TSSK6

Functionⁱ

Required for sperm production and function. Plays a role in DNA condensation during postmeiotic chromatin remodeling (By similarity).By similarity1 Publication

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactorⁱ

Mg²⁺1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	41	ATPPROSITE-ProRule annotation1 Publication		1
Active siteⁱ	135	Proton acceptorPROSITE-ProRule annotation1 Publication		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	18 – 26	ATPPROSITE-ProRule annotationBy similarity		9

GO - Molecular functionⁱ

ATP binding
Source: UniProtKB
Inferred from direct assayⁱ
- 15870294
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 15870294
protein-containing complex binding Source: Ensembl
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 15870294

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

intracellular signal transduction
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
multicellular organism development Source: UniProtKB-KW
peptidyl-serine phosphorylation Source: Ensembl
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 15870294
sperm chromatin condensation Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Differentiation, Spermatogenesis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.11.1 2681
SignaLinkⁱ	Q9BXA6

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Testis-specific serine/threonine-protein kinase 6 (EC:2.7.11.12 Publications) Short name: TSK-6 Short name: TSSK-6 Short name: Testis-specific kinase 6 Alternative name(s): Cancer/testis antigen 72 Short name: CT72 Serine/threonine-protein kinase SSTK Small serine/threonine kinase
Gene namesⁱ	Name:TSSK6Imported Synonyms:SSTKImported ORF Names:FKSG82
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000178093.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:30410 TSSK6
MIMⁱ	610712 gene
neXtProtⁱ	NX_Q9BXA6

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	41	K → M: Loss of kinase activity. Loss of binding to TSACC. 2 Publications		1
Mutagenesisⁱ	135	D → N: Loss of kinase activity. No effect of binding to TSACC. 2 Publications		1

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000178093
PharmGKBⁱ	PA142670682

Chemistry databases

ChEMBLⁱ	CHEMBL3627582

ChEMBLⁱ

CHEMBL3627582

Polymorphism and mutation databases

BioMutaⁱ	TSSK6
DMDMⁱ	74761315

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000227746	1 – 273	Testis-specific serine/threonine-protein kinase 6Add BLAST		273

Post-translational modificationⁱ

Autophosphorylated.1 Publication

Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.By similarity

Keywords - PTMⁱ

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	Q9BXA6
PeptideAtlas More...PeptideAtlasⁱ	Q9BXA6
PRIDEⁱ	Q9BXA6
ProteomicsDBⁱ	79391

PTM databases

PhosphoSitePlusⁱ	Q9BXA6

PhosphoSitePlusⁱ

Q9BXA6

Expressionⁱ

Tissue specificityⁱ

Highly expressed in testis. Expressed at lower levels in colon, small intestine, ovary, prostate, thymus, spleen and peripheral blood leukocytes.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000178093 Expressed in 92 organ(s), highest expression level in left testis
CleanExⁱ	HS_TSSK6
ExpressionAtlasⁱ	Q9BXA6 baseline and differential
Genevisibleⁱ	Q9BXA6 HS

Interactionⁱ

Subunit structureⁱ

Interacts with HSP90; this interaction stabilizes and activates TSSK6 (By similarity). Interacts with the heat shock proteins HSPCB, HSPA8 and HSPA1A. These interactions appear to be required for TSSK6 kinase activity. Interacts with TSACC; this interaction is direct and recruits TSACC to HSP90, which is essential for kinase activity.By similarity2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
HSP90AB1	P08238	3	EBI-851883,EBI-352572

With

Entry

#Exp.

IntAct

Notes

HSP90AB1

P08238

3

EBI-851883,EBI-352572

Protein-protein interaction databases

BioGridⁱ	123835, 22 interactors
IntActⁱ	Q9BXA6, 18 interactors
STRINGⁱ	9606.ENSP00000354168

Chemistry databases

BindingDBⁱ

Q9BXA6

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9BXA6
SMRⁱ	Q9BXA6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	12 – 267	Protein kinasePROSITE-ProRule annotationAdd BLAST		256

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0583 Eukaryota COG0515 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00770000120521
HOGENOMⁱ	HOG000233016
HOVERGENⁱ	HBG101110
InParanoidⁱ	Q9BXA6
KEGG Orthology (KO) More...KOⁱ	K08811
OMAⁱ	PCVPEAR
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0EZI
PhylomeDBⁱ	Q9BXA6
TreeFamⁱ	TF105333

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q9BXA6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP 
        60         70         80         90        100
DFVNKFLPRE LSILRGVRHP HIVHVFEFIE VCNGKLYIVM EAAATDLLQA 
       110        120        130        140        150
VQRNGRIPGV QARDLFAQIA GAVRYLHDHH LVHRDLKCEN VLLSPDERRV 
       160        170        180        190        200
KLTDFGFGRQ AHGYPDLSTT YCGSAAYASP EVLLGIPYDP KKYDVWSMGV 
       210        220        230        240        250
VLYVMVTGCM PFDDSDIAGL PRRQKRGVLY PEGLELSERC KALIAELLQF 
       260        270 
SPSARPSAGQ VARNCWLRAG DSG

Length:273

Mass (Da):30,331

Last modified:June 1, 2001 - v1

Checksum:ⁱ998934C72963C7CE

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	R4GMZ2	R4GMZ2_HUMAN		Testis-specific serine/threonine-pr... Testis-specific serine/threonine-protein kinase 6	TSSK6	109	Annotation score:

Sequence cautionⁱ

The sequence BAB71697 differs from that shown. Reason: Frameshift at positions 104 and 169.Curated

