UniProtKB - Q9BSB4 (ATGA1_HUMAN)
Protein
Autophagy-related protein 101
Gene
ATG101
Organism
Homo sapiens (Human)
Status
Functioni
Autophagy factor required for autophagosome formation. Stabilizes ATG13, protecting it from proteasomal degradation.2 Publications
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein-containing complex binding Source: UniProtKB
GO - Biological processi
- autophagosome assembly Source: UniProtKB
- macroautophagy Source: Reactome
- regulation of macroautophagy Source: Reactome
Keywordsi
Biological process | Autophagy |
Enzyme and pathway databases
PathwayCommonsi | Q9BSB4 |
Reactomei | R-HSA-1632852, Macroautophagy |
SIGNORi | Q9BSB4 |
Protein family/group databases
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Autophagy-related protein 101 |
Gene namesi | Name:ATG101 Synonyms:C12orf44 ORF Names:PP894 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25679, ATG101 |
MIMi | 615089, gene |
neXtProti | NX_Q9BSB4 |
VEuPathDBi | HostDB:ENSG00000123395.14 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Preautophagosomal structure 1 Publication
Note: Under starvation conditions, it is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation.1 Publication
Cytosol
- cytosol Source: Reactome
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
Other locations
- phagophore assembly site Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | H → S: Impairs interaction with ATG13; when associated with R-54. 1 Publication | 1 | |
Mutagenesisi | 54 | D → R: Impairs interaction with ATG13; when associated with S-31. 1 Publication | 1 | |
Mutagenesisi | 152 | I → D: Abolishes interaction with ATG13; when associated with D-153 and D-156. 1 Publication | 1 | |
Mutagenesisi | 153 | I → D: Abolishes interaction with ATG13; when associated with D-152 and D-156. 1 Publication | 1 | |
Mutagenesisi | 156 | V → D: Abolishes interaction with ATG13; when associated with D-152 and D-152. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 60673 |
OpenTargetsi | ENSG00000123395 |
PharmGKBi | PA143485374 |
Miscellaneous databases
Pharosi | Q9BSB4, Tbio |
Genetic variation databases
BioMutai | ATG101 |
DMDMi | 74732983 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000294322 | 1 – 218 | Autophagy-related protein 101Add BLAST | 218 |
Proteomic databases
EPDi | Q9BSB4 |
jPOSTi | Q9BSB4 |
MassIVEi | Q9BSB4 |
MaxQBi | Q9BSB4 |
PaxDbi | Q9BSB4 |
PeptideAtlasi | Q9BSB4 |
PRIDEi | Q9BSB4 |
ProteomicsDBi | 78873 |
PTM databases
iPTMneti | Q9BSB4 |
PhosphoSitePlusi | Q9BSB4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000123395, Expressed in stromal cell of endometrium and 232 other tissues |
ExpressionAtlasi | Q9BSB4, baseline and differential |
Genevisiblei | Q9BSB4, HS |
Organism-specific databases
HPAi | ENSG00000123395, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ9BSB4
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 121948, 60 interactors |
ComplexPortali | CPX-373, ULK1-ATG13-RB1CC1-ATG101 autophagy initiation complex |
CORUMi | Q9BSB4 |
DIPi | DIP-60653N |
IntActi | Q9BSB4, 45 interactors |
MINTi | Q9BSB4 |
STRINGi | 9606.ENSP00000338990 |
Miscellaneous databases
RNActi | Q9BSB4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9BSB4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 152 – 156 | Important for interaction with ATG131 Publication | 5 |
Sequence similaritiesi
Belongs to the ATG101 family.Curