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Protein

Deoxyhypusine hydroxylase

Gene

DOHH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine (PubMed:16533814, PubMed:16371467, PubMed:19706422). Hypusination is unique to mature eIF-5A factor and is essential for its function (By similarity).UniRule annotationBy similarity3 Publications

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation3 Publications

Cofactori

Fe2+UniRule annotationCombined sources3 PublicationsNote: Binds 2 Fe2+ ions per subunit.UniRule annotationCombined sources3 Publications

pH dependencei

Optimum pH is 8-8.5 at 37 degrees Celsius.1 Publication

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation3 Publications
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Iron 1UniRule annotationCombined sources2 Publications1
Metal bindingi89Iron 2UniRule annotationCombined sources2 Publications1
Metal bindingi90Iron 2UniRule annotationCombined sources2 Publications1
Metal bindingi207Iron 2UniRule annotationCombined sources2 Publications1
Metal bindingi240Iron 1UniRule annotationCombined sources2 Publications1
Metal bindingi241Iron 1UniRule annotationCombined sources2 Publications1

GO - Molecular functioni

  • cofactor binding Source: UniProtKB
  • deoxyhypusine monooxygenase activity Source: UniProtKB
  • iron ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processHypusine biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05318-MONOMER
BRENDAi1.14.99.29 2681
ReactomeiR-HSA-204626 Hypusine synthesis from eIF5A-lysine
UniPathwayi
UPA00354

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylase1 PublicationUniRule annotation (EC:1.14.99.29UniRule annotation3 Publications)
Short name:
hDOHH1 Publication
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
HEAT-like repeat-containing protein 11 Publication
Gene namesi
Name:DOHHUniRule annotationImported
Synonyms:HLRC11 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000129932.7
HGNCiHGNC:28662 DOHH
MIMi611262 gene
neXtProtiNX_Q9BU89

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56H → A: Loss of deoxyhypusine monooxygenase activity. Loss of iron-binding. 1 Publication1
Mutagenesisi57E → A: Loss of deoxyhypusine monooxygenase activity. No effect on iron-binding. 1 Publication1
Mutagenesisi86M → A: Loss of iron-binding. 1 Publication1
Mutagenesisi86M → L: No effect on iron-binding. Loss of iron-binding; when associated with L-237. 1 Publication1
Mutagenesisi89H → A: Loss of deoxyhypusine monooxygenase activity. Loss of iron-binding. 1 Publication1
Mutagenesisi90E → A: Loss of deoxyhypusine monooxygenase activity. Loss of iron-binding. 1 Publication1
Mutagenesisi207H → A: Loss of deoxyhypusine monooxygenase activity. Loss of iron-binding. 1 Publication1
Mutagenesisi208E → A: Loss of deoxyhypusine monooxygenase activity. No effect on iron-binding. 1 Publication1
Mutagenesisi237M → L: Decreased iron-binding. Loss of iron-binding; when associated with L-86. 1 Publication1
Mutagenesisi240H → A: Loss of deoxyhypusine monooxygenase activity. Loss of iron-binding. 1 Publication1
Mutagenesisi241E → A: Loss of deoxyhypusine monooxygenase activity. Loss of iron-binding. 1 Publication1

Organism-specific databases

DisGeNETi83475
OpenTargetsiENSG00000129932
PharmGKBiPA142671678

Polymorphism and mutation databases

BioMutaiDOHH
DMDMi74733193

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002485751 – 302Deoxyhypusine hydroxylaseAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9BU89
MaxQBiQ9BU89
PaxDbiQ9BU89
PeptideAtlasiQ9BU89
PRIDEiQ9BU89
ProteomicsDBi79065

PTM databases

iPTMnetiQ9BU89
PhosphoSitePlusiQ9BU89

Expressioni

Gene expression databases

BgeeiENSG00000129932 Expressed in 239 organ(s), highest expression level in globus pallidus
CleanExiHS_DOHH
GenevisibleiQ9BU89 HS

Organism-specific databases

HPAiHPA041953

Interactioni

Protein-protein interaction databases

BioGridi123662, 14 interactors
IntActiQ9BU89, 1 interactor
STRINGi9606.ENSP00000250937

Structurei

Secondary structure

1302
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BU89
SMRiQ9BU89
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati54 – 80HEAT-like PBS-type 1Add BLAST27
Repeati87 – 113HEAT-like PBS-type 2Add BLAST27
Repeati174 – 200HEAT-like PBS-type 3Add BLAST27
Repeati205 – 231HEAT-like PBS-type 4Add BLAST27
Repeati238 – 264HEAT-like PBS-type 5Add BLAST27

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0567 Eukaryota
COG1413 LUCA
GeneTreeiENSGT00500000044957
HOGENOMiHOG000248665
HOVERGENiHBG081460
InParanoidiQ9BU89
KOiK06072
OMAiPEFQYAD
OrthoDBiEOG091G0E2R
PhylomeDBiQ9BU89
TreeFamiTF105626

