UniProtKB - Q9BUF7 (CRUM3_HUMAN)
Protein crumbs homolog 3
CRB3
Functioni
GO - Molecular functioni
- protein domain specific binding Source: BHF-UCL
- SH3 domain binding Source: BHF-UCL
GO - Biological processi
- bicellular tight junction assembly Source: Reactome
- positive regulation of cell junction assembly Source: UniProtKB
- protein localization to plasma membrane Source: BHF-UCL
Enzyme and pathway databases
PathwayCommonsi | Q9BUF7 |
Reactomei | R-HSA-420029, Tight junction interactions |
SIGNORi | Q9BUF7 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein crumbs homolog 3 |
Gene namesi | Name:CRB3Imported ORF Names:UNQ588/PRO1158 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20237, CRB3 |
MIMi | 609737, gene |
neXtProti | NX_Q9BUF7 |
VEuPathDBi | HostDB:ENSG00000130545.15 |
Subcellular locationi
Plasma membrane
- Apical cell membrane 3 Publications; Single-pass type I membrane protein Sequence analysis
Other locations
- tight junction 1 Publication
Note: Localizes primarily to the apical membrane with a small fraction in the upper part of tight junctions of epithelial cells.1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- plasma membrane Source: Reactome
Other locations
- bicellular tight junction Source: UniProtKB-SubCell
- cell junction Source: HPA
- integral component of membrane Source: UniProtKB-KW
- protein-containing complex Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 27 – 59 | ExtracellularSequence analysisAdd BLAST | 33 | |
Transmembranei | 60 – 80 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 81 – 120 | CytoplasmicSequence analysisAdd BLAST | 40 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | N → D: Abolishes N-glycosylation. No effect on localization to the apical cell membrane. 1 Publication | 1 | |
Mutagenesisi | 117 – 120 | Missing : Loss of interaction with PARD6A and MPP5. No effect on interaction with EPB41L5. 3 Publications | 4 |
Organism-specific databases
DisGeNETi | 92359 |
OpenTargetsi | ENSG00000130545 |
PharmGKBi | PA134862130 |
Miscellaneous databases
Pharosi | Q9BUF7, Tbio |
Genetic variation databases
BioMutai | CRB3 |
DMDMi | 67460584 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Sequence analysisAdd BLAST | 26 | |
ChainiPRO_0000021005 | 27 – 120 | Protein crumbs homolog 3Add BLAST | 94 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 36 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q9BUF7 |
jPOSTi | Q9BUF7 |
MassIVEi | Q9BUF7 |
MaxQBi | Q9BUF7 |
PeptideAtlasi | Q9BUF7 |
PRIDEi | Q9BUF7 |
ProteomicsDBi | 79082 [Q9BUF7-1] 79083 [Q9BUF7-2] |
PTM databases
GlyGeni | Q9BUF7, 1 site |
iPTMneti | Q9BUF7 |
PhosphoSitePlusi | Q9BUF7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000130545, Expressed in lower esophagus mucosa and 184 other tissues |
Genevisiblei | Q9BUF7, HS |
Organism-specific databases
HPAi | ENSG00000130545, Low tissue specificity |
Interactioni
Subunit structurei
Component of a complex composed of CRB3, MPP5/PALS1 and PATJ (By similarity).
Interacts (via C-terminus) with MPP5/PALS1 (via PDZ domain) (PubMed:12527193, PubMed:12771187).
Interacts with PARD6A (PubMed:14718572).
Interacts (via intracellular domain) with EPB41L5 (PubMed:17920587).
By similarity4 PublicationsBinary interactionsi
Hide detailsQ9BUF7
With | #Exp. | IntAct |
---|---|---|
PSMA3 [P25788] | 3 | EBI-9844372,EBI-348380 |
Isoform 2 [Q9BUF7-2]
Protein crumbs homolog 3 (PRO_0000021005)
With | #Exp. | IntAct |
---|---|---|
MPP5 - isoform 1 [Q8N3R9-1] | 3 | EBI-25611611,EBI-8231026 |
GO - Molecular functioni
- protein domain specific binding Source: BHF-UCL
- SH3 domain binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 124940, 28 interactors |
ComplexPortali | CPX-6166, CRUMBS3-PALS1-PATJ cell polarity complex |
IntActi | Q9BUF7, 26 interactors |
MINTi | Q9BUF7 |
STRINGi | 9606.ENSP00000349204 |
Miscellaneous databases
RNActi | Q9BUF7, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9BUF7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 84 – 120 | Interaction with EPB41L51 PublicationAdd BLAST | 37 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 117 – 120 | PDZ-binding | 4 |
Domaini
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S74B, Eukaryota |
GeneTreei | ENSGT00950000183101 |
HOGENOMi | CLU_2120342_0_0_1 |
InParanoidi | Q9BUF7 |
OMAi | WGQVWGQ |
OrthoDBi | 1551105at2759 |
