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UniProtKB - Q9BYG3 (MK67I_HUMAN)
Protein
MKI67 FHA domain-interacting nucleolar phosphoprotein
Gene
NIFK
Organism
Homo sapiens (Human)
Status
Functioni
GO - Molecular functioni
- RNA binding Source: UniProtKB
GO - Biological processi
- maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- protein-containing complex assembly Source: UniProtKB
- rRNA metabolic process Source: UniProtKB
- rRNA transcription Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9BYG3 |
SignaLinki | Q9BYG3 |
SIGNORi | Q9BYG3 |
Names & Taxonomyi
Protein namesi | Recommended name: MKI67 FHA domain-interacting nucleolar phosphoproteinAlternative name(s): Nucleolar phosphoprotein Nopp34 Nucleolar protein interacting with the FHA domain of pKI-67 Short name: hNIFK |
Gene namesi | Name:NIFK Synonyms:MKI67IP, NOPP34 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17838, NIFK |
MIMi | 611970, gene |
neXtProti | NX_Q9BYG3 |
VEuPathDBi | HostDB:ENSG00000155438 |
Subcellular locationi
Nucleus
Other locations
Note: Localizes to mitotic chromosomes in conjunction with MKI67.
Nucleus
- condensed nuclear chromosome Source: UniProtKB
- nucleolus Source: UniProtKB
- nucleoplasm Source: UniProtKB
Other locations
- chromosome Source: HPA
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 230 | S → A: Loss of phosphorylation site. 1 Publication | 1 | |
Mutagenesisi | 234 | T → A: Loss of phosphorylation site. Abrogates interaction with MKI67. 2 Publications | 1 | |
Mutagenesisi | 235 | P → A: Reduces phosphorylation at T-234. 1 Publication | 1 | |
Mutagenesisi | 238 | T → A: Loss of phosphorylation site. Abrogates interaction with MKI67. 2 Publications | 1 | |
Mutagenesisi | 239 | P → A: Reduces phosphorylation at T-234 and T-238. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 84365 |
OpenTargetsi | ENSG00000155438 |
PharmGKBi | PA38470 |
Miscellaneous databases
Pharosi | Q9BYG3, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4523443 |
Genetic variation databases
BioMutai | NIFK |
DMDMi | 71151919 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000081629 | 2 – 293 | MKI67 FHA domain-interacting nucleolar phosphoproteinAdd BLAST | 292 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Cross-linki | 38 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 114 | Omega-N-methylarginine; by PRMT1 and PRMT81 Publication | 1 | |
Cross-linki | 139 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 145 | PhosphoserineCombined sources | 1 | |
Cross-linki | 179 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 192 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 218 | PhosphoserineCombined sources | 1 | |
Modified residuei | 223 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 230 | Phosphoserine1 Publication | 1 | |
Modified residuei | 234 | PhosphothreonineCombined sources2 Publications | 1 | |
Modified residuei | 238 | PhosphothreonineCombined sources1 Publication | 1 | |
Modified residuei | 244 | Omega-N-methylated arginine; by PRMT1 and PRMT81 Publication | 1 | |
Modified residuei | 245 | Omega-N-methylated arginine; by PRMT1 and PRMT81 Publication | 1 | |
Modified residuei | 247 | PhosphoserineCombined sources | 1 | |
Cross-linki | 271 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources | ||
Cross-linki | 271 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 279 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 284 | Omega-N-methylarginine; by PRMT1 and PRMT81 Publication | 1 |
Post-translational modificationi
Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.3 Publications
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9BYG3 |
jPOSTi | Q9BYG3 |
MassIVEi | Q9BYG3 |
MaxQBi | Q9BYG3 |
PaxDbi | Q9BYG3 |
PeptideAtlasi | Q9BYG3 |
PRIDEi | Q9BYG3 |
ProteomicsDBi | 79637 |
2D gel databases
SWISS-2DPAGEi | Q9BYG3 |
PTM databases
GlyGeni | Q9BYG3, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9BYG3 |
PhosphoSitePlusi | Q9BYG3 |
Expressioni
Gene expression databases
Bgeei | ENSG00000155438, Expressed in calcaneal tendon and 217 other tissues |
ExpressionAtlasi | Q9BYG3, baseline and differential |
Genevisiblei | Q9BYG3, HS |
Organism-specific databases
HPAi | ENSG00000155438, Low tissue specificity |
Interactioni
Subunit structurei
Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.