Family and domain databases

Gene3Di1.25.10.10, 2 hits
HAMAPiMF_03101 Deoxyhypusine_hydroxylase, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR027517 Deoxyhypusine_hydroxylase
IPR021133 HEAT_type_2
IPR004155 PBS_lyase_HEAT
SMARTiView protein in SMART
SM00567 EZ_HEAT, 6 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9BU89-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVTEQEVDAI GQTLVDPKQP LQARFRALFT LRGLGGPGAI AWISQAFDDD
60 70 80 90 100
SALLKHELAY CLGQMQDARA IPMLVDVLQD TRQEPMVRHE AGEALGAIGD
110 120 130 140 150
PEVLEILKQY SSDPVIEVAE TCQLAVRRLE WLQQHGGEPA AGPYLSVDPA
160 170 180 190 200
PPAEERDVGR LREALLDESR PLFERYRAMF ALRNAGGEEA ALALAEGLHC
210 220 230 240 250
GSALFRHEVG YVLGQLQHEA AVPQLAAALA RCTENPMVRH ECAEALGAIA
260 270 280 290 300
RPACLAALQA HADDPERVVR ESCEVALDMY EHETGRAFQY ADGLEQLRGA

PS
Length:302
Mass (Da):32,904
Last modified:June 1, 2001 - v1
Checksum:iCDA86E5549B98A2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005551 Genomic DNA Translation: AAC33193.1
AC093052 Genomic DNA No translation available.
BC002817 mRNA Translation: AAH02817.1
BC009863 mRNA Translation: AAH09863.1
CCDSiCCDS12108.1
RefSeqiNP_001138637.1, NM_001145165.1
NP_112594.1, NM_031304.4
UniGeneiHs.515064
Hs.609012

Genome annotation databases

EnsembliENST00000427575; ENSP00000398882; ENSG00000129932
GeneIDi83475
KEGGihsa:83475
UCSCiuc002lxs.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005551 Genomic DNA Translation: AAC33193.1
AC093052 Genomic DNA No translation available.
BC002817 mRNA Translation: AAH02817.1
BC009863 mRNA Translation: AAH09863.1
CCDSiCCDS12108.1
RefSeqiNP_001138637.1, NM_001145165.1
NP_112594.1, NM_031304.4
UniGeneiHs.515064
Hs.609012

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4D4ZX-ray1.70A/B1-289[»]
4D50X-ray1.70A/B1-289[»]
ProteinModelPortaliQ9BU89
SMRiQ9BU89
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123662, 14 interactors
IntActiQ9BU89, 1 interactor
STRINGi9606.ENSP00000250937

PTM databases

iPTMnetiQ9BU89
PhosphoSitePlusiQ9BU89

Polymorphism and mutation databases

BioMutaiDOHH
DMDMi74733193

Proteomic databases

EPDiQ9BU89
MaxQBiQ9BU89
PaxDbiQ9BU89
PeptideAtlasiQ9BU89
PRIDEiQ9BU89
ProteomicsDBi79065

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000427575; ENSP00000398882; ENSG00000129932
GeneIDi83475
KEGGihsa:83475
UCSCiuc002lxs.4 human

Organism-specific databases

CTDi83475
DisGeNETi83475
EuPathDBiHostDB:ENSG00000129932.7
GeneCardsiDOHH
HGNCiHGNC:28662 DOHH
HPAiHPA041953
MIMi611262 gene
neXtProtiNX_Q9BU89
OpenTargetsiENSG00000129932
PharmGKBiPA142671678
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0567 Eukaryota
COG1413 LUCA
GeneTreeiENSGT00500000044957
HOGENOMiHOG000248665
HOVERGENiHBG081460
InParanoidiQ9BU89
KOiK06072
OMAiPEFQYAD
OrthoDBiEOG091G0E2R
PhylomeDBiQ9BU89
TreeFamiTF105626

Enzyme and pathway databases

UniPathwayi
UPA00354

BioCyciMetaCyc:HS05318-MONOMER
BRENDAi1.14.99.29 2681
ReactomeiR-HSA-204626 Hypusine synthesis from eIF5A-lysine

Miscellaneous databases

GenomeRNAii83475
PROiPR:Q9BU89
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000129932 Expressed in 239 organ(s), highest expression level in globus pallidus
CleanExiHS_DOHH
GenevisibleiQ9BU89 HS

Family and domain databases

Gene3Di1.25.10.10, 2 hits
HAMAPiMF_03101 Deoxyhypusine_hydroxylase, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR027517 Deoxyhypusine_hydroxylase
IPR021133 HEAT_type_2
IPR004155 PBS_lyase_HEAT
SMARTiView protein in SMART
SM00567 EZ_HEAT, 6 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50077 HEAT_REPEAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDOHH_HUMAN
AccessioniPrimary (citable) accession number: Q9BU89
Secondary accession number(s): O75265
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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