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Protein

Vesicle-associated membrane protein 8

Gene

VAMP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).By similarity3 Publications

GO - Molecular functioni

  • chloride channel inhibitor activity Source: UniProtKB
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1236974 ER-Phagosome pathway
R-HSA-421837 Clathrin derived vesicle budding
R-HSA-432720 Lysosome Vesicle Biogenesis
R-HSA-432722 Golgi Associated Vesicle Biogenesis
R-HSA-6798695 Neutrophil degranulation
R-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis

Protein family/group databases

TCDBi1.F.1.1.1 the synaptosomal vesicle fusion pore (svf-pore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 81 Publication
Short name:
VAMP-81 Publication
Alternative name(s):
Endobrevin1 Publication
Short name:
EDB1 Publication
Gene namesi
Name:VAMP81 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000118640.10
HGNCiHGNC:12647 VAMP8
MIMi603177 gene
neXtProtiNX_Q9BV40

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75CytoplasmicSequence analysisAdd BLAST75
Transmembranei76 – 96Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini97 – 100VesicularSequence analysis4

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8673
OpenTargetsiENSG00000118640
PharmGKBiPA37271

Polymorphism and mutation databases

BioMutaiVAMP8
DMDMi55976764

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067361 – 100Vesicle-associated membrane protein 8Add BLAST100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei18PhosphoserineCombined sources1
Modified residuei28PhosphothreonineCombined sources1
Modified residuei48PhosphothreonineCombined sources1
Modified residuei54PhosphothreonineCombined sources1
Modified residuei55PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BV40
MaxQBiQ9BV40
PaxDbiQ9BV40
PeptideAtlasiQ9BV40
PRIDEiQ9BV40
ProteomicsDBi79166
TopDownProteomicsiQ9BV40

PTM databases

iPTMnetiQ9BV40
PhosphoSitePlusiQ9BV40

Expressioni

Tissue specificityi

Platelets.1 Publication

Gene expression databases

BgeeiENSG00000118640 Expressed in 220 organ(s), highest expression level in palpebral conjunctiva
CleanExiHS_VAMP8
ExpressionAtlasiQ9BV40 baseline and differential
GenevisibleiQ9BV40 HS

Organism-specific databases

HPAiCAB037132
CAB037147
HPA006882

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (PubMed:25686604). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:12130530).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with STX8By similarity1

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114221, 40 interactors
CORUMiQ9BV40
DIPiDIP-40358N
IntActiQ9BV40, 21 interactors
MINTiQ9BV40
STRINGi9606.ENSP00000263864

Structurei

Secondary structure

1100
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BV40
SMRiQ9BV40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 72v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiQ9BV40
KOiK08512
OMAiKMIVIIC
OrthoDBiEOG091G0MPE
PhylomeDBiQ9BV40
TreeFamiTF320419

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

Q9BV40-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED
60 70 80 90 100
LEATSEHFKT TSQKVARKFW WKNVKMIVLI CVIVFIIILF IVLFATGAFS
Length:100
Mass (Da):11,438
Last modified:June 1, 2001 - v1
Checksum:i018B986442BBD9B6
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JXZ5C9JXZ5_HUMAN
Vesicle-associated membrane protein...
VAMP8
74Annotation score:
B8ZZT4B8ZZT4_HUMAN
Vesicle-associated membrane protein...
VAMP8
99Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12R → L in AAC08434 (PubMed:9614193).Curated1
Sequence conflicti81C → R in CAG33276 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053233 mRNA Translation: AAC08434.1
BT006700 mRNA Translation: AAP35346.1
CR456995 mRNA Translation: CAG33276.1
AC016753 Genomic DNA Translation: AAY24341.1
BC001634 mRNA Translation: AAH01634.1
CCDSiCCDS1979.1
RefSeqiNP_003752.2, NM_003761.4
UniGeneiHs.714302

Genome annotation databases

EnsembliENST00000263864; ENSP00000263864; ENSG00000118640
GeneIDi8673
KEGGihsa:8673
UCSCiuc002spt.5 human

Similar proteinsi

Entry informationi

Entry nameiVAMP8_HUMAN
AccessioniPrimary (citable) accession number: Q9BV40
Secondary accession number(s): O60625, Q53SP9, Q6IB09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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