Bcl-2-binding component 3, isoforms 1/2

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processⁱ

• activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • Ref.2

    "PUMA, a novel proapoptotic gene, is induced by p53."
    Nakano K., Wousden K.H.
    Mol. Cell 7:683-694(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), FUNCTION, INTERACTION WITH BCL2, INDUCTION BY TP53, SUBCELLULAR LOCATION, MUTAGENESIS OF 141-LEU--ARG-143.
  • "Differential contribution of Puma and Noxa in dual regulation of p53-mediated apoptotic pathways."
    Shibue T., Suzuki S., Okamoto H., Yoshida H., Ohba Y., Takaoka A., Taniguchi T.
    EMBO J. 25:4952-4962(2006) [PubMed] [Europe PMC] [Abstract]
• apoptotic signaling pathway Source: UniProtKBInferred from direct assayⁱ
  • Ref.2

    "PUMA, a novel proapoptotic gene, is induced by p53."
    Nakano K., Wousden K.H.
    Mol. Cell 7:683-694(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), FUNCTION, INTERACTION WITH BCL2, INDUCTION BY TP53, SUBCELLULAR LOCATION, MUTAGENESIS OF 141-LEU--ARG-143.
• cellular response to DNA damage stimulus Source: BHF-UCL
• cellular response to hypoxia Source: UniProtKB <p>Inferred from Expression Pattern</p> <p>Covers cases where the annotation is inferred from the timing or location of expression of a gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iep">GO evidence code guide</a></p> Inferred from expression patternⁱ
  • "Hypoxia downregulates p53 but induces apoptosis and enhances expression of BAD in cultures of human syncytiotrophoblasts."
    Chen B., Longtine M.S., Sadovsky Y., Nelson D.M.
    Am. J. Physiol., Cell Physiol. 299:C968-76(2010) [PubMed] [Europe PMC] [Abstract]
• determination of adult lifespan Source: BHF-UCL
• execution phase of apoptosis Source: UniProtKBInferred from direct assayⁱ
  • Ref.1

    "PUMA induces the rapid apoptosis of colorectal cancer cells."
    Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B.
    Mol. Cell 7:673-682(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BCL2 AND BCL2L1, TISSUE SPECIFICITY.
  • Ref.2

    "PUMA, a novel proapoptotic gene, is induced by p53."
    Nakano K., Wousden K.H.
    Mol. Cell 7:683-694(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), FUNCTION, INTERACTION WITH BCL2, INDUCTION BY TP53, SUBCELLULAR LOCATION, MUTAGENESIS OF 141-LEU--ARG-143.
• intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCLInferred from direct assayⁱ
  • "Endoplasmic reticulum stress-induced apoptosis: multiple pathways and activation of p53-up-regulated modulator of apoptosis (PUMA) and NOXA by p53."
    Li J., Lee B., Lee A.S.
    J. Biol. Chem. 281:7260-7270(2006) [PubMed] [Europe PMC] [Abstract]
• negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKBInferred from direct assayⁱ
  • "Differential contribution of Puma and Noxa in dual regulation of p53-mediated apoptotic pathways."
    Shibue T., Suzuki S., Okamoto H., Yoshida H., Ohba Y., Takaoka A., Taniguchi T.
    EMBO J. 25:4952-4962(2006) [PubMed] [Europe PMC] [Abstract]
• negative regulation of growth Source: UniProtKB <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • Ref.2

    "PUMA, a novel proapoptotic gene, is induced by p53."
    Nakano K., Wousden K.H.
    Mol. Cell 7:683-694(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), FUNCTION, INTERACTION WITH BCL2, INDUCTION BY TP53, SUBCELLULAR LOCATION, MUTAGENESIS OF 141-LEU--ARG-143.
• positive regulation of cysteine-type endopeptidase activity Source: UniProtKB
• positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL <p>Traceable Author Statement</p> <p>Used for information from review articles where the original experiments are traceable through that article and also for information from text books or dictionaries.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#tas">GO evidence code guide</a></p> Traceable author statementⁱ
  • "The unfolded protein response: integrating stress signals through the stress sensor IRE1alpha."
    Hetz C., Martinon F., Rodriguez D., Glimcher L.H.
    Physiol. Rev. 91:1219-1243(2011) [PubMed] [Europe PMC] [Abstract]
• positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKBInferred from mutant phenotypeⁱ
  • "Apoptosis initiated when BH3 ligands engage multiple Bcl-2 homologs, not Bax or Bak."
    Willis S.N., Fletcher J.I., Kaufmann T., van Delft M.F., Chen L., Czabotar P.E., Ierino H., Lee E.F., Fairlie W.D., Bouillet P., Strasser A., Kluck R.M., Adams J.M., Huang D.C.
    Science 315:856-859(2007) [PubMed] [Europe PMC] [Abstract]
  • "Nupr1/Chop signal axis is involved in mitochondrion-related endothelial cell apoptosis induced by methamphetamine."
    Cai D., Huang E., Luo B., Yang Y., Zhang F., Liu C., Lin Z., Xie W.B., Wang H.
    Cell Death Dis. 7:E2161-E2161(2016) [PubMed] [Europe PMC] [Abstract]
• positive regulation of IRE1-mediated unfolded protein response Source: ParkinsonsUK-UCLTraceable author statementⁱ
  • "The unfolded protein response: integrating stress signals through the stress sensor IRE1alpha."
    Hetz C., Martinon F., Rodriguez D., Glimcher L.H.
    Physiol. Rev. 91:1219-1243(2011) [PubMed] [Europe PMC] [Abstract]
• positive regulation of neuron apoptotic process Source: UniProtKB
• positive regulation of protein-containing complex assembly Source: UniProtKBInferred from direct assayⁱ
  • "Differential contribution of Puma and Noxa in dual regulation of p53-mediated apoptotic pathways."
    Shibue T., Suzuki S., Okamoto H., Yoshida H., Ohba Y., Takaoka A., Taniguchi T.
    EMBO J. 25:4952-4962(2006) [PubMed] [Europe PMC] [Abstract]
• positive regulation of protein homooligomerization Source: UniProtKBInferred from direct assayⁱ
  • "Differential contribution of Puma and Noxa in dual regulation of p53-mediated apoptotic pathways."
    Shibue T., Suzuki S., Okamoto H., Yoshida H., Ohba Y., Takaoka A., Taniguchi T.
    EMBO J. 25:4952-4962(2006) [PubMed] [Europe PMC] [Abstract]
• positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: UniProtKBInferred from direct assayⁱ
  • "Differential contribution of Puma and Noxa in dual regulation of p53-mediated apoptotic pathways."
    Shibue T., Suzuki S., Okamoto H., Yoshida H., Ohba Y., Takaoka A., Taniguchi T.
    EMBO J. 25:4952-4962(2006) [PubMed] [Europe PMC] [Abstract]
• positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL <p>Inferred from Genetic Interaction</p> <p>Used to describe "traditional" genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "BH3 domains other than Bim and Bid can directly activate Bax/Bak."
    Du H., Wolf J., Schafer B., Moldoveanu T., Chipuk J.E., Kuwana T.
    J. Biol. Chem. 286:491-501(2011) [PubMed] [Europe PMC] [Abstract]
• positive regulation of thymocyte apoptotic process Source: BHF-UCL
• protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
• regulation of apoptotic process Source: Reactome
• release of cytochrome c from mitochondria Source: UniProtKBInferred from direct assayⁱ
  • Ref.2

    "PUMA, a novel proapoptotic gene, is induced by p53."
    Nakano K., Wousden K.H.
    Mol. Cell 7:683-694(2001) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), FUNCTION, INTERACTION WITH BCL2, INDUCTION BY TP53, SUBCELLULAR LOCATION, MUTAGENESIS OF 141-LEU--ARG-143.
• release of sequestered calcium ion into cytosol Source: UniProtKBInferred from direct assayⁱ
  • "Differential contribution of Puma and Noxa in dual regulation of p53-mediated apoptotic pathways."
    Shibue T., Suzuki S., Okamoto H., Yoshida H., Ohba Y., Takaoka A., Taniguchi T.
    EMBO J. 25:4952-4962(2006) [PubMed] [Europe PMC] [Abstract]
• response to endoplasmic reticulum stress Source: UniProtKBInferred from direct assayⁱ
  • Ref.7

    "CHOP potentially co-operates with FOXO3a in neuronal cells to regulate PUMA and BIM expression in response to ER stress."
    Ghosh A.P., Klocke B.J., Ballestas M.E., Roth K.A.
    PLoS ONE 7:E39586-E39586(2012) [PubMed] [Europe PMC] [Abstract]

    Cited for: INDUCTION.

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

Organism-specific databases

Miscellaneous databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityⁱ

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductionⁱ

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Motif

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4)ⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MARARQEGSS PEPVEGLARD GPRPFPLGRL VPSAVSCGLC EPGLAAAPAA 
        60         70         80         90        100
PTLLPAAYLC APTAPPAVTA ALGGSRWPGG PRSRPRGPRP DGPQPSLSLA 
       110        120        130        140        150
EQHLESPVPS APGALAGGPT QAAPGVRGEE EQWAREIGAQ LRRMADDLNA 
       160        170        180        190 
QYERRRQEEQ QRHRPSPWRV LYNLIMGLLP LPRGHRAPEM EPN        

        10         20         30         40         50
MKFGMGSAQA CPCQVPRAAS TTWVPCQICG PQPSLSLAEQ HLESPVPSAP 
        60         70         80         90        100
GALAGGPTQA APGVRGEEEQ WAREIGAQLR RMADDLNAQY ERRRQEEQQR 
       110        120        130 
HRPSPWRVLY NLIMGLLPLP RGHRAPEMEP N                     

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityⁱ

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families
3. Human chromosome 19
   Human chromosome 19: entries, gene names and cross-references to MIM
4. MIM cross-references
   Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot