UniProtKB - Q9BXR0 (TGT_HUMAN)
Protein
Queuine tRNA-ribosyltransferase catalytic subunit 1
Gene
QTRT1
Organism
Homo sapiens (Human)
Status
Functioni
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).UniRule annotationBy similarity2 Publications
Catalytic activityi
- EC:2.4.2.29UniRule annotation1 Publication
Cofactori
Zn2+UniRule annotation1 Publication
Kineticsi
kcat is 0.0056 sec(-1) with tRNA(Tyr) as substrate and 0.00586 sec(-1) with guanine as substrate.1 Publication
- KM=0.34 µM for tRNA(Tyr)1 Publication
- KM=0.41 µM for guanine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 105 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 159 | SubstrateUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 202 | SubstrateUniRule annotation | 1 | |
Binding sitei | 229 | Substrate; via amide nitrogenUniRule annotationCombined sources1 Publication | 1 | |
Active sitei | 279 | NucleophileUniRule annotation | 1 | |
Metal bindingi | 317 | ZincUniRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 319 | ZincUniRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 322 | ZincUniRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 348 | Zinc; via pros nitrogenUniRule annotationCombined sources1 Publication | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- queuine tRNA-ribosyltransferase activity Source: UniProtKB
GO - Biological processi
- tRNA-guanine transglycosylation Source: UniProtKB
- tRNA modification Source: GO_Central
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05270-MONOMER |
BRENDAi | 2.4.2.29, 2681 |
PathwayCommonsi | Q9BXR0 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: Queuine tRNA-ribosyltransferase catalytic subunit 1UniRule annotation (EC:2.4.2.29UniRule annotation1 Publication)Alternative name(s): Guanine insertion enzymeUniRule annotation tRNA-guanine transglycosylaseUniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000213339.8 |
HGNCi | HGNC:23797, QTRT1 |
MIMi | 609615, gene |
neXtProti | NX_Q9BXR0 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Other locations
- Cytoplasm UniRule annotation
Note: Weakly associates with mitochondria, possibly via QTRT2.UniRule annotation
Mitochondrion
- mitochondrial outer membrane Source: Reactome
Nucleus
- nucleus Source: Ensembl
Other locations
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 279 | D → N: Loss of transglycosylase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 81890 |
OpenTargetsi | ENSG00000213339 |
PharmGKBi | PA134963074 |
Miscellaneous databases
Pharosi | Q9BXR0, Tbio |
Chemistry databases
ChEMBLi | CHEMBL6004 |
Polymorphism and mutation databases
BioMutai | QTRT1 |
DMDMi | 239938842 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000135565 | 2 – 403 | Queuine tRNA-ribosyltransferase catalytic subunit 1Add BLAST | 402 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 139 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9BXR0 |
jPOSTi | Q9BXR0 |
MassIVEi | Q9BXR0 |
MaxQBi | Q9BXR0 |
PaxDbi | Q9BXR0 |
PeptideAtlasi | Q9BXR0 |
PRIDEi | Q9BXR0 |
ProteomicsDBi | 4060 79483 [Q9BXR0-1] |
PTM databases
iPTMneti | Q9BXR0 |
PhosphoSitePlusi | Q9BXR0 |
Expressioni
Gene expression databases
Bgeei | ENSG00000213339, Expressed in body of pancreas and 192 other tissues |
ExpressionAtlasi | Q9BXR0, baseline and differential |
Genevisiblei | Q9BXR0, HS |
Organism-specific databases
HPAi | ENSG00000213339, Low tissue specificity |
Interactioni
Subunit structurei
Heterodimer of a catalytic subunit QTRT1 and an accessory subunit QTRT2.
UniRule annotation2 PublicationsGO - Molecular functioni
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 123623, 19 interactors |
IntActi | Q9BXR0, 16 interactors |
STRINGi | 9606.ENSP00000250237 |
Chemistry databases
BindingDBi | Q9BXR0 |
Miscellaneous databases
RNActi | Q9BXR0, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9BXR0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 105 – 109 | Substrate bindingUniRule annotationCombined sources1 Publication | 5 | |
Regioni | 260 – 266 | RNA bindingUniRule annotation | 7 | |
Regioni | 284 – 288 | RNA binding; important for wobble base 34 recognitionUniRule annotation | 5 |
Sequence similaritiesi
Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG3908, Eukaryota |
GeneTreei | ENSGT00530000063679 |
HOGENOMi | CLU_022060_0_1_1 |
InParanoidi | Q9BXR0 |
OMAi | GIDLFDC |
PhylomeDBi | Q9BXR0 |
TreeFami | TF300732 |
Family and domain databases
Gene3Di | 3.20.20.105, 1 hit |
HAMAPi | MF_00168, Q_tRNA_Tgt, 1 hit |
InterProi | View protein in InterPro IPR004803, TGT IPR036511, TGT-like_sf IPR002616, tRNA_ribo_trans-like |
Pfami | View protein in Pfam PF01702, TGT, 1 hit |
SUPFAMi | SSF51713, SSF51713, 1 hit |
TIGRFAMsi | TIGR00430, Q_tRNA_tgt, 1 hit TIGR00449, tgt_general, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9BXR0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGAATQASL ESAPRIMRLV AECSRSRARA GELWLPHGTV ATPVFMPVGT
60 70 80 90 100
QATMKGITTE QLDALGCRIC LGNTYHLGLR PGPELIQKAN GLHGFMNWPH
110 120 130 140 150
NLLTDSGGFQ MVSLVSLSEV TEEGVRFRSP YDGNETLLSP EKSVQIQNAL
160 170 180 190 200
GSDIIMQLDD VVSSTVTGPR VEEAMYRSIR WLDRCIAAHQ RPDKQNLFAI
210 220 230 240 250
IQGGLDADLR ATCLEEMTKR DVPGFAIGGL SGGESKSQFW RMVALSTSRL
260 270 280 290 300
PKDKPRYLMG VGYATDLVVC VALGCDMFDC VFPTRTARFG SALVPTGNLQ
310 320 330 340 350
LRKKVFEKDF GPIDPECTCP TCQKHSRAFL HALLHSDNTA ALHHLTVHNI
360 370 380 390 400
AYQLQLMSAV RTSIVEKRFP DFVRDFMGAM YGDPTLCPTW ATDALASVGI
TLG
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EPI1 | K7EPI1_HUMAN | Queuine tRNA-ribosyltransferase cat... | QTRT1 | 124 | Annotation score: | ||
K7EL95 | K7EL95_HUMAN | Queuine tRNA-ribosyltransferase cat... | QTRT1 | 136 | Annotation score: | ||
K7ESP6 | K7ESP6_HUMAN | Queuine tRNA-ribosyltransferase cat... | QTRT1 | 180 | Annotation score: | ||
K7EJ21 | K7EJ21_HUMAN | Queuine tRNA-ribosyltransferase cat... | QTRT1 | 203 | Annotation score: |
Sequence cautioni
The sequence AAH15350 differs from that shown. Reason: Erroneous initiation.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056470 | 178 – 225 | SIRWL…DVPGF → PLGLVTGALLQVNPLAGPVH CSPSAAGQAEPLRHYPGWAG RRSPGHLP in isoform 2. 1 PublicationAdd BLAST | 48 | |
Alternative sequenceiVSP_056471 | 226 – 403 | Missing in isoform 2. 1 PublicationAdd BLAST | 178 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF302783 mRNA Translation: AAG60033.1 AF302784 mRNA Translation: AAG60034.1 AK294166 mRNA Translation: BAG57488.1 AC011475 Genomic DNA No translation available. BC015350 mRNA Translation: AAH15350.1 Different initiation. |
CCDSi | CCDS12248.1 [Q9BXR0-1] |
RefSeqi | NP_112486.1, NM_031209.2 [Q9BXR0-1] |
Genome annotation databases
Ensembli | ENST00000250237; ENSP00000250237; ENSG00000213339 [Q9BXR0-1] ENST00000421333; ENSP00000389126; ENSG00000213339 [Q9BXR0-2] |
GeneIDi | 81890 |
KEGGi | hsa:81890 |
UCSCi | uc002mpr.4, human [Q9BXR0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF302783 mRNA Translation: AAG60033.1 AF302784 mRNA Translation: AAG60034.1 AK294166 mRNA Translation: BAG57488.1 AC011475 Genomic DNA No translation available. BC015350 mRNA Translation: AAH15350.1 Different initiation. |
CCDSi | CCDS12248.1 [Q9BXR0-1] |
RefSeqi | NP_112486.1, NM_031209.2 [Q9BXR0-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6H42 | X-ray | 2.45 | A/B | 11-403 | [»] | |
6H45 | X-ray | 2.40 | A/B | 12-403 | [»] | |
SMRi | Q9BXR0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123623, 19 interactors |
IntActi | Q9BXR0, 16 interactors |
STRINGi | 9606.ENSP00000250237 |
Chemistry databases
BindingDBi | Q9BXR0 |
ChEMBLi | CHEMBL6004 |
PTM databases
iPTMneti | Q9BXR0 |
PhosphoSitePlusi | Q9BXR0 |
Polymorphism and mutation databases
BioMutai | QTRT1 |
DMDMi | 239938842 |
Proteomic databases
EPDi | Q9BXR0 |
jPOSTi | Q9BXR0 |
MassIVEi | Q9BXR0 |
MaxQBi | Q9BXR0 |
PaxDbi | Q9BXR0 |
PeptideAtlasi | Q9BXR0 |
PRIDEi | Q9BXR0 |
ProteomicsDBi | 4060 79483 [Q9BXR0-1] |
Protocols and materials databases
Antibodypediai | 25490, 149 antibodies |
DNASUi | 81890 |
Genome annotation databases
Ensembli | ENST00000250237; ENSP00000250237; ENSG00000213339 [Q9BXR0-1] ENST00000421333; ENSP00000389126; ENSG00000213339 [Q9BXR0-2] |
GeneIDi | 81890 |
KEGGi | hsa:81890 |
UCSCi | uc002mpr.4, human [Q9BXR0-1] |
Organism-specific databases
CTDi | 81890 |
DisGeNETi | 81890 |
EuPathDBi | HostDB:ENSG00000213339.8 |
GeneCardsi | QTRT1 |
HGNCi | HGNC:23797, QTRT1 |
HPAi | ENSG00000213339, Low tissue specificity |
MIMi | 609615, gene |
neXtProti | NX_Q9BXR0 |
OpenTargetsi | ENSG00000213339 |
PharmGKBi | PA134963074 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3908, Eukaryota |
GeneTreei | ENSGT00530000063679 |
HOGENOMi | CLU_022060_0_1_1 |
InParanoidi | Q9BXR0 |
OMAi | GIDLFDC |
PhylomeDBi | Q9BXR0 |
TreeFami | TF300732 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS05270-MONOMER |
BRENDAi | 2.4.2.29, 2681 |
PathwayCommonsi | Q9BXR0 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 81890, 20 hits in 848 CRISPR screens |
ChiTaRSi | QTRT1, human |
GenomeRNAii | 81890 |
Pharosi | Q9BXR0, Tbio |
PROi | PR:Q9BXR0 |
RNActi | Q9BXR0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000213339, Expressed in body of pancreas and 192 other tissues |
ExpressionAtlasi | Q9BXR0, baseline and differential |
Genevisiblei | Q9BXR0, HS |
Family and domain databases
Gene3Di | 3.20.20.105, 1 hit |
HAMAPi | MF_00168, Q_tRNA_Tgt, 1 hit |
InterProi | View protein in InterPro IPR004803, TGT IPR036511, TGT-like_sf IPR002616, tRNA_ribo_trans-like |
Pfami | View protein in Pfam PF01702, TGT, 1 hit |
SUPFAMi | SSF51713, SSF51713, 1 hit |
TIGRFAMsi | TIGR00430, Q_tRNA_tgt, 1 hit TIGR00449, tgt_general, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TGT_HUMAN | |
Accessioni | Q9BXR0Primary (citable) accession number: Q9BXR0 Secondary accession number(s): B4DFM7, Q96BQ4, Q9BXQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2002 |
Last sequence update: | June 16, 2009 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM