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Protein

MAP kinase-interacting serine/threonine-protein kinase 1

Gene

MKNK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+2 Publications

Activity regulationi

Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78ATPPROSITE-ProRule annotation1 Publication1
Active sitei211Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 63ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processTranslation regulation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1295596 Spry regulation of FGF signaling
SignaLinkiQ9BUB5
SIGNORiQ9BUB5

Names & Taxonomyi

Protein namesi
Recommended name:
MAP kinase-interacting serine/threonine-protein kinase 1 (EC:2.7.11.12 Publications)
Alternative name(s):
MAP kinase signal-integrating kinase 1
Short name:
MAPK signal-integrating kinase 1
Short name:
Mnk1
Gene namesi
Name:MKNK1
Synonyms:MNK1
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000079277.19
HGNCiHGNC:7110 MKNK1
MIMi606724 gene
neXtProtiNX_Q9BUB5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78K → M: Loss of kinase activity; when associated with D-232. 1 Publication1
Mutagenesisi232D → A: Loss of kinase activity; when associated with K-78. 1 Publication1
Mutagenesisi250T → A: Loss of kinase activity; when associated with T-255. 1 Publication1
Mutagenesisi255T → A: Loss of kinase activity; when associated with T-250. 1 Publication1
Mutagenesisi385T → D: Constitutively active. 1 Publication1

Organism-specific databases

DisGeNETi8569
OpenTargetsiENSG00000079277
PharmGKBiPA30829

Chemistry databases

ChEMBLiCHEMBL4718
GuidetoPHARMACOLOGYi2104

Polymorphism and mutation databases

BioMutaiMKNK1
DMDMi30316115

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863341 – 465MAP kinase-interacting serine/threonine-protein kinase 1Add BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei226PhosphoserineCombined sources1
Modified residuei250Phosphothreonine1 Publication1
Modified residuei255Phosphothreonine1 Publication1
Modified residuei385PhosphothreonineCombined sources1 Publication1
Modified residuei460PhosphoserineCombined sources1

Post-translational modificationi

Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BUB5
MaxQBiQ9BUB5
PaxDbiQ9BUB5
PeptideAtlasiQ9BUB5
PRIDEiQ9BUB5
ProteomicsDBi79072
79073 [Q9BUB5-2]
79074 [Q9BUB5-3]

PTM databases

iPTMnetiQ9BUB5
PhosphoSitePlusiQ9BUB5

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000079277 Expressed in 225 organ(s), highest expression level in body of pancreas
CleanExiHS_MKNK1
ExpressionAtlasiQ9BUB5 baseline and differential
GenevisibleiQ9BUB5 HS

Organism-specific databases

HPAiCAB027355
CAB037310
HPA071293

Interactioni

Subunit structurei

Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114138, 49 interactors
CORUMiQ9BUB5
ELMiQ9BUB5
IntActiQ9BUB5, 43 interactors
MINTiQ9BUB5
STRINGi9606.ENSP00000361014

Chemistry databases

BindingDBiQ9BUB5

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BUB5
SMRiQ9BUB5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BUB5

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 374Protein kinasePROSITE-ProRule annotationAdd BLAST326

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0607 Eukaryota
ENOG410XQA9 LUCA
GeneTreeiENSGT00900000140876
HOVERGENiHBG106949
InParanoidiQ9BUB5
KOiK04372
OMAiEWRCISE
OrthoDBiEOG091G0G1X
PhylomeDBiQ9BUB5
TreeFamiTF314050

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 11 Publication (identifier: Q9BUB5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVSSQKLEKP IEMGSSEPLP IADGDRRRKK KRRGRATDSL PGKFEDMYKL
60 70 80 90 100
TSELLGEGAY AKVQGAVSLQ NGKEYAVKII EKQAGHSRSR VFREVETLYQ
110 120 130 140 150
CQGNKNILEL IEFFEDDTRF YLVFEKLQGG SILAHIQKQK HFNEREASRV
160 170 180 190 200
VRDVAAALDF LHTKDKVSLC HLGWSAMAPS GLTAAPTSLG SSDPPTSASQ
210 220 230 240 250
VAGTTGIAHR DLKPENILCE SPEKVSPVKI CDFDLGSGMK LNNSCTPITT
260 270 280 290 300
PELTTPCGSA EYMAPEVVEV FTDQATFYDK RCDLWSLGVV LYIMLSGYPP
310 320 330 340 350
FVGHCGADCG WDRGEVCRVC QNKLFESIQE GKYEFPDKDW AHISSEAKDL
360 370 380 390 400
ISKLLVRDAK QRLSAAQVLQ HPWVQGQAPE KGLPTPQVLQ RNSSTMDLTL
410 420 430 440 450
FAAEAIALNR QLSQHEENEL AEEPEALADG LCSMKLSPPC KSRLARRRAL
460
AQAGRGEDRS PPTAL
Length:465
Mass (Da):51,342
Last modified:June 1, 2001 - v1
Checksum:iCAE225C35DCB2B43
GO
Isoform 21 Publication (identifier: Q9BUB5-2) [UniParc]FASTAAdd to basket
Also known as: MNK1a

The sequence of this isoform differs from the canonical sequence as follows:
     165-205: Missing.

Show »
Length:424
Mass (Da):47,402
Checksum:iDB39E49EC0BED990
GO
Isoform 3 (identifier: Q9BUB5-3) [UniParc]FASTAAdd to basket
Also known as: MNK1b

The sequence of this isoform differs from the canonical sequence as follows:
     165-205: Missing.
     377-465: QAPEKGLPTP...GEDRSPPTAL → EQQHNGPDALRS

Show »
Length:347
Mass (Da):39,104
Checksum:i1CCA03B12D39641F
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PIA0E9PIA0_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
178Annotation score:
Q7Z319Q7Z319_HUMAN
MAP kinase-interacting serine/threo...
MKNK1 DKFZp686E14208
329Annotation score:
E9PMM8E9PMM8_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
118Annotation score:
E9PSE0E9PSE0_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
100Annotation score:
H0YE67H0YE67_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
85Annotation score:
E9PLE7E9PLE7_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
101Annotation score:
E9PN34E9PN34_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
91Annotation score:
E9PII5E9PII5_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
126Annotation score:
E9PQ26E9PQ26_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
56Annotation score:
E9PSC9E9PSC9_HUMAN
MAP kinase-interacting serine/threo...
MKNK1
60Annotation score:
There is more potential isoformShow all

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04080149K → Q1 PublicationCorresponds to variant dbSNP:rs56351860Ensembl.1
Natural variantiVAR_040802158L → V1 PublicationCorresponds to variant dbSNP:rs56408722Ensembl.1
Natural variantiVAR_040803308D → N1 PublicationCorresponds to variant dbSNP:rs55791614Ensembl.1
Natural variantiVAR_040804446R → Q1 PublicationCorresponds to variant dbSNP:rs34881418Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007352165 – 205Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST41
Alternative sequenceiVSP_017515377 – 465QAPEK…PPTAL → EQQHNGPDALRS in isoform 3. 1 PublicationAdd BLAST89

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000409 mRNA Translation: BAA19885.1
AY355461 mRNA Translation: AAQ84219.1
AL136373 Genomic DNA No translation available.
CH471059 Genomic DNA Translation: EAX06900.1
CH471059 Genomic DNA Translation: EAX06902.1
CH471059 Genomic DNA Translation: EAX06904.1
CH471059 Genomic DNA Translation: EAX06905.1
BC002755 mRNA Translation: AAH02755.1
RefSeqiNP_001129025.1, NM_001135553.2
NP_003675.2, NM_003684.5
NP_945324.1, NM_198973.3
XP_006711063.1, XM_006711000.1 [Q9BUB5-2]
XP_016858148.1, XM_017002659.1 [Q9BUB5-3]
UniGeneiHs.371594

