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Protein

Trichoplein keratin filament-binding protein

Gene

TCHP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells (PubMed:15731013, PubMed:18931701). Is a negative regulator of ciliogenesis (PubMed:25270598).3 Publications

GO - Biological processi

  • apoptotic process Source: UniProtKB
  • cell projection organization Source: UniProtKB-KW
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of cilium assembly Source: UniProtKB

Keywordsi

Biological processApoptosis, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Trichoplein keratin filament-binding protein
Short name:
Protein TCHP
Alternative name(s):
Mitochondrial protein with oncostatic activity
Short name:
Mitostatin
Tumor suppressor protein
Gene namesi
Name:TCHPImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000139437.17
HGNCiHGNC:28135 TCHP
MIMi612654 gene
neXtProtiNX_Q9BT92

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50K → R: Decreased ubiquitination. Negative effect on ubiquitination is higher when associated with R-57. 1 Publication1
Mutagenesisi57K → R: Decreased ubiquitination. Negative effect on ubiquitination is higher when associated with R-50. 1 Publication1

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi84260
OpenTargetsiENSG00000139437
PharmGKBiPA143485629

Polymorphism and mutation databases

BioMutaiTCHP
DMDMi74733103

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002926091 – 498Trichoplein keratin filament-binding proteinAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki57Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Ubiquitinated. Ubiquitination by the BCR(KCTD17) E3 ubiquitin ligase complex results in proteasomal degradation, and induces ciliogenesis.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9BT92
MaxQBiQ9BT92
PaxDbiQ9BT92
PeptideAtlasiQ9BT92
PRIDEiQ9BT92
ProteomicsDBi78961

PTM databases

iPTMnetiQ9BT92
PhosphoSitePlusiQ9BT92

Expressioni

Tissue specificityi

Expressed at high levels in normal urothelial and breast epithelial cells. Also expressed in the smooth muscle and endothelial cells. Reduced expression seen in advanced bladder and breast carcinomas (at protein level). Ubiquitous. Expressed at highest levels in the heart, skeletal muscle, kidney, liver and testis.1 Publication

Gene expression databases

BgeeiENSG00000139437 Expressed in 185 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_TCHP
ExpressionAtlasiQ9BT92 baseline and differential
GenevisibleiQ9BT92 HS

Organism-specific databases

HPAiHPA038638
HPA061543

Interactioni

Subunit structurei

Interacts specifically with keratin proteins including, KRT5, KRT6A, KRT8, KRT14, KRT16 and KRT18 (PubMed:15731013). Interacts with KCTD17 (PubMed:25270598).2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi123987, 52 interactors
IntActiQ9BT92, 76 interactors
MINTiQ9BT92
STRINGi9606.ENSP00000324404

Structurei

3D structure databases

ProteinModelPortaliQ9BT92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 498Interaction with keratin proteins1 PublicationAdd BLAST426
Regioni259 – 425Trichohyalin/plectin homology domain1 PublicationAdd BLAST167

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili11 – 39Sequence analysisAdd BLAST29
Coiled coili66 – 136Sequence analysisAdd BLAST71
Coiled coili163 – 353Sequence analysisAdd BLAST191
Coiled coili380 – 479Sequence analysisAdd BLAST100

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi70 – 482Glu-richSequence analysisAdd BLAST413

Sequence similaritiesi

Belongs to the TCHP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IIAX Eukaryota
ENOG410ZEPV LUCA
GeneTreeiENSGT00730000111170
HOGENOMiHOG000006708
HOVERGENiHBG097262
InParanoidiQ9BT92
KOiK16811
OMAiPKLREME
OrthoDBiEOG091G0FWB
PhylomeDBiQ9BT92
TreeFamiTF329032

Family and domain databases

InterProiView protein in InterPro
IPR026773 TCHP
PANTHERiPTHR31183:SF2 PTHR31183:SF2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9BT92-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALPTLPSYW CSQQRLNQQL ARQREQEARL RQQWEQNSRY FRMSDICSSK
60 70 80 90 100
QAEWSSKTSY QRSMHAYQRE KMKEEKRRSL EARREKLRQL MQEEQDLLAR
110 120 130 140 150
ELEELRLSMN LQERRIREQH GKLKSAKEEQ RKLIAEQLLY EHWKKNNPKL
160 170 180 190 200
REMELDLHQK HVVNSWEMQK EEKKQQEATA EQENKRYENE YERARREALE
210 220 230 240 250
RMKAEEERRQ LEDKLQAEAL LQQMEELKLK EVEATKLKKE QENLLKQRWE
260 270 280 290 300
LERLEEERKQ MEAFRQKAEL GRFLRHQYNA QLSRRTQQIQ EELEADRRIL
310 320 330 340 350
QALLEKEDES QRLHLARREQ VMADVAWMKQ AIEEQLQLER AREAELQMLL
360 370 380 390 400
REEAKEMWEK REAEWARERS ARDRLMSEVL TGRQQQIQEK IEQNRRAQEE
410 420 430 440 450
SLKHREQLIR NLEEVRELAR REKEESEKLK SARKQELEAQ VAERRLQAWE
460 470 480 490
ADQQEEEEEE EARRVEQLSD ALLQQEAETM AEQGYRPKPY GHPKIAWN
Length:498
Mass (Da):61,072
Last modified:June 1, 2001 - v1
Checksum:i7D1E8A66C56516F8
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5GWH6F5GWH6_HUMAN
Trichoplein keratin filament-bindin...
TCHP
174Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti326A → V in BAC03960 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06405644S → P in a gastric carcinoma sample. 1 PublicationCorresponds to variant dbSNP:rs202208566Ensembl.1
Natural variantiVAR_06405793E → K in a pancreatic carcinoma sample. 1 PublicationCorresponds to variant dbSNP:rs200027650Ensembl.1
Natural variantiVAR_053924127K → R. Corresponds to variant dbSNP:rs10774978Ensembl.1
Natural variantiVAR_053925417E → K. Corresponds to variant dbSNP:rs16940680Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007230 mRNA Translation: AAG12971.1
AK092736 mRNA Translation: BAC03960.1
BC004285 mRNA Translation: AAH04285.1
CCDSiCCDS9137.1
RefSeqiNP_001137324.1, NM_001143852.1
NP_115676.1, NM_032300.4
XP_011537138.1, XM_011538836.2
XP_011537139.1, XM_011538837.2
UniGeneiHs.410924

Genome annotation databases

EnsembliENST00000312777; ENSP00000324404; ENSG00000139437
ENST00000405876; ENSP00000384520; ENSG00000139437
ENST00000544838; ENSP00000440838; ENSG00000139437
GeneIDi84260
KEGGihsa:84260
UCSCiuc001tpn.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007230 mRNA Translation: AAG12971.1
AK092736 mRNA Translation: BAC03960.1
BC004285 mRNA Translation: AAH04285.1
CCDSiCCDS9137.1
RefSeqiNP_001137324.1, NM_001143852.1
NP_115676.1, NM_032300.4
XP_011537138.1, XM_011538836.2
XP_011537139.1, XM_011538837.2
UniGeneiHs.410924

3D structure databases

ProteinModelPortaliQ9BT92
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123987, 52 interactors
IntActiQ9BT92, 76 interactors
MINTiQ9BT92
STRINGi9606.ENSP00000324404

PTM databases

iPTMnetiQ9BT92
PhosphoSitePlusiQ9BT92

Polymorphism and mutation databases

BioMutaiTCHP
DMDMi74733103

Proteomic databases

EPDiQ9BT92
MaxQBiQ9BT92
PaxDbiQ9BT92
PeptideAtlasiQ9BT92
PRIDEiQ9BT92
ProteomicsDBi78961

Protocols and materials databases

DNASUi84260
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312777; ENSP00000324404; ENSG00000139437
ENST00000405876; ENSP00000384520; ENSG00000139437
ENST00000544838; ENSP00000440838; ENSG00000139437
GeneIDi84260
KEGGihsa:84260
UCSCiuc001tpn.4 human

Organism-specific databases

CTDi84260
DisGeNETi84260
EuPathDBiHostDB:ENSG00000139437.17
GeneCardsiTCHP
HGNCiHGNC:28135 TCHP
HPAiHPA038638
HPA061543
MIMi612654 gene
neXtProtiNX_Q9BT92
OpenTargetsiENSG00000139437
PharmGKBiPA143485629
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIAX Eukaryota
ENOG410ZEPV LUCA
GeneTreeiENSGT00730000111170
HOGENOMiHOG000006708
HOVERGENiHBG097262
InParanoidiQ9BT92
KOiK16811
OMAiPKLREME
OrthoDBiEOG091G0FWB
PhylomeDBiQ9BT92
TreeFamiTF329032

Miscellaneous databases

ChiTaRSiTCHP human
GeneWikiiTCHP
GenomeRNAii84260
PROiPR:Q9BT92
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000139437 Expressed in 185 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_TCHP
ExpressionAtlasiQ9BT92 baseline and differential
GenevisibleiQ9BT92 HS

Family and domain databases

InterProiView protein in InterPro
IPR026773 TCHP
PANTHERiPTHR31183:SF2 PTHR31183:SF2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTCHP_HUMAN
AccessioniPrimary (citable) accession number: Q9BT92
Secondary accession number(s): Q8NAG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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