UniProtKB - Q9BSK2 (S2533_HUMAN)
Solute carrier family 25 member 33
SLC25A33
Functioni
Mitochondrial transporter that imports/exports pyrimidine nucleotides into and from mitochondria (PubMed:25320081).
Transports preferentially uracil, thymine, and cytosine (deoxy)nucleoside di- and triphosphates by an antiport mechanism (PubMed:25320081).
Also transports guanine but not adenine (deoxy)nucleotides (PubMed:25320081).
Is inhibited strongly by pyridoxal 5'-phosphate, 4,7-diphenyl-1,10-phenanthroline, tannic acid, and mercurials (mercury dichloride, mersalyl acid, p-hydroxymercuribenzoate) (PubMed:25320081).
Participates in mitochondrial genome maintenance, regulation of mitochondrial membrane potential and mitochondrial respiration (PubMed:20453889).
Upon INS or IGF1 stimulation regulates cell growth and proliferation by controlling mitochondrial DNA replication and transcription, the ratio of mitochondria-to nuclear-encoded components of the electron transport chain resulting in control of mitochondrial ROS production (PubMed:20453889, PubMed:17596519).
Participates in dendritic cell endocytosis and may associate with mitochondrial oxidative phosphorylation (PubMed:14715278).
4 PublicationsKineticsi
- KM=0.16 mM for UTP (at 25 degrees Celsius)1 Publication
- KM=0.18 mM for CTP (at 25 degrees Celsius)1 Publication
- KM=0.08 mM for TTP (at 25 degrees Celsius)1 Publication
- KM=0.30 mM for GTP (at 25 degrees Celsius)1 Publication
- Vmax=98.5 µmol/min/g enzyme toward UTP (at 25 degrees Celsius)1 Publication
- Vmax=22.5 µmol/min/g enzyme toward CTP (at 25 degrees Celsius)1 Publication
- Vmax=50.8 µmol/min/g enzyme toward TTP (at 25 degrees Celsius)1 Publication
- Vmax=15.7 µmol/min/g enzyme toward GTP (at 25 degrees Celsius)1 Publication
GO - Molecular functioni
- pyrimidine nucleotide transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- cellular response to insulin-like growth factor stimulus Source: UniProtKB
- cellular response to insulin stimulus Source: UniProtKB
- mitochondrial genome maintenance Source: UniProtKB
- mitochondrial respiratory chain complex III assembly Source: UniProtKB
- mitochondrial transcription Source: UniProtKB
- mitochondria-nucleus signaling pathway Source: UniProtKB
- mitochondrion organization Source: UniProtKB
- positive regulation of cell growth Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- pyrimidine nucleotide import into mitochondrion Source: UniProtKB
- pyrimidine nucleotide transport Source: UniProtKB
- regulation of mitochondrial membrane potential Source: UniProtKB
- regulation of oxidative phosphorylation Source: UniProtKB
- regulation of reactive oxygen species biosynthetic process Source: UniProtKB
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9BSK2 |
SignaLinki | Q9BSK2 |
Protein family/group databases
TCDBi | 2.A.29.10.7, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 25 member 33Alternative name(s): Bone marrow stromal cell mitochondrial carrier protein Short name: BMSC-MCP Short name: HuBMSC-MCP Protein PNC1 |
Gene namesi | Name:SLC25A33 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29681, SLC25A33 |
MIMi | 610816, gene |
neXtProti | NX_Q9BSK2 |
VEuPathDBi | HostDB:ENSG00000171612 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
- mitochondrial membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 12 – 32 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 49 – 65 | Helical; Name=2Sequence analysisAdd BLAST | 17 | |
Transmembranei | 121 – 141 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 190 – 210 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 233 – 253 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Transmembranei | 298 – 318 | Helical; Name=6Sequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Organism-specific databases
DisGeNETi | 84275 |
OpenTargetsi | ENSG00000171612 |
PharmGKBi | PA162403588 |
Miscellaneous databases
Pharosi | Q9BSK2, Tbio |
Genetic variation databases
BioMutai | SLC25A33 |
DMDMi | 74752304 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000291785 | 1 – 321 | Solute carrier family 25 member 33Add BLAST | 321 |
Proteomic databases
EPDi | Q9BSK2 |
jPOSTi | Q9BSK2 |
MassIVEi | Q9BSK2 |
MaxQBi | Q9BSK2 |
PaxDbi | Q9BSK2 |
PeptideAtlasi | Q9BSK2 |
PRIDEi | Q9BSK2 |
ProteomicsDBi | 78911 |
PTM databases
iPTMneti | Q9BSK2 |
PhosphoSitePlusi | Q9BSK2 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000171612, Expressed in liver and 123 other tissues |
Genevisiblei | Q9BSK2, HS |
Organism-specific databases
HPAi | ENSG00000171612, Tissue enhanced (tongue) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 124002, 43 interactors |
IntActi | Q9BSK2, 5 interactors |
MINTi | Q9BSK2 |
STRINGi | 9606.ENSP00000306328 |
Miscellaneous databases
RNActi | Q9BSK2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 9 – 118 | Solcar 1Add BLAST | 110 | |
Repeati | 126 – 213 | Solcar 2Add BLAST | 88 | |
Repeati | 231 – 315 | Solcar 3Add BLAST | 85 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0757, Eukaryota |
GeneTreei | ENSGT00940000158954 |
HOGENOMi | CLU_015166_6_0_1 |
InParanoidi | Q9BSK2 |
OMAi | PTWAVYM |
OrthoDBi | 1372287at2759 |
PhylomeDBi | Q9BSK2 |
TreeFami | TF314220 |
Family and domain databases
Gene3Di | 1.50.40.10, 2 hits |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MATGGQQKEN TLLHLFAGGC GGTVGAIFTC PLEVIKTRLQ SSRLALRTVY
60 70 80 90 100
YPQVHLGTIS GAGMVRPTSV TPGLFQVLKS ILEKEGPKSL FRGLGPNLVG
110 120 130 140 150
VAPSRAVYFA CYSKAKEQFN GIFVPNSNIV HIFSAGSAAF ITNSLMNPIW
160 170 180 190 200
MVKTRMQLEQ KVRGSKQMNT LQCARYVYQT EGIRGFYRGL TASYAGISET
210 220 230 240 250
IICFAIYESL KKYLKEAPLA SSANGTEKNS TSFFGLMAAA ALSKGCASCI
260 270 280 290 300
AYPHEVIRTR LREEGTKYKS FVQTARLVFR EEGYLAFYRG LFAQLIRQIP
310 320
NTAIVLSTYE LIVYLLEDRT Q
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_032861 | 242 | L → I. Corresponds to variant dbSNP:rs35819756Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF495714 mRNA Translation: AAM18051.1 AJ880283 mRNA Translation: CAI54244.1 AL954705 Genomic DNA No translation available. AL928921 Genomic DNA No translation available. BC004991 mRNA Translation: AAH04991.1 BC073135 mRNA Translation: AAH73135.1 |
CCDSi | CCDS103.1 |
RefSeqi | NP_115691.1, NM_032315.2 |
Genome annotation databases
Ensembli | ENST00000302692.7; ENSP00000306328.5; ENSG00000171612.7 |
GeneIDi | 84275 |
KEGGi | hsa:84275 |
MANE-Selecti | ENST00000302692.7; ENSP00000306328.5; NM_032315.3; NP_115691.1 |
UCSCi | uc001apw.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF495714 mRNA Translation: AAM18051.1 AJ880283 mRNA Translation: CAI54244.1 AL954705 Genomic DNA No translation available. AL928921 Genomic DNA No translation available. BC004991 mRNA Translation: AAH04991.1 BC073135 mRNA Translation: AAH73135.1 |
CCDSi | CCDS103.1 |
RefSeqi | NP_115691.1, NM_032315.2 |
3D structure databases
AlphaFoldDBi | Q9BSK2 |
SMRi | Q9BSK2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 124002, 43 interactors |
IntActi | Q9BSK2, 5 interactors |
MINTi | Q9BSK2 |
STRINGi | 9606.ENSP00000306328 |
Protein family/group databases
TCDBi | 2.A.29.10.7, the mitochondrial carrier (mc) family |
PTM databases
iPTMneti | Q9BSK2 |
PhosphoSitePlusi | Q9BSK2 |
Genetic variation databases
BioMutai | SLC25A33 |
DMDMi | 74752304 |
Proteomic databases
EPDi | Q9BSK2 |
jPOSTi | Q9BSK2 |
MassIVEi | Q9BSK2 |
MaxQBi | Q9BSK2 |
PaxDbi | Q9BSK2 |
PeptideAtlasi | Q9BSK2 |
PRIDEi | Q9BSK2 |
ProteomicsDBi | 78911 |
Protocols and materials databases
Antibodypediai | 53479, 75 antibodies from 18 providers |
DNASUi | 84275 |
Genome annotation databases
Ensembli | ENST00000302692.7; ENSP00000306328.5; ENSG00000171612.7 |
GeneIDi | 84275 |
KEGGi | hsa:84275 |
MANE-Selecti | ENST00000302692.7; ENSP00000306328.5; NM_032315.3; NP_115691.1 |
UCSCi | uc001apw.4, human |
Organism-specific databases
CTDi | 84275 |
DisGeNETi | 84275 |
GeneCardsi | SLC25A33 |
HGNCi | HGNC:29681, SLC25A33 |
HPAi | ENSG00000171612, Tissue enhanced (tongue) |
MIMi | 610816, gene |
neXtProti | NX_Q9BSK2 |
OpenTargetsi | ENSG00000171612 |
PharmGKBi | PA162403588 |
VEuPathDBi | HostDB:ENSG00000171612 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0757, Eukaryota |
GeneTreei | ENSGT00940000158954 |
HOGENOMi | CLU_015166_6_0_1 |
InParanoidi | Q9BSK2 |
OMAi | PTWAVYM |
OrthoDBi | 1372287at2759 |
PhylomeDBi | Q9BSK2 |
TreeFami | TF314220 |
Enzyme and pathway databases
PathwayCommonsi | Q9BSK2 |
SignaLinki | Q9BSK2 |
Miscellaneous databases
BioGRID-ORCSi | 84275, 50 hits in 1074 CRISPR screens |
ChiTaRSi | SLC25A33, human |
GenomeRNAii | 84275 |
Pharosi | Q9BSK2, Tbio |
PROi | PR:Q9BSK2 |
RNActi | Q9BSK2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000171612, Expressed in liver and 123 other tissues |
Genevisiblei | Q9BSK2, HS |
Family and domain databases
Gene3Di | 1.50.40.10, 2 hits |
InterProi | View protein in InterPro IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | S2533_HUMAN | |
Accessioni | Q9BSK2Primary (citable) accession number: Q9BSK2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2007 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families