UniProtKB - Q9BUB5 (MKNK1_HUMAN)
Protein
MAP kinase-interacting serine/threonine-protein kinase 1
Gene
MKNK1
Organism
Homo sapiens (Human)
Status
Functioni
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.4 Publications
Catalytic activityi
Cofactori
Mg2+2 Publications
Activity regulationi
Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 78 | ATPPROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 211 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 55 – 63 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- calcium-dependent protein serine/threonine kinase activity Source: GO_Central
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein kinase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- intracellular signal transduction Source: UniProtKB
- peptidyl-serine phosphorylation Source: BHF-UCL
- protein autophosphorylation Source: GO_Central
- protein phosphorylation Source: UniProtKB
- regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Translation regulation |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9BUB5 |
Reactomei | R-HSA-1295596, Spry regulation of FGF signaling |
SignaLinki | Q9BUB5 |
SIGNORi | Q9BUB5 |
Names & Taxonomyi
Protein namesi | Recommended name: MAP kinase-interacting serine/threonine-protein kinase 1 (EC:2.7.11.12 Publications)Alternative name(s): MAP kinase signal-integrating kinase 1 Short name: MAPK signal-integrating kinase 1 Short name: Mnk1 |
Gene namesi | Name:MKNK1 Synonyms:MNK1 |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7110, MKNK1 |
MIMi | 606724, gene |
neXtProti | NX_Q9BUB5 |
VEuPathDBi | HostDB:ENSG00000079277.19 |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | K → M: Loss of kinase activity; when associated with D-232. 1 Publication | 1 | |
Mutagenesisi | 232 | D → A: Loss of kinase activity; when associated with K-78. 1 Publication | 1 | |
Mutagenesisi | 250 | T → A: Loss of kinase activity; when associated with T-255. 1 Publication | 1 | |
Mutagenesisi | 255 | T → A: Loss of kinase activity; when associated with T-250. 1 Publication | 1 | |
Mutagenesisi | 385 | T → D: Constitutively active. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 8569 |
OpenTargetsi | ENSG00000079277 |
PharmGKBi | PA30829 |
Miscellaneous databases
Pharosi | Q9BUB5, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4718 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q9BUB5 |
GuidetoPHARMACOLOGYi | 2104 |
Genetic variation databases
BioMutai | MKNK1 |
DMDMi | 30316115 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086334 | 1 – 465 | MAP kinase-interacting serine/threonine-protein kinase 1Add BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 39 | PhosphoserineCombined sources | 1 | |
Modified residuei | 221 | PhosphoserineCombined sources | 1 | |
Modified residuei | 226 | PhosphoserineCombined sources | 1 | |
Modified residuei | 250 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 255 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 385 | PhosphothreonineCombined sources1 Publication | 1 | |
Modified residuei | 460 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.4 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9BUB5 |
jPOSTi | Q9BUB5 |
MassIVEi | Q9BUB5 |
MaxQBi | Q9BUB5 |
PaxDbi | Q9BUB5 |
PeptideAtlasi | Q9BUB5 |
PRIDEi | Q9BUB5 |
ProteomicsDBi | 79072 [Q9BUB5-1] 79073 [Q9BUB5-2] 79074 [Q9BUB5-3] |
PTM databases
iPTMneti | Q9BUB5 |
MetOSitei | Q9BUB5 |
PhosphoSitePlusi | Q9BUB5 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000079277, Expressed in body of pancreas and 236 other tissues |
ExpressionAtlasi | Q9BUB5, baseline and differential |
Genevisiblei | Q9BUB5, HS |
Organism-specific databases
HPAi | ENSG00000079277, Tissue enhanced (pancreas) |
Interactioni
Subunit structurei
Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.
