UniProtKB - Q9BY43 (CHM4A_HUMAN)
Charged multivesicular body protein 4a
CHMP4A
Functioni
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- lipid binding Source: UniProtKB-KW
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- endosomal transport Source: Reactome
- late endosome to vacuole transport via multivesicular body sorting pathway Source: GO_Central
- macroautophagy Source: ParkinsonsUK-UCL
- membrane invagination Source: UniProtKB
- midbody abscission Source: UniProtKB
- mitotic metaphase plate congression Source: UniProtKB
- multivesicular body assembly Source: ParkinsonsUK-UCL
- negative regulation of autophagosome assembly Source: UniProtKB
- negative regulation of neuron death Source: UniProtKB
- nucleus organization Source: UniProtKB
- plasma membrane tubulation Source: UniProtKB
- posttranslational protein targeting to endoplasmic reticulum membrane Source: UniProtKB
- protein polymerization Source: UniProtKB
- vesicle budding from membrane Source: UniProtKB
- viral budding via host ESCRT complex Source: UniProtKB
- viral life cycle Source: Reactome
Keywordsi
Biological process | Protein transport, Transport |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9BY43 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-1632852, Macroautophagy R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy R-HSA-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III R-HSA-9679504, Translation of Replicase and Assembly of the Replication Transcription Complex |
SignaLinki | Q9BY43 |
Names & Taxonomyi
Protein namesi | Recommended name: Charged multivesicular body protein 4aAlternative name(s): Chromatin-modifying protein 4a Short name: CHMP4a SNF7 homolog associated with Alix-2 SNF7-1 Short name: hSnf-1 Vacuolar protein sorting-associated protein 32-1 Short name: Vps32-1 Short name: hVps32-1 |
Gene namesi | Name:CHMP4A Synonyms:C14orf123, SHAX2 ORF Names:CDA04, HSPC134 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000254505.9 |
HGNCi | HGNC:20274, CHMP4A |
MIMi | 610051, gene |
neXtProti | NX_Q9BY43 |
Subcellular locationi
Endosome
- Late endosome membrane Curated; Peripheral membrane protein Curated
Other locations
Note: Membrane-associated. Localizes to large vesicle-like structures. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
Cytosol
- cytosol Source: Reactome
Endosome
- ESCRT III complex Source: UniProtKB
- late endosome membrane Source: UniProtKB-SubCell
- multivesicular body Source: GO_Central
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- cytoplasmic side of plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- membrane coat Source: UniProtKB
- midbody Source: FlyBase
Keywords - Cellular componenti
Cytoplasmic vesicle, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 182 – 222 | Missing : Membrane association; releases autoinhibition. 1 PublicationAdd BLAST | 41 | |
Mutagenesisi | 209 | E → A: Reduces interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 214 | L → A: Abolishes interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 217 | L → A: Abolishes interaction with PDCD6IP. 1 Publication | 1 | |
Mutagenesisi | 220 | W → A: Abolishes interaction with PDCD6IP. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 29082 |
OpenTargetsi | ENSG00000254505 |
PharmGKBi | PA134888743 |
Miscellaneous databases
Pharosi | Q9BY43, Tbio |
Polymorphism and mutation databases
BioMutai | CHMP4A |
DMDMi | 90152096 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211488 | 1 – 222 | Charged multivesicular body protein 4aAdd BLAST | 222 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 196 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9BY43 |
jPOSTi | Q9BY43 |
MassIVEi | Q9BY43 |
MaxQBi | Q9BY43 |
PaxDbi | Q9BY43 |
PeptideAtlasi | Q9BY43 |
PRIDEi | Q9BY43 |
ProteomicsDBi | 60344 79580 [Q9BY43-1] |
PTM databases
iPTMneti | Q9BY43 |
PhosphoSitePlusi | Q9BY43 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000254505, Expressed in frontal cortex and 116 other tissues |
ExpressionAtlasi | Q9BY43, baseline and differential |
Genevisiblei | Q9BY43, HS |
Organism-specific databases
HPAi | ENSG00000254505, Low tissue specificity |
Interactioni
Subunit structurei
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. Self-associates; overexpression leads to the assembly of filaments that curve and associate to create circular rings.
Interacts with CHMP2A.
Interacts with CHMP3; the interaction requires the release of CHMP4A autoinhibition.
Interacts with CHMP4B.
Interacts with CHMP4C.
Interacts with CHMP6.
Interacts with VPS4A.
Interacts with PDCD6IP; the interaction is direct.
