UniProtKB - Q9BTM9 (URM1_HUMAN)
Protein
Ubiquitin-related modifier 1
Gene
URM1
Organism
Homo sapiens (Human)
Status
Functioni
Acts as a sulfur carrier required for 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm5S2U. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.UniRule annotation2 Publications
: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis Pathwayi
This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationView all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.
GO - Molecular functioni
- protein tag Source: GO_Central
- sulfur carrier activity Source: UniProtKB
GO - Biological processi
- protein urmylation Source: GO_Central
- tRNA modification Source: Reactome
- tRNA thio-modification Source: UniProtKB
- tRNA wobble uridine modification Source: UniProtKB
Keywordsi
Biological process | tRNA processing, Ubl conjugation pathway |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000167118-MONOMER |
PathwayCommonsi | Q9BTM9 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
UniPathwayi | UPA00988 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-related modifier 1UniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28378, URM1 |
MIMi | 612693, gene |
neXtProti | NX_Q9BTM9 |
VEuPathDBi | HostDB:ENSG00000167118.10 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 81605 |
OpenTargetsi | ENSG00000167118 |
PharmGKBi | PA162408677 |
Miscellaneous databases
Pharosi | Q9BTM9, Tbio |
Genetic variation databases
BioMutai | URM1 |
DMDMi | 68565265 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089714 | 1 – 101 | Ubiquitin-related modifier 1Add BLAST | 101 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 101 | 1-thioglycineUniRule annotation1 Publication | 1 | |
Cross-linki | 101 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)UniRule annotation |
Post-translational modificationi
C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.UniRule annotation1 Publication
Keywords - PTMi
Isopeptide bondProteomic databases
EPDi | Q9BTM9 |
jPOSTi | Q9BTM9 |
MassIVEi | Q9BTM9 |
MaxQBi | Q9BTM9 |
PaxDbi | Q9BTM9 |
PeptideAtlasi | Q9BTM9 |
PRIDEi | Q9BTM9 |
ProteomicsDBi | 78999 [Q9BTM9-1] 79000 [Q9BTM9-2] |
TopDownProteomicsi | Q9BTM9-1 [Q9BTM9-1] |
PTM databases
iPTMneti | Q9BTM9 |
PhosphoSitePlusi | Q9BTM9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000167118, Expressed in endometrium epithelium and 230 other tissues |
ExpressionAtlasi | Q9BTM9, baseline and differential |
Genevisiblei | Q9BTM9, HS |
Organism-specific databases
HPAi | ENSG00000167118, Low tissue specificity |
Interactioni
Subunit structurei
Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.
UniRule annotation1 PublicationBinary interactionsi
Hide detailsQ9BTM9
With | #Exp. | IntAct |
---|---|---|
CSE1L [P55060] | 2 | EBI-714589,EBI-286709 |
CTU1 [Q7Z7A3] | 7 | EBI-714589,EBI-15741880 |
MOCS3 [O95396] | 4 | EBI-714589,EBI-373206 |
USP15 [Q9Y4E8] | 2 | EBI-714589,EBI-1043104 |
Protein-protein interaction databases
BioGRIDi | 123542, 20 interactors |
DIPi | DIP-48631N |
IntActi | Q9BTM9, 27 interactors |
STRINGi | 9606.ENSP00000412922 |
Miscellaneous databases
RNActi | Q9BTM9, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the URM1 family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG4146, Eukaryota |
GeneTreei | ENSGT00390000005101 |
HOGENOMi | CLU_148208_0_1_1 |
InParanoidi | Q9BTM9 |
OMAi | EMLFSDQ |
OrthoDBi | 1389193at2759 |
PhylomeDBi | Q9BTM9 |
TreeFami | TF336363 |
Family and domain databases
CDDi | cd01764, Ubl_Urm1, 1 hit |
Gene3Di | 3.10.20.30, 1 hit |
HAMAPi | MF_03048, Urm1, 1 hit |
InterProi | View protein in InterPro IPR012675, Beta-grasp_dom_sf IPR016155, Mopterin_synth/thiamin_S_b IPR015221, Urm1 |
PANTHERi | PTHR14986, PTHR14986, 1 hit |
Pfami | View protein in Pfam PF09138, Urm1, 1 hit |
PIRSFi | PIRSF037379, Ubiquitin-related_modifier_1, 1 hit |
SUPFAMi | SSF54285, SSF54285, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9BTM9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAPLSVEVE FGGGAELLFD GIKKHRVTLP GQEEPWDIRN LLIWIKKNLL
60 70 80 90 100
KERPELFIQG DSVRPGILVL INDADWELLG ELDYQLQDQD SVLFISTLHG
G
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5T4A9 | Q5T4A9_HUMAN | Ubiquitin-related modifier 1 | URM1 | 63 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054296 | 64 – 101 | Missing in isoform 3. CuratedAdd BLAST | 38 | |
Alternative sequenceiVSP_040026 | 79 – 101 | LGELD…TLHGG → LVSTLGDIPPPAPALAASVG KRWASPQAHIEWLGNPPPHS SPTLRLLESPTPGEEGMGSW GHGSTPPS in isoform 2. 1 PublicationAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK001880 mRNA Translation: BAG50985.1 AK300877 mRNA Translation: BAG62520.1 AL359091 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW87782.1 BC003581 mRNA Translation: AAH03581.1 |
CCDSi | CCDS48035.1 [Q9BTM9-2] CCDS59148.1 [Q9BTM9-3] CCDS6900.1 [Q9BTM9-1] |
RefSeqi | NP_001129419.1, NM_001135947.2 [Q9BTM9-2] NP_001252511.1, NM_001265582.1 [Q9BTM9-3] NP_112176.1, NM_030914.3 [Q9BTM9-1] |
Genome annotation databases
Ensembli | ENST00000372850; ENSP00000361941; ENSG00000167118 [Q9BTM9-3] ENST00000372853; ENSP00000361944; ENSG00000167118 [Q9BTM9-1] ENST00000483206; ENSP00000501135; ENSG00000167118 [Q9BTM9-2] |
GeneIDi | 81605 |
KEGGi | hsa:81605 |
UCSCi | uc004buv.3, human [Q9BTM9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK001880 mRNA Translation: BAG50985.1 AK300877 mRNA Translation: BAG62520.1 AL359091 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW87782.1 BC003581 mRNA Translation: AAH03581.1 |
CCDSi | CCDS48035.1 [Q9BTM9-2] CCDS59148.1 [Q9BTM9-3] CCDS6900.1 [Q9BTM9-1] |
RefSeqi | NP_001129419.1, NM_001135947.2 [Q9BTM9-2] NP_001252511.1, NM_001265582.1 [Q9BTM9-3] NP_112176.1, NM_030914.3 [Q9BTM9-1] |
3D structure databases
SMRi | Q9BTM9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 123542, 20 interactors |
DIPi | DIP-48631N |
IntActi | Q9BTM9, 27 interactors |
STRINGi | 9606.ENSP00000412922 |
PTM databases
iPTMneti | Q9BTM9 |
PhosphoSitePlusi | Q9BTM9 |
Genetic variation databases
BioMutai | URM1 |
DMDMi | 68565265 |
Proteomic databases
EPDi | Q9BTM9 |
jPOSTi | Q9BTM9 |
MassIVEi | Q9BTM9 |
MaxQBi | Q9BTM9 |
PaxDbi | Q9BTM9 |
PeptideAtlasi | Q9BTM9 |
PRIDEi | Q9BTM9 |
ProteomicsDBi | 78999 [Q9BTM9-1] 79000 [Q9BTM9-2] |
TopDownProteomicsi | Q9BTM9-1 [Q9BTM9-1] |
Protocols and materials databases
Antibodypediai | 17464, 128 antibodies |
Genome annotation databases
Ensembli | ENST00000372850; ENSP00000361941; ENSG00000167118 [Q9BTM9-3] ENST00000372853; ENSP00000361944; ENSG00000167118 [Q9BTM9-1] ENST00000483206; ENSP00000501135; ENSG00000167118 [Q9BTM9-2] |
GeneIDi | 81605 |
KEGGi | hsa:81605 |
UCSCi | uc004buv.3, human [Q9BTM9-1] |
Organism-specific databases
CTDi | 81605 |
DisGeNETi | 81605 |
GeneCardsi | URM1 |
HGNCi | HGNC:28378, URM1 |
HPAi | ENSG00000167118, Low tissue specificity |
MIMi | 612693, gene |
neXtProti | NX_Q9BTM9 |
OpenTargetsi | ENSG00000167118 |
PharmGKBi | PA162408677 |
VEuPathDBi | HostDB:ENSG00000167118.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4146, Eukaryota |
GeneTreei | ENSGT00390000005101 |
HOGENOMi | CLU_148208_0_1_1 |
InParanoidi | Q9BTM9 |
OMAi | EMLFSDQ |
OrthoDBi | 1389193at2759 |
PhylomeDBi | Q9BTM9 |
TreeFami | TF336363 |
Enzyme and pathway databases
UniPathwayi | UPA00988 |
BioCyci | MetaCyc:ENSG00000167118-MONOMER |
PathwayCommonsi | Q9BTM9 |
Reactomei | R-HSA-6782315, tRNA modification in the nucleus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 81605, 491 hits in 1009 CRISPR screens |
ChiTaRSi | URM1, human |
GenomeRNAii | 81605 |
Pharosi | Q9BTM9, Tbio |
PROi | PR:Q9BTM9 |
RNActi | Q9BTM9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167118, Expressed in endometrium epithelium and 230 other tissues |
ExpressionAtlasi | Q9BTM9, baseline and differential |
Genevisiblei | Q9BTM9, HS |
Family and domain databases
CDDi | cd01764, Ubl_Urm1, 1 hit |
Gene3Di | 3.10.20.30, 1 hit |
HAMAPi | MF_03048, Urm1, 1 hit |
InterProi | View protein in InterPro IPR012675, Beta-grasp_dom_sf IPR016155, Mopterin_synth/thiamin_S_b IPR015221, Urm1 |
PANTHERi | PTHR14986, PTHR14986, 1 hit |
Pfami | View protein in Pfam PF09138, Urm1, 1 hit |
PIRSFi | PIRSF037379, Ubiquitin-related_modifier_1, 1 hit |
SUPFAMi | SSF54285, SSF54285, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | URM1_HUMAN | |
Accessioni | Q9BTM9Primary (citable) accession number: Q9BTM9 Secondary accession number(s): B3KMH3, B4DV08, Q5T4B4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families