PhylomeDBi | Q9BUF7 |
TreeFami | TF353183 |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MANPGLGLLL ALGLPFLLAR WGRAWGQIQT TSANENSTVL PSSTSSSSDG
60 70 80 90 100
NLRPEAITAI IVVFSLLAAL LLAVGLALLV RKLREKRQTE GTYRPSSEEQ
110 120
VGARVPPTPN LKLPPEERLI
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013989 | 101 – 120 | VGARV…EERLI → FSHAAEARAPQDSKETVQGC LPI in isoform 2. 2 PublicationsAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY103469 mRNA Translation: AAM44074.1 AF503290 mRNA Translation: AAM23013.1 AY358684 mRNA Translation: AAQ89047.1 AK292956 mRNA Translation: BAF85645.1 CH471139 Genomic DNA Translation: EAW69084.1 CH471139 Genomic DNA Translation: EAW69085.1 CH471139 Genomic DNA Translation: EAW69086.1 BC002652 mRNA Translation: AAH02652.2 BC018409 mRNA Translation: AAH18409.1 |
CCDSi | CCDS12166.1 [Q9BUF7-2] CCDS12167.1 [Q9BUF7-1] |
RefSeqi | NP_631900.1, NM_139161.4 [Q9BUF7-1] NP_777377.1, NM_174881.3 [Q9BUF7-2] NP_777378.1, NM_174882.2 [Q9BUF7-1] |
Genome annotation databases
Ensembli | ENST00000308243; ENSP00000310123; ENSG00000130545 [Q9BUF7-1] ENST00000356762; ENSP00000349204; ENSG00000130545 [Q9BUF7-2] ENST00000598494; ENSP00000469707; ENSG00000130545 [Q9BUF7-1] ENST00000600229; ENSP00000472010; ENSG00000130545 [Q9BUF7-1] |
GeneIDi | 92359 |
KEGGi | hsa:92359 |
UCSCi | uc002mey.4, human [Q9BUF7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY103469 mRNA Translation: AAM44074.1 AF503290 mRNA Translation: AAM23013.1 AY358684 mRNA Translation: AAQ89047.1 AK292956 mRNA Translation: BAF85645.1 CH471139 Genomic DNA Translation: EAW69084.1 CH471139 Genomic DNA Translation: EAW69085.1 CH471139 Genomic DNA Translation: EAW69086.1 BC002652 mRNA Translation: AAH02652.2 BC018409 mRNA Translation: AAH18409.1 |
CCDSi | CCDS12166.1 [Q9BUF7-2] CCDS12167.1 [Q9BUF7-1] |
RefSeqi | NP_631900.1, NM_139161.4 [Q9BUF7-1] NP_777377.1, NM_174881.3 [Q9BUF7-2] NP_777378.1, NM_174882.2 [Q9BUF7-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5I7Z | X-ray | 1.80 | B | 113-120 | [»] | |
SMRi | Q9BUF7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124940, 28 interactors |
ComplexPortali | CPX-6166, CRUMBS3-PALS1-PATJ cell polarity complex |
IntActi | Q9BUF7, 26 interactors |
MINTi | Q9BUF7 |
STRINGi | 9606.ENSP00000349204 |
PTM databases
GlyGeni | Q9BUF7, 1 site |
iPTMneti | Q9BUF7 |
PhosphoSitePlusi | Q9BUF7 |
Genetic variation databases
BioMutai | CRB3 |
DMDMi | 67460584 |
Proteomic databases
EPDi | Q9BUF7 |
jPOSTi | Q9BUF7 |
MassIVEi | Q9BUF7 |
MaxQBi | Q9BUF7 |
PeptideAtlasi | Q9BUF7 |
PRIDEi | Q9BUF7 |
ProteomicsDBi | 79082 [Q9BUF7-1] 79083 [Q9BUF7-2] |
Protocols and materials databases
Antibodypediai | 3003, 100 antibodies |
Genome annotation databases
Ensembli | ENST00000308243; ENSP00000310123; ENSG00000130545 [Q9BUF7-1] ENST00000356762; ENSP00000349204; ENSG00000130545 [Q9BUF7-2] ENST00000598494; ENSP00000469707; ENSG00000130545 [Q9BUF7-1] ENST00000600229; ENSP00000472010; ENSG00000130545 [Q9BUF7-1] |
GeneIDi | 92359 |
KEGGi | hsa:92359 |
UCSCi | uc002mey.4, human [Q9BUF7-1] |
Organism-specific databases
CTDi | 92359 |
DisGeNETi | 92359 |
GeneCardsi | CRB3 |
HGNCi | HGNC:20237, CRB3 |
HPAi | ENSG00000130545, Low tissue specificity |
MIMi | 609737, gene |
neXtProti | NX_Q9BUF7 |
OpenTargetsi | ENSG00000130545 |
PharmGKBi | PA134862130 |
VEuPathDBi | HostDB:ENSG00000130545.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S74B, Eukaryota |
GeneTreei | ENSGT00950000183101 |
HOGENOMi | CLU_2120342_0_0_1 |
InParanoidi | Q9BUF7 |
OMAi | WGQVWGQ |
OrthoDBi | 1551105at2759 |
PhylomeDBi | Q9BUF7 |
TreeFami | TF353183 |
Enzyme and pathway databases
PathwayCommonsi | Q9BUF7 |
Reactomei | R-HSA-420029, Tight junction interactions |
SIGNORi | Q9BUF7 |
Miscellaneous databases
BioGRID-ORCSi | 92359, 7 hits in 871 CRISPR screens |
ChiTaRSi | CRB3, human |
GeneWikii | CRB3 |
GenomeRNAii | 92359 |
Pharosi | Q9BUF7, Tbio |
PROi | PR:Q9BUF7 |
RNActi | Q9BUF7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000130545, Expressed in lower esophagus mucosa and 184 other tissues |
Genevisiblei | Q9BUF7, HS |
Family and domain databases
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CRUM3_HUMAN | |
Accessioni | Q9BUF7Primary (citable) accession number: Q9BUF7 Secondary accession number(s): A8KA91 Q8WVA0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references