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Protein

Protein crumbs homolog 3

Gene

CRB3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions.2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: BHF-UCL
  • SH3 domain binding Source: BHF-UCL

GO - Biological processi

  • bicellular tight junction assembly Source: Reactome
  • protein localization to plasma membrane Source: BHF-UCL

Enzyme and pathway databases

ReactomeiR-HSA-420029 Tight junction interactions
SIGNORiQ9BUF7

Names & Taxonomyi

Protein namesi
Recommended name:
Protein crumbs homolog 3
Gene namesi
Name:CRB3
ORF Names:UNQ588/PRO1158
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000130545.15
HGNCiHGNC:20237 CRB3
MIMi609737 gene
neXtProtiNX_Q9BUF7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 59ExtracellularSequence analysisAdd BLAST33
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 120CytoplasmicSequence analysisAdd BLAST40

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36N → D: Abolishes N-glycosylation. 1 Publication1
Mutagenesisi117 – 120Missing : Loss of interaction with PARD6A and MPP5. 2 Publications4

Organism-specific databases

DisGeNETi92359
OpenTargetsiENSG00000130545
PharmGKBiPA134862130

Polymorphism and mutation databases

BioMutaiCRB3
DMDMi67460584

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000002100527 – 120Protein crumbs homolog 3Add BLAST94

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9BUF7
MaxQBiQ9BUF7
PeptideAtlasiQ9BUF7
PRIDEiQ9BUF7
ProteomicsDBi79082
79083 [Q9BUF7-2]

PTM databases

iPTMnetiQ9BUF7
PhosphoSitePlusiQ9BUF7

Expressioni

Tissue specificityi

Preferentially expressed in epithelial tissues. Expressed at high levels in lung, kidney, retina, colon and mammary glands. Expressed at moderate levels in liver, spleen, pancreas, placenta and prostate.2 Publications

Gene expression databases

BgeeiENSG00000130545 Expressed in 178 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_CRB3
GenevisibleiQ9BUF7 HS

Organism-specific databases

HPAiHPA013835

Interactioni

Subunit structurei

Interacts with MPP5, PARD6A and PATJ.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PSMA3P257883EBI-9844372,EBI-348380

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124940, 3 interactors
IntActiQ9BUF7, 22 interactors
MINTiQ9BUF7

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BUF7
SMRiQ9BUF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi117 – 120PDZ-binding4

Domaini

The PDZ-binding motif is involved in the interactions with PARD6A and MPP5.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000012998
HOGENOMiHOG000013209
HOVERGENiHBG081347
InParanoidiQ9BUF7
KOiK06090
OMAiWGQVWGQ
OrthoDBiEOG091G14HT
PhylomeDBiQ9BUF7
TreeFamiTF353183

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9BUF7-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MANPGLGLLL ALGLPFLLAR WGRAWGQIQT TSANENSTVL PSSTSSSSDG
60 70 80 90 100
NLRPEAITAI IVVFSLLAAL LLAVGLALLV RKLREKRQTE GTYRPSSEEQ
110 120
VGARVPPTPN LKLPPEERLI
Length:120
Mass (Da):12,854
Last modified:June 7, 2005 - v3
Checksum:iF0CCDE3D91E7392D
GO
Isoform 2 (identifier: Q9BUF7-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     101-120: VGARVPPTPNLKLPPEERLI → FSHAAEARAPQDSKETVQGCLPI

Show »
Length:123
Mass (Da):13,113
Checksum:iD43DADF94F36303E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013989101 – 120VGARV…EERLI → FSHAAEARAPQDSKETVQGC LPI in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY103469 mRNA Translation: AAM44074.1
AF503290 mRNA Translation: AAM23013.1
AY358684 mRNA Translation: AAQ89047.1
AK292956 mRNA Translation: BAF85645.1
CH471139 Genomic DNA Translation: EAW69084.1
CH471139 Genomic DNA Translation: EAW69085.1
CH471139 Genomic DNA Translation: EAW69086.1
BC002652 mRNA Translation: AAH02652.2
BC018409 mRNA Translation: AAH18409.1
CCDSiCCDS12166.1 [Q9BUF7-2]
CCDS12167.1 [Q9BUF7-1]
RefSeqiNP_631900.1, NM_139161.4 [Q9BUF7-1]
NP_777377.1, NM_174881.3 [Q9BUF7-2]
NP_777378.1, NM_174882.2 [Q9BUF7-1]
UniGeneiHs.150319

Genome annotation databases

EnsembliENST00000308243; ENSP00000310123; ENSG00000130545 [Q9BUF7-1]
ENST00000356762; ENSP00000349204; ENSG00000130545 [Q9BUF7-2]
ENST00000598494; ENSP00000469707; ENSG00000130545 [Q9BUF7-1]
ENST00000600229; ENSP00000472010; ENSG00000130545 [Q9BUF7-1]
GeneIDi92359
KEGGihsa:92359
UCSCiuc002mey.4 human [Q9BUF7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY103469 mRNA Translation: AAM44074.1
AF503290 mRNA Translation: AAM23013.1
AY358684 mRNA Translation: AAQ89047.1
AK292956 mRNA Translation: BAF85645.1
CH471139 Genomic DNA Translation: EAW69084.1
CH471139 Genomic DNA Translation: EAW69085.1
CH471139 Genomic DNA Translation: EAW69086.1
BC002652 mRNA Translation: AAH02652.2
BC018409 mRNA Translation: AAH18409.1
CCDSiCCDS12166.1 [Q9BUF7-2]
CCDS12167.1 [Q9BUF7-1]
RefSeqiNP_631900.1, NM_139161.4 [Q9BUF7-1]
NP_777377.1, NM_174881.3 [Q9BUF7-2]
NP_777378.1, NM_174882.2 [Q9BUF7-1]
UniGeneiHs.150319

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I7ZX-ray1.80B113-120[»]
ProteinModelPortaliQ9BUF7
SMRiQ9BUF7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124940, 3 interactors
IntActiQ9BUF7, 22 interactors
MINTiQ9BUF7

PTM databases

iPTMnetiQ9BUF7
PhosphoSitePlusiQ9BUF7

Polymorphism and mutation databases

BioMutaiCRB3
DMDMi67460584

Proteomic databases

EPDiQ9BUF7
MaxQBiQ9BUF7
PeptideAtlasiQ9BUF7
PRIDEiQ9BUF7
ProteomicsDBi79082
79083 [Q9BUF7-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308243; ENSP00000310123; ENSG00000130545 [Q9BUF7-1]
ENST00000356762; ENSP00000349204; ENSG00000130545 [Q9BUF7-2]
ENST00000598494; ENSP00000469707; ENSG00000130545 [Q9BUF7-1]
ENST00000600229; ENSP00000472010; ENSG00000130545 [Q9BUF7-1]
GeneIDi92359
KEGGihsa:92359
UCSCiuc002mey.4 human [Q9BUF7-1]

Organism-specific databases

CTDi92359
DisGeNETi92359
EuPathDBiHostDB:ENSG00000130545.15
GeneCardsiCRB3
HGNCiHGNC:20237 CRB3
HPAiHPA013835
MIMi609737 gene
neXtProtiNX_Q9BUF7
OpenTargetsiENSG00000130545
PharmGKBiPA134862130
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000012998
HOGENOMiHOG000013209
HOVERGENiHBG081347
InParanoidiQ9BUF7
KOiK06090
OMAiWGQVWGQ
OrthoDBiEOG091G14HT
PhylomeDBiQ9BUF7
TreeFamiTF353183

Enzyme and pathway databases

ReactomeiR-HSA-420029 Tight junction interactions
SIGNORiQ9BUF7

Miscellaneous databases

GeneWikiiCRB3
GenomeRNAii92359
PROiPR:Q9BUF7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130545 Expressed in 178 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_CRB3
GenevisibleiQ9BUF7 HS

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiCRUM3_HUMAN
AccessioniPrimary (citable) accession number: Q9BUF7
Secondary accession number(s): A8KA91
, D6W643, Q8N0V8, Q8WVA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 12, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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