TMEM59

Functionⁱ

Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy (PubMed:27273576, PubMed:23376921). Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP (PubMed:20427278). Inhibits APP transport to the cell surface and further shedding (PubMed:20427278).3 Publications

GO - Molecular functionⁱ

endopeptidase activity Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

autophagy Source: UniProtKB-KW
negative regulation of protein glycosylation in Golgi
Source: UniProtKB
Inferred from direct assayⁱ
- 20427278
negative regulation of protein localization to plasma membrane
Source: UniProtKB
Inferred from direct assayⁱ
- 20427278
negative regulation of protein processing
Source: UniProtKB
Inferred from direct assayⁱ
- 20427278
positive regulation of autophagy
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23376921

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Autophagy

Biological process

Autophagy

Protein family/group databases

TCDBⁱ	8.A.84.1.4 the insulin secretion regulator tmem59 (tmem59) family

TCDBⁱ

8.A.84.1.4 the insulin secretion regulator tmem59 (tmem59) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transmembrane protein 59 Alternative name(s): Liver membrane-bound protein
Gene namesⁱ	Name:TMEM59 Synonyms:C1orf8 ORF Names:HSPC001, UNQ169/PRO195
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000116209.11
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:1239 TMEM59
neXtProtⁱ	NX_Q9BXS4

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	36 – 238	ExtracellularSequence analysisAdd BLAST	203
Transmembraneⁱ	239 – 259	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	260 – 323	CytoplasmicSequence analysisAdd BLAST	64

Keywords - Cellular componentⁱ

Cell membrane, Endosome, Golgi apparatus, Lysosome, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	268	Y → A or F: Impaired ability to activate LC3. Impaired interaction with ATG16L1. 2 Publications	1
Mutagenesisⁱ	268	Y → W: Does not affect ability to activate LC3. 1 Publication	1
Mutagenesisⁱ	269	V → A: No effect on interaction with ATG16L1. 1 Publication	1
Mutagenesisⁱ	272	E → A or D: Does not affect ability to activate LC3. No effect on interaction with ATG16L1. 2 Publications	1
Mutagenesisⁱ	273	K → A: No effect on interaction with ATG16L1. 1 Publication	1
Mutagenesisⁱ	277	Y → A: Impaired ability to activate LC3. Impaired interaction with ATG16L1. 2 Publications	1
Mutagenesisⁱ	277	Y → W or F: Does not affect ability to activate LC3. 1 Publication	1
Mutagenesisⁱ	278	G → A: No effect on interaction with ATG16L1. 1 Publication	1
Mutagenesisⁱ	280	L → A or V: Impaired ability to activate LC3. Impaired interaction with ATG16L1. 2 Publications	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9528
Open Targets More...OpenTargetsⁱ	ENSG00000116209
PharmGKBⁱ	PA25620

Polymorphism and mutation databases

BioMutaⁱ	TMEM59
DMDMⁱ	18202737

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 35	Sequence analysisAdd BLAST		35
ChainⁱPRO_0000003003	36 – 323	Transmembrane protein 59Add BLAST		288

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	90	N-linked (GlcNAc...) asparagine1 Publication		1
Modified residueⁱ	303	PhosphothreonineBy similarity		1

Post-translational modificationⁱ

N-glycosylated.1 Publication

Keywords - PTMⁱ

Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	Q9BXS4
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9BXS4
PaxDbⁱ	Q9BXS4
PeptideAtlas More...PeptideAtlasⁱ	Q9BXS4
PRIDEⁱ	Q9BXS4
ProteomicsDBⁱ	79491

PTM databases

iPTMnetⁱ	Q9BXS4
PhosphoSitePlusⁱ	Q9BXS4
SwissPalmⁱ	Q9BXS4

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000116209 Expressed in 236 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExⁱ	HS_TMEM59
ExpressionAtlasⁱ	Q9BXS4 baseline and differential
Genevisibleⁱ	Q9BXS4 HS

Interactionⁱ

Subunit structureⁱ

Interacts with ATG16L1 (via WD repeats).2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ATG16L1	Q676U5	6	EBI-7054441,EBI-535909

With

Entry

#Exp.

