UniProtKB - Q9BW11 (MAD3_HUMAN)
Protein
Max dimerization protein 3
Gene
MXD3
Organism
Homo sapiens (Human)
Status
Functioni
Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation (By similarity).By similarity
GO - Molecular functioni
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9BW11 |
SIGNORi | Q9BW11 |
Names & Taxonomyi
Protein namesi | Recommended name: Max dimerization protein 3Short name: Max dimerizer 3 Alternative name(s): Class C basic helix-loop-helix protein 13 Short name: bHLHc13 Max-associated protein 3 Max-interacting transcriptional repressor MAD3 Myx |
Gene namesi | Name:MXD3 Synonyms:BHLHC13, MAD3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14008, MXD3 |
MIMi | 609450, gene |
neXtProti | NX_Q9BW11 |
VEuPathDBi | HostDB:ENSG00000213347.10 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 83463 |
OpenTargetsi | ENSG00000213347 |
PharmGKBi | PA134892226 |
Miscellaneous databases
Pharosi | Q9BW11, Tbio |
Genetic variation databases
BioMutai | MXD3 |
DMDMi | 74733390 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000253707 | 1 – 206 | Max dimerization protein 3Add BLAST | 206 |
Proteomic databases
EPDi | Q9BW11 |
jPOSTi | Q9BW11 |
MassIVEi | Q9BW11 |
PaxDbi | Q9BW11 |
PeptideAtlasi | Q9BW11 |
PRIDEi | Q9BW11 |
ProteomicsDBi | 5676 79247 [Q9BW11-1] 79248 [Q9BW11-2] 79249 [Q9BW11-3] |
PTM databases
iPTMneti | Q9BW11 |
PhosphoSitePlusi | Q9BW11 |
Expressioni
Gene expression databases
Bgeei | ENSG00000213347, Expressed in blood and 187 other tissues |
ExpressionAtlasi | Q9BW11, baseline and differential |
Genevisiblei | Q9BW11, HS |
Organism-specific databases
HPAi | ENSG00000213347, Tissue enhanced (bone marrow, lymphoid tissue) |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX.
Interacts with SIN3A AND SIN3B.
Interacts with RNF17 (By similarity).
By similarityBinary interactionsi
Hide detailsQ9BW11
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 123658, 36 interactors |
IntActi | Q9BW11, 31 interactors |
STRINGi | 9606.ENSP00000401867 |
Miscellaneous databases
RNActi | Q9BW11, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 109 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 8 – 25 | Interaction with SIN3A and SIN3BBy similarityAdd BLAST | 18 |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000161050 |
HOGENOMi | CLU_882682_0_0_1 |
InParanoidi | Q9BW11 |
OMAi | LYSCQAH |
PhylomeDBi | Q9BW11 |
TreeFami | TF315654 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9BW11-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEPLASNIQV LLQAAEFLER REREAEHGYA SLCPHRSPGP IHRRKKRPPQ
60 70 80 90 100
APGAQDSGRS VHNELEKRRR AQLKRCLERL KQQMPLGADC ARYTTLSLLR
110 120 130 140 150
RARMHIQKLE DQEQRARQLK ERLRSKQQSL QRQLEQLRGL AGAAERERLR
160 170 180 190 200
ADSLDSSGLS SERSDSDQEE LEVDVESLVF GGEAELLRGF VAGQEHSYSH
GGGAWL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RED4 | D6RED4_HUMAN | Max dimerization protein 3 | MXD3 | 124 | Annotation score: | ||
D6RD21 | D6RD21_HUMAN | Max dimerization protein 3 | MXD3 | 74 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | A → G in AAP97233 (Ref. 1) Curated | 1 | |
Sequence conflicti | 129 | S → G in BAD96299 (Ref. 3) Curated | 1 | |
Sequence conflicti | 134 – 135 | LE → WM in AAP97233 (Ref. 1) Curated | 2 | |
Sequence conflicti | 155 | D → N in AAP97233 (Ref. 1) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049546 | 114 | Q → H. Corresponds to variant dbSNP:rs35691394Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021109 | 60 – 68 | Missing in isoform 2. 1 Publication | 9 | |
Alternative sequenceiVSP_021110 | 169 – 206 | EELEV…GGAWL → VLPNENGGTPNHRPTGRGNN ISSHH in isoform 3. 1 PublicationAdd BLAST | 38 | |