8 PublicationsBinary interactionsi
Hide detailsQ9BY43
With | #Exp. | IntAct |
---|---|---|
CHMP4B [Q9H444] | 4 | EBI-747981,EBI-749627 |
PDCD6IP [Q8WUM4] | 3 | EBI-747981,EBI-310624 |
SYT17 [Q9BSW7] | 6 | EBI-747981,EBI-745392 |
Isoform 2 [Q9BY43-2]
With | #Exp. | IntAct |
---|---|---|
CHMP4B [Q9H444] | 3 | EBI-12178895,EBI-749627 |
NTAQ1 [Q96HA8] | 3 | EBI-12178895,EBI-741158 |
NUTF2 [P61970] | 3 | EBI-12178895,EBI-591778 |
SYT17 [Q9BSW7] | 3 | EBI-12178895,EBI-745392 |
GO - Molecular functioni
- ATPase binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 118852, 53 interactors |
ComplexPortali | CPX-329, ESCRT-III complex |
CORUMi | Q9BY43 |
DIPi | DIP-39082N |
IntActi | Q9BY43, 25 interactors |
MINTi | Q9BY43 |
STRINGi | 9606.ENSP00000324205 |
Miscellaneous databases
RNActi | Q9BY43, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9BY43 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9BY43 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 150 | Intramolecular interaction with C-terminusBy similarityAdd BLAST | 150 | |
Regioni | 1 – 116 | Interaction with phosphoinosidesAdd BLAST | 116 | |
Regioni | 151 – 222 | Intramolecular interaction with N-terminusBy similarityAdd BLAST | 72 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 20 – 105 | Sequence analysisAdd BLAST | 86 | |
Coiled coili | 155 – 180 | Sequence analysisAdd BLAST | 26 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1656, Eukaryota |
GeneTreei | ENSGT00940000163323 |
HOGENOMi | CLU_071097_0_1_1 |
InParanoidi | Q9BY43 |
OMAi | KQEFLEH |
OrthoDBi | 1490465at2759 |
PhylomeDBi | Q9BY43 |
TreeFami | TF314269 |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGLGRLFGK GKKEKGPTPE EAIQKLKETE KILIKKQEFL EQKIQQELQT
60 70 80 90 100
AKKYGTKNKR AALQALRRKK RFEQQLAQTD GTLSTLEFQR EAIENATTNA
110 120 130 140 150
EVLRTMELAA QSMKKAYQDM DIDKVDELMT DITEQQEVAQ QISDAISRPM
160 170 180 190 200
GFGDDVDEDE LLEELEELEQ EELAQELLNV GDKEEEPSVK LPSVPSTHLP
210 220
AGPAPKVDED EEALKQLAEW VS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PQ80 | E9PQ80_HUMAN | Charged multivesicular body protein... | CHMP4A | 213 | Annotation score: | ||
E9PQI5 | E9PQI5_HUMAN | Charged multivesicular body protein... | CHMP4A | 123 | Annotation score: | ||
H0YIN6 | H0YIN6_HUMAN | Charged multivesicular body protein... | CHMP4A | 117 | Annotation score: | ||
H0YI89 | H0YI89_HUMAN | Charged multivesicular body protein... | CHMP4A | 61 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 16 | G → R in AAF29098 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 66 | L → S in AAF29098 (PubMed:11042152).Curated | 1 | |
Sequence conflicti | 152 – 153 | FG → LLE in AAF29098 (PubMed:11042152).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_023384 | 153 | G → R2 PublicationsCorresponds to variant dbSNP:rs2295322Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056264 | 1 | M → MSRRRPEDGLGKAGPCVMRH HPPRSKAEVWRTLRGGGGRG ELAM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB100262 mRNA Translation: BAC79376.2 AY329084 mRNA Translation: AAQ91193.1 AF212243 mRNA Translation: AAK14928.1 AF161483 mRNA Translation: AAF29098.1 BX161512 mRNA Translation: CAD61949.1 Different initiation. AL096870 Genomic DNA No translation available. AL136295 Genomic DNA No translation available. BC010893 mRNA Translation: AAH10893.2 Different initiation. BC107699 mRNA Translation: AAI07700.1 Different initiation. BC113533 mRNA Translation: AAI13534.1 BC113535 mRNA Translation: AAI13536.1 |
CCDSi | CCDS9619.1 [Q9BY43-1] |
RefSeqi | NP_054888.2, NM_014169.3 [Q9BY43-1] |
Genome annotation databases
Ensembli | ENST00000347519; ENSP00000324205; ENSG00000254505 [Q9BY43-1] ENST00000645308; ENSP00000495982; ENSG00000285302 [Q9BY43-1] |
