CRB3

Functionⁱ

Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions.2 Publications

GO - Molecular functionⁱ

protein domain specific binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 17332497
SH3 domain binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 17332497

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

bicellular tight junction assembly Source: Reactome
protein localization to plasma membrane
Source: BHF-UCL
Inferred from direct assayⁱ
- 17332497

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

Reactomeⁱ	R-HSA-420029 Tight junction interactions
SIGNORⁱ	Q9BUF7

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein crumbs homolog 3
Gene namesⁱ	Name:CRB3 ORF Names:UNQ588/PRO1158
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000130545.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:20237 CRB3
MIMⁱ	609737 gene
neXtProtⁱ	NX_Q9BUF7

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	27 – 59	ExtracellularSequence analysisAdd BLAST	33
Transmembraneⁱ	60 – 80	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	81 – 120	CytoplasmicSequence analysisAdd BLAST	40

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	36	N → D: Abolishes N-glycosylation. 1 Publication		1
Mutagenesisⁱ	117 – 120	Missing : Loss of interaction with PARD6A and MPP5. 2 Publications		4

Organism-specific databases

DisGeNET More...DisGeNETⁱ	92359
Open Targets More...OpenTargetsⁱ	ENSG00000130545
PharmGKBⁱ	PA134862130

Polymorphism and mutation databases

BioMutaⁱ	CRB3
DMDMⁱ	67460584

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 26	Sequence analysisAdd BLAST		26
ChainⁱPRO_0000021005	27 – 120	Protein crumbs homolog 3Add BLAST		94

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	36	N-linked (GlcNAc...) asparagine1 Publication		1

Keywords - PTMⁱ

Glycoprotein

Proteomic databases

EPDⁱ	Q9BUF7
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9BUF7
PeptideAtlas More...PeptideAtlasⁱ	Q9BUF7
PRIDEⁱ	Q9BUF7
ProteomicsDBⁱ	79082 79083 [Q9BUF7-2]

PTM databases

iPTMnetⁱ	Q9BUF7
PhosphoSitePlusⁱ	Q9BUF7

Expressionⁱ

Tissue specificityⁱ

Preferentially expressed in epithelial tissues. Expressed at high levels in lung, kidney, retina, colon and mammary glands. Expressed at moderate levels in liver, spleen, pancreas, placenta and prostate.2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000130545 Expressed in 178 organ(s), highest expression level in lower esophagus mucosa
CleanExⁱ	HS_CRB3
Genevisibleⁱ	Q9BUF7 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA013835

HPAⁱ

HPA013835

Interactionⁱ

Subunit structureⁱ

Interacts with MPP5, PARD6A and PATJ.3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
PSMA3	P25788	3	EBI-9844372,EBI-348380

With

Entry

#Exp.

IntAct

Notes

PSMA3

P25788

3

EBI-9844372,EBI-348380

GO - Molecular functionⁱ

protein domain specific binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 17332497
SH3 domain binding
Source: BHF-UCL
Inferred from physical interactionⁱ
- 17332497

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	124940, 3 interactors
IntActⁱ	Q9BUF7, 22 interactors
Molecular INTeraction database More...MINTⁱ	Q9BUF7

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Beta strandⁱ	116 – 120	Combined sources		5

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9BUF7
SMRⁱ	Q9BUF7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	117 – 120	PDZ-binding		4

Domainⁱ

The PDZ-binding motif is involved in the interactions with PARD6A and MPP5.

