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Protein

Junctional adhesion molecule C

Gene

JAM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates cell-cell adhesion (PubMed:11590146, PubMed:12208882, PubMed:15194813). Functions as counter-receptor for JAM2 (PubMed:11590146). Functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (PubMed:12208882, PubMed:15194813). Plays a role in angiogenesis (PubMed:23255084). May play a role in the regulation of cell migration (Probable). Required for normal polarization and acrosome formation in developing spermatids, and for normal male fertility (By similarity).By similarity1 Publication4 Publications
Soluble form of JAM-C: Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.1 Publication

GO - Molecular functioni

  • integrin binding Source: UniProtKB
  • protein heterodimerization activity Source: Ensembl
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

Keywordsi

Biological processAngiogenesis, Cell adhesion, Differentiation, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-216083 Integrin cell surface interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Junctional adhesion molecule C
Short name:
JAM-C
Alternative name(s):
JAM-2
Junctional adhesion molecule 3
Short name:
JAM-3
Cleaved into the following chain:
Soluble form of JAM-C1 Publication
Short name:
sJAM-C1 Publication
Gene namesi
Name:JAM3
ORF Names:UNQ859/PRO1868
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000166086.12
HGNCiHGNC:15532 JAM3
MIMi606871 gene
neXtProtiNX_Q9BX67

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 241ExtracellularSequence analysisAdd BLAST210
Transmembranei242 – 262HelicalSequence analysisAdd BLAST21
Topological domaini263 – 310CytoplasmicSequence analysisAdd BLAST48

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted, Tight junction

Pathology & Biotechi

Involvement in diseasei

Hemorrhagic destruction of the brain with subependymal calcification and cataracts (HDBSCC)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by congenital cataracts and severe brain abnormalities apparently resulting from hemorrhagic destruction of the brain parenchyma, including the cerebral white matter and basal ganglia. Patients manifest profound developmental delay, and other neurologic features included seizures, spasticity, and hyperreflexia. The clinical course is very severe resulting in death in infancy. Brain imaging shows multifocal intraparenchymal hemorrhage with associated liquefaction and massive cystic degeneration, and calcification in the subependymal region and in brain tissue.
See also OMIM:613730
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069529116E → K in HDBSCC; normal location at the cell membrane. 1 PublicationCorresponds to variant dbSNP:rs397515439EnsemblClinVar.1
Natural variantiVAR_069530219C → Y in HDBSCC; the mutant is retained in the endoplasmic reticulum. 1 PublicationCorresponds to variant dbSNP:rs397515438EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi264C → S: Decreased palmitoylation. Abolishes palmitoylation; when associated with S-265. 1 Publication1
Mutagenesisi265C → S: Decreased palmitoylation. Abolishes palmitoylation; when associated with S-264. 1 Publication1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi83700
MalaCardsiJAM3
MIMi613730 phenotype
OpenTargetsiENSG00000166086
Orphaneti306547 Porencephaly-microcephaly-bilateral congenital cataract syndrome
PharmGKBiPA29993

Polymorphism and mutation databases

BioMutaiJAM3
DMDMi51701611

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 311 PublicationAdd BLAST31
ChainiPRO_000001507132 – 310Junctional adhesion molecule CAdd BLAST279
ChainiPRO_000044533632 – ?Soluble form of JAM-C

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 115PROSITE-ProRule annotation
Glycosylationi104N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi160 ↔ 219PROSITE-ProRule annotation
Glycosylationi192N-linked (GlcNAc...) asparagine1 Publication1
Lipidationi264S-palmitoyl cysteine1 Publication1
Lipidationi265S-palmitoyl cysteine1 Publication1

Post-translational modificationi

Proteolytically cleaved from endothelial cells surface into a soluble form by ADAM10 and ADAM17; the release of soluble JAM3 is increased by proinflammatory factors.1 Publication
S-palmitoylated by ZDHHC7. S-palmitoylation promotes expression at tight junctions.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

EPDiQ9BX67
PeptideAtlasiQ9BX67
PRIDEiQ9BX67
ProteomicsDBi79366
79367 [Q9BX67-2]