Genome annotation databases

EnsembliENST00000371945; ENSP00000361013; ENSG00000079277 [Q9BUB5-2]
ENST00000371946; ENSP00000361014; ENSG00000079277 [Q9BUB5-1]
ENST00000428112; ENSP00000411135; ENSG00000079277 [Q9BUB5-3]
GeneIDi8569
KEGGihsa:8569
UCSCiuc001cqb.5 human [Q9BUB5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000409 mRNA Translation: BAA19885.1
AY355461 mRNA Translation: AAQ84219.1
AL136373 Genomic DNA No translation available.
CH471059 Genomic DNA Translation: EAX06900.1
CH471059 Genomic DNA Translation: EAX06902.1
CH471059 Genomic DNA Translation: EAX06904.1
CH471059 Genomic DNA Translation: EAX06905.1
BC002755 mRNA Translation: AAH02755.1
RefSeqiNP_001129025.1, NM_001135553.2
NP_003675.2, NM_003684.5
NP_945324.1, NM_198973.3
XP_006711063.1, XM_006711000.1 [Q9BUB5-2]
XP_016858148.1, XM_017002659.1 [Q9BUB5-3]
UniGeneiHs.371594

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HW6X-ray2.50A/B37-382[»]
2Y9QX-ray1.55B434-451[»]
5WVDX-ray3.00A/B37-382[»]
ProteinModelPortaliQ9BUB5
SMRiQ9BUB5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114138, 49 interactors
CORUMiQ9BUB5
ELMiQ9BUB5
IntActiQ9BUB5, 43 interactors
MINTiQ9BUB5
STRINGi9606.ENSP00000361014

Chemistry databases

BindingDBiQ9BUB5
ChEMBLiCHEMBL4718
GuidetoPHARMACOLOGYi2104

PTM databases

iPTMnetiQ9BUB5
PhosphoSitePlusiQ9BUB5

Polymorphism and mutation databases

BioMutaiMKNK1
DMDMi30316115

Proteomic databases

EPDiQ9BUB5
MaxQBiQ9BUB5
PaxDbiQ9BUB5
PeptideAtlasiQ9BUB5
PRIDEiQ9BUB5
ProteomicsDBi79072
79073 [Q9BUB5-2]
79074 [Q9BUB5-3]

Protocols and materials databases

DNASUi8569
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371945; ENSP00000361013; ENSG00000079277 [Q9BUB5-2]
ENST00000371946; ENSP00000361014; ENSG00000079277 [Q9BUB5-1]
ENST00000428112; ENSP00000411135; ENSG00000079277 [Q9BUB5-3]
GeneIDi8569
KEGGihsa:8569
UCSCiuc001cqb.5 human [Q9BUB5-1]

Organism-specific databases

CTDi8569
DisGeNETi8569
EuPathDBiHostDB:ENSG00000079277.19
GeneCardsiMKNK1
HGNCiHGNC:7110 MKNK1
HPAiCAB027355
CAB037310
HPA071293
MIMi606724 gene
neXtProtiNX_Q9BUB5
OpenTargetsiENSG00000079277
PharmGKBiPA30829
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0607 Eukaryota
ENOG410XQA9 LUCA
GeneTreeiENSGT00900000140876
HOVERGENiHBG106949
InParanoidiQ9BUB5
KOiK04372
OMAiEWRCISE
OrthoDBiEOG091G0G1X
PhylomeDBiQ9BUB5
TreeFamiTF314050

Enzyme and pathway databases

ReactomeiR-HSA-1295596 Spry regulation of FGF signaling
SignaLinkiQ9BUB5
SIGNORiQ9BUB5

Miscellaneous databases

ChiTaRSiMKNK1 human
EvolutionaryTraceiQ9BUB5
GeneWikiiMKNK1
GenomeRNAii8569
PROiPR:Q9BUB5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000079277 Expressed in 225 organ(s), highest expression level in body of pancreas
CleanExiHS_MKNK1
ExpressionAtlasiQ9BUB5 baseline and differential
GenevisibleiQ9BUB5 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 2 hits
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMKNK1_HUMAN
AccessioniPrimary (citable) accession number: Q9BUB5
Secondary accession number(s): D3DQ20
, D3DQ21, O00312, Q5TC06, Q5TC07, Q6V0N6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  7. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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