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Protein

Histidine triad nucleotide-binding protein 2, mitochondrial

Gene

HINT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolase probably involved in steroid biosynthesis. May play a role in apoptosis. Has adenosine phosphoramidase activity.2 Publications

Kineticsi

  1. KM=128 µM for adenosine 5'-O-p-nitrophenylphosphoramidate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei63Purine nucleotide phosphoramidateCombined sources1 Publication1
    Binding sitei80Purine nucleotide phosphoramidateCombined sources1 Publication1
    Binding sitei136Purine nucleotide phosphoramidateCombined sources1 Publication1
    Active sitei149Tele-AMP-histidine intermediate1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi142 – 145Purine nucleotide phosphoramidateCombined sources1 Publication4
    Nucleotide bindingi149 – 151Purine nucleotide phosphoramidateCombined sources1 Publication3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processApoptosis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis
    LigandNucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histidine triad nucleotide-binding protein 2, mitochondrialCurated (EC:3.-.-.-)
    Short name:
    HINT-21 Publication
    Alternative name(s):
    HINT-31 Publication
    HIT-17kDa1 Publication
    PKCI-1-related HIT protein1 Publication
    Gene namesi
    Name:HINT2Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 9

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000137133.10
    HGNCiHGNC:18344 HINT2
    MIMi609997 gene
    neXtProtiNX_Q9BX68

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi149H → A: Loss of adenosine phosphoramidase activity. 1 Publication1

    Organism-specific databases

    DisGeNETi84681
    OpenTargetsiENSG00000137133
    PharmGKBiPA29287

    Polymorphism and mutation databases

    BioMutaiHINT2
    DMDMi51701612

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
    ChainiPRO_000010978618 – 163Histidine triad nucleotide-binding protein 2, mitochondrialAdd BLAST146

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei119N6-acetyllysineBy similarity1
    Modified residuei139N6-acetyllysineBy similarity1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    EPDiQ9BX68
    MaxQBiQ9BX68
    PaxDbiQ9BX68
    PeptideAtlasiQ9BX68
    PRIDEiQ9BX68
    ProteomicsDBi79368
    TopDownProteomicsiQ9BX68

    2D gel databases

    OGPiQ9BX68

    PTM databases

    iPTMnetiQ9BX68
    PhosphoSitePlusiQ9BX68

    Expressioni

    Tissue specificityi

    High expression in liver and pancreas. Expression is significantly down-regulated in hepatocellular carcinoma (HCC) patients.1 Publication

    Gene expression databases

    BgeeiENSG00000137133
    CleanExiHS_HINT2
    GenevisibleiQ9BX68 HS

    Organism-specific databases

    HPAiHPA020961
    HPA059109

    Interactioni

    Protein-protein interaction databases

    BioGridi124200, 33 interactors
    IntActiQ9BX68, 5 interactors
    MINTiQ9BX68
    STRINGi9606.ENSP00000259667

    Structurei

    Secondary structure

    1163
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi56 – 60Combined sources5
    Helixi65 – 67Combined sources3
    Beta strandi68 – 71Combined sources4
    Beta strandi73 – 79Combined sources7
    Beta strandi84 – 95Combined sources12
    Helixi100 – 102Combined sources3
    Helixi105 – 107Combined sources3
    Helixi108 – 124Combined sources17
    Beta strandi131 – 137Combined sources7
    Turni138 – 142Combined sources5
    Beta strandi145 – 147Combined sources3
    Beta strandi150 – 156Combined sources7

    3D structure databases

    ProteinModelPortaliQ9BX68
    SMRiQ9BX68
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini55 – 163HITPROSITE-ProRule annotationAdd BLAST109

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi147 – 151Histidine triad motif5

    Sequence similaritiesi

    Belongs to the HINT family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG3275 Eukaryota
    COG0537 LUCA
    GeneTreeiENSGT00510000046448
    HOGENOMiHOG000061064
    HOVERGENiHBG051906
    InParanoidiQ9BX68
    OMAiNVAKQES
    OrthoDBiEOG091G1001
    PhylomeDBiQ9BX68
    TreeFamiTF314862

    Family and domain databases

    Gene3Di3.30.428.10, 1 hit
    InterProiView protein in InterPro
    IPR019808 Histidine_triad_CS
    IPR001310 Histidine_triad_HIT
    IPR011146 HIT-like
    IPR036265 HIT-like_sf
    PANTHERiPTHR23089 PTHR23089, 1 hit
    PfamiView protein in Pfam
    PF01230 HIT, 1 hit
    PRINTSiPR00332 HISTRIAD
    SUPFAMiSSF54197 SSF54197, 1 hit
    PROSITEiView protein in PROSITE
    PS00892 HIT_1, 1 hit
    PS51084 HIT_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9BX68-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAAAVVLAAG LRAARRAVAA TGVRGGQVRG AAGVTDGNEV AKAQQATPGG
    60 70 80 90 100
    AAPTIFSRIL DKSLPADILY EDQQCLVFRD VAPQAPVHFL VIPKKPIPRI
    110 120 130 140 150
    SQAEEEDQQL LGHLLLVAKQ TAKAEGLGDG YRLVINDGKL GAQSVYHLHI
    160
    HVLGGRQLQW PPG
    Length:163
    Mass (Da):17,162
    Last modified:June 1, 2001 - v1
    Checksum:i63A0C15D13749F99
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF356515 mRNA Translation: AAK37562.1
    AY033094 mRNA Translation: AAK53455.1
    AF490476 Genomic DNA Translation: AAM09526.1
    AF356875 mRNA Translation: AAM00221.1
    AL133410 Genomic DNA No translation available.
    CH471071 Genomic DNA Translation: EAW58332.1
    BC047737 mRNA Translation: AAH47737.1
    CCDSiCCDS6594.1
    RefSeqiNP_115982.1, NM_032593.2
    UniGeneiHs.70573

    Genome annotation databases

    EnsembliENST00000259667; ENSP00000259667; ENSG00000137133
    GeneIDi84681
    KEGGihsa:84681
    UCSCiuc003zyh.4 human

    Similar proteinsi

    Entry informationi

    Entry nameiHINT2_HUMAN
    AccessioniPrimary (citable) accession number: Q9BX68
    Secondary accession number(s): Q5TCW3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: June 1, 2001
    Last modified: June 20, 2018
    This is version 117 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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