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000012998
HOGENOMⁱ	HOG000013209
HOVERGENⁱ	HBG081347
InParanoidⁱ	Q9BUF7
KEGG Orthology (KO) More...KOⁱ	K06090
OMAⁱ	WGQVWGQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G14HT
PhylomeDBⁱ	Q9BUF7
TreeFamⁱ	TF353183

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

Isoform 1 (identifier: Q9BUF7-1) [UniParc]FASTA Add to basket

Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANPGLGLLL ALGLPFLLAR WGRAWGQIQT TSANENSTVL PSSTSSSSDG 
        60         70         80         90        100
NLRPEAITAI IVVFSLLAAL LLAVGLALLV RKLREKRQTE GTYRPSSEEQ 
       110        120
VGARVPPTPN LKLPPEERLI

Length:120

Mass (Da):12,854

Last modified:June 7, 2005 - v3

Checksum:ⁱF0CCDE3D91E7392D

GO

Isoform 2 (identifier: Q9BUF7-2) [UniParc]FASTA Add to basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
101-120: VGARVPPTPNLKLPPEERLI → FSHAAEARAPQDSKETVQGCLPI

« Hide

        10         20         30         40         50
MANPGLGLLL ALGLPFLLAR WGRAWGQIQT TSANENSTVL PSSTSSSSDG 
        60         70         80         90        100
NLRPEAITAI IVVFSLLAAL LLAVGLALLV RKLREKRQTE GTYRPSSEEQ 
       110        120 
FSHAAEARAP QDSKETVQGC LPI

Show »

Length:123

Mass (Da):13,113

Checksum:ⁱD43DADF94F36303E

GO

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_013989	101 – 120	VGARV…EERLI → FSHAAEARAPQDSKETVQGC LPI in isoform 2. 2 PublicationsAdd BLAST		20

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY103469 mRNA Translation: AAM44074.1 AF503290 mRNA Translation: AAM23013.1 AY358684 mRNA Translation: AAQ89047.1 AK292956 mRNA Translation: BAF85645.1 CH471139 Genomic DNA Translation: EAW69084.1 CH471139 Genomic DNA Translation: EAW69085.1 CH471139 Genomic DNA Translation: EAW69086.1 BC002652 mRNA Translation: AAH02652.2 BC018409 mRNA Translation: AAH18409.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS12166.1 [Q9BUF7-2] CCDS12167.1 [Q9BUF7-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_631900.1, NM_139161.4 [Q9BUF7-1] NP_777377.1, NM_174881.3 [Q9BUF7-2] NP_777378.1, NM_174882.2 [Q9BUF7-1]
UniGeneⁱ	Hs.150319

Genome annotation databases

Ensemblⁱ	ENST00000308243; ENSP00000310123; ENSG00000130545 [Q9BUF7-1] ENST00000356762; ENSP00000349204; ENSG00000130545 [Q9BUF7-2] ENST00000598494; ENSP00000469707; ENSG00000130545 [Q9BUF7-1] ENST00000600229; ENSP00000472010; ENSG00000130545 [Q9BUF7-1]
GeneIDⁱ	92359
KEGGⁱ	hsa:92359
UCSC genome browser More...UCSCⁱ	uc002mey.4 human [Q9BUF7-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9BUF7	Crumbs protein homolog 3 isoform a		CALJA		124	UniRef90_Q9BUF7
CRB3 isoform 1		PONAB		122
CRB3 isoform 1		PANTR		120
UPI0006265702		AOTNA		122
Q9BUF7-2	CRB3 isoform 3		PANTR		123	UniRef90_Q9BUF7-2
UPI0007DC626D		HUMAN		186
UPI000D0A63D6		PANTR		186

Protein	Similar proteins		Species	Score	Length	Source
Q9BUF7	Crumbs protein homolog 3 isoform a		CALJA		124	UniRef50_Q9BUF7
CRB3 isoform 1		PONAB		122
CRB3 isoform 1		PANTR		120
Protein crumbs 3		CASCN		118
protein crumbs homolog 3 isoform X2		DIPOR		120
+124
Q9BUF7-2	CRB3 isoform 3		PANTR		123	UniRef50_Q9BUF7-2
Crumbs protein-like protein 3		HETGA		120
UPI0007DC626D		HUMAN		186
UPI000C813123		LOXAF		158
UPI000787B50B		ROUAE		192
+7

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY103469 mRNA Translation: AAM44074.1 AF503290 mRNA Translation: AAM23013.1 AY358684 mRNA Translation: AAQ89047.1 AK292956 mRNA Translation: BAF85645.1 CH471139 Genomic DNA Translation: EAW69084.1 CH471139 Genomic DNA Translation: EAW69085.1 CH471139 Genomic DNA Translation: EAW69086.1 BC002652 mRNA Translation: AAH02652.2 BC018409 mRNA Translation: AAH18409.1
CCDSⁱ	CCDS12166.1 [Q9BUF7-2] CCDS12167.1 [Q9BUF7-1]
RefSeqⁱ	NP_631900.1, NM_139161.4 [Q9BUF7-1] NP_777377.1, NM_174881.3 [Q9BUF7-2] NP_777378.1, NM_174882.2 [Q9BUF7-1]
UniGeneⁱ	Hs.150319

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5I7Z	X-ray	1.80	B	113-120	[»]
ProteinModelPortalⁱ	Q9BUF7
SMRⁱ	Q9BUF7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	124940, 3 interactors
IntActⁱ	Q9BUF7, 22 interactors
MINTⁱ	Q9BUF7

PTM databases

iPTMnetⁱ	Q9BUF7
PhosphoSitePlusⁱ	Q9BUF7

Polymorphism and mutation databases

BioMutaⁱ	CRB3
DMDMⁱ	67460584

Proteomic databases

EPDⁱ	Q9BUF7
MaxQBⁱ	Q9BUF7
PeptideAtlasⁱ	Q9BUF7
PRIDEⁱ	Q9BUF7
ProteomicsDBⁱ	79082 79083 [Q9BUF7-2]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000308243; ENSP00000310123; ENSG00000130545 [Q9BUF7-1] ENST00000356762; ENSP00000349204; ENSG00000130545 [Q9BUF7-2] ENST00000598494; ENSP00000469707; ENSG00000130545 [Q9BUF7-1] ENST00000600229; ENSP00000472010; ENSG00000130545 [Q9BUF7-1]
GeneIDⁱ	92359
KEGGⁱ	hsa:92359
UCSCⁱ	uc002mey.4 human [Q9BUF7-1]

Organism-specific databases

CTDⁱ	92359
DisGeNETⁱ	92359
EuPathDBⁱ	HostDB:ENSG00000130545.15
GeneCardsⁱ	CRB3
HGNCⁱ	HGNC:20237 CRB3
HPAⁱ	HPA013835
MIMⁱ	609737 gene
neXtProtⁱ	NX_Q9BUF7
OpenTargetsⁱ	ENSG00000130545
PharmGKBⁱ	PA134862130
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000012998
HOGENOMⁱ	HOG000013209
HOVERGENⁱ	HBG081347
InParanoidⁱ	Q9BUF7
KOⁱ	K06090
OMAⁱ	WGQVWGQ
OrthoDBⁱ	EOG091G14HT
PhylomeDBⁱ	Q9BUF7
TreeFamⁱ	TF353183

Enzyme and pathway databases

Reactomeⁱ	R-HSA-420029 Tight junction interactions
SIGNORⁱ	Q9BUF7

Miscellaneous databases

GeneWikiⁱ	CRB3
GenomeRNAiⁱ	92359
Protein Ontology More...PROⁱ	PR:Q9BUF7
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000130545 Expressed in 178 organ(s), highest expression level in lower esophagus mucosa
CleanExⁱ	HS_CRB3
Genevisibleⁱ	Q9BUF7 HS

Family and domain databases

ProtoNetⁱ	Search...

ProtoNetⁱ

Search...

Entry informationⁱ

Entry nameⁱ	CRUM3_HUMAN
Accessionⁱ	Q9BUF7Primary (citable) accession number: Q9BUF7 Secondary accession number(s): A8KA91 , D6W643, Q8N0V8, Q8WVA0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
	Last sequence update:	June 7, 2005
	Last modified:	September 12, 2018
	This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9BUF7 (CRUM3_HUMAN)

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Protein crumbs homolog 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Extracellular region or secreted

Plasma Membrane

Other locations

Topology

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

Keywords - Domaini

Phylogenomic databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