1 PublicationBinary interactionsi
Hide detailsQ9BUB5
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 114138, 51 interactors |
CORUMi | Q9BUB5 |
ELMi | Q9BUB5 |
IntActi | Q9BUB5, 49 interactors |
MINTi | Q9BUB5 |
STRINGi | 9606.ENSP00000361014 |
Chemistry databases
BindingDBi | Q9BUB5 |
Miscellaneous databases
RNActi | Q9BUB5, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9BUB5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9BUB5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 49 – 374 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 326 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0607, Eukaryota |
GeneTreei | ENSGT00940000162886 |
InParanoidi | Q9BUB5 |
OMAi | WERGETC |
OrthoDBi | 669799at2759 |
PhylomeDBi | Q9BUB5 |
TreeFami | TF314050 |
Family and domain databases
IDEALi | IID00679 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 13 potential isoforms that are computationally mapped.Show allAlign All
Isoform 11 Publication (identifier: Q9BUB5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVSSQKLEKP IEMGSSEPLP IADGDRRRKK KRRGRATDSL PGKFEDMYKL
60 70 80 90 100
TSELLGEGAY AKVQGAVSLQ NGKEYAVKII EKQAGHSRSR VFREVETLYQ
110 120 130 140 150
CQGNKNILEL IEFFEDDTRF YLVFEKLQGG SILAHIQKQK HFNEREASRV
160 170 180 190 200
VRDVAAALDF LHTKDKVSLC HLGWSAMAPS GLTAAPTSLG SSDPPTSASQ
210 220 230 240 250
VAGTTGIAHR DLKPENILCE SPEKVSPVKI CDFDLGSGMK LNNSCTPITT
260 270 280 290 300
PELTTPCGSA EYMAPEVVEV FTDQATFYDK RCDLWSLGVV LYIMLSGYPP
310 320 330 340 350
FVGHCGADCG WDRGEVCRVC QNKLFESIQE GKYEFPDKDW AHISSEAKDL
360 370 380 390 400
ISKLLVRDAK QRLSAAQVLQ HPWVQGQAPE KGLPTPQVLQ RNSSTMDLTL
410 420 430 440 450
FAAEAIALNR QLSQHEENEL AEEPEALADG LCSMKLSPPC KSRLARRRAL
460
AQAGRGEDRS PPTAL
Computationally mapped potential isoform sequencesi
There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PIA0 | E9PIA0_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 166 | Annotation score: | ||
A0A499FJN1 | A0A499FJN1_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 412 | Annotation score: | ||
A0A499FIS5 | A0A499FIS5_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 453 | Annotation score: | ||
A0A499FJS1 | A0A499FJS1_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 335 | Annotation score: | ||
E9PMM8 | E9PMM8_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 106 | Annotation score: | ||
E9PSE0 | E9PSE0_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 88 | Annotation score: | ||
Q7Z319 | Q7Z319_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 DKFZp686E14208 | 329 | Annotation score: | ||
E9PLE7 | E9PLE7_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 89 | Annotation score: | ||
E9PN34 | E9PN34_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 79 | Annotation score: | ||
E9PQ26 | E9PQ26_HUMAN | MAP kinase-interacting serine/threo... | MKNK1 | 44 | Annotation score: | ||
There are more potential isoformsShow all |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_040801 | 49 | K → Q1 PublicationCorresponds to variant dbSNP:rs56351860Ensembl. | 1 | |
Natural variantiVAR_040802 | 158 | L → V1 PublicationCorresponds to variant dbSNP:rs56408722Ensembl. | 1 | |
Natural variantiVAR_040803 | 308 | D → N1 PublicationCorresponds to variant dbSNP:rs55791614Ensembl. | 1 | |
Natural variantiVAR_040804 | 446 | R → Q1 PublicationCorresponds to variant dbSNP:rs34881418Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007352 | 165 – 205 | Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST | 41 | |
Alternative sequenceiVSP_017515 | 377 – 465 | QAPEK…PPTAL → EQQHNGPDALRS in isoform 3. 1 PublicationAdd BLAST | 89 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000409 mRNA Translation: BAA19885.1 AY355461 mRNA Translation: AAQ84219.1 AL136373 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06900.1 CH471059 Genomic DNA Translation: EAX06902.1 CH471059 Genomic DNA Translation: EAX06904.1 CH471059 Genomic DNA Translation: EAX06905.1 BC002755 mRNA Translation: AAH02755.1 |
RefSeqi | NP_001129025.1, NM_001135553.2 NP_003675.2, NM_003684.5 NP_945324.1, NM_198973.3 XP_006711063.1, XM_006711000.1 XP_016858148.1, XM_017002659.1 |
Genome annotation databases
Ensembli | ENST00000649800; ENSP00000498083; ENSG00000079277 [Q9BUB5-1] ENST00000650026; ENSP00000497380; ENSG00000079277 [Q9BUB5-3] ENST00000650508; ENSP00000498143; ENSG00000079277 [Q9BUB5-2] |
GeneIDi | 8569 |
KEGGi | hsa:8569 |
UCSCi | uc001cqb.5, human [Q9BUB5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000409 mRNA Translation: BAA19885.1 AY355461 mRNA Translation: AAQ84219.1 AL136373 Genomic DNA No translation available. CH471059 Genomic DNA Translation: EAX06900.1 CH471059 Genomic DNA Translation: EAX06902.1 CH471059 Genomic DNA Translation: EAX06904.1 CH471059 Genomic DNA Translation: EAX06905.1 BC002755 mRNA Translation: AAH02755.1 |
RefSeqi | NP_001129025.1, NM_001135553.2 NP_003675.2, NM_003684.5 NP_945324.1, NM_198973.3 XP_006711063.1, XM_006711000.1 XP_016858148.1, XM_017002659.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2HW6 | X-ray | 2.50 | A/B | 37-382 | [»] | |
2Y9Q | X-ray | 1.55 | B | 434-451 | [»] | |
5WVD | X-ray | 3.00 | A/B | 37-382 | [»] | |
SMRi | Q9BUB5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114138, 51 interactors |
CORUMi | Q9BUB5 |
ELMi | Q9BUB5 |
IntActi | Q9BUB5, 49 interactors |
MINTi | Q9BUB5 |
STRINGi | 9606.ENSP00000361014 |
Chemistry databases
BindingDBi | Q9BUB5 |
ChEMBLi | CHEMBL4718 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q9BUB5 |
GuidetoPHARMACOLOGYi | 2104 |
PTM databases
iPTMneti | Q9BUB5 |
MetOSitei | Q9BUB5 |
PhosphoSitePlusi | Q9BUB5 |
Genetic variation databases
BioMutai | MKNK1 |
DMDMi | 30316115 |
Proteomic databases
EPDi | Q9BUB5 |
jPOSTi | Q9BUB5 |
MassIVEi | Q9BUB5 |
MaxQBi | Q9BUB5 |
PaxDbi | Q9BUB5 |
PeptideAtlasi | Q9BUB5 |
PRIDEi | Q9BUB5 |
ProteomicsDBi | 79072 [Q9BUB5-1] 79073 [Q9BUB5-2] 79074 [Q9BUB5-3] |
Protocols and materials databases
Antibodypediai | 32808, 556 antibodies |
DNASUi | 8569 |
Genome annotation databases
Ensembli | ENST00000649800; ENSP00000498083; ENSG00000079277 [Q9BUB5-1] ENST00000650026; ENSP00000497380; ENSG00000079277 [Q9BUB5-3] ENST00000650508; ENSP00000498143; ENSG00000079277 [Q9BUB5-2] |
GeneIDi | 8569 |
KEGGi | hsa:8569 |
UCSCi | uc001cqb.5, human [Q9BUB5-1] |
Organism-specific databases
CTDi | 8569 |
DisGeNETi | 8569 |
GeneCardsi | MKNK1 |
HGNCi | HGNC:7110, MKNK1 |
HPAi | ENSG00000079277, Tissue enhanced (pancreas) |
MIMi | 606724, gene |
neXtProti | NX_Q9BUB5 |
OpenTargetsi | ENSG00000079277 |
PharmGKBi | PA30829 |
VEuPathDBi | HostDB:ENSG00000079277.19 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0607, Eukaryota |
GeneTreei | ENSGT00940000162886 |
InParanoidi | Q9BUB5 |
OMAi | WERGETC |
OrthoDBi | 669799at2759 |
PhylomeDBi | Q9BUB5 |
TreeFami | TF314050 |
Enzyme and pathway databases
PathwayCommonsi | Q9BUB5 |
Reactomei | R-HSA-1295596, Spry regulation of FGF signaling |
SignaLinki | Q9BUB5 |
SIGNORi | Q9BUB5 |
Miscellaneous databases
BioGRID-ORCSi | 8569, 135 hits in 1028 CRISPR screens |
ChiTaRSi | MKNK1, human |
EvolutionaryTracei | Q9BUB5 |
GeneWikii | MKNK1 |
GenomeRNAii | 8569 |
Pharosi | Q9BUB5, Tchem |
PROi | PR:Q9BUB5 |
RNActi | Q9BUB5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000079277, Expressed in body of pancreas and 236 other tissues |
ExpressionAtlasi | Q9BUB5, baseline and differential |
Genevisiblei | Q9BUB5, HS |
Family and domain databases
IDEALi | IID00679 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MKNK1_HUMAN | |
Accessioni | Q9BUB5Primary (citable) accession number: Q9BUB5 Secondary accession number(s): D3DQ20 Q6V0N6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 30, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 192 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families