Alternative sequenceiVSP_057220 | 169 – 206 | EELEV…GGAWL → GECPEAEGLCGPRGEPGLLT GPLPAQRSWRWMWRAWCLGV RPSCCGASSPARSTATRTAA APGYDVPHPGRASALYSCQA HLPGRSPPQAFRAARSHLLE WTKRTLVWEQVLPKHPAAGC QAGPLEGKGQDSSGPPWTPA GQAAWARAQAFGSAAPKGTG SLLGLFPSWTRPPAFASHKL YSIFIKSLFHNFPSIVLPNE NGGTPNHRPTGRGNNISSHH in isoform 4. 1 PublicationAdd BLAST | 38 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF114834 mRNA Translation: AAP97233.1 AK057034 mRNA Translation: BAB71352.1 AK303397 mRNA Translation: BAG64453.1 AK316468 mRNA Translation: BAH14839.1 AK222579 mRNA Translation: BAD96299.1 AL833959 mRNA Translation: CAD38809.2 AC146507 Genomic DNA No translation available. BC000745 mRNA Translation: AAH00745.1 |
CCDSi | CCDS4416.1 [Q9BW11-1] CCDS47347.1 [Q9BW11-3] |
RefSeqi | NP_001136407.1, NM_001142935.1 [Q9BW11-3] NP_112590.1, NM_031300.3 [Q9BW11-1] |
Genome annotation databases
Ensembli | ENST00000423571; ENSP00000389716; ENSG00000213347 [Q9BW11-4] ENST00000427908; ENSP00000416921; ENSG00000213347 [Q9BW11-3] ENST00000439742; ENSP00000401867; ENSG00000213347 [Q9BW11-1] ENST00000513063; ENSP00000421463; ENSG00000213347 [Q9BW11-1] |
GeneIDi | 83463 |
KEGGi | hsa:83463 |
UCSCi | uc003mga.4, human [Q9BW11-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF114834 mRNA Translation: AAP97233.1 AK057034 mRNA Translation: BAB71352.1 AK303397 mRNA Translation: BAG64453.1 AK316468 mRNA Translation: BAH14839.1 AK222579 mRNA Translation: BAD96299.1 AL833959 mRNA Translation: CAD38809.2 AC146507 Genomic DNA No translation available. BC000745 mRNA Translation: AAH00745.1 |
CCDSi | CCDS4416.1 [Q9BW11-1] CCDS47347.1 [Q9BW11-3] |
RefSeqi | NP_001136407.1, NM_001142935.1 [Q9BW11-3] NP_112590.1, NM_031300.3 [Q9BW11-1] |
3D structure databases
SMRi | Q9BW11 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123658, 36 interactors |
IntActi | Q9BW11, 31 interactors |
STRINGi | 9606.ENSP00000401867 |
PTM databases
iPTMneti | Q9BW11 |
PhosphoSitePlusi | Q9BW11 |
Genetic variation databases
BioMutai | MXD3 |
DMDMi | 74733390 |
Proteomic databases
EPDi | Q9BW11 |
jPOSTi | Q9BW11 |
MassIVEi | Q9BW11 |
PaxDbi | Q9BW11 |
PeptideAtlasi | Q9BW11 |
PRIDEi | Q9BW11 |
ProteomicsDBi | 5676 79247 [Q9BW11-1] 79248 [Q9BW11-2] 79249 [Q9BW11-3] |
Protocols and materials databases
ABCDi | Q9BW11, 1 sequenced antibody |
Antibodypediai | 17372, 231 antibodies |
DNASUi | 83463 |
Genome annotation databases
Ensembli | ENST00000423571; ENSP00000389716; ENSG00000213347 [Q9BW11-4] ENST00000427908; ENSP00000416921; ENSG00000213347 [Q9BW11-3] ENST00000439742; ENSP00000401867; ENSG00000213347 [Q9BW11-1] ENST00000513063; ENSP00000421463; ENSG00000213347 [Q9BW11-1] |
GeneIDi | 83463 |
KEGGi | hsa:83463 |
UCSCi | uc003mga.4, human [Q9BW11-1] |
Organism-specific databases
CTDi | 83463 |
DisGeNETi | 83463 |
GeneCardsi | MXD3 |
HGNCi | HGNC:14008, MXD3 |
HPAi | ENSG00000213347, Tissue enhanced (bone marrow, lymphoid tissue) |
MIMi | 609450, gene |
neXtProti | NX_Q9BW11 |
OpenTargetsi | ENSG00000213347 |
PharmGKBi | PA134892226 |
VEuPathDBi | HostDB:ENSG00000213347.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2483, Eukaryota |
GeneTreei | ENSGT00940000161050 |
HOGENOMi | CLU_882682_0_0_1 |
InParanoidi | Q9BW11 |
OMAi | LYSCQAH |
PhylomeDBi | Q9BW11 |
TreeFami | TF315654 |
Enzyme and pathway databases
PathwayCommonsi | Q9BW11 |
SIGNORi | Q9BW11 |
Miscellaneous databases
BioGRID-ORCSi | 83463, 6 hits in 1013 CRISPR screens |
ChiTaRSi | MXD3, human |
GenomeRNAii | 83463 |
Pharosi | Q9BW11, Tbio |
PROi | PR:Q9BW11 |
RNActi | Q9BW11, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000213347, Expressed in blood and 187 other tissues |
ExpressionAtlasi | Q9BW11, baseline and differential |
Genevisiblei | Q9BW11, HS |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAD3_HUMAN | |
Accessioni | Q9BW11Primary (citable) accession number: Q9BW11 Secondary accession number(s): B4E0J1 Q96ME3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot