UniProtKB - Q9BUT1 (BDH2_HUMAN)
Protein
3-hydroxybutyrate dehydrogenase type 2
Gene
BDH2
Organism
Homo sapiens (Human)
Status
Functioni
Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis. Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (By similarity). Also acts as a 3-hydroxybutyrate dehydrogenase (PubMed:16380372).By similarity1 Publication
Catalytic activityi
- EC:1.1.1.301 Publication
Kineticsi
- KM=60 µM for NAD1 Publication
- KM=10 mM for R-hydroxybutyrate1 Publication
- Vmax=82 nmol/min/mg enzyme (at 30 degrees Celsius)1 Publication
pH dependencei
Optimum pH is 7.5-9.0.1 Publication
: Siderophore biosynthesis Pathwayi
This protein is involved in Siderophore biosynthesis.By similarityView all proteins of this organism that are known to be involved in Siderophore biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | NADCombined sources1 Publication | 1 | |
Binding sitei | 58 | NADCombined sources1 Publication | 1 | |
Binding sitei | 144 | SubstrateCurated | 1 | |
Active sitei | 147 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 151 | NADCombined sources1 Publication | 1 | |
Binding sitei | 188 | SubstrateCurated | 1 | |
Binding sitei | 205 | SubstrateCurated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 16 – 18 | NADCombined sources1 Publication | 3 | |
Nucleotide bindingi | 180 – 184 | NADCombined sources1 Publication | 5 |
GO - Molecular functioni
- 3-hydroxybutyrate dehydrogenase activity Source: HGNC-UCL
- NAD binding Source: HGNC-UCL
- oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: GO_Central
GO - Biological processi
- epithelial cell differentiation Source: UniProtKB
- fatty acid beta-oxidation Source: HGNC-UCL
- heme metabolic process Source: UniProtKB
- iron ion homeostasis Source: UniProtKB
- ketone body biosynthetic process Source: Reactome
- siderophore biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | MetaCyc:HS08987-MONOMER |
PathwayCommonsi | Q9BUT1 |
Reactomei | R-HSA-77111, Synthesis of Ketone Bodies |
SABIO-RKi | Q9BUT1 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:BDH2 Synonyms:DHRS61 Publication, SDR15C1 ORF Names:UNQ6308/PRO20933 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000164039.14 |
HGNCi | HGNC:32389, BDH2 |
neXtProti | NX_Q9BUT1 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasm Source: HGNC-UCL
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 56898 |
OpenTargetsi | ENSG00000164039 |
PharmGKBi | PA142672559 |
Miscellaneous databases
Pharosi | Q9BUT1, Tbio |
Polymorphism and mutation databases
BioMutai | BDH2 |
DMDMi | 125987797 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000042580 | 1 – 245 | 3-hydroxybutyrate dehydrogenase type 2Add BLAST | 245 |
Proteomic databases
EPDi | Q9BUT1 |
jPOSTi | Q9BUT1 |
MassIVEi | Q9BUT1 |
MaxQBi | Q9BUT1 |
PaxDbi | Q9BUT1 |
PeptideAtlasi | Q9BUT1 |
PRIDEi | Q9BUT1 |
ProteomicsDBi | 79125 [Q9BUT1-1] 79126 [Q9BUT1-2] 79127 [Q9BUT1-3] |
PTM databases
iPTMneti | Q9BUT1 |
PhosphoSitePlusi | Q9BUT1 |
Expressioni
Tissue specificityi
Detected in liver (at protein level).1 Publication
Inductioni
Induced by low iron levels, and down-regulated by elevated iron levels.1 Publication
Gene expression databases
Bgeei | ENSG00000164039, Expressed in adult mammalian kidney and 117 other tissues |
ExpressionAtlasi | Q9BUT1, baseline and differential |
Genevisiblei | Q9BUT1, HS |
Organism-specific databases
HPAi | ENSG00000164039, Tissue enhanced (kidney) |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationBinary interactionsi
Q9BUT1
With | #Exp. | IntAct |
---|---|---|
NFYC - isoform 1 [Q13952-2] | 3 | EBI-6137689,EBI-11956831 |
Protein-protein interaction databases
BioGRIDi | 121228, 13 interactors |
IntActi | Q9BUT1, 9 interactors |
STRINGi | 9606.ENSP00000296424 |
Miscellaneous databases
RNActi | Q9BUT1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9BUT1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9BUT1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
GeneTreei | ENSGT00940000156721 |
HOGENOMi | CLU_010194_1_0_1 |
InParanoidi | Q9BUT1 |
OMAi | FPQWGAY |
OrthoDBi | 1226147at2759 |
PhylomeDBi | Q9BUT1 |
TreeFami | TF328795 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9BUT1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP
60 70 80 90 100
GIQTRVLDVT KKKQIDQFAN EVERLDVLFN VAGFVHHGTV LDCEEKDWDF
110 120 130 140 150
SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT
160 170 180 190 200
KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN
210 220 230 240
DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6R9U8 | D6R9U8_HUMAN | 3-hydroxybutyrate dehydrogenase typ... | BDH2 | 116 | Annotation score: | ||
D6RIR6 | D6RIR6_HUMAN | 3-hydroxybutyrate dehydrogenase typ... | BDH2 | 130 | Annotation score: | ||
D6RBF6 | D6RBF6_HUMAN | 3-hydroxybutyrate dehydrogenase typ... | BDH2 | 64 | Annotation score: | ||
D6RFG2 | D6RFG2_HUMAN | 3-hydroxybutyrate dehydrogenase typ... | BDH2 | 90 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 95 | E → V in BAF82849 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 161 | V → L in AAQ89200 (PubMed:12975309).Curated | 1 | |
Sequence conflicti | 203 | L → P in BAF82849 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 230 | A → T in AAH95414 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_023602 | 70 | N → S4 PublicationsCorresponds to variant dbSNP:rs1054707Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039781 | 1 – 101 | Missing in isoform 3. 1 PublicationAdd BLAST | 101 | |
Alternative sequenceiVSP_015859 | 141 – 245 | VVNRC…GGWSL → GSVSFRGLRCSYTHIIKSSA FGNRHSRDYNFKY in isoform 2. 3 PublicationsAdd BLAST | 105 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF164790 mRNA Translation: AAF80754.1 AY358841 mRNA Translation: AAQ89200.1 CR457309 mRNA Translation: CAG33590.1 AK023323 mRNA Translation: BAB14526.1 AK290160 mRNA Translation: BAF82849.1 AK300626 mRNA Translation: BAG62318.1 AC097485 Genomic DNA No translation available. BC037277 mRNA Translation: AAH37277.1 BC001953 mRNA Translation: AAH01953.1 BC095414 mRNA Translation: AAH95414.1 |
CCDSi | CCDS3663.1 [Q9BUT1-1] |
RefSeqi | NP_064524.3, NM_020139.3 [Q9BUT1-1] XP_011530430.1, XM_011532128.2 [Q9BUT1-1] XP_016863951.1, XM_017008462.1 [Q9BUT1-1] |
Genome annotation databases
Ensembli | ENST00000296424; ENSP00000296424; ENSG00000164039 [Q9BUT1-1] |
GeneIDi | 56898 |
KEGGi | hsa:56898 |
UCSCi | uc003hwz.4, human [Q9BUT1-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF164790 mRNA Translation: AAF80754.1 AY358841 mRNA Translation: AAQ89200.1 CR457309 mRNA Translation: CAG33590.1 AK023323 mRNA Translation: BAB14526.1 AK290160 mRNA Translation: BAF82849.1 AK300626 mRNA Translation: BAG62318.1 AC097485 Genomic DNA No translation available. BC037277 mRNA Translation: AAH37277.1 BC001953 mRNA Translation: AAH01953.1 BC095414 mRNA Translation: AAH95414.1 |
CCDSi | CCDS3663.1 [Q9BUT1-1] |
RefSeqi | NP_064524.3, NM_020139.3 [Q9BUT1-1] XP_011530430.1, XM_011532128.2 [Q9BUT1-1] XP_016863951.1, XM_017008462.1 [Q9BUT1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AG5 | X-ray | 1.84 | A/B/C/D | 1-245 | [»] | |
SMRi | Q9BUT1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121228, 13 interactors |
IntActi | Q9BUT1, 9 interactors |
STRINGi | 9606.ENSP00000296424 |
PTM databases
iPTMneti | Q9BUT1 |
PhosphoSitePlusi | Q9BUT1 |
Polymorphism and mutation databases
BioMutai | BDH2 |
DMDMi | 125987797 |
Proteomic databases
EPDi | Q9BUT1 |
jPOSTi | Q9BUT1 |
MassIVEi | Q9BUT1 |
MaxQBi | Q9BUT1 |
PaxDbi | Q9BUT1 |
PeptideAtlasi | Q9BUT1 |
PRIDEi | Q9BUT1 |
ProteomicsDBi | 79125 [Q9BUT1-1] 79126 [Q9BUT1-2] 79127 [Q9BUT1-3] |
Protocols and materials databases
Antibodypediai | 26090, 192 antibodies |
Genome annotation databases
Ensembli | ENST00000296424; ENSP00000296424; ENSG00000164039 [Q9BUT1-1] |
GeneIDi | 56898 |
KEGGi | hsa:56898 |
UCSCi | uc003hwz.4, human [Q9BUT1-1] |
Organism-specific databases
CTDi | 56898 |
DisGeNETi | 56898 |
EuPathDBi | HostDB:ENSG00000164039.14 |
GeneCardsi | BDH2 |
HGNCi | HGNC:32389, BDH2 |
HPAi | ENSG00000164039, Tissue enhanced (kidney) |
neXtProti | NX_Q9BUT1 |
OpenTargetsi | ENSG00000164039 |
PharmGKBi | PA142672559 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
GeneTreei | ENSGT00940000156721 |
HOGENOMi | CLU_010194_1_0_1 |
InParanoidi | Q9BUT1 |
OMAi | FPQWGAY |
OrthoDBi | 1226147at2759 |
PhylomeDBi | Q9BUT1 |
TreeFami | TF328795 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS08987-MONOMER |
PathwayCommonsi | Q9BUT1 |
Reactomei | R-HSA-77111, Synthesis of Ketone Bodies |
SABIO-RKi | Q9BUT1 |
Miscellaneous databases
BioGRID-ORCSi | 56898, 8 hits in 842 CRISPR screens |
ChiTaRSi | BDH2, human |
EvolutionaryTracei | Q9BUT1 |
GenomeRNAii | 56898 |
Pharosi | Q9BUT1, Tbio |
PROi | PR:Q9BUT1 |
RNActi | Q9BUT1, protein |
Gene expression databases
Bgeei | ENSG00000164039, Expressed in adult mammalian kidney and 117 other tissues |
ExpressionAtlasi | Q9BUT1, baseline and differential |
Genevisiblei | Q9BUT1, HS |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BDH2_HUMAN | |
Accessioni | Q9BUT1Primary (citable) accession number: Q9BUT1 Secondary accession number(s): A8K295 Q9NRX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2005 |
Last sequence update: | February 6, 2007 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations