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Protein

Polycomb group RING finger protein 1

Gene

PCGF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6; this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down-regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:26151332). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed:26151332). Regulates the expression of DPPA4 and NANOG in the NT2 embryonic carcinoma cells (PubMed:26687479).5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri47 – 86RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • promoter-specific chromatin binding Source: GO_Central
  • protein C-terminus binding Source: UniProtKB

GO - Biological processi

  • chromatin silencing Source: GO_Central
  • histone H2A-K119 monoubiquitination Source: UniProtKB
  • histone H2A monoubiquitination Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: GO_Central
  • regulation of transcription, DNA-templated Source: UniProtKB

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

SIGNORiQ9BSM1

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb group RING finger protein 1
Alternative name(s):
Nervous system Polycomb-1
Short name:
NSPc1
RING finger protein 68
Gene namesi
Name:PCGF1
Synonyms:NSPC1, RNF68
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115289.12
HGNCiHGNC:17615 PCGF1
MIMi610231 gene
neXtProtiNX_Q9BSM1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109Y → F: Marked decrease of repressor activity. May be a kinase phosphorylation site. 1 Publication1
Mutagenesisi191Y → A: Abolishes interaction with BCOR and BCORL1. 1 Publication1
Mutagenesisi193R → A: Abolishes interaction with BCOR and BCORL1. 1 Publication1
Mutagenesisi195S → F: Abolishes repressor activity. May be a PKC phosphorylation site. 1 Publication1
Mutagenesisi206V → D: Abolishes interaction with BCOR and BCORL1. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000115289
PharmGKBiPA134976631

Polymorphism and mutation databases

BioMutaiPCGF1
DMDMi223590124

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002778552 – 259Polycomb group RING finger protein 1Add BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei3PhosphoserineCombined sources1
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki88Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9BSM1
MaxQBiQ9BSM1
PaxDbiQ9BSM1
PeptideAtlasiQ9BSM1
PRIDEiQ9BSM1
ProteomicsDBi78914
78915 [Q9BSM1-3]

PTM databases

iPTMnetiQ9BSM1
PhosphoSitePlusiQ9BSM1

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000115289 Expressed in 215 organ(s), highest expression level in oocyte
CleanExiHS_PCGF1
GenevisibleiQ9BSM1 HS

Organism-specific databases

HPAiHPA011356
HPA069156

Interactioni

Subunit structurei

Component of the repressive BCOR complex containing a Polycomb group subcomplex at least composed of RYBP, RING1 and RNF2/RING2 (PubMed:16943429). Specifically interacts with BCOR, RING1 and RNF2/RING2 (PubMed:16943429, PubMed:26687479, PubMed:23523425). Also interacts with BCORL1, the interaction is direct (PubMed:23523425). Component of a PRC1-like complex (PubMed:21282530, PubMed:26151332). Interacts with CBX6, CBX7 and CBX8 (PubMed:21282530). Interacts with DPPA4, NANOG, POU5F1 and RYBP (PubMed:26687479).5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124243, 107 interactors
CORUMiQ9BSM1
DIPiDIP-52708N
IntActiQ9BSM1, 42 interactors
STRINGi9606.ENSP00000233630

Structurei

Secondary structure

1259
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BSM1
SMRiQ9BSM1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 247Required for repressor activityAdd BLAST162
Regioni167 – 255Sufficient for interaction with BCOR and BCORL11 PublicationAdd BLAST89

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri47 – 86RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2660 Eukaryota
ENOG410XPCN LUCA
GeneTreeiENSGT00550000074463
HOGENOMiHOG000231946
HOVERGENiHBG052826
InParanoidiQ9BSM1
KOiK11487
OMAiIQPAGDD
OrthoDBiEOG091G0EC9
PhylomeDBiQ9BSM1
TreeFamiTF324206

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039642 E3_Ub_ligase/polycomb_RING
IPR032443 RAWUL
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR10825 PTHR10825, 1 hit
PfamiView protein in Pfam
PF16207 RAWUL, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9BSM1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASPQGGQIA IAMRLRNQLQ SVYKMDPLRN EEEVRVKIKD LNEHIVCCLC
60 70 80 90 100
AGYFVDATTI TECLHTFCKS CIVKYLQTSK YCPMCNIKIH ETQPLLNLKL
110 120 130 140 150
DRVMQDIVYK LVPGLQDSEE KRIREFYQSR GLDRVTQPTG EEPALSNLGL
160 170 180 190 200
PFSSFDHSKA HYYRYDEQLN LCLERLSSGK DKNKSVLQNK YVRCSVRAEV
210 220 230 240 250
RHLRRVLCHR LMLNPQHVQL LFDNEVLPDH MTMKQIWLSR WFGKPSPLLL

QYSVKEKRR
Length:259
Mass (Da):30,346
Last modified:February 10, 2009 - v2
Checksum:i456C9417E53A01B6
GO
Isoform 2 (identifier: Q9BSM1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Show »
Length:176
Mass (Da):20,904
Checksum:i18CE151AA03179E4
GO

Sequence cautioni

The sequence AAH04952 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAP97183 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0363931 – 83Missing in isoform 2. 1 PublicationAdd BLAST83

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087884 mRNA Translation: AAP97183.1 Different initiation.
AC005041 Genomic DNA No translation available.
BC004952 mRNA Translation: AAH04952.1 Different initiation.
CCDSiCCDS1946.2 [Q9BSM1-1]
RefSeqiNP_116062.2, NM_032673.2 [Q9BSM1-1]
UniGeneiHs.316750

Genome annotation databases

EnsembliENST00000233630; ENSP00000233630; ENSG00000115289 [Q9BSM1-1]
GeneIDi84759
KEGGihsa:84759
UCSCiuc002slz.4 human [Q9BSM1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087884 mRNA Translation: AAP97183.1 Different initiation.
AC005041 Genomic DNA No translation available.
BC004952 mRNA Translation: AAH04952.1 Different initiation.
CCDSiCCDS1946.2 [Q9BSM1-1]
RefSeqiNP_116062.2, NM_032673.2 [Q9BSM1-1]
UniGeneiHs.316750

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HPLX-ray2.00B167-255[»]
4HPMX-ray1.85B/D167-255[»]
5JH5X-ray2.55C150-255[»]
ProteinModelPortaliQ9BSM1
SMRiQ9BSM1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124243, 107 interactors
CORUMiQ9BSM1
DIPiDIP-52708N
IntActiQ9BSM1, 42 interactors
STRINGi9606.ENSP00000233630

PTM databases

iPTMnetiQ9BSM1
PhosphoSitePlusiQ9BSM1

Polymorphism and mutation databases

BioMutaiPCGF1
DMDMi223590124

Proteomic databases

EPDiQ9BSM1
MaxQBiQ9BSM1
PaxDbiQ9BSM1
PeptideAtlasiQ9BSM1
PRIDEiQ9BSM1
ProteomicsDBi78914
78915 [Q9BSM1-3]

Protocols and materials databases

DNASUi84759
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000233630; ENSP00000233630; ENSG00000115289 [Q9BSM1-1]
GeneIDi84759
KEGGihsa:84759
UCSCiuc002slz.4 human [Q9BSM1-1]

Organism-specific databases

CTDi84759
EuPathDBiHostDB:ENSG00000115289.12
GeneCardsiPCGF1
HGNCiHGNC:17615 PCGF1
HPAiHPA011356
HPA069156
MIMi610231 gene
neXtProtiNX_Q9BSM1
OpenTargetsiENSG00000115289
PharmGKBiPA134976631
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2660 Eukaryota
ENOG410XPCN LUCA
GeneTreeiENSGT00550000074463
HOGENOMiHOG000231946
HOVERGENiHBG052826
InParanoidiQ9BSM1
KOiK11487
OMAiIQPAGDD
OrthoDBiEOG091G0EC9
PhylomeDBiQ9BSM1
TreeFamiTF324206

Enzyme and pathway databases

SIGNORiQ9BSM1

Miscellaneous databases

ChiTaRSiPCGF1 human
GeneWikiiPCGF1
GenomeRNAii84759
PROiPR:Q9BSM1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115289 Expressed in 215 organ(s), highest expression level in oocyte
CleanExiHS_PCGF1
GenevisibleiQ9BSM1 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR039642 E3_Ub_ligase/polycomb_RING
IPR032443 RAWUL
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR10825 PTHR10825, 1 hit
PfamiView protein in Pfam
PF16207 RAWUL, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCGF1_HUMAN
AccessioniPrimary (citable) accession number: Q9BSM1
Secondary accession number(s): Q7Z506
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 10, 2009
Last modified: November 7, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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