1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 124066, 376 interactors |
DIPi | DIP-28134N |
IntActi | Q9BYG3, 87 interactors |
MINTi | Q9BYG3 |
STRINGi | 9606.ENSP00000285814 |
Miscellaneous databases
RNActi | Q9BYG3, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q9BYG3 |
SMRi | Q9BYG3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9BYG3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 123 | RRMPROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 197 – 239 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 226 – 269 | Interaction with MKI67Add BLAST | 44 | |
Regioni | 271 – 293 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 223 – 237 | Polar residuesSequence analysisAdd BLAST | 15 |
Phylogenomic databases
eggNOGi | KOG4208, Eukaryota |
GeneTreei | ENSGT00390000011515 |
HOGENOMi | CLU_025741_1_0_1 |
InParanoidi | Q9BYG3 |
OMAi | FREWHVP |
OrthoDBi | 1513142at2759 |
PhylomeDBi | Q9BYG3 |
TreeFami | TF315137 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR021043, NIFK_FHA_Ki67-binding IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12196, hNIFK_binding, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9BYG3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATFSGPAGP ILSLNPQEDV EFQKEVAQVR KRITQRKKQE QLTPGVVYVR
60 70 80 90 100
HLPNLLDETQ IFSYFSQFGT VTRFRLSRSK RTGNSKGYAF VEFESEDVAK
110 120 130 140 150
IVAETMNNYL FGERLLECHF MPPEKVHKEL FKDWNIPFKQ PSYPSVKRYN
160 170 180 190 200
RNRTLTQKLR MEERFKKKER LLRKKLAKKG IDYDFPSLIL QKTESISKTN
210 220 230 240 250
RQTSTKGQVL RKKKKKVSGT LDTPEKTVDS QGPTPVCTPT FLERRKSQVA
260 270 280 290
ELNDDDKDDE IVFKQPISCV KEEIQETQTP THSRKKRRRS SNQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J808 | C9J808_HUMAN | MKI67 FHA domain-interacting nucleo... | NIFK | 164 | Annotation score: | ||
C9J6C5 | C9J6C5_HUMAN | MKI67 FHA domain-interacting nucleo... | NIFK | 166 | Annotation score: | ||
H7BZL0 | H7BZL0_HUMAN | MKI67 FHA domain-interacting nucleo... | NIFK | 102 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027182 | 144 | P → Q1 PublicationCorresponds to variant dbSNP:rs17852212Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB044971 mRNA Translation: BAB41210.1 AK291903 mRNA Translation: BAF84592.1 AC018737 Genomic DNA Translation: AAY14830.1 CH471103 Genomic DNA Translation: EAW95261.1 BC012457 mRNA Translation: AAH12457.1 BC022990 mRNA Translation: AAH22990.1 BC024238 mRNA Translation: AAH24238.1 |
CCDSi | CCDS2135.1 |
RefSeqi | NP_115766.3, NM_032390.4 |
Genome annotation databases
Ensembli | ENST00000285814; ENSP00000285814; ENSG00000155438 |
GeneIDi | 84365 |
KEGGi | hsa:84365 |
MANE-Selecti | ENST00000285814.9; ENSP00000285814.4; NM_032390.5; NP_115766.3 |
UCSCi | uc002tnk.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB044971 mRNA Translation: BAB41210.1 AK291903 mRNA Translation: BAF84592.1 AC018737 Genomic DNA Translation: AAY14830.1 CH471103 Genomic DNA Translation: EAW95261.1 BC012457 mRNA Translation: AAH12457.1 BC022990 mRNA Translation: AAH22990.1 BC024238 mRNA Translation: AAH24238.1 |
CCDSi | CCDS2135.1 |
RefSeqi | NP_115766.3, NM_032390.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AFF | NMR | - | B | 226-269 | [»] | |
BMRBi | Q9BYG3 | |||||
SMRi | Q9BYG3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124066, 376 interactors |
DIPi | DIP-28134N |
IntActi | Q9BYG3, 87 interactors |
MINTi | Q9BYG3 |
STRINGi | 9606.ENSP00000285814 |
Chemistry databases
ChEMBLi | CHEMBL4523443 |
PTM databases
GlyGeni | Q9BYG3, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9BYG3 |
PhosphoSitePlusi | Q9BYG3 |
Genetic variation databases
BioMutai | NIFK |
DMDMi | 71151919 |
2D gel databases
SWISS-2DPAGEi | Q9BYG3 |
Proteomic databases
EPDi | Q9BYG3 |
jPOSTi | Q9BYG3 |
MassIVEi | Q9BYG3 |
MaxQBi | Q9BYG3 |
PaxDbi | Q9BYG3 |
PeptideAtlasi | Q9BYG3 |
PRIDEi | Q9BYG3 |
ProteomicsDBi | 79637 |
Protocols and materials databases
Antibodypediai | 4205, 402 antibodies from 34 providers |
DNASUi | 84365 |
Genome annotation databases
Ensembli | ENST00000285814; ENSP00000285814; ENSG00000155438 |
GeneIDi | 84365 |
KEGGi | hsa:84365 |
MANE-Selecti | ENST00000285814.9; ENSP00000285814.4; NM_032390.5; NP_115766.3 |
UCSCi | uc002tnk.4, human |
Organism-specific databases
CTDi | 84365 |
DisGeNETi | 84365 |
GeneCardsi | NIFK |
HGNCi | HGNC:17838, NIFK |
HPAi | ENSG00000155438, Low tissue specificity |
MIMi | 611970, gene |
neXtProti | NX_Q9BYG3 |
OpenTargetsi | ENSG00000155438 |
PharmGKBi | PA38470 |
VEuPathDBi | HostDB:ENSG00000155438 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4208, Eukaryota |
GeneTreei | ENSGT00390000011515 |
HOGENOMi | CLU_025741_1_0_1 |
InParanoidi | Q9BYG3 |
OMAi | FREWHVP |
OrthoDBi | 1513142at2759 |
PhylomeDBi | Q9BYG3 |
TreeFami | TF315137 |
Enzyme and pathway databases
PathwayCommonsi | Q9BYG3 |
SignaLinki | Q9BYG3 |
SIGNORi | Q9BYG3 |
Miscellaneous databases
BioGRID-ORCSi | 84365, 760 hits in 993 CRISPR screens |
ChiTaRSi | NIFK, human |
EvolutionaryTracei | Q9BYG3 |
GeneWikii | MKI67IP |
GenomeRNAii | 84365 |
Pharosi | Q9BYG3, Tbio |
PROi | PR:Q9BYG3 |
RNActi | Q9BYG3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000155438, Expressed in calcaneal tendon and 217 other tissues |
ExpressionAtlasi | Q9BYG3, baseline and differential |
Genevisiblei | Q9BYG3, HS |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR021043, NIFK_FHA_Ki67-binding IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12196, hNIFK_binding, 1 hit PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MK67I_HUMAN | |
Accessioni | Q9BYG3Primary (citable) accession number: Q9BYG3 Secondary accession number(s): A8K788, Q8TB66, Q96ED4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references