The sequence BAD18805 differs from that shown. Reason: Frameshift at position 247.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF329483 mRNA Translation: AAK48827.1 AF348077 mRNA Translation: AAK29414.1 AK058166 mRNA Translation: BAB71697.1 Frameshift. AK172841 mRNA Translation: BAD18805.1 Frameshift. AK313767 mRNA Translation: BAG36505.1 CH471106 Genomic DNA Translation: EAW84824.1 BC014611 mRNA Translation: AAH14611.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS12403.1
NCBI Reference Sequences More...RefSeqⁱ	NP_114426.1, NM_032037.3
UniGeneⁱ	Hs.532711 Hs.666780

Genome annotation databases

Ensemblⁱ	ENST00000585580; ENSP00000466477; ENSG00000178093 ENST00000587522; ENSP00000466056; ENSG00000178093
GeneIDⁱ	83983
KEGGⁱ	hsa:83983
UCSC genome browser More...UCSCⁱ	uc002nmq.4 human

Protein	Similar proteins		Species	Score	Length	Source
Q9BXA6	Testis secretory sperm-binding protein Li 205a		HUMAN		273	UniRef100_Q9BXA6
TSSK6 isoform 1		PONAB		273
TSSK6 isoform 3 (Fragment)		PANTR		109
Testis-specific serine/threonine-protein kinase 6 (Fragment)		HUMAN		109
TSSK6 isoform 3 (Fragment)		PONAB		109

Protein	Similar proteins		Species	Score	Length	Source
Q9BXA6	Testis-specific serine/threonine-protein kinase 6		MOUSE		273	UniRef90_Q9BXA6
Testis secretory sperm-binding protein Li 205a		HUMAN		273
TSSK6 isoform 1		PONAB		273
TSSK6 isoform 3 (Fragment)		PANTR		109
Uncharacterized protein		AILME		273
+89

Protein	Similar proteins		Species	Score	Length	Source
Q9BXA6	Testis-specific serine/threonine-protein kinase 6		MOUSE		273	UniRef50_Q9BXA6
Testis secretory sperm-binding protein Li 205a		HUMAN		273
TSSK6 isoform 1		PONAB		273
TSSK6 isoform 3 (Fragment)		PANTR		109
testis-specific serine/threonine-protein kinase 6		LEPWE		273
+166

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF329483 mRNA Translation: AAK48827.1 AF348077 mRNA Translation: AAK29414.1 AK058166 mRNA Translation: BAB71697.1 Frameshift. AK172841 mRNA Translation: BAD18805.1 Frameshift. AK313767 mRNA Translation: BAG36505.1 CH471106 Genomic DNA Translation: EAW84824.1 BC014611 mRNA Translation: AAH14611.1
CCDSⁱ	CCDS12403.1
RefSeqⁱ	NP_114426.1, NM_032037.3
UniGeneⁱ	Hs.532711 Hs.666780

3D structure databases

ProteinModelPortalⁱ	Q9BXA6
SMRⁱ	Q9BXA6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	123835, 22 interactors
IntActⁱ	Q9BXA6, 18 interactors
STRINGⁱ	9606.ENSP00000354168

Chemistry databases

BindingDBⁱ	Q9BXA6
ChEMBLⁱ	CHEMBL3627582

PTM databases

PhosphoSitePlusⁱ	Q9BXA6

PhosphoSitePlusⁱ

Q9BXA6

Polymorphism and mutation databases

BioMutaⁱ	TSSK6
DMDMⁱ	74761315

Proteomic databases

PaxDbⁱ	Q9BXA6
PeptideAtlasⁱ	Q9BXA6
PRIDEⁱ	Q9BXA6
ProteomicsDBⁱ	79391

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	83983
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000585580; ENSP00000466477; ENSG00000178093 ENST00000587522; ENSP00000466056; ENSG00000178093
GeneIDⁱ	83983
KEGGⁱ	hsa:83983
UCSCⁱ	uc002nmq.4 human

Organism-specific databases

CTDⁱ	83983
EuPathDBⁱ	HostDB:ENSG00000178093.13
GeneCardsⁱ	TSSK6
H-InvDBⁱ	HIX0174374
HGNCⁱ	HGNC:30410 TSSK6
MIMⁱ	610712 gene
neXtProtⁱ	NX_Q9BXA6
OpenTargetsⁱ	ENSG00000178093
PharmGKBⁱ	PA142670682
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0583 Eukaryota COG0515 LUCA
GeneTreeⁱ	ENSGT00770000120521
HOGENOMⁱ	HOG000233016
HOVERGENⁱ	HBG101110
InParanoidⁱ	Q9BXA6
KOⁱ	K08811
OMAⁱ	PCVPEAR
OrthoDBⁱ	EOG091G0EZI
PhylomeDBⁱ	Q9BXA6
TreeFamⁱ	TF105333

Enzyme and pathway databases

BRENDAⁱ	2.7.11.1 2681
SignaLinkⁱ	Q9BXA6

Miscellaneous databases

GenomeRNAiⁱ	83983
Protein Ontology More...PROⁱ	PR:Q9BXA6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000178093 Expressed in 92 organ(s), highest expression level in left testis
CleanExⁱ	HS_TSSK6
ExpressionAtlasⁱ	Q9BXA6 baseline and differential
Genevisibleⁱ	Q9BXA6 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfamⁱ	View protein in Pfam PF00069 Pkinase, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TSSK6_HUMAN
Accessionⁱ	Q9BXA6Primary (citable) accession number: Q9BXA6 Secondary accession number(s): B2R9F8, Q6ZMC4, Q96LJ4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
	Last sequence update:	June 1, 2001
	Last modified:	November 7, 2018
	This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM
Human and mouse protein kinases
Human and mouse protein kinases: classification and index

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UniProtKB - Q9BXA6 (TSSK6_HUMAN)

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Testis-specific serine/threonine-protein kinase 6

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