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Protein

Acidic leucine-rich nuclear phosphoprotein 32 family member E

Gene

ANP32E

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AFZ from the nucleosome: removes H2A.Z/H2AFZ from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AFZ in the nucleosome. May stabilize the evicted H2A.Z/H2AFZ-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.1 Publication

GO - Molecular functioni

  • histone binding Source: UniProtKB
  • phosphatase inhibitor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChaperone, Chromatin regulator

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic leucine-rich nuclear phosphoprotein 32 family member E
Alternative name(s):
LANP-like protein
Short name:
LANP-L
Gene namesi
Name:ANP32E
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143401.14
HGNCiHGNC:16673 ANP32E
MIMi609611 gene
neXtProtiNX_Q9BTT0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi218 – 222LSYLM → ASYAA in ANP32E-m1; abolished ability to interact with the H2A.Z/H2AFZ-H2B dimer. 1 Publication5
Mutagenesisi232 – 235DDDY → ADAA in ANP32E-m2; abolished ability to interact with the H2A.Z/H2AFZ-H2B dimer. 1 Publication4

Organism-specific databases

OpenTargetsiENSG00000143401
PharmGKBiPA134880751

Polymorphism and mutation databases

DMDMi30580363

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001375991 – 268Acidic leucine-rich nuclear phosphoprotein 32 family member EAdd BLAST268

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9BTT0
MaxQBiQ9BTT0
PaxDbiQ9BTT0
PeptideAtlasiQ9BTT0
PRIDEiQ9BTT0
ProteomicsDBi79007
TopDownProteomicsiQ9BTT0-1 [Q9BTT0-1]

PTM databases

iPTMnetiQ9BTT0
PhosphoSitePlusiQ9BTT0

Expressioni

Tissue specificityi

Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney.1 Publication

Gene expression databases

BgeeiENSG00000143401 Expressed in 238 organ(s), highest expression level in bone marrow
CleanExiHS_ANP32E
ExpressionAtlasiQ9BTT0 baseline and differential
GenevisibleiQ9BTT0 HS

Organism-specific databases

HPAiCAB021114
HPA063133
HPA067561

Interactioni

Subunit structurei

Interacts with the importin alpha KPNA1 and KPNA2 (By similarity). Component of a SWR1-like complex, composed of EP400, KAT5/TIP60, TRRAP, BRD8, RUVBL1, RUVBL2, ING3 and ANP32E; the complex does not contain SRCAP. Interacts with H2A.Z/H2AFZ.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi123548, 37 interactors
IntActiQ9BTT0, 10 interactors
STRINGi9606.ENSP00000324074

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BTT0
SMRiQ9BTT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 38LRR 1Add BLAST21
Repeati43 – 64LRR 2Add BLAST22
Repeati65 – 87LRR 3Add BLAST23
Repeati89 – 110LRR 4Add BLAST22
Domaini123 – 161LRRCTAdd BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 268ZID domainAdd BLAST54

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi151 – 266Asp/Glu-rich (highly acidic)Add BLAST116

Domaini

The H2A.Z-interacting domain (ZID) mediates a direct interaction with H2A.Z/H2AFZ.1 Publication

Sequence similaritiesi

Belongs to the ANP32 family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG2739 Eukaryota
ENOG4111HZT LUCA
GeneTreeiENSGT00560000077130
HOGENOMiHOG000007361
HOVERGENiHBG053102
InParanoidiQ9BTT0
KOiK18648
OMAiRCPNLTY
OrthoDBiEOG091G0R8Z
PhylomeDBiQ9BTT0
TreeFamiTF317206

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
PROSITEiView protein in PROSITE
PS51450 LRR, 4 hits

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BTT0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEMKKKINLE LRNRSPEEVT ELVLDNCLCV NGEIEGLNDT FKELEFLSMA
60 70 80 90 100
NVELSSLARL PSLNKLRKLE LSDNIISGGL EVLAEKCPNL TYLNLSGNKI
110 120 130 140 150
KDLSTVEALQ NLKNLKSLDL FNCEITNLED YRESIFELLQ QITYLDGFDQ
160 170 180 190 200
EDNEAPDSEE EDDEDGDEDD EEEEENEAGP PEGYEEEEEE EEEEDEDEDE
210 220 230 240 250
DEDEAGSELG EGEEEVGLSY LMKEEIQDEE DDDDYVEEGE EEEEEEEGGL
260
RGEKRKRDAE DDGEEEDD
Length:268
Mass (Da):30,692
Last modified:June 1, 2001 - v1
Checksum:i99D74AF4B59BF971
GO
Isoform 2 (identifier: Q9BTT0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-109: Missing.

