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Protein

Serine/threonine-protein kinase RIO2

Gene

RIOK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed:19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed:21880710).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123ATPBy similarity1
Active sitei228Proton acceptorBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: GO_Central
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Ribosome biogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RIO2 (EC:2.7.11.12 Publications)
Alternative name(s):
RIO kinase 2
Gene namesi
Name:RIOK2Imported
Synonyms:RIO21 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000058729.10
HGNCiHGNC:18999 RIOK2
MIMi617754 gene
neXtProtiNX_Q9BVS4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi123K → A: Abolishes autophosphorylation; impairs release of pre-40S trans-acting factors and rRNA processing; when associated with A-246. 1 Publication1
Mutagenesisi246D → A: Abolishes autophosphorylation; impairs release of pre-40S trans-acting factors and rRNA processing; when associated with A-123. 1 Publication1
Mutagenesisi335S → A: Does not affect autophosphorylation activity; when associated with A-380 and A-548. Does not affect the timing of metaphase-anaphase transition; when associated with A-380 and A-548. 1 Publication1
Mutagenesisi335S → D: Increases time spent in metaphase; when associated with D-380 and D-548. 1 Publication1
Mutagenesisi380S → A: Does not affect autophosphorylation activity; when associated with A-335 and A-548. Does not affect the timing of metaphase-anaphase transition; when associated with A-335 and A-548. 1 Publication1
Mutagenesisi380S → D: Increases time spent in metaphase; when associated with D-335 and D-548. 1 Publication1
Mutagenesisi400L → A: Nuclear relocalization; when associated with A-403. 1 Publication1
Mutagenesisi403I → A: Nuclear relocalization; when associated with A-400. 1 Publication1
Mutagenesisi548S → A: Does not affect autophosphorylation activity; when associated with A-335 and A-380. Does not affect the timing of metaphase-anaphase transition; when associated with A-335 and A-380. 1 Publication1
Mutagenesisi548S → D: Increases time spent in metaphase; when associated with D-335 and D-380. 1 Publication1

Organism-specific databases

DisGeNETi55781
OpenTargetsiENSG00000058729
PharmGKBiPA134885533

Chemistry databases

ChEMBLiCHEMBL6000

Polymorphism and mutation databases

BioMutaiRIOK2
DMDMi143811448

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135271 – 552Serine/threonine-protein kinase RIO2Add BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei332PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1 Publication1
Modified residuei337PhosphoserineCombined sources1
Modified residuei350PhosphoserineCombined sources1
Modified residuei362PhosphoserineCombined sources1
Modified residuei380PhosphoserineCombined sources1 Publication1
Modified residuei382PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1
Modified residuei390PhosphoserineCombined sources1
Modified residuei412PhosphoserineCombined sources1
Modified residuei417PhosphoserineCombined sources1
Modified residuei442PhosphoserineCombined sources1
Modified residuei445PhosphotyrosineCombined sources1
Modified residuei548Phosphoserine1 Publication1

Post-translational modificationi

Autophosphorylated (in vitro) (PubMed:19564402, PubMed:21880710). Phosphorylation at Ser-335, Ser-380, Ser-548 by PLK1 affects the timing of the metaphase-anaphase transition (PubMed:21880710).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BVS4
MaxQBiQ9BVS4
PaxDbiQ9BVS4
PeptideAtlasiQ9BVS4
PRIDEiQ9BVS4
ProteomicsDBi79230

PTM databases

iPTMnetiQ9BVS4
PhosphoSitePlusiQ9BVS4

Expressioni

Gene expression databases

BgeeiENSG00000058729 Expressed in 208 organ(s), highest expression level in oocyte
CleanExiHS_RIOK2
ExpressionAtlasiQ9BVS4 baseline and differential
GenevisibleiQ9BVS4 HS

Organism-specific databases

HPAiHPA005681

Interactioni

Subunit structurei

Associated with late 40S pre-ribosomal particles (PubMed:16037817, PubMed:21081503). Interacts with PLK1 (via its N-terminus) (PubMed:21880710).2 Publications

Protein-protein interaction databases

BioGridi120896, 31 interactors
IntActiQ9BVS4, 24 interactors
STRINGi9606.ENSP00000283109

Chemistry databases

BindingDBiQ9BVS4

Structurei

Secondary structure

1552
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BVS4
SMRiQ9BVS4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 272Protein kinaseCuratedAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi399 – 408Nuclear export signal1 Publication10

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2268 Eukaryota
COG0478 LUCA
GeneTreeiENSGT00390000003255
HOGENOMiHOG000225685
HOVERGENiHBG056045
InParanoidiQ9BVS4
KOiK07179
OMAiVDYNRHA
OrthoDBiEOG091G06ON
PhylomeDBiQ9BVS4
TreeFamiTF321400

Family and domain databases

CDDicd05144 RIO2_C, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR030484 Rio2
IPR015285 RIO2_wHTH_N
IPR000687 RIO_kinase
IPR018935 RIO_kinase_CS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF09202 Rio2_N, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01245 RIO1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BVS4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKVNVAKLR YMSRDDFRVL TAVEMGMKNH EIVPGSLIAS IASLKHGGCN
60 70 80 90 100
KVLRELVKHK LIAWERTKTV QGYRLTNAGY DYLALKTLSS RQVVESVGNQ
110 120 130 140 150
MGVGKESDIY IVANEEGQQF ALKLHRLGRT SFRNLKNKRD YHKHRHNVSW
160 170 180 190 200
LYLSRLSAMK EFAYMKALYE RKFPVPKPID YNRHAVVMEL INGYPLCQIH
210 220 230 240 250
HVEDPASVYD EAMELIVKLA NHGLIHGDFN EFNLILDESD HITMIDFPQM
260 270 280 290 300
VSTSHPNAEW YFDRDVKCIK DFFMKRFSYE SELFPTFKDI RREDTLDVEV
310 320 330 340 350
SASGYTKEMQ ADDELLHPLG PDDKNIETKE GSEFSFSDGE VAEKAEVYGS
360 370 380 390 400
ENESERNCLE ESEGCYCRSS GDPEQIKEDS LSEESADARS FEMTEFNQAL
410 420 430 440 450
EEIKGQVVEN NSVTEFSEEK NRTENYNRQD GQRVQGGVPA GSDEYEDECP
460 470 480 490 500
HLIALSSLNR EFRPFRDEEN VGAMNQYRTR TLSITSSGSA VSCSTIPPEL
510 520 530 540 550
VKQKVKRQLT KQQKSAVRRR LQKGEANIFT KQRRENMQNI KSSLEAASFW

GE
Length:552
Mass (Da):63,283
Last modified:April 3, 2007 - v2
Checksum:i6EB260E72DDB78D7
GO
Isoform 2 (identifier: Q9BVS4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     467-552: DEENVGAMNQ...SLEAASFWGE → YRLLSIAF

Note: No experimental confirmation available.
Show »
Length:474
Mass (Da):54,508
Checksum:i4B396021BE81BE84
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y919H0Y919_HUMAN
Serine/threonine-protein kinase RIO...
RIOK2
140Annotation score:
H0Y8R4H0Y8R4_HUMAN
Serine/threonine-protein kinase RIO...
RIOK2
159Annotation score:
D6RB96D6RB96_HUMAN
Serine/threonine-protein kinase RIO...
RIOK2
37Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04234796S → C1 PublicationCorresponds to variant dbSNP:rs2544773Ensembl.1
Natural variantiVAR_042348144H → R1 PublicationCorresponds to variant dbSNP:rs35165987Ensembl.1
Natural variantiVAR_031597144H → Y1 PublicationCorresponds to variant dbSNP:rs17849382Ensembl.1
Natural variantiVAR_042349155R → H1 PublicationCorresponds to variant dbSNP:rs34916955Ensembl.1
Natural variantiVAR_042350175V → I1 PublicationCorresponds to variant dbSNP:rs35713904Ensembl.1
Natural variantiVAR_042351216I → T in a renal clear cell carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs147608663Ensembl.1
Natural variantiVAR_042352244M → V1 PublicationCorresponds to variant dbSNP:rs33996030Ensembl.1
Natural variantiVAR_031598349G → R2 PublicationsCorresponds to variant dbSNP:rs160632Ensembl.1
Natural variantiVAR_031599397N → S1 PublicationCorresponds to variant dbSNP:rs12188395Ensembl.1
Natural variantiVAR_042353409E → D1 PublicationCorresponds to variant dbSNP:rs35829000Ensembl.1
Natural variantiVAR_042354507R → H1 PublicationCorresponds to variant dbSNP:rs34555783Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046388467 – 552DEENV…SFWGE → YRLLSIAF in isoform 2. 1 PublicationAdd BLAST86

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002021 mRNA Translation: BAA92040.1
AK225348 mRNA No translation available.
AC008865 Genomic DNA No translation available.
AC008883 Genomic DNA No translation available.
BC000953 mRNA Translation: AAH00953.1
CCDSiCCDS4089.1 [Q9BVS4-1]
CCDS54884.1 [Q9BVS4-2]
RefSeqiNP_001153221.1, NM_001159749.1 [Q9BVS4-2]
NP_060813.2, NM_018343.2 [Q9BVS4-1]
UniGeneiHs.27021

Genome annotation databases

EnsembliENST00000283109; ENSP00000283109; ENSG00000058729 [Q9BVS4-1]
ENST00000508447; ENSP00000420932; ENSG00000058729 [Q9BVS4-2]
GeneIDi55781
KEGGihsa:55781
UCSCiuc003kmz.4 human [Q9BVS4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002021 mRNA Translation: BAA92040.1
AK225348 mRNA No translation available.
AC008865 Genomic DNA No translation available.
AC008883 Genomic DNA No translation available.
BC000953 mRNA Translation: AAH00953.1
CCDSiCCDS4089.1 [Q9BVS4-1]
CCDS54884.1 [Q9BVS4-2]
RefSeqiNP_001153221.1, NM_001159749.1 [Q9BVS4-2]
NP_060813.2, NM_018343.2 [Q9BVS4-1]
UniGeneiHs.27021

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DHFX-ray2.29D389-403[»]
6G18electron microscopy3.60v1-552[»]
6G51electron microscopy4.10v1-552[»]
ProteinModelPortaliQ9BVS4
SMRiQ9BVS4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120896, 31 interactors
IntActiQ9BVS4, 24 interactors
STRINGi9606.ENSP00000283109

Chemistry databases

BindingDBiQ9BVS4
ChEMBLiCHEMBL6000

PTM databases

iPTMnetiQ9BVS4
PhosphoSitePlusiQ9BVS4

Polymorphism and mutation databases

BioMutaiRIOK2
DMDMi143811448

Proteomic databases

EPDiQ9BVS4
MaxQBiQ9BVS4
PaxDbiQ9BVS4
PeptideAtlasiQ9BVS4
PRIDEiQ9BVS4
ProteomicsDBi79230

Protocols and materials databases

DNASUi55781
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000283109; ENSP00000283109; ENSG00000058729 [Q9BVS4-1]
ENST00000508447; ENSP00000420932; ENSG00000058729 [Q9BVS4-2]
GeneIDi55781
KEGGihsa:55781
UCSCiuc003kmz.4 human [Q9BVS4-1]

Organism-specific databases

CTDi55781
DisGeNETi55781
EuPathDBiHostDB:ENSG00000058729.10
GeneCardsiRIOK2
HGNCiHGNC:18999 RIOK2
HPAiHPA005681
MIMi617754 gene
neXtProtiNX_Q9BVS4
OpenTargetsiENSG00000058729
PharmGKBiPA134885533
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2268 Eukaryota
COG0478 LUCA
GeneTreeiENSGT00390000003255
HOGENOMiHOG000225685
HOVERGENiHBG056045
InParanoidiQ9BVS4
KOiK07179
OMAiVDYNRHA
OrthoDBiEOG091G06ON
PhylomeDBiQ9BVS4
TreeFamiTF321400

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

GenomeRNAii55781
PROiPR:Q9BVS4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000058729 Expressed in 208 organ(s), highest expression level in oocyte
CleanExiHS_RIOK2
ExpressionAtlasiQ9BVS4 baseline and differential
GenevisibleiQ9BVS4 HS

Family and domain databases

CDDicd05144 RIO2_C, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR030484 Rio2
IPR015285 RIO2_wHTH_N
IPR000687 RIO_kinase
IPR018935 RIO_kinase_CS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF09202 Rio2_N, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01245 RIO1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIOK2_HUMAN
AccessioniPrimary (citable) accession number: Q9BVS4
Secondary accession number(s): D6RDI3, Q9NUT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: April 3, 2007
Last modified: November 7, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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