UniProtKB - Q9BV86 (NTM1A_HUMAN)
Protein
N-terminal Xaa-Pro-Lys N-methyltransferase 1
Gene
NTMT1
Organism
Homo sapiens (Human)
Status
Functioni
Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation (PubMed:24090352). Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.4 Publications
Catalytic activityi
- N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H+ + N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.2442 Publications
- N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H+ + N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.2442 Publications
- N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = 2 H+ + N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine2 PublicationsEC:2.1.1.2442 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 69 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources2 Publications | 1 | |
Binding sitei | 74 | S-adenosyl-L-methionineCombined sources2 Publications | 1 | |
Binding sitei | 135 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources2 Publications | 1 |
GO - Molecular functioni
- histone methyltransferase activity Source: UniProtKB
- methyltransferase activity Source: GO_Central
- N-terminal protein N-methyltransferase activity Source: UniProtKB
- protein methyltransferase activity Source: UniProtKB
GO - Biological processi
- chromosome segregation Source: UniProtKB
- histone methylation Source: UniProtKB
- N-terminal peptidyl-alanine methylation Source: GO_Central
- N-terminal peptidyl-alanine trimethylation Source: Ensembl
- N-terminal peptidyl-glycine methylation Source: UniProtKB
- N-terminal peptidyl-proline dimethylation Source: UniProtKB
- N-terminal peptidyl-proline methylation Source: GO_Central
- N-terminal peptidyl-serine dimethylation Source: UniProtKB
- N-terminal peptidyl-serine methylation Source: GO_Central
- N-terminal peptidyl-serine trimethylation Source: UniProtKB
- N-terminal protein amino acid methylation Source: GO_Central
- spindle organization Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000148335-MONOMER |
BRENDAi | 2.1.1.244, 2681 |
PathwayCommonsi | Q9BV86 |
Names & Taxonomyi
Protein namesi | Recommended name: N-terminal Xaa-Pro-Lys N-methyltransferase 1 (EC:2.1.1.2441 Publication)Alternative name(s): Alpha N-terminal protein methyltransferase 1A Methyltransferase-like protein 11A N-terminal RCC1 methyltransferase X-Pro-Lys N-terminal protein methyltransferase 1A Short name: NTM1A Cleaved into the following chain: |
Gene namesi | Name:NTMT1 Synonyms:C9orf32, METTL11A, NRMT, NRMT1 ORF Names:AD-003 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000148335.14 |
HGNCi | HGNC:23373, NTMT1 |
MIMi | 613560, gene |
neXtProti | NX_Q9BV86 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 19 | Y → A or F: Decreased methyltransferase activity with CENPA. 1 Publication | 1 | |
Mutagenesisi | 19 | Y → A: Reduced methyltransferase activity with CENPA. 1 Publication | 1 | |
Mutagenesisi | 20 | W → A, M or Y: Nearly abolishes methyltransferase activity with CENPA. 1 Publication | 1 | |
Mutagenesisi | 136 | W → L: Strongly reduces methyltransferase activity with CENPA. 1 Publication | 1 | |
Mutagenesisi | 167 | D → A: Does not affect methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 167 | D → N or Q: Abolishes methyltransferase activity with CENPA. 1 Publication | 1 | |
Mutagenesisi | 168 | N → A: Decreased methyltransferase activity. 2 Publications | 1 | |
Mutagenesisi | 168 | N → K: Loss of methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 177 | D → A: Induces a slight decrease in methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 177 | D → K: Induces a strong decrease in methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 177 | D → N: Strongly reduces methyltransferase activity with CENPA. 1 Publication | 1 | |
Mutagenesisi | 180 | D → A: Induces a decrease in methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 180 | D → K: Induces a strong decrease in methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 180 | D → N: Reduced methyltransferase activity with CENPA. 1 Publication | 1 | |
Mutagenesisi | 182 | S → A: Induces a slight decrease in methyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 182 | S → K: Induces a strong decrease in methyltransferase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 28989 |
OpenTargetsi | ENSG00000148335 |
PharmGKBi | PA162395788 |
Miscellaneous databases
Pharosi | Q9BV86, Tbio |
Polymorphism and mutation databases
BioMutai | NTMT1 |
DMDMi | 74761281 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423228 | 1 – 223 | N-terminal Xaa-Pro-Lys N-methyltransferase 1Add BLAST | 223 | |
Initiator methioninei | Removed; alternateCombined sources1 Publication | |||
ChainiPRO_0000119288 | 2 – 223 | N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedAdd BLAST | 222 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 2 | N-acetylthreonine; in N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedCombined sources1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9BV86 |
jPOSTi | Q9BV86 |
MassIVEi | Q9BV86 |
MaxQBi | Q9BV86 |
PaxDbi | Q9BV86 |
PeptideAtlasi | Q9BV86 |
PRIDEi | Q9BV86 |
ProteomicsDBi | 79180 [Q9BV86-1] 79181 [Q9BV86-2] |
TopDownProteomicsi | Q9BV86-1 [Q9BV86-1] |
PTM databases
iPTMneti | Q9BV86 |
MetOSitei | Q9BV86 |
PhosphoSitePlusi | Q9BV86 |
Expressioni
Gene expression databases
Bgeei | ENSG00000148335, Expressed in left adrenal gland and 213 other tissues |
ExpressionAtlasi | Q9BV86, baseline and differential |
Genevisiblei | Q9BV86, HS |
Organism-specific databases
HPAi | ENSG00000148335, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsQ9BV86
With | #Exp. | IntAct |
---|---|---|
GLYR1 - isoform 4 [Q49A26-4] | 3 | EBI-373016,EBI-12143817 |
RCC1 [P18754] | 4 | EBI-373016,EBI-992720 |
Protein-protein interaction databases
BioGRIDi | 118810, 29 interactors |
DIPi | DIP-31241N |
IntActi | Q9BV86, 14 interactors |
STRINGi | 9606.ENSP00000483489 |
Miscellaneous databases
RNActi | Q9BV86, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9BV86 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9BV86 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 91 – 93 | S-adenosyl-L-methionine bindingCombined sources2 Publications | 3 | |
Regioni | 119 – 120 | S-adenosyl-L-methionine bindingCombined sources2 Publications | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3178, Eukaryota |
GeneTreei | ENSGT00390000008371 |
HOGENOMi | CLU_055356_3_1_1 |
InParanoidi | Q9BV86 |
OMAi | KYWSEVP |
PhylomeDBi | Q9BV86 |
TreeFami | TF314174 |
Family and domain databases
InterProi | View protein in InterPro IPR008576, MeTrfase_NTM1 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR12753, PTHR12753, 1 hit |
Pfami | View protein in Pfam PF05891, Methyltransf_PK, 1 hit |
PIRSFi | PIRSF016958, DUF858_MeTrfase_lik, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9BV86-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTSEVIEDEK QFYSKAKTYW KQIPPTVDGM LGGYGHISSI DINSSRKFLQ
60 70 80 90 100
RFLREGPNKT GTSCALDCGA GIGRITKRLL LPLFREVDMV DITEDFLVQA
110 120 130 140 150
KTYLGEEGKR VRNYFCCGLQ DFTPEPDSYD VIWIQWVIGH LTDQHLAEFL
160 170 180 190 200
RRCKGSLRPN GIIVIKDNMA QEGVILDDVD SSVCRDLDVV RRIICSAGLS
210 220
LLAEERQENL PDEIYHVYSF ALR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketS4R338 | S4R338_HUMAN | N-terminal Xaa-Pro-Lys N-methyltran... | NTMT1 | 135 | Annotation score: | ||
S4R3J7 | S4R3J7_HUMAN | N-terminal Xaa-Pro-Lys N-methyltran... | NTMT1 | 109 | Annotation score: | ||
S4R344 | S4R344_HUMAN | N-terminal Xaa-Pro-Lys N-methyltran... | NTMT1 | 70 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | P → L in BAF85021 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 39 | S → SS in AAF14859 (PubMed:10931946).Curated | 1 | |
Sequence conflicti | 131 | V → A in BAF83646 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039886 | 111 – 223 | VRNYF…SFALR → ATSPISTWPSSCGAARAASA PTASSSSKTTWPRRA in isoform 2. 1 PublicationAdd BLAST | 113 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF110776 mRNA Translation: AAF14859.1 AK290957 mRNA Translation: BAF83646.1 AK292332 mRNA Translation: BAF85021.1 AK298840 mRNA Translation: BAG60968.1 AL590369 Genomic DNA No translation available. BC001396 mRNA Translation: AAH01396.1 BC033234 mRNA Translation: AAH33234.1 |
CCDSi | CCDS35160.1 [Q9BV86-1] CCDS69682.1 [Q9BV86-2] |
RefSeqi | NP_001273725.1, NM_001286796.1 [Q9BV86-1] NP_001273726.1, NM_001286797.1 [Q9BV86-1] NP_001273727.1, NM_001286798.1 [Q9BV86-1] NP_001273728.1, NM_001286799.1 [Q9BV86-1] NP_001273729.1, NM_001286800.1 [Q9BV86-2] NP_001273730.1, NM_001286801.1 [Q9BV86-2] NP_001273731.1, NM_001286802.1 NP_001273732.1, NM_001286803.1 NP_054783.2, NM_014064.3 [Q9BV86-1] |
Genome annotation databases
Ensembli | ENST00000372480; ENSP00000361558; ENSG00000148335 [Q9BV86-1] ENST00000372481; ENSP00000361559; ENSG00000148335 [Q9BV86-2] ENST00000372483; ENSP00000361561; ENSG00000148335 [Q9BV86-1] ENST00000372486; ENSP00000361564; ENSG00000148335 [Q9BV86-1] ENST00000611055; ENSP00000483489; ENSG00000148335 [Q9BV86-1] ENST00000613644; ENSP00000478521; ENSG00000148335 [Q9BV86-1] |
GeneIDi | 28989 |
KEGGi | hsa:28989 |
UCSCi | uc004byd.3, human [Q9BV86-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF110776 mRNA Translation: AAF14859.1 AK290957 mRNA Translation: BAF83646.1 AK292332 mRNA Translation: BAF85021.1 AK298840 mRNA Translation: BAG60968.1 AL590369 Genomic DNA No translation available. BC001396 mRNA Translation: AAH01396.1 BC033234 mRNA Translation: AAH33234.1 |
CCDSi | CCDS35160.1 [Q9BV86-1] CCDS69682.1 [Q9BV86-2] |
RefSeqi | NP_001273725.1, NM_001286796.1 [Q9BV86-1] NP_001273726.1, NM_001286797.1 [Q9BV86-1] NP_001273727.1, NM_001286798.1 [Q9BV86-1] NP_001273728.1, NM_001286799.1 [Q9BV86-1] NP_001273729.1, NM_001286800.1 [Q9BV86-2] NP_001273730.1, NM_001286801.1 [Q9BV86-2] NP_001273731.1, NM_001286802.1 NP_001273732.1, NM_001286803.1 NP_054783.2, NM_014064.3 [Q9BV86-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EX4 | X-ray | 1.75 | A/B | 2-222 | [»] | |
5CVD | X-ray | 1.30 | A/B | 1-223 | [»] | |
5CVE | X-ray | 1.50 | A/B | 1-223 | [»] | |
5E1B | X-ray | 1.65 | A/B | 2-223 | [»] | |
5E1D | X-ray | 1.45 | A/B | 2-223 | [»] | |
5E1M | X-ray | 1.75 | A/B | 2-223 | [»] | |
5E1O | X-ray | 2.00 | A/B | 2-223 | [»] | |
5E2A | X-ray | 1.75 | A/B | 2-223 | [»] | |
5E2B | X-ray | 1.95 | A/B | 2-223 | [»] | |
6DTN | X-ray | 1.48 | B | 2-223 | [»] | |
6KDQ | X-ray | 1.50 | A/B | 1-223 | [»] | |
6PVA | X-ray | 1.84 | B | 2-223 | [»] | |
6PVB | X-ray | 1.50 | B | 2-223 | [»] | |
6WH8 | X-ray | 1.73 | A/B | 2-223 | [»] | |
6WJ7 | X-ray | 1.42 | B | 2-223 | [»] | |
SMRi | Q9BV86 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 118810, 29 interactors |
DIPi | DIP-31241N |
IntActi | Q9BV86, 14 interactors |
STRINGi | 9606.ENSP00000483489 |
PTM databases
iPTMneti | Q9BV86 |
MetOSitei | Q9BV86 |
PhosphoSitePlusi | Q9BV86 |
Polymorphism and mutation databases
BioMutai | NTMT1 |
DMDMi | 74761281 |
Proteomic databases
EPDi | Q9BV86 |
jPOSTi | Q9BV86 |
MassIVEi | Q9BV86 |
MaxQBi | Q9BV86 |
PaxDbi | Q9BV86 |
PeptideAtlasi | Q9BV86 |
PRIDEi | Q9BV86 |
ProteomicsDBi | 79180 [Q9BV86-1] 79181 [Q9BV86-2] |
TopDownProteomicsi | Q9BV86-1 [Q9BV86-1] |
Protocols and materials databases
Antibodypediai | 17874, 152 antibodies |
Genome annotation databases
Ensembli | ENST00000372480; ENSP00000361558; ENSG00000148335 [Q9BV86-1] ENST00000372481; ENSP00000361559; ENSG00000148335 [Q9BV86-2] ENST00000372483; ENSP00000361561; ENSG00000148335 [Q9BV86-1] ENST00000372486; ENSP00000361564; ENSG00000148335 [Q9BV86-1] ENST00000611055; ENSP00000483489; ENSG00000148335 [Q9BV86-1] ENST00000613644; ENSP00000478521; ENSG00000148335 [Q9BV86-1] |
GeneIDi | 28989 |
KEGGi | hsa:28989 |
UCSCi | uc004byd.3, human [Q9BV86-1] |
Organism-specific databases
CTDi | 28989 |
DisGeNETi | 28989 |
EuPathDBi | HostDB:ENSG00000148335.14 |
GeneCardsi | NTMT1 |
HGNCi | HGNC:23373, NTMT1 |
HPAi | ENSG00000148335, Low tissue specificity |
MIMi | 613560, gene |
neXtProti | NX_Q9BV86 |
OpenTargetsi | ENSG00000148335 |
PharmGKBi | PA162395788 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3178, Eukaryota |
GeneTreei | ENSGT00390000008371 |
HOGENOMi | CLU_055356_3_1_1 |
InParanoidi | Q9BV86 |
OMAi | KYWSEVP |
PhylomeDBi | Q9BV86 |
TreeFami | TF314174 |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000148335-MONOMER |
BRENDAi | 2.1.1.244, 2681 |
PathwayCommonsi | Q9BV86 |
Miscellaneous databases
BioGRID-ORCSi | 28989, 4 hits in 846 CRISPR screens |
ChiTaRSi | NTMT1, human |
EvolutionaryTracei | Q9BV86 |
GenomeRNAii | 28989 |
Pharosi | Q9BV86, Tbio |
PROi | PR:Q9BV86 |
RNActi | Q9BV86, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000148335, Expressed in left adrenal gland and 213 other tissues |
ExpressionAtlasi | Q9BV86, baseline and differential |
Genevisiblei | Q9BV86, HS |
Family and domain databases
InterProi | View protein in InterPro IPR008576, MeTrfase_NTM1 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR12753, PTHR12753, 1 hit |
Pfami | View protein in Pfam PF05891, Methyltransf_PK, 1 hit |
PIRSFi | PIRSF016958, DUF858_MeTrfase_lik, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NTM1A_HUMAN | |
Accessioni | Q9BV86Primary (citable) accession number: Q9BV86 Secondary accession number(s): A8K4J2 Q9UI28 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families