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Protein

Dual specificity protein phosphatase 16

Gene

DUSP16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei244Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • MAP kinase tyrosine/serine/threonine phosphatase activity Source: GO_Central
  • phosphatase activity Source: UniProtKB
  • phosphoprotein phosphatase activity Source: UniProtKB
  • protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
  • protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-HSA-112409 RAF-independent MAPK1/3 activation
R-HSA-5675221 Negative regulation of MAPK pathway
SignaLinkiQ9BY84
SIGNORiQ9BY84

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 16 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Mitogen-activated protein kinase phosphatase 7
Short name:
MAP kinase phosphatase 7
Short name:
MKP-7
Gene namesi
Name:DUSP16
Synonyms:KIAA1700, MKP7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111266.8
HGNCiHGNC:17909 DUSP16
MIMi607175 gene
neXtProtiNX_Q9BY84

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi80824
OpenTargetsiENSG00000111266
PharmGKBiPA38475

Polymorphism and mutation databases

BioMutaiDUSP16
DMDMi20137933

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948261 – 665Dual specificity protein phosphatase 16Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55(Microbial infection) N6-acetyllysine; by EIS1 Publication1
Modified residuei446Phosphoserine; by MAPK11 Publication1
Modified residuei501PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity.1 Publication
(Microbial infection) Acetylated at Lys-55 by the M.tuberculosis Eis protein; this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BY84
MaxQBiQ9BY84
PaxDbiQ9BY84
PeptideAtlasiQ9BY84
PRIDEiQ9BY84
ProteomicsDBi79602

PTM databases

DEPODiQ9BY84
iPTMnetiQ9BY84
PhosphoSitePlusiQ9BY84

Expressioni

Gene expression databases

BgeeiENSG00000111266 Expressed in 193 organ(s), highest expression level in adrenal tissue
CleanExiHS_DUSP16
ExpressionAtlasiQ9BY84 baseline and differential
GenevisibleiQ9BY84 HS

Organism-specific databases

HPAiHPA020326

Interactioni

Subunit structurei

Interacts with ARRB2.1 Publication

Protein-protein interaction databases

BioGridi123321, 22 interactors
IntActiQ9BY84, 28 interactors
STRINGi9606.ENSP00000228862

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BY84
SMRiQ9BY84
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BY84

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 137RhodanesePROSITE-ProRule annotationAdd BLAST116
Domaini227 – 289Tyrosine-protein phosphataseAdd BLAST63

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOGENOMiHOG000082452
HOVERGENiHBG005541
InParanoidiQ9BY84
KOiK04459
OMAiSWYFAAE
OrthoDBiEOG091G0249
PhylomeDBiQ9BY84
TreeFamiTF105122

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.40.250.10, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR008343 MKP
IPR029021 Prot-tyrosine_phosphatase-like
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF00581 Rhodanese, 1 hit
PRINTSiPR01764 MAPKPHPHTASE
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SM00450 RHOD, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BY84-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAHEMIGTQI VTERLVALLE SGTEKVLLID SRPFVEYNTS HILEAININC
60 70 80 90 100
SKLMKRRLQQ DKVLITELIQ HSAKHKVDID CSQKVVVYDQ SSQDVASLSS
110 120 130 140 150
DCFLTVLLGK LEKSFNSVHL LAGGFAEFSR CFPGLCEGKS TLVPTCISQP
160 170 180 190 200
CLPVANIGPT RILPNLYLGC QRDVLNKELM QQNGIGYVLN ASNTCPKPDF
210 220 230 240 250
IPESHFLRVP VNDSFCEKIL PWLDKSVDFI EKAKASNGCV LVHCLAGISR
260 270 280 290 300
SATIAIAYIM KRMDMSLDEA YRFVKEKRPT ISPNFNFLGQ LLDYEKKIKN
310 320 330 340 350
QTGASGPKSK LKLLHLEKPN EPVPAVSEGG QKSETPLSPP CADSATSEAA
360 370 380 390 400
GQRPVHPASV PSVPSVQPSL LEDSPLVQAL SGLHLSADRL EDSNKLKRSF
410 420 430 440 450
SLDIKSVSYS ASMAASLHGF SSSEDALEYY KPSTTLDGTN KLCQFSPVQE
460 470 480 490 500
LSEQTPETSP DKEEASIPKK LQTARPSDSQ SKRLHSVRTS SSGTAQRSLL
510 520 530 540 550
SPLHRSGSVE DNYHTSFLFG LSTSQQHLTK SAGLGLKGWH SDILAPQTST
560 570 580 590 600
PSLTSSWYFA TESSHFYSAS AIYGGSASYS AYSCSQLPTC GDQVYSVRRR
610 620 630 640 650
QKPSDRADSR RSWHEESPFE KQFKRRSCQM EFGESIMSEN RSREELGKVG
660
SQSSFSGSME IIEVS
Length:665
Mass (Da):73,102
Last modified:June 1, 2001 - v1
Checksum:i1BD853FF08460DFF
GO
Isoform 2 (identifier: Q9BY84-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-143: GFAEFSRCFPGLCEGKSTLV → ADAAEWDWLCVKCQQYLSKA
     144-665: Missing.

Note: No experimental confirmation available.
Show »
Length:143
Mass (Da):16,078
Checksum:i5213A213AA7E5974
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5H5X4F5H5X4_HUMAN
Dual-specificity protein phosphatas...
DUSP16
25Annotation score:

Sequence cautioni

The sequence BAB21791 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05175323T → M. Corresponds to variant dbSNP:rs36049447Ensembl.1
Natural variantiVAR_051754366V → M. Corresponds to variant dbSNP:rs3809199Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056981124 – 143GFAEF…KSTLV → ADAAEWDWLCVKCQQYLSKA in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_056982144 – 665Missing in isoform 2. 1 PublicationAdd BLAST522

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052156 mRNA Translation: BAB40814.1
AF506796 mRNA Translation: AAN75120.1
AY038927 mRNA Translation: AAK69770.1
AB051487 mRNA Translation: BAB21791.1 Different initiation.
AC007619 Genomic DNA No translation available.
AC092824 Genomic DNA No translation available.
BC109235 mRNA Translation: AAI09236.1
CCDSiCCDS8650.1 [Q9BY84-1]
RefSeqiNP_085143.1, NM_030640.2 [Q9BY84-1]
XP_006719218.1, XM_006719155.2 [Q9BY84-1]
XP_011519158.1, XM_011520856.1 [Q9BY84-1]
XP_011519159.1, XM_011520857.1
UniGeneiHs.536535

Genome annotation databases

EnsembliENST00000228862; ENSP00000228862; ENSG00000111266 [Q9BY84-2]
ENST00000298573; ENSP00000298573; ENSG00000111266 [Q9BY84-1]
ENST00000626413; ENSP00000487512; ENSG00000280962 [Q9BY84-1]
ENST00000628303; ENSP00000487034; ENSG00000280962 [Q9BY84-2]
GeneIDi80824
KEGGihsa:80824
UCSCiuc001rao.3 human [Q9BY84-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052156 mRNA Translation: BAB40814.1
AF506796 mRNA Translation: AAN75120.1
AY038927 mRNA Translation: AAK69770.1
AB051487 mRNA Translation: BAB21791.1 Different initiation.
AC007619 Genomic DNA No translation available.
AC092824 Genomic DNA No translation available.
BC109235 mRNA Translation: AAI09236.1
CCDSiCCDS8650.1 [Q9BY84-1]
RefSeqiNP_085143.1, NM_030640.2 [Q9BY84-1]
XP_006719218.1, XM_006719155.2 [Q9BY84-1]
XP_011519158.1, XM_011520856.1 [Q9BY84-1]
XP_011519159.1, XM_011520857.1
UniGeneiHs.536535

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VSWX-ray2.20A/B5-150[»]
3TG3X-ray2.68A/B/C/D5-138[»]
4YR8X-ray2.40B/D/G/H156-301[»]
ProteinModelPortaliQ9BY84
SMRiQ9BY84
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123321, 22 interactors
IntActiQ9BY84, 28 interactors
STRINGi9606.ENSP00000228862

PTM databases

DEPODiQ9BY84
iPTMnetiQ9BY84
PhosphoSitePlusiQ9BY84

Polymorphism and mutation databases

BioMutaiDUSP16
DMDMi20137933

Proteomic databases

EPDiQ9BY84
MaxQBiQ9BY84
PaxDbiQ9BY84
PeptideAtlasiQ9BY84
PRIDEiQ9BY84
ProteomicsDBi79602

Protocols and materials databases

DNASUi80824
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228862; ENSP00000228862; ENSG00000111266 [Q9BY84-2]
ENST00000298573; ENSP00000298573; ENSG00000111266 [Q9BY84-1]
ENST00000626413; ENSP00000487512; ENSG00000280962 [Q9BY84-1]
ENST00000628303; ENSP00000487034; ENSG00000280962 [Q9BY84-2]
GeneIDi80824
KEGGihsa:80824
UCSCiuc001rao.3 human [Q9BY84-1]

Organism-specific databases

CTDi80824
DisGeNETi80824
EuPathDBiHostDB:ENSG00000111266.8
GeneCardsiDUSP16
HGNCiHGNC:17909 DUSP16
HPAiHPA020326
MIMi607175 gene
neXtProtiNX_Q9BY84
OpenTargetsiENSG00000111266
PharmGKBiPA38475
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118902
HOGENOMiHOG000082452
HOVERGENiHBG005541
InParanoidiQ9BY84
KOiK04459
OMAiSWYFAAE
OrthoDBiEOG091G0249
PhylomeDBiQ9BY84
TreeFamiTF105122

Enzyme and pathway databases

ReactomeiR-HSA-112409 RAF-independent MAPK1/3 activation
R-HSA-5675221 Negative regulation of MAPK pathway
SignaLinkiQ9BY84
SIGNORiQ9BY84

Miscellaneous databases

ChiTaRSiDUSP16 human
EvolutionaryTraceiQ9BY84
GeneWikiiDUSP16
GenomeRNAii80824
PROiPR:Q9BY84
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111266 Expressed in 193 organ(s), highest expression level in adrenal tissue
CleanExiHS_DUSP16
ExpressionAtlasiQ9BY84 baseline and differential
GenevisibleiQ9BY84 HS

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.40.250.10, 1 hit
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR008343 MKP
IPR029021 Prot-tyrosine_phosphatase-like
IPR001763 Rhodanese-like_dom
IPR036873 Rhodanese-like_dom_sf
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF00581 Rhodanese, 1 hit
PRINTSiPR01764 MAPKPHPHTASE
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SM00450 RHOD, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
SSF52821 SSF52821, 1 hit
PROSITEiView protein in PROSITE
PS50206 RHODANESE_3, 1 hit
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDUS16_HUMAN
AccessioniPrimary (citable) accession number: Q9BY84
Secondary accession number(s): Q547C7, Q96QS2, Q9C0G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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