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Protein

Eukaryotic translation initiation factor 2A

Gene

EIF2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.1 Publication

Caution

Was initially thought to constitute the ortholog of prokaryotic IF-2 (infB) protein.Curated

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • ribosome binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB
  • tRNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

SIGNORiQ9BY44

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2A
Short name:
eIF-2A
Alternative name(s):
65 kDa eukaryotic translation initiation factor 2A
Cleaved into the following chain:
Gene namesi
Name:EIF2A
ORF Names:CDA02, MSTP004, MSTP089
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000144895.11
HGNCiHGNC:3254 EIF2A
MIMi609234 gene
neXtProtiNX_Q9BY44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi83939
OpenTargetsiENSG00000144895
PharmGKBiPA143485453

Polymorphism and mutation databases

BioMutaiEIF2A
DMDMi209572747

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004244661 – 585Eukaryotic translation initiation factor 2AAdd BLAST585
Initiator methionineiRemoved; alternate1 Publication
ChainiPRO_00002860762 – 585Eukaryotic translation initiation factor 2A, N-terminally processedAdd BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei2N-acetylalanine; in Eukaryotic translation initiation factor 2A, N-terminally processed1 Publication1
Modified residuei5PhosphothreonineCombined sources1
Modified residuei503PhosphoserineCombined sources1
Modified residuei506PhosphoserineCombined sources1
Modified residuei517PhosphoserineCombined sources1
Modified residuei518PhosphothreonineCombined sources1
Modified residuei526PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BY44
MaxQBiQ9BY44
PaxDbiQ9BY44
PeptideAtlasiQ9BY44
PRIDEiQ9BY44
ProteomicsDBi79581
79582 [Q9BY44-2]

PTM databases

iPTMnetiQ9BY44
PhosphoSitePlusiQ9BY44
SwissPalmiQ9BY44

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.1 Publication

Gene expression databases

BgeeiENSG00000144895 Expressed in 200 organ(s), highest expression level in secondary oocyte
CleanExiHS_EIF2A
ExpressionAtlasiQ9BY44 baseline and differential
GenevisibleiQ9BY44 HS

Organism-specific databases

HPAiHPA036256

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123822, 52 interactors
DIPiDIP-40272N
ELMiQ9BY44
IntActiQ9BY44, 11 interactors
MINTiQ9BY44
STRINGi9606.ENSP00000417229

Structurei

3D structure databases

ProteinModelPortaliQ9BY44
SMRiQ9BY44
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati56 – 100WD 1Add BLAST45
Repeati101 – 159WD 2Add BLAST59
Repeati160 – 210WD 3Add BLAST51
Repeati211 – 264WD 4Add BLAST54
Repeati265 – 306WD 5Add BLAST42
Repeati307 – 348WD 6Add BLAST42
Repeati349 – 391WD 7Add BLAST43

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili531 – 582Sequence analysisAdd BLAST52

Sequence similaritiesi

Belongs to the WD repeat EIF2A family.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2315 Eukaryota
COG5354 LUCA
GeneTreeiENSGT00730000111053
HOGENOMiHOG000193775
HOVERGENiHBG107842
InParanoidiQ9BY44
KOiK15026
OMAiQKSQTGW
OrthoDBiEOG091G04PE
PhylomeDBiQ9BY44
TreeFamiTF105866

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR011387 TIF2A
IPR013979 TIF_beta_prop-like
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR13227 PTHR13227, 1 hit
PfamiView protein in Pfam
PF08662 eIF2A, 1 hit
PIRSFiPIRSF017222 eIF2A, 1 hit
SUPFAMiSSF50978 SSF50978, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BY44-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPSTPLLTV RGSEGLYMVN GPPHFTESTV FPRESGKNCK VCIFSKDGTL
60 70 80 90 100
FAWGNGEKVN IISVTNKGLL HSFDLLKAVC LEFSPKNTVL ATWQPYTTSK
110 120 130 140 150
DGTAGIPNLQ LYDVKTGTCL KSFIQKKMQN WCPSWSEDET LCARNVNNEV
160 170 180 190 200
HFFENNNFNT IANKLHLQKI NDFVLSPGPQ PYKVAVYVPG SKGAPSFVRL
210 220 230 240 250
YQYPNFAGPH AALANKSFFK ADKVTMLWNK KATAVLVIAS TDVDKTGASY
260 270 280 290 300
YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT
310 320 330 340 350
IFNLKCDPVF DFGTGPRNAA YYSPHGHILV LAGFGNLRGQ MEVWDVKNYK
360 370 380 390 400
LISKPVASDS TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSILHKY
410 420 430 440 450
DVPSNAELWQ VSWQPFLDGI FPAKTITYQA VPSEVPNEEP KVATAYRPPA
460 470 480 490 500
LRNKPITNSK LHEEEPPQNM KPQSGNDKPL SKTALKNQRK HEAKKAAKQE
510 520 530 540 550
ARSDKSPDLA PTPAPQSTPR NTVSQSISGD PEIDKKIKNL KKKLKAIEQL
560 570 580
KEQAATGKQL EKNQLEKIQK ETALLQELED LELGI
Length:585
Mass (Da):64,990
Last modified:October 14, 2008 - v3
Checksum:i63D63C0676F232C9
GO
Isoform 2 (identifier: Q9BY44-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-213: Missing.
     214-219: ANKSFF → MFQEV

Note: No experimental confirmation available.
Show »
Length:371
Mass (Da):41,339
Checksum:i0D4454F18732DBD2
GO
Isoform 3 (identifier: Q9BY44-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     34-58: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):62,289
Checksum:iAE5E408990B0CC21
GO
Isoform 4 (identifier: Q9BY44-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-158: Missing.

Note: No experimental confirmation available.
Show »
Length:524
Mass (Da):57,994
Checksum:iE6F35813714656EF
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WAE5F8WAE5_HUMAN
Eukaryotic translation initiation f...
EIF2A
580Annotation score:
H7C5R5H7C5R5_HUMAN
Eukaryotic translation initiation f...
EIF2A
316Annotation score:
H7C5Q3H7C5Q3_HUMAN
Eukaryotic translation initiation f...
EIF2A
158Annotation score:
C9IZE1C9IZE1_HUMAN
Eukaryotic translation initiation f...
EIF2A
236Annotation score:
F8WAT3F8WAT3_HUMAN
Eukaryotic translation initiation f...
EIF2A
75Annotation score:
F8WF18F8WF18_HUMAN
Eukaryotic translation initiation f...
EIF2A
59Annotation score:
H7C5Q4H7C5Q4_HUMAN
Eukaryotic translation initiation f...
EIF2A
40Annotation score:

Sequence cautioni

The sequence AAK14926 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAM83402 differs from that shown. Reason: Frameshift at positions 82 and 90.Curated
The sequence AAQ13506 differs from that shown. Reason: Frameshift at position 513.Curated
The sequence AAQ13612 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7L → F in AAQ13612 (Ref. 4) Curated1
Sequence conflicti14 – 16EGL → LRT in AAQ13612 (Ref. 4) Curated3
Sequence conflicti54G → A in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti55N → I in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti84S → P in BAB55058 (PubMed:14702039).Curated1
Sequence conflicti150V → A in AAQ13612 (Ref. 4) Curated1
Sequence conflicti173F → S in AAQ13612 (Ref. 4) Curated1
Sequence conflicti261T → P in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti263G → F in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti268V → L in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti292Y → S in AAM83402 (PubMed:12133843).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03206697T → S5 PublicationsCorresponds to variant dbSNP:rs1132979Ensembl.1
Natural variantiVAR_032067582E → K1 PublicationCorresponds to variant dbSNP:rs17850813Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0249751 – 213Missing in isoform 2. 1 PublicationAdd BLAST213
Alternative sequenceiVSP_05604734 – 58Missing in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_05604898 – 158Missing in isoform 4. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_024976214 – 219ANKSFF → MFQEV in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497978 mRNA Translation: AAM83402.1 Frameshift.
AK027356 mRNA Translation: BAB55058.1
AK298586 mRNA Translation: BAG60776.1
AK293993 mRNA Translation: BAG57357.1
AF212241 mRNA Translation: AAK14926.1 Different initiation.
AF109358 mRNA Translation: AAQ13506.1 Frameshift.
AF172818 mRNA Translation: AAQ13612.1 Different initiation.
AC107426 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW78830.1
CH471052 Genomic DNA Translation: EAW78831.1
BC011885 mRNA Translation: AAH11885.1
CCDSiCCDS46935.1 [Q9BY44-1]
CCDS82859.1 [Q9BY44-4]
CCDS82861.1 [Q9BY44-3]
RefSeqiNP_001305972.1, NM_001319043.1 [Q9BY44-3]
NP_001305973.1, NM_001319044.1 [Q9BY44-4]
NP_001305974.1, NM_001319045.1
NP_001305975.1, NM_001319046.1 [Q9BY44-2]
NP_114414.2, NM_032025.4 [Q9BY44-1]
UniGeneiHs.655782

Genome annotation databases

EnsembliENST00000406576; ENSP00000385292; ENSG00000144895 [Q9BY44-4]
ENST00000460851; ENSP00000417229; ENSG00000144895 [Q9BY44-1]
ENST00000487799; ENSP00000420537; ENSG00000144895 [Q9BY44-3]
GeneIDi83939
KEGGihsa:83939
UCSCiuc003eya.4 human [Q9BY44-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497978 mRNA Translation: AAM83402.1 Frameshift.
AK027356 mRNA Translation: BAB55058.1
AK298586 mRNA Translation: BAG60776.1
AK293993 mRNA Translation: BAG57357.1
AF212241 mRNA Translation: AAK14926.1 Different initiation.
AF109358 mRNA Translation: AAQ13506.1 Frameshift.
AF172818 mRNA Translation: AAQ13612.1 Different initiation.
AC107426 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW78830.1
CH471052 Genomic DNA Translation: EAW78831.1
BC011885 mRNA Translation: AAH11885.1
CCDSiCCDS46935.1 [Q9BY44-1]
CCDS82859.1 [Q9BY44-4]
CCDS82861.1 [Q9BY44-3]
RefSeqiNP_001305972.1, NM_001319043.1 [Q9BY44-3]
NP_001305973.1, NM_001319044.1 [Q9BY44-4]
NP_001305974.1, NM_001319045.1
NP_001305975.1, NM_001319046.1 [Q9BY44-2]
NP_114414.2, NM_032025.4 [Q9BY44-1]
UniGeneiHs.655782

3D structure databases

ProteinModelPortaliQ9BY44
SMRiQ9BY44
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123822, 52 interactors
DIPiDIP-40272N
ELMiQ9BY44
IntActiQ9BY44, 11 interactors
MINTiQ9BY44
STRINGi9606.ENSP00000417229

PTM databases

iPTMnetiQ9BY44
PhosphoSitePlusiQ9BY44
SwissPalmiQ9BY44

Polymorphism and mutation databases

BioMutaiEIF2A
DMDMi209572747

Proteomic databases

EPDiQ9BY44
MaxQBiQ9BY44
PaxDbiQ9BY44
PeptideAtlasiQ9BY44
PRIDEiQ9BY44
ProteomicsDBi79581
79582 [Q9BY44-2]

Protocols and materials databases

DNASUi83939
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000406576; ENSP00000385292; ENSG00000144895 [Q9BY44-4]
ENST00000460851; ENSP00000417229; ENSG00000144895 [Q9BY44-1]
ENST00000487799; ENSP00000420537; ENSG00000144895 [Q9BY44-3]
GeneIDi83939
KEGGihsa:83939
UCSCiuc003eya.4 human [Q9BY44-1]

Organism-specific databases

CTDi83939
DisGeNETi83939
EuPathDBiHostDB:ENSG00000144895.11
GeneCardsiEIF2A
H-InvDBiHIX0200477
HGNCiHGNC:3254 EIF2A
HPAiHPA036256
MIMi609234 gene
neXtProtiNX_Q9BY44
OpenTargetsiENSG00000144895
PharmGKBiPA143485453
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2315 Eukaryota
COG5354 LUCA
GeneTreeiENSGT00730000111053
HOGENOMiHOG000193775
HOVERGENiHBG107842
InParanoidiQ9BY44
KOiK15026
OMAiQKSQTGW
OrthoDBiEOG091G04PE
PhylomeDBiQ9BY44
TreeFamiTF105866

Enzyme and pathway databases

SIGNORiQ9BY44

Miscellaneous databases

GeneWikiiEIF2A
GenomeRNAii83939
PROiPR:Q9BY44
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144895 Expressed in 200 organ(s), highest expression level in secondary oocyte
CleanExiHS_EIF2A
ExpressionAtlasiQ9BY44 baseline and differential
GenevisibleiQ9BY44 HS

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR011387 TIF2A
IPR013979 TIF_beta_prop-like
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR13227 PTHR13227, 1 hit
PfamiView protein in Pfam
PF08662 eIF2A, 1 hit
PIRSFiPIRSF017222 eIF2A, 1 hit
SUPFAMiSSF50978 SSF50978, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF2A_HUMAN
AccessioniPrimary (citable) accession number: Q9BY44
Secondary accession number(s): A8MPS6
, B4DF96, B4DQ14, D3DNI9, Q5QTR2, Q7Z4E9, Q8NFM1, Q96EW9, Q96K81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 14, 2008
Last modified: November 7, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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