UniProtKB - Q9BXS4 (TMM59_HUMAN)
Protein
Transmembrane protein 59
Gene
TMEM59
Organism
Homo sapiens (Human)
Status
Functioni
Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy (PubMed:27273576, PubMed:23376921). Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP (PubMed:20427278). Inhibits APP transport to the cell surface and further shedding (PubMed:20427278).3 Publications
GO - Molecular functioni
- endopeptidase activity Source: Ensembl
GO - Biological processi
- autophagy Source: UniProtKB-KW
- negative regulation of protein glycosylation in Golgi Source: UniProtKB
- negative regulation of protein localization to plasma membrane Source: UniProtKB
- negative regulation of protein processing Source: UniProtKB
- positive regulation of autophagy Source: UniProtKB
Keywordsi
Biological process | Autophagy |
Enzyme and pathway databases
PathwayCommonsi | Q9BXS4 |
Protein family/group databases
TCDBi | 8.A.84.1.4, the insulin secretion regulator tmem59 (tmem59) family |
Names & Taxonomyi
Protein namesi | Recommended name: Transmembrane protein 59Alternative name(s): Liver membrane-bound protein |
Gene namesi | Name:TMEM59 Synonyms:C1orf8 ORF Names:HSPC001, UNQ169/PRO195 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1239, TMEM59 |
neXtProti | NX_Q9BXS4 |
VEuPathDBi | HostDB:ENSG00000116209.11 |
Subcellular locationi
Endosome
- Late endosome membrane 2 Publications; Single-pass type I membrane protein Sequence analysis
Lysosome
- Lysosome membrane 2 Publications; Single-pass type I membrane protein Sequence analysis
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Single-pass type I membrane protein Sequence analysis
Note: Mainly localizes to late endosomes/lysosomes. Probably first exported to the cell surface and then actively endocytosed to transiently localize in early endosomes on its way to the late endosomal/lysosomal compartment where it becomes quickly degraded.1 Publication
Endosome
- late endosome Source: UniProtKB
- late endosome membrane Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi cis cisterna Source: UniProtKB
- Golgi medial cisterna Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
- Golgi trans cisterna Source: UniProtKB
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 36 – 238 | ExtracellularSequence analysisAdd BLAST | 203 | |
Transmembranei | 239 – 259 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 260 – 323 | CytoplasmicSequence analysisAdd BLAST | 64 |
Keywords - Cellular componenti
Cell membrane, Endosome, Golgi apparatus, Lysosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 268 | Y → A or F: Impaired ability to activate LC3. Impaired interaction with ATG16L1. 2 Publications | 1 | |
Mutagenesisi | 268 | Y → W: Does not affect ability to activate LC3. 1 Publication | 1 | |
Mutagenesisi | 269 | V → A: No effect on interaction with ATG16L1. 1 Publication | 1 | |
Mutagenesisi | 272 | E → A or D: Does not affect ability to activate LC3. No effect on interaction with ATG16L1. 2 Publications | 1 | |
Mutagenesisi | 273 | K → A: No effect on interaction with ATG16L1. 1 Publication | 1 | |
Mutagenesisi | 277 | Y → A: Impaired ability to activate LC3. Impaired interaction with ATG16L1. 2 Publications | 1 | |
Mutagenesisi | 277 | Y → W or F: Does not affect ability to activate LC3. 1 Publication | 1 | |
Mutagenesisi | 278 | G → A: No effect on interaction with ATG16L1. 1 Publication | 1 | |
Mutagenesisi | 280 | L → A or V: Impaired ability to activate LC3. Impaired interaction with ATG16L1. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 9528 |
OpenTargetsi | ENSG00000116209 |
PharmGKBi | PA25620 |
Miscellaneous databases
Pharosi | Q9BXS4, Tbio |
Genetic variation databases
BioMutai | TMEM59 |
DMDMi | 18202737 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | Sequence analysisAdd BLAST | 35 | |
ChainiPRO_0000003003 | 36 – 323 | Transmembrane protein 59Add BLAST | 288 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 90 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 303 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q9BXS4 |
jPOSTi | Q9BXS4 |
MassIVEi | Q9BXS4 |
MaxQBi | Q9BXS4 |
PaxDbi | Q9BXS4 |
PeptideAtlasi | Q9BXS4 |
PRIDEi | Q9BXS4 |
ProteomicsDBi | 79491 |
PTM databases
GlyGeni | Q9BXS4, 1 site |
iPTMneti | Q9BXS4 |
PhosphoSitePlusi | Q9BXS4 |
SwissPalmi | Q9BXS4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000116209, Expressed in C1 segment of cervical spinal cord and 248 other tissues |
ExpressionAtlasi | Q9BXS4, baseline and differential |
Genevisiblei | Q9BXS4, HS |
Organism-specific databases
HPAi | ENSG00000116209, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with ATG16L1 (via WD repeats).
2 PublicationsBinary interactionsi
Q9BXS4
With | #Exp. | IntAct |
---|---|---|
ATG16L1 [Q676U5] | 6 | EBI-7054441,EBI-535909 |
Protein-protein interaction databases
BioGRIDi | 114904, 24 interactors |
IntActi | Q9BXS4, 9 interactors |
MINTi | Q9BXS4 |
STRINGi | 9606.ENSP00000234831 |
Miscellaneous databases
RNActi | Q9BXS4, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 263 – 281 | ATG16L1-binding motif2 PublicationsAdd BLAST | 19 |
Domaini
The ATG16L1-binding motif mediates interaction with ATG16L1 and promotes autophagy.1 Publication
Sequence similaritiesi
Belongs to the TMEM59 family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QUIS, Eukaryota |
GeneTreei | ENSGT00390000008279 |
HOGENOMi | CLU_059747_1_0_1 |
InParanoidi | Q9BXS4 |
OMAi | INSSWIL |
OrthoDBi | 1102891at2759 |
PhylomeDBi | Q9BXS4 |
TreeFami | TF331226 |
Family and domain databases
InterProi | View protein in InterPro IPR022065, Uncharacterised_TMEM59 |
PANTHERi | PTHR28652, PTHR28652, 1 hit |
Pfami | View protein in Pfam PF12280, BSMAP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9BXS4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAPKGSLWV RTQLGLPPLL LLTMALAGGS GTASAEAFDS VLGDTASCHR
60 70 80 90 100
ACQLTYPLHT YPKEEELYAC QRGCRLFSIC QFVDDGIDLN RTKLECESAC
110 120 130 140 150
TEAYSQSDEQ YACHLGCQNQ LPFAELRQEQ LMSLMPKMHL LFPLTLVRSF
160 170 180 190 200
WSDMMDSAQS FITSSWTFYL QADDGKIVIF QSKPEIQYAP HLEQEPTNLR
210 220 230 240 250
ESSLSKMSYL QMRNSQAHRN FLEDGESDGF LRCLSLNSGW ILTTTLVLSV
260 270 280 290 300
MVLLWICCAT VATAVEQYVP SEKLSIYGDL EFMNEQKLNR YPASSLVVVR
310 320
SKTEDHEEAG PLPTKVNLAH SEI
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5T6Z8 | Q5T6Z8_HUMAN | Transmembrane protein 59 | TMEM59 hCG_33694 | 192 | Annotation score: | ||
Q5T704 | Q5T704_HUMAN | Transmembrane protein 59 | TMEM59 | 285 | Annotation score: | ||
Q5T706 | Q5T706_HUMAN | Transmembrane protein 59 | TMEM59 | 144 | Annotation score: | ||
Q5T703 | Q5T703_HUMAN | Transmembrane protein 59 | TMEM59 | 145 | Annotation score: |
Sequence cautioni
The sequence AAC39890 differs from that shown. Reason: Frameshift.Curated
The sequence AAH03106 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 209 | Y → SD in AAH16374 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_063397 | 46 | A → V1 PublicationCorresponds to variant dbSNP:rs41294776Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF290615 mRNA Translation: AAK28026.1 AF047439 mRNA Translation: AAC39890.1 Frameshift. AY359029 mRNA Translation: AAQ89388.1 AK075187 mRNA Translation: BAG52079.1 AK075505 mRNA Translation: BAC11658.1 AL353898 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06707.1 BC003106 mRNA Translation: AAH03106.1 Different initiation. BC016374 mRNA Translation: AAH16374.1 BC095464 mRNA Translation: AAH95464.1 |
CCDSi | CCDS586.1 |
RefSeqi | NP_001291972.1, NM_001305043.1 NP_001291978.1, NM_001305049.1 NP_001291979.1, NM_001305050.1 NP_001291980.1, NM_001305051.1 NP_001291981.1, NM_001305052.1 NP_001291995.1, NM_001305066.1 NP_004863.2, NM_004872.4 |
Genome annotation databases
Ensembli | ENST00000234831; ENSP00000234831; ENSG00000116209 |
