKI67IP, NOPP34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000155438.11
HGNCiHGNC:17838 NIFK
MIMi611970 gene
neXtProtiNX_Q9BYG3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi230S → A: Loss of phosphorylation site. 1 Publication1
Mutagenesisi234T → A: Loss of phosphorylation site. Abrogates interaction with MKI67. 2 Publications1
Mutagenesisi235P → A: Reduces phosphorylation at T-234. 1 Publication1
Mutagenesisi238T → A: Loss of phosphorylation site. Abrogates interaction with MKI67. 2 Publications1
Mutagenesisi239P → A: Reduces phosphorylation at T-234 and T-238. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000155438
PharmGKBiPA38470

Polymorphism and mutation databases

BioMutaiMKI67IP
DMDMi71151919

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000816292 – 293MKI67 FHA domain-interacting nucleolar phosphoproteinAdd BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Cross-linki38Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei114Omega-N-methylated arginine; by PRMT1 and PRMT81 Publication1
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei145PhosphoserineCombined sources1
Cross-linki179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki192Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei218PhosphoserineCombined sources1
Modified residuei223PhosphothreonineCombined sources1
Modified residuei230Phosphoserine1 Publication1
Modified residuei234PhosphothreonineCombined sources2 Publications1
Modified residuei238PhosphothreonineCombined sources1 Publication1
Modified residuei244Omega-N-methylated arginine; by PRMT1 and PRMT81 Publication1
Modified residuei245Omega-N-methylated arginine; by PRMT1 and PRMT81 Publication1
Modified residuei247PhosphoserineCombined sources1
Cross-linki271Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki271Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei279PhosphothreonineCombined sources1
Modified residuei284Omega-N-methylated arginine; by PRMT1 and PRMT81 Publication1

Post-translational modificationi

Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.3 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9BYG3
MaxQBiQ9BYG3
PaxDbiQ9BYG3
PeptideAtlasiQ9BYG3
PRIDEiQ9BYG3
ProteomicsDBi79637

2D gel databases

SWISS-2DPAGEiQ9BYG3

PTM databases

iPTMnetiQ9BYG3
PhosphoSitePlusiQ9BYG3

Expressioni

Gene expression databases

BgeeiENSG00000155438
CleanExiHS_MKI67IP
ExpressionAtlasiQ9BYG3 baseline and differential
GenevisibleiQ9BYG3 HS

Organism-specific databases

HPAiCAB015382
CAB075728
CAB075729
HPA035735
HPA035736

Interactioni

Subunit structurei

Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi124066, 151 interactors
DIPiDIP-28134N
IntActiQ9BYG3, 17 interactors
MINTiQ9BYG3
STRINGi9606.ENSP00000285814

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi239 – 251Combined sources13
Helixi254 – 257Combined sources4
Beta strandi260 – 265Combined sources6

3D structure databases

ProteinModelPortaliQ9BYG3
SMRiQ9BYG3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BYG3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 123RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni226 – 269Interaction with MKI67Add BLAST44

Phylogenomic databases

eggNOGiKOG4208 Eukaryota
ENOG4111NF9 LUCA
GeneTreeiENSGT00390000011515
HOGENOMiHOG000242795
HOVERGENiHBG057796
InParanoidiQ9BYG3
KOiK14838
OMAiDPTPVCT
OrthoDBiEOG091G0RMN
PhylomeDBiQ9BYG3
TreeFamiTF315137

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR021043 NIFK_FHA_Ki67_binding
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF12196 hNIFK_binding, 1 hit
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BYG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATFSGPAGP ILSLNPQEDV EFQKEVAQVR KRITQRKKQE QLTPGVVYVR
60 70 80 90 100
HLPNLLDETQ IFSYFSQFGT VTRFRLSRSK RTGNSKGYAF VEFESEDVAK
110 120 130 140 150
IVAETMNNYL FGERLLECHF MPPEKVHKEL FKDWNIPFKQ PSYPSVKRYN
160 170 180 190 200
RNRTLTQKLR MEERFKKKER LLRKKLAKKG IDYDFPSLIL QKTESISKTN
210 220 230 240 250
RQTSTKGQVL RKKKKKVSGT LDTPEKTVDS QGPTPVCTPT FLERRKSQVA
260 270 280 290
ELNDDDKDDE IVFKQPISCV KEEIQETQTP THSRKKRRRS SNQ
Length:293
Mass (Da):34,222
Last modified:June 1, 2001 - v1
Checksum:i9D288ECF2B4CE119
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027182144P → Q1 PublicationCorresponds to variant dbSNP:rs17852212Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044971 mRNA Translation: BAB41210.1
AK291903 mRNA Translation: BAF84592.1
AC018737 Genomic DNA Translation: AAY14830.1
CH471103 Genomic DNA Translation: EAW95261.1
BC012457 mRNA Translation: AAH12457.1
BC022990 mRNA Translation: AAH22990.1
BC024238 mRNA Translation: AAH24238.1
CCDSiCCDS2135.1
RefSeqiNP_115766.3, NM_032390.4
UniGeneiHs.367842

Genome annotation databases

EnsembliENST00000285814; ENSP00000285814; ENSG00000155438
GeneIDi84365
KEGGihsa:84365
UCSCiuc002tnk.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMK67I_HUMAN
AccessioniPrimary (citable) accession number: Q9BYG3
Secondary accession number(s): A8K788, Q8TB66, Q96ED4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: July 18, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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