IntAct

Notes

ATG16L1

Q676U5

6

EBI-7054441,EBI-535909

Protein-protein interaction databases

BioGridⁱ	114904, 11 interactors
IntActⁱ	Q9BXS4, 6 interactors
Molecular INTeraction database More...MINTⁱ	Q9BXS4
STRINGⁱ	9606.ENSP00000234831

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9BXS4
SMRⁱ	Q9BXS4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	263 – 281	ATG16L1-binding motif2 PublicationsAdd BLAST		19

Domainⁱ

The ATG16L1-binding motif mediates interaction with ATG16L1 and promotes autophagy.1 Publication

Sequence similaritiesⁱ

Belongs to the TMEM59 family.Curated

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IIST Eukaryota ENOG410XTCW LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000008279
HOVERGENⁱ	HBG018206
InParanoidⁱ	Q9BXS4
OMAⁱ	QEPTNLK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0EVN
PhylomeDBⁱ	Q9BXS4
TreeFamⁱ	TF331226

Family and domain databases

InterProⁱ	View protein in InterPro IPR022065 Uncharacterised_TMEM59
PANTHERⁱ	PTHR28652 PTHR28652, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12280 BSMAP, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show all Align All

Q9BXS4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAPKGSLWV RTQLGLPPLL LLTMALAGGS GTASAEAFDS VLGDTASCHR 
        60         70         80         90        100
ACQLTYPLHT YPKEEELYAC QRGCRLFSIC QFVDDGIDLN RTKLECESAC 
       110        120        130        140        150
TEAYSQSDEQ YACHLGCQNQ LPFAELRQEQ LMSLMPKMHL LFPLTLVRSF 
       160        170        180        190        200
WSDMMDSAQS FITSSWTFYL QADDGKIVIF QSKPEIQYAP HLEQEPTNLR 
       210        220        230        240        250
ESSLSKMSYL QMRNSQAHRN FLEDGESDGF LRCLSLNSGW ILTTTLVLSV 
       260        270        280        290        300
MVLLWICCAT VATAVEQYVP SEKLSIYGDL EFMNEQKLNR YPASSLVVVR 
       310        320 
SKTEDHEEAG PLPTKVNLAH SEI

Length:323

Mass (Da):36,223

Last modified:June 1, 2001 - v1

Checksum:ⁱ0926AB7D12D1B902

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

Q5T6Z8	Q5T6Z8_HUMAN	Transmembrane protein 59, isoform C... Transmembrane protein 59, isoform CRA_a	TMEM59 hCG_33694	192	Annotation score:
Q5T704	Q5T704_HUMAN	Transmembrane protein 59 Transmembrane protein 59	TMEM59	285	Annotation score:
Q5T706	Q5T706_HUMAN	Transmembrane protein 59 Transmembrane protein 59	TMEM59	144	Annotation score:
Q5T703	Q5T703_HUMAN	Transmembrane protein 59 Transmembrane protein 59	TMEM59	145	Annotation score:

Sequence cautionⁱ

The sequence AAC39890 differs from that shown. Reason: Frameshift at position 6.Curated

The sequence AAH03106 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	209	Y → SD in AAH16374 (PubMed:15489334).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_063397	46	A → V1 PublicationCorresponds to variant dbSNP:rs41294776Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF290615 mRNA Translation: AAK28026.1 AF047439 mRNA Translation: AAC39890.1 Frameshift. AY359029 mRNA Translation: AAQ89388.1 AK075187 mRNA Translation: BAG52079.1 AK075505 mRNA Translation: BAC11658.1 AL353898 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06707.1 BC003106 mRNA Translation: AAH03106.1 Different initiation. BC016374 mRNA Translation: AAH16374.1 BC095464 mRNA Translation: AAH95464.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS586.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001291972.1, NM_001305043.1 NP_001291978.1, NM_001305049.1 NP_001291979.1, NM_001305050.1 NP_001291980.1, NM_001305051.1 NP_001291981.1, NM_001305052.1 NP_001291995.1, NM_001305066.1 NP_004863.2, NM_004872.4
UniGeneⁱ	Hs.118947 Hs.523262 Hs.558618 Hs.684693 Hs.689423

Genome annotation databases

Ensemblⁱ	ENST00000234831; ENSP00000234831; ENSG00000116209
GeneIDⁱ	9528
KEGGⁱ	hsa:9528
UCSC genome browser More...UCSCⁱ	uc001cwp.4 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9BXS4	TMEM59 isoform 3		PANTR		323	UniRef100_Q9BXS4

Protein	Similar proteins		Species	Score	Length	Source
Q9BXS4	Transmembrane protein 59		BOVIN		323	UniRef90_Q9BXS4
Transmembrane protein 59		MACFA		323
Transmembrane protein 59		MOUSE		323
Transmembrane protein 59		PIG		323
Transmembrane protein 59		PONAB		323
+230

Protein	Similar proteins		Species	Score	Length	Source
Q9BXS4	Transmembrane protein 59		MACFA		323	UniRef50_Q9BXS4
Transmembrane protein 59		PIG		323
Transmembrane protein 59		BOVIN		323
Transmembrane protein 59		PONAB		323
Transmembrane protein 59		MOUSE		323
+520

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF290615 mRNA Translation: AAK28026.1 AF047439 mRNA Translation: AAC39890.1 Frameshift. AY359029 mRNA Translation: AAQ89388.1 AK075187 mRNA Translation: BAG52079.1 AK075505 mRNA Translation: BAC11658.1 AL353898 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06707.1 BC003106 mRNA Translation: AAH03106.1 Different initiation. BC016374 mRNA Translation: AAH16374.1 BC095464 mRNA Translation: AAH95464.1
CCDSⁱ	CCDS586.1
RefSeqⁱ	NP_001291972.1, NM_001305043.1 NP_001291978.1, NM_001305049.1 NP_001291979.1, NM_001305050.1 NP_001291980.1, NM_001305051.1 NP_001291981.1, NM_001305052.1 NP_001291995.1, NM_001305066.1 NP_004863.2, NM_004872.4
UniGeneⁱ	Hs.118947 Hs.523262 Hs.558618 Hs.684693 Hs.689423

3D structure databases

ProteinModelPortalⁱ	Q9BXS4
SMRⁱ	Q9BXS4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	114904, 11 interactors
IntActⁱ	Q9BXS4, 6 interactors
MINTⁱ	Q9BXS4
STRINGⁱ	9606.ENSP00000234831

Protein family/group databases

TCDBⁱ	8.A.84.1.4 the insulin secretion regulator tmem59 (tmem59) family

TCDBⁱ

8.A.84.1.4 the insulin secretion regulator tmem59 (tmem59) family

PTM databases

iPTMnetⁱ	Q9BXS4
PhosphoSitePlusⁱ	Q9BXS4
SwissPalmⁱ	Q9BXS4

Polymorphism and mutation databases

BioMutaⁱ	TMEM59
DMDMⁱ	18202737

Proteomic databases

EPDⁱ	Q9BXS4
MaxQBⁱ	Q9BXS4
PaxDbⁱ	Q9BXS4
PeptideAtlasⁱ	Q9BXS4
PRIDEⁱ	Q9BXS4
ProteomicsDBⁱ	79491

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9528
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000234831; ENSP00000234831; ENSG00000116209
GeneIDⁱ	9528
KEGGⁱ	hsa:9528
UCSCⁱ	uc001cwp.4 human

Organism-specific databases

CTDⁱ	9528
DisGeNETⁱ	9528
EuPathDBⁱ	HostDB:ENSG00000116209.11
GeneCardsⁱ	TMEM59
HGNCⁱ	HGNC:1239 TMEM59
neXtProtⁱ	NX_Q9BXS4
OpenTargetsⁱ	ENSG00000116209
PharmGKBⁱ	PA25620
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IIST Eukaryota ENOG410XTCW LUCA
GeneTreeⁱ	ENSGT00390000008279
HOVERGENⁱ	HBG018206
InParanoidⁱ	Q9BXS4
OMAⁱ	QEPTNLK
OrthoDBⁱ	EOG091G0EVN
PhylomeDBⁱ	Q9BXS4
TreeFamⁱ	TF331226

Miscellaneous databases

ChiTaRSⁱ	TMEM59 human
GeneWikiⁱ	TMEM59
GenomeRNAiⁱ	9528
Protein Ontology More...PROⁱ	PR:Q9BXS4

Gene expression databases

Bgeeⁱ	ENSG00000116209 Expressed in 236 organ(s), highest expression level in C1 segment of cervical spinal cord
CleanExⁱ	HS_TMEM59
ExpressionAtlasⁱ	Q9BXS4 baseline and differential
Genevisibleⁱ	Q9BXS4 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR022065 Uncharacterised_TMEM59
PANTHERⁱ	PTHR28652 PTHR28652, 1 hit
Pfamⁱ	View protein in Pfam PF12280 BSMAP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TMM59_HUMAN
Accessionⁱ	Q9BXS4Primary (citable) accession number: Q9BXS4 Secondary accession number(s): B3KQL7 , O75393, Q4VBP9, Q5T705, Q96KX7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
	Last sequence update:	June 1, 2001
	Last modified:	November 7, 2018
	This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations

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UniProtKB - Q9BXS4 (TMM59_HUMAN)

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Transmembrane protein 59

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endosome

Lysosome

Plasma membrane

Golgi apparatus

Endosome

Extracellular region or secreted

Golgi apparatus

Lysosome

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