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Protein

Testis-specific serine/threonine-protein kinase 1

Gene

TSSK1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.2 Publications

Miscellaneous

TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+By similarity

Activity regulationi

Kinase activity is specifically inhibited by 2 classes of compounds: biphenyl compounds (1,1'-(biphenyl-4,4'-diyl)bis(2,2-dihydroxyethanone)) and 1,2,7-trialky-1H-imidazo[4,5-g]quinoxalin-6-one. Activated by phosphorylation on Thr-174 and potentially by autophosphorylation.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Spermatogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ9BXA7

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 1 (EC:2.7.11.11 Publication)
Short name:
TSK-1
Short name:
TSK1
Short name:
TSSK-1
Short name:
Testis-specific kinase 1
Alternative name(s):
Serine/threonine-protein kinase 22A
Gene namesi
Name:TSSK1B
Synonyms:SPOGA1, SPOGA4, STK22A, STK22D, TSSK1
ORF Names:FKSG81
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000212122.3
HGNCiHGNC:14968 TSSK1B
MIMi610709 gene
neXtProtiNX_Q9BXA7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Flagellum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi174T → A: Loss of kinase activity. 1 Publication1
Mutagenesisi174T → E: Constitutively active. 1 Publication1

Organism-specific databases

DisGeNETi83942
OpenTargetsiENSG00000212122
PharmGKBiPA37944

Chemistry databases

ChEMBLiCHEMBL6003
GuidetoPHARMACOLOGYi2257

Polymorphism and mutation databases

BioMutaiTSSK1B
DMDMi30316282

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867661 – 367Testis-specific serine/threonine-protein kinase 1Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174Phosphothreonine1 Publication1

Post-translational modificationi

Autophosphorylated.1 Publication
Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9BXA7
PeptideAtlasiQ9BXA7
PRIDEiQ9BXA7
ProteomicsDBi79392

PTM databases

iPTMnetiQ9BXA7
PhosphoSitePlusiQ9BXA7

Expressioni

Tissue specificityi

Testis-specific. Present in sperm (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000212122 Expressed in 12 organ(s), highest expression level in right testis
CleanExiHS_TSSK1B
ExpressionAtlasiQ9BXA7 baseline and differential
GenevisibleiQ9BXA7 HS

Organism-specific databases

HPAiHPA027827

Interactioni

Subunit structurei

Interacts with TSSK2. Interacts with HSP90; this interaction stabilizes TSSK1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EFSO432813EBI-6423734,EBI-718488

Protein-protein interaction databases

BioGridi123825, 14 interactors
IntActiQ9BXA7, 6 interactors
STRINGi9606.ENSP00000375081

Chemistry databases

BindingDBiQ9BXA7

Structurei

3D structure databases

ProteinModelPortaliQ9BXA7
SMRiQ9BXA7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00770000120521
HOGENOMiHOG000233016
HOVERGENiHBG101110
InParanoidiQ9BXA7
KOiK08811
OMAiHIIQVHS
OrthoDBiEOG091G0EZI
PhylomeDBiQ9BXA7
TreeFamiTF352374

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q9BXA7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDAAVLKRR GYLLGINLGE GSYAKVKSAY SERLKFNVAI KIIDRKKAPA
60 70 80 90 100
DFLEKFLPRE IEILAMLNHC SIIKTYEIFE TSHGKVYIVM ELAVQGDLLE
110 120 130 140 150
LIKTRGALHE DEARKKFHQL SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI
160 170 180 190 200
KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA YAAPEVLQGI PYQPKVYDIW
210 220 230 240 250
SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY
260 270 280 290 300
HMLQPDVNRR LHIDEILSHC WMQPKARGSP SVAINKEGES SRGTEPLWTP
310 320 330 340 350
EPGSDKKSAT KLEPEGEAQP QAQPETKPEG TAMQMSRQSE ILGFPSKPST
360
METEEGPPQQ PPETRAQ
Length:367
Mass (Da):41,618
Last modified:June 1, 2001 - v1
Checksum:i1B740988894A1589
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti345P → L in BAG36136 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04123550A → T1 PublicationCorresponds to variant dbSNP:rs747955728Ensembl.1
Natural variantiVAR_04123683H → Y1 PublicationCorresponds to variant dbSNP:rs55930004Ensembl.1
Natural variantiVAR_041237233V → L1 PublicationCorresponds to variant dbSNP:rs55940513Ensembl.1
Natural variantiVAR_041238237R → C1 PublicationCorresponds to variant dbSNP:rs55738530Ensembl.1
Natural variantiVAR_041239288G → W1 PublicationCorresponds to variant dbSNP:rs34696815Ensembl.1
Natural variantiVAR_041240293G → E1 PublicationCorresponds to variant dbSNP:rs11953478Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028964 mRNA Translation: AAK27734.1
AF348076 mRNA Translation: AAK29413.1
AK313332 mRNA Translation: BAG36136.1
AC010431 Genomic DNA No translation available.
BC022515 mRNA Translation: AAH22515.1
CCDSiCCDS4112.1
RefSeqiNP_114417.1, NM_032028.3
UniGeneiHs.519507

Genome annotation databases

EnsembliENST00000390666; ENSP00000375081; ENSG00000212122
GeneIDi83942
KEGGihsa:83942
UCSCiuc003kqm.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028964 mRNA Translation: AAK27734.1
AF348076 mRNA Translation: AAK29413.1
AK313332 mRNA Translation: BAG36136.1
AC010431 Genomic DNA No translation available.
BC022515 mRNA Translation: AAH22515.1
CCDSiCCDS4112.1
RefSeqiNP_114417.1, NM_032028.3
UniGeneiHs.519507

3D structure databases

ProteinModelPortaliQ9BXA7
SMRiQ9BXA7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123825, 14 interactors
IntActiQ9BXA7, 6 interactors
STRINGi9606.ENSP00000375081

Chemistry databases

BindingDBiQ9BXA7
ChEMBLiCHEMBL6003
GuidetoPHARMACOLOGYi2257

PTM databases

iPTMnetiQ9BXA7
PhosphoSitePlusiQ9BXA7

Polymorphism and mutation databases

BioMutaiTSSK1B
DMDMi30316282

Proteomic databases

PaxDbiQ9BXA7
PeptideAtlasiQ9BXA7
PRIDEiQ9BXA7
ProteomicsDBi79392

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000390666; ENSP00000375081; ENSG00000212122
GeneIDi83942
KEGGihsa:83942
UCSCiuc003kqm.2 human

Organism-specific databases

CTDi83942
DisGeNETi83942
EuPathDBiHostDB:ENSG00000212122.3
GeneCardsiTSSK1B
HGNCiHGNC:14968 TSSK1B
HPAiHPA027827
MIMi610709 gene
neXtProtiNX_Q9BXA7
OpenTargetsiENSG00000212122
PharmGKBiPA37944
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00770000120521
HOGENOMiHOG000233016
HOVERGENiHBG101110
InParanoidiQ9BXA7
KOiK08811
OMAiHIIQVHS
OrthoDBiEOG091G0EZI
PhylomeDBiQ9BXA7
TreeFamiTF352374

Enzyme and pathway databases

SignaLinkiQ9BXA7

Miscellaneous databases

GenomeRNAii83942
PROiPR:Q9BXA7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000212122 Expressed in 12 organ(s), highest expression level in right testis
CleanExiHS_TSSK1B
ExpressionAtlasiQ9BXA7 baseline and differential
GenevisibleiQ9BXA7 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTSSK1_HUMAN
AccessioniPrimary (citable) accession number: Q9BXA7
Secondary accession number(s): B2R8D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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