GeneIDi | 9528 |
KEGGi | hsa:9528 |
UCSCi | uc001cwp.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF290615 mRNA Translation: AAK28026.1 AF047439 mRNA Translation: AAC39890.1 Frameshift. AY359029 mRNA Translation: AAQ89388.1 AK075187 mRNA Translation: BAG52079.1 AK075505 mRNA Translation: BAC11658.1 AL353898 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06707.1 BC003106 mRNA Translation: AAH03106.1 Different initiation. BC016374 mRNA Translation: AAH16374.1 BC095464 mRNA Translation: AAH95464.1 |
CCDSi | CCDS586.1 |
RefSeqi | NP_001291972.1, NM_001305043.1 NP_001291978.1, NM_001305049.1 NP_001291979.1, NM_001305050.1 NP_001291980.1, NM_001305051.1 NP_001291981.1, NM_001305052.1 NP_001291995.1, NM_001305066.1 NP_004863.2, NM_004872.4 |
3D structure databases
SMRi | Q9BXS4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 114904, 24 interactors |
IntActi | Q9BXS4, 9 interactors |
MINTi | Q9BXS4 |
STRINGi | 9606.ENSP00000234831 |
Protein family/group databases
TCDBi | 8.A.84.1.4, the insulin secretion regulator tmem59 (tmem59) family |
PTM databases
GlyGeni | Q9BXS4, 1 site |
iPTMneti | Q9BXS4 |
PhosphoSitePlusi | Q9BXS4 |
SwissPalmi | Q9BXS4 |
Genetic variation databases
BioMutai | TMEM59 |
DMDMi | 18202737 |
Proteomic databases
EPDi | Q9BXS4 |
jPOSTi | Q9BXS4 |
MassIVEi | Q9BXS4 |
MaxQBi | Q9BXS4 |
PaxDbi | Q9BXS4 |
PeptideAtlasi | Q9BXS4 |
PRIDEi | Q9BXS4 |
ProteomicsDBi | 79491 |
Protocols and materials databases
Antibodypediai | 46893, 86 antibodies |
DNASUi | 9528 |
Genome annotation databases
Ensembli | ENST00000234831; ENSP00000234831; ENSG00000116209 |
GeneIDi | 9528 |
KEGGi | hsa:9528 |
UCSCi | uc001cwp.4, human |
Organism-specific databases
CTDi | 9528 |
DisGeNETi | 9528 |
GeneCardsi | TMEM59 |
HGNCi | HGNC:1239, TMEM59 |
HPAi | ENSG00000116209, Low tissue specificity |
neXtProti | NX_Q9BXS4 |
OpenTargetsi | ENSG00000116209 |
PharmGKBi | PA25620 |
VEuPathDBi | HostDB:ENSG00000116209.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QUIS, Eukaryota |
GeneTreei | ENSGT00390000008279 |
HOGENOMi | CLU_059747_1_0_1 |
InParanoidi | Q9BXS4 |
OMAi | INSSWIL |
OrthoDBi | 1102891at2759 |
PhylomeDBi | Q9BXS4 |
TreeFami | TF331226 |
Enzyme and pathway databases
PathwayCommonsi | Q9BXS4 |
Miscellaneous databases
BioGRID-ORCSi | 9528, 1 hit in 863 CRISPR screens |
ChiTaRSi | TMEM59, human |
GeneWikii | TMEM59 |
GenomeRNAii | 9528 |
Pharosi | Q9BXS4, Tbio |
PROi | PR:Q9BXS4 |
RNActi | Q9BXS4, protein |
Gene expression databases
Bgeei | ENSG00000116209, Expressed in C1 segment of cervical spinal cord and 248 other tissues |
ExpressionAtlasi | Q9BXS4, baseline and differential |
Genevisiblei | Q9BXS4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR022065, Uncharacterised_TMEM59 |
PANTHERi | PTHR28652, PTHR28652, 1 hit |
Pfami | View protein in Pfam PF12280, BSMAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TMM59_HUMAN | |
Accessioni | Q9BXS4Primary (citable) accession number: Q9BXS4 Secondary accession number(s): B3KQL7 Q96KX7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - SIMILARITY comments
Index of protein domains and families