PTM databases

GlyConnecti672
iPTMnetiQ9BX67
PhosphoSitePlusiQ9BX67
SwissPalmiQ9BX67
UniCarbKBiQ9BX67

Expressioni

Tissue specificityi

Detected on round and elongated spermatids (at protein level) (PubMed:15372036). Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level).7 Publications

Gene expression databases

BgeeiENSG00000166086 Expressed in 234 organ(s), highest expression level in corpus callosum
CleanExiHS_JAM3
ExpressionAtlasiQ9BX67 baseline and differential
GenevisibleiQ9BX67 HS

Organism-specific databases

HPAiHPA003417
HPA050434

Interactioni

Subunit structurei

Interacts with GORASP2 (By similarity). Interacts with JAM2 (PubMed:11590146). Interacts with ITGAM (PubMed:12208882, PubMed:15194813).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
JAM2P570872EBI-4314733,EBI-3918416

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123734, 7 interactors
IntActiQ9BX67, 3 interactors
MINTiQ9BX67

Structurei

3D structure databases

ProteinModelPortaliQ9BX67
SMRiQ9BX67
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 127Ig-like V-typeAdd BLAST93
Domaini139 – 236Ig-like C2-typeAdd BLAST98

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000110678
HOGENOMiHOG000247041
HOVERGENiHBG000518
InParanoidiQ9BX67
KOiK06785
OMAiQTNDPRI
OrthoDBiEOG091G0DWZ
PhylomeDBiQ9BX67
TreeFamiTF331459

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SM00408 IGc2, 2 hits
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BX67-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALRRPPRLR LCARLPDFFL LLLFRGCLIG AVNLKSSNRT PVVQEFESVE
60 70 80 90 100
LSCIITDSQT SDPRIEWKKI QDEQTTYVFF DNKIQGDLAG RAEILGKTSL
110 120 130 140 150
KIWNVTRRDS ALYRCEVVAR NDRKEIDEIV IELTVQVKPV TPVCRVPKAV
160 170 180 190 200
PVGKMATLHC QESEGHPRPH YSWYRNDVPL PTDSRANPRF RNSSFHLNSE
210 220 230 240 250
TGTLVFTAVH KDDSGQYYCI ASNDAGSARC EEQEMEVYDL NIGGIIGGVL
260 270 280 290 300
VVLAVLALIT LGICCAYRRG YFINNKQDGE SYKNPGKPDG VNYIRTDEEG
310
DFRHKSSFVI
Length:310
Mass (Da):35,020
Last modified:June 1, 2001 - v1
Checksum:iCE39ADF33EA1DAB9
GO
Isoform 2 (identifier: Q9BX67-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     85-135: Missing.

Note: No experimental confirmation available.
Show »
Length:259
Mass (Da):29,223
Checksum:i00F852424B415045
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YD98H0YD98_HUMAN
Junctional adhesion molecule C
JAM3
124Annotation score:
H0YCW9H0YCW9_HUMAN
Junctional adhesion molecule C
JAM3
38Annotation score:

Sequence cautioni

The sequence CAC94776 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136Q → R in AAH10690 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069529116E → K in HDBSCC; normal location at the cell membrane. 1 PublicationCorresponds to variant dbSNP:rs397515439EnsemblClinVar.1
Natural variantiVAR_069530219C → Y in HDBSCC; the mutant is retained in the endoplasmic reticulum. 1 PublicationCorresponds to variant dbSNP:rs397515438EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04256185 – 135Missing in isoform 2. 1 PublicationAdd BLAST51

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356518 mRNA Translation: AAK27221.1
AJ344431 mRNA Translation: CAC69845.1
AF448478 mRNA Translation: AAM20925.1
AJ416101 mRNA Translation: CAC94776.1 Different initiation.
AK074769 mRNA Translation: BAC11195.1
AK075309 mRNA Translation: BAC11538.1
AK125071 mRNA Translation: BAG54131.1
AY358335 mRNA Translation: AAQ88701.1
AP000911 Genomic DNA No translation available.
AP001775 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67820.1
BC010690 mRNA Translation: AAH10690.1
BC012147 mRNA Translation: AAH12147.1
CCDSiCCDS55799.1 [Q9BX67-2]
CCDS8494.2 [Q9BX67-1]
RefSeqiNP_001192258.1, NM_001205329.1 [Q9BX67-2]
NP_116190.3, NM_032801.4 [Q9BX67-1]
UniGeneiHs.150718

Genome annotation databases

EnsembliENST00000299106; ENSP00000299106; ENSG00000166086 [Q9BX67-1]
ENST00000441717; ENSP00000395742; ENSG00000166086 [Q9BX67-2]
GeneIDi83700
KEGGihsa:83700
UCSCiuc001qhb.4 human [Q9BX67-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF356518 mRNA Translation: AAK27221.1
AJ344431 mRNA Translation: CAC69845.1
AF448478 mRNA Translation: AAM20925.1
AJ416101 mRNA Translation: CAC94776.1 Different initiation.
AK074769 mRNA Translation: BAC11195.1
AK075309 mRNA Translation: BAC11538.1
AK125071 mRNA Translation: BAG54131.1
AY358335 mRNA Translation: AAQ88701.1
AP000911 Genomic DNA No translation available.
AP001775 Genomic DNA No translation available.
CH471065 Genomic DNA Translation: EAW67820.1
BC010690 mRNA Translation: AAH10690.1
BC012147 mRNA Translation: AAH12147.1
CCDSiCCDS55799.1 [Q9BX67-2]
CCDS8494.2 [Q9BX67-1]
RefSeqiNP_001192258.1, NM_001205329.1 [Q9BX67-2]
NP_116190.3, NM_032801.4 [Q9BX67-1]
UniGeneiHs.150718

3D structure databases

ProteinModelPortaliQ9BX67
SMRiQ9BX67
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123734, 7 interactors
IntActiQ9BX67, 3 interactors
MINTiQ9BX67

PTM databases

GlyConnecti672
iPTMnetiQ9BX67
PhosphoSitePlusiQ9BX67
SwissPalmiQ9BX67
UniCarbKBiQ9BX67

Polymorphism and mutation databases

BioMutaiJAM3
DMDMi51701611

Proteomic databases

EPDiQ9BX67
PeptideAtlasiQ9BX67
PRIDEiQ9BX67
ProteomicsDBi79366
79367 [Q9BX67-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299106; ENSP00000299106; ENSG00000166086 [Q9BX67-1]
ENST00000441717; ENSP00000395742; ENSG00000166086 [Q9BX67-2]
GeneIDi83700
KEGGihsa:83700
UCSCiuc001qhb.4 human [Q9BX67-1]

Organism-specific databases

CTDi83700
DisGeNETi83700
EuPathDBiHostDB:ENSG00000166086.12
GeneCardsiJAM3
HGNCiHGNC:15532 JAM3
HPAiHPA003417
HPA050434
MalaCardsiJAM3
MIMi606871 gene
613730 phenotype
neXtProtiNX_Q9BX67
OpenTargetsiENSG00000166086
Orphaneti306547 Porencephaly-microcephaly-bilateral congenital cataract syndrome
PharmGKBiPA29993
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00730000110678
HOGENOMiHOG000247041
HOVERGENiHBG000518
InParanoidiQ9BX67
KOiK06785
OMAiQTNDPRI
OrthoDBiEOG091G0DWZ
PhylomeDBiQ9BX67
TreeFamiTF331459

Enzyme and pathway databases

ReactomeiR-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-216083 Integrin cell surface interactions

Miscellaneous databases

ChiTaRSiJAM3 human
GeneWikiiJAM3
GenomeRNAii83700
PROiPR:Q9BX67
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166086 Expressed in 234 organ(s), highest expression level in corpus callosum
CleanExiHS_JAM3
ExpressionAtlasiQ9BX67 baseline and differential
GenevisibleiQ9BX67 HS

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SM00408 IGc2, 2 hits
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiJAM3_HUMAN
AccessioniPrimary (citable) accession number: Q9BX67
Secondary accession number(s): B3KWG9, Q8WWL8, Q96FL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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