Note: No experimental confirmation available.
Show »
Length:227
Mass (Da):26,321
Checksum:i059CB2D637B6C426
GO
Isoform 3 (identifier: Q9BTT0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Note: Gene prediction based on EST data.
Show »
Length:220
Mass (Da):25,125
Checksum:iC7564983F775F210
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5TB25Q5TB25_HUMAN
Acidic (Leucine-rich) nuclear phosp...
ANP32E RP4-790G17.1-003
136Annotation score:
E9PI45E9PI45_HUMAN
Acidic leucine-rich nuclear phospho...
ANP32E
105Annotation score:
E9PLC4E9PLC4_HUMAN
Acidic leucine-rich nuclear phospho...
ANP32E
165Annotation score:
Q5TB19Q5TB19_HUMAN
Acidic (Leucine-rich) nuclear phosp...
ANP32E RP4-790G17.1-004
149Annotation score:
E9PPH5E9PPH5_HUMAN
Acidic leucine-rich nuclear phospho...
ANP32E
114Annotation score:
H0YCE2H0YCE2_HUMAN
Acidic leucine-rich nuclear phospho...
ANP32E
100Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185 – 186Missing in AAL25814 (PubMed:12438741).Curated2
Sequence conflicti185 – 186Missing in BAC04505 (PubMed:14702039).Curated2
Sequence conflicti185 – 186Missing in BAG64448 (PubMed:14702039).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0472621 – 48Missing in isoform 3. CuratedAdd BLAST48
Alternative sequenceiVSP_04561869 – 109Missing in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057381 mRNA Translation: AAL25814.1
AK095228 mRNA Translation: BAC04505.1
AK092672 mRNA Translation: BAC03942.1
AK303392 mRNA Translation: BAG64448.1
AL832674 mRNA No translation available.
AL138795 Genomic DNA Translation: CAI22806.1
AL138795 Genomic DNA Translation: CAI22808.1
CH471121 Genomic DNA Translation: EAW53575.1
CH471121 Genomic DNA Translation: EAW53577.1
BC003380 mRNA Translation: AAH03380.1
CCDSiCCDS44214.1 [Q9BTT0-2]
CCDS44215.1 [Q9BTT0-3]
CCDS946.1 [Q9BTT0-1]
RefSeqiNP_001129950.1, NM_001136478.3 [Q9BTT0-2]
NP_001129951.1, NM_001136479.2 [Q9BTT0-3]
NP_001267488.1, NM_001280559.1
NP_001267489.1, NM_001280560.1
NP_112182.1, NM_030920.4 [Q9BTT0-1]
UniGeneiHs.656466

Genome annotation databases

EnsembliENST00000436748; ENSP00000393718; ENSG00000143401 [Q9BTT0-2]
ENST00000583931; ENSP00000463154; ENSG00000143401 [Q9BTT0-1]
ENST00000616917; ENSP00000481415; ENSG00000143401 [Q9BTT0-3]
GeneIDi81611
KEGGihsa:81611
UCSCiuc031uxy.2 human [Q9BTT0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057381 mRNA Translation: AAL25814.1
AK095228 mRNA Translation: BAC04505.1
AK092672 mRNA Translation: BAC03942.1
AK303392 mRNA Translation: BAG64448.1
AL832674 mRNA No translation available.
AL138795 Genomic DNA Translation: CAI22806.1
AL138795 Genomic DNA Translation: CAI22808.1
CH471121 Genomic DNA Translation: EAW53575.1
CH471121 Genomic DNA Translation: EAW53577.1
BC003380 mRNA Translation: AAH03380.1
CCDSiCCDS44214.1 [Q9BTT0-2]
CCDS44215.1 [Q9BTT0-3]
CCDS946.1 [Q9BTT0-1]
RefSeqiNP_001129950.1, NM_001136478.3 [Q9BTT0-2]
NP_001129951.1, NM_001136479.2 [Q9BTT0-3]
NP_001267488.1, NM_001280559.1
NP_001267489.1, NM_001280560.1
NP_112182.1, NM_030920.4 [Q9BTT0-1]
UniGeneiHs.656466

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CAYX-ray1.48C215-240[»]
4NFTX-ray2.61E/F185-232[»]
ProteinModelPortaliQ9BTT0
SMRiQ9BTT0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123548, 37 interactors
IntActiQ9BTT0, 10 interactors
STRINGi9606.ENSP00000324074

PTM databases

iPTMnetiQ9BTT0
PhosphoSitePlusiQ9BTT0

Polymorphism and mutation databases

DMDMi30580363

Proteomic databases

EPDiQ9BTT0
MaxQBiQ9BTT0
PaxDbiQ9BTT0
PeptideAtlasiQ9BTT0
PRIDEiQ9BTT0
ProteomicsDBi79007
TopDownProteomicsiQ9BTT0-1 [Q9BTT0-1]

Protocols and materials databases

DNASUi81611
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000436748; ENSP00000393718; ENSG00000143401 [Q9BTT0-2]
ENST00000583931; ENSP00000463154; ENSG00000143401 [Q9BTT0-1]
ENST00000616917; ENSP00000481415; ENSG00000143401 [Q9BTT0-3]
GeneIDi81611
KEGGihsa:81611
UCSCiuc031uxy.2 human [Q9BTT0-1]

Organism-specific databases

CTDi81611
EuPathDBiHostDB:ENSG00000143401.14
GeneCardsiANP32E
HGNCiHGNC:16673 ANP32E
HPAiCAB021114
HPA063133
HPA067561
MIMi609611 gene
neXtProtiNX_Q9BTT0
OpenTargetsiENSG00000143401
PharmGKBiPA134880751
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2739 Eukaryota
ENOG4111HZT LUCA
GeneTreeiENSGT00560000077130
HOGENOMiHOG000007361
HOVERGENiHBG053102
InParanoidiQ9BTT0
KOiK18648
OMAiRCPNLTY
OrthoDBiEOG091G0R8Z
PhylomeDBiQ9BTT0
TreeFamiTF317206

Miscellaneous databases

ChiTaRSiANP32E human
GeneWikiiANP32E
GenomeRNAii81611
PROiPR:Q9BTT0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143401 Expressed in 238 organ(s), highest expression level in bone marrow
CleanExiHS_ANP32E
ExpressionAtlasiQ9BTT0 baseline and differential
GenevisibleiQ9BTT0 HS

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
PROSITEiView protein in PROSITE
PS51450 LRR, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiAN32E_HUMAN
AccessioniPrimary (citable) accession number: Q9BTT0
Secondary accession number(s): B4E0I6
, E9PEA6, Q5TB18, Q5TB20, Q8N1S4, Q8WWW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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