GeneIDi | 29082 |
KEGGi | hsa:29082 |
UCSCi | uc001wni.4, human [Q9BY43-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB100262 mRNA Translation: BAC79376.2 AY329084 mRNA Translation: AAQ91193.1 AF212243 mRNA Translation: AAK14928.1 AF161483 mRNA Translation: AAF29098.1 BX161512 mRNA Translation: CAD61949.1 Different initiation. AL096870 Genomic DNA No translation available. AL136295 Genomic DNA No translation available. BC010893 mRNA Translation: AAH10893.2 Different initiation. BC107699 mRNA Translation: AAI07700.1 Different initiation. BC113533 mRNA Translation: AAI13534.1 BC113535 mRNA Translation: AAI13536.1 |
CCDSi | CCDS9619.1 [Q9BY43-1] |
RefSeqi | NP_054888.2, NM_014169.3 [Q9BY43-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3C3O | X-ray | 2.15 | B | 210-222 | [»] | |
5MK1 | X-ray | 2.50 | E/F/H/K | 205-222 | [»] | |
SMRi | Q9BY43 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 118852, 53 interactors |
ComplexPortali | CPX-329, ESCRT-III complex |
CORUMi | Q9BY43 |
DIPi | DIP-39082N |
IntActi | Q9BY43, 25 interactors |
MINTi | Q9BY43 |
STRINGi | 9606.ENSP00000324205 |
PTM databases
iPTMneti | Q9BY43 |
PhosphoSitePlusi | Q9BY43 |
Polymorphism and mutation databases
BioMutai | CHMP4A |
DMDMi | 90152096 |
Proteomic databases
EPDi | Q9BY43 |
jPOSTi | Q9BY43 |
MassIVEi | Q9BY43 |
MaxQBi | Q9BY43 |
PaxDbi | Q9BY43 |
PeptideAtlasi | Q9BY43 |
PRIDEi | Q9BY43 |
ProteomicsDBi | 60344 79580 [Q9BY43-1] |
Protocols and materials databases
Antibodypediai | 56042, 37 antibodies |
DNASUi | 29082 |
Genome annotation databases
Ensembli | ENST00000347519; ENSP00000324205; ENSG00000254505 [Q9BY43-1] ENST00000645308; ENSP00000495982; ENSG00000285302 [Q9BY43-1] |
GeneIDi | 29082 |
KEGGi | hsa:29082 |
UCSCi | uc001wni.4, human [Q9BY43-1] |
Organism-specific databases
CTDi | 29082 |
DisGeNETi | 29082 |
EuPathDBi | HostDB:ENSG00000254505.9 |
GeneCardsi | CHMP4A |
HGNCi | HGNC:20274, CHMP4A |
HPAi | ENSG00000254505, Low tissue specificity |
MIMi | 610051, gene |
neXtProti | NX_Q9BY43 |
OpenTargetsi | ENSG00000254505 |
PharmGKBi | PA134888743 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1656, Eukaryota |
GeneTreei | ENSGT00940000163323 |
HOGENOMi | CLU_071097_0_1_1 |
InParanoidi | Q9BY43 |
OMAi | KQEFLEH |
OrthoDBi | 1490465at2759 |
PhylomeDBi | Q9BY43 |
TreeFami | TF314269 |
Enzyme and pathway databases
PathwayCommonsi | Q9BY43 |
Reactomei | R-HSA-162588, Budding and maturation of HIV virion R-HSA-1632852, Macroautophagy R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events R-HSA-9615710, Late endosomal microautophagy R-HSA-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III R-HSA-9679504, Translation of Replicase and Assembly of the Replication Transcription Complex |
SignaLinki | Q9BY43 |
Miscellaneous databases
BioGRID-ORCSi | 29082, 7 hits in 842 CRISPR screens |
ChiTaRSi | CHMP4A, human |
EvolutionaryTracei | Q9BY43 |
GeneWikii | CHMP4A |
GenomeRNAii | 29082 |
Pharosi | Q9BY43, Tbio |
PROi | PR:Q9BY43 |
RNActi | Q9BY43, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000254505, Expressed in frontal cortex and 116 other tissues |
ExpressionAtlasi | Q9BY43, baseline and differential |
Genevisiblei | Q9BY43, HS |
Family and domain databases
InterProi | View protein in InterPro IPR005024, Snf7_fam |
Pfami | View protein in Pfam PF03357, Snf7, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CHM4A_HUMAN | |
Accessioni | Q9BY43Primary (citable) accession number: Q9BY43 Secondary accession number(s): Q14D22 Q9P026 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 2002 |
Last sequence update: | February 7, 2006 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations