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Protein

DNA-directed RNA polymerase III subunit RPC3

Gene

POLR3C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628).3 Publications

Miscellaneous

Antibodies against POLR3C have been found in the sera of patients with systemic sclerosis (SSc).

GO - Molecular functioni

  • DNA-directed 5'-3' RNA polymerase activity Source: ProtInc
  • single-stranded DNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processAntiviral defense, Immunity, Innate immunity, Transcription

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC3
Short name:
RNA polymerase III subunit C3
Alternative name(s):
DNA-directed RNA polymerase III subunit C
RNA polymerase III 62 kDa subunit
Short name:
RPC62
Gene namesi
Name:POLR3C
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000186141.8
HGNCiHGNC:30076 POLR3C
MIMi617454 gene
neXtProtiNX_Q9BUI4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51 – 52KK → EE: Strongly decreased ssDNA-binding. No effect on interaction with POLR3F, POLR3G, nor with POLR3GL. 1 Publication2
Mutagenesisi312L → K: Loss of interaction with POLR3G and POLR3GL. No effect on interaction with POLR3F. 1 Publication1
Mutagenesisi357R → E: Strongly decreased ssDNA-binding. No effect on interaction with POLR3F, POLR3G, nor with POLR3GL. 1 Publication1
Mutagenesisi364 – 367RIFR → EIFE: Strongly decreased ssDNA-binding. No effect on interaction with POLR3F, POLR3G, nor with POLR3GL. 1 Publication4
Mutagenesisi389K → E: Strongly decreased ssDNA-binding. No effect on interaction with POLR3F, POLR3G, nor with POLR3GL. 1 Publication1
Mutagenesisi445 – 449NLIER → ALIEE: Strongly decreased ssDNA-binding. No effect on interaction with POLR3F, POLR3G, nor with POLR3GL. 1 Publication5
Mutagenesisi466 – 470RVEAI → EVEAF: Mild decrease in ssDNA-binding. No effect on interaction with POLR3F, POLR3G, nor with POLR3GL. 1 Publication5

Organism-specific databases

OpenTargetsiENSG00000186141
PharmGKBiPA134870963

Polymorphism and mutation databases

BioMutaiPOLR3C
DMDMi60393871

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739631 – 534DNA-directed RNA polymerase III subunit RPC3Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei194PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BUI4
MaxQBiQ9BUI4
PaxDbiQ9BUI4
PeptideAtlasiQ9BUI4
PRIDEiQ9BUI4
ProteomicsDBi79088

PTM databases

GlyConnecti1184
iPTMnetiQ9BUI4
PhosphoSitePlusiQ9BUI4

Expressioni

Gene expression databases

BgeeiENSG00000186141 Expressed in 208 organ(s), highest expression level in right testis
CleanExiHS_POLR3C
ExpressionAtlasiQ9BUI4 baseline and differential
GenevisibleiQ9BUI4 HS

Organism-specific databases

HPAiHPA027508
HPA027516

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex (PubMed:9171375, PubMed:12391170). Directly interacts with POLR3G and POLR3GL (PubMed:24107381, PubMed:21358628, PubMed:26394183). Directly interacts with POLR3F/RPC39 (PubMed:26394183). Interacts with GTF3C4 (PubMed:10523658).By similarity6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi115868, 43 interactors
DIPiDIP-59078N
IntActiQ9BUI4, 38 interactors
STRINGi9606.ENSP00000334564

Structurei

Secondary structure

1534
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BUI4
SMRiQ9BUI4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BUI4

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2587 Eukaryota
ENOG410XPVH LUCA
GeneTreeiENSGT00390000002799
HOGENOMiHOG000046475
HOVERGENiHBG059543
InParanoidiQ9BUI4
KOiK03023
OMAiQHNLVIY
OrthoDBiEOG091G186Z
PhylomeDBiQ9BUI4
TreeFamiTF103048

Family and domain databases

Gene3Di1.10.10.10, 4 hits
InterProiView protein in InterPro
IPR013197 RNA_pol_III_RPC82-rel_HTH
IPR008806 RNA_pol_III_Rpc82_C
IPR039748 RPC3
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR12949 PTHR12949, 1 hit
PfamiView protein in Pfam
PF08221 HTH_9, 1 hit
PF05645 RNA_pol_Rpc82, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9BUI4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTQAEIKLCS LLLQEHFGEI VEKIGVHLIR TGSQPLRVIA HDTGTSLDQV
60 70 80 90 100
KKALCVLVQH NLVSYQVHKR GVVEYEAQCS RVLRMLRYPR YIYTTKTLYS
110 120 130 140 150
DTGELIVEEL LLNGKLTMSA VVKKVADRLT ETMEDGKTMD YAEVSNTFVR
160 170 180 190 200
LADTHFVQRC PSVPTTENSD PGPPPPAPTL VINEKDMYLV PKLSLIGKGK
210 220 230 240 250
RRRSSDEDAA GEPKAKRPKY TTDNKEPIPD DGIYWQANLD RFHQHFRDQA
260 270 280 290 300
IVSAVANRMD QTSSEIVRTM LRMSEITTSS SAPFTQPLSS NEIFRSLPVG
310 320 330 340 350
YNISKQVLDQ YLTLLADDPL EFVGKSGDSG GGMYVINLHK ALASLATATL
360 370 380 390 400
ESVVQERFGS RCARIFRLVL QKKHIEQKQV EDFAMIPAKE AKDMLYKMLS
410 420 430 440 450
ENFMSLQEIP KTPDHAPSRT FYLYTVNILS AARMLLHRCY KSIANLIERR
460 470 480 490 500
QFETKENKRL LEKSQRVEAI IASMQATGAE EAQLQEIEEM ITAPERQQLE
510 520 530
TLKRNVNKLD ASEIQVDETI FLLESYIECT MKRQ
Length:534
Mass (Da):60,612
Last modified:June 1, 2001 - v1
Checksum:i0E4CFEE295EE1A55
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PHH9E9PHH9_HUMAN
DNA-directed RNA polymerase III sub...
POLR3C
411Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85 – 86ML → IV in AAB63675 (PubMed:9171375).Curated2
Sequence conflicti119 – 120SA → CT in AAB63675 (PubMed:9171375).Curated2
Sequence conflicti216 – 237KRPKY…IYWQA → RDQNILQITRXPFQMMGFIG RP in AAB63675 (PubMed:9171375).CuratedAdd BLAST22
Sequence conflicti289S → F in AAB63675 (PubMed:9171375).Curated1
Sequence conflicti343A → R in AAB63675 (PubMed:9171375).Curated1
Sequence conflicti366F → C in AAB63675 (PubMed:9171375).Curated1
Sequence conflicti387 – 389PAK → LQ in AAB63675 (PubMed:9171375).Curated3
Sequence conflicti401E → G in AAB63675 (PubMed:9171375).Curated1
Sequence conflicti436 – 437LH → FD in AAB63675 (PubMed:9171375).Curated2
Sequence conflicti517D → E in CAB41919 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019083243H → R1 PublicationCorresponds to variant dbSNP:rs1044697Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93867 mRNA Translation: AAB63675.1
AY091463 mRNA Translation: AAM12033.1
AJ238221
, AJ238222, AJ238223, AJ238224, AJ238225, AJ238226, AJ238227, AJ238228, AJ238229, AJ238230, AJ238231, AJ238232, AJ238233, AJ238234 Genomic DNA Translation: CAB41919.1
BC002586 mRNA Translation: AAH02586.1
CCDSiCCDS72864.1
RefSeqiNP_001290385.1, NM_001303456.1
NP_006459.3, NM_006468.7
UniGeneiHs.515768
Hs.591457

Genome annotation databases

EnsembliENST00000334163; ENSP00000334564; ENSG00000186141
GeneIDi10623
KEGGihsa:10623
UCSCiuc001eoh.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93867 mRNA Translation: AAB63675.1
AY091463 mRNA Translation: AAM12033.1
AJ238221
, AJ238222, AJ238223, AJ238224, AJ238225, AJ238226, AJ238227, AJ238228, AJ238229, AJ238230, AJ238231, AJ238232, AJ238233, AJ238234 Genomic DNA Translation: CAB41919.1
BC002586 mRNA Translation: AAH02586.1
CCDSiCCDS72864.1
RefSeqiNP_001290385.1, NM_001303456.1
NP_006459.3, NM_006468.7
UniGeneiHs.515768
Hs.591457

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XUBX-ray2.80A1-534[»]
2XV4X-ray2.95S1-534[»]
5AFQX-ray7.00A/B1-534[»]
ProteinModelPortaliQ9BUI4
SMRiQ9BUI4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115868, 43 interactors
DIPiDIP-59078N
IntActiQ9BUI4, 38 interactors
STRINGi9606.ENSP00000334564

PTM databases

GlyConnecti1184
iPTMnetiQ9BUI4
PhosphoSitePlusiQ9BUI4

Polymorphism and mutation databases

BioMutaiPOLR3C
DMDMi60393871

Proteomic databases

EPDiQ9BUI4
MaxQBiQ9BUI4
PaxDbiQ9BUI4
PeptideAtlasiQ9BUI4
PRIDEiQ9BUI4
ProteomicsDBi79088

Protocols and materials databases

DNASUi10623
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334163; ENSP00000334564; ENSG00000186141
GeneIDi10623
KEGGihsa:10623
UCSCiuc001eoh.3 human

Organism-specific databases

CTDi10623
EuPathDBiHostDB:ENSG00000186141.8
GeneCardsiPOLR3C
HGNCiHGNC:30076 POLR3C
HPAiHPA027508
HPA027516
MIMi617454 gene
neXtProtiNX_Q9BUI4
OpenTargetsiENSG00000186141
PharmGKBiPA134870963
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2587 Eukaryota
ENOG410XPVH LUCA
GeneTreeiENSGT00390000002799
HOGENOMiHOG000046475
HOVERGENiHBG059543
InParanoidiQ9BUI4
KOiK03023
OMAiQHNLVIY
OrthoDBiEOG091G186Z
PhylomeDBiQ9BUI4
TreeFamiTF103048

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Miscellaneous databases

ChiTaRSiPOLR3C human
EvolutionaryTraceiQ9BUI4
GeneWikiiPOLR3C
GenomeRNAii10623
PROiPR:Q9BUI4
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186141 Expressed in 208 organ(s), highest expression level in right testis
CleanExiHS_POLR3C
ExpressionAtlasiQ9BUI4 baseline and differential
GenevisibleiQ9BUI4 HS

Family and domain databases

Gene3Di1.10.10.10, 4 hits
InterProiView protein in InterPro
IPR013197 RNA_pol_III_RPC82-rel_HTH
IPR008806 RNA_pol_III_Rpc82_C
IPR039748 RPC3
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR12949 PTHR12949, 1 hit
PfamiView protein in Pfam
PF08221 HTH_9, 1 hit
PF05645 RNA_pol_Rpc82, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPC3_HUMAN
AccessioniPrimary (citable) accession number: Q9BUI4
Secondary accession number(s): O15317, Q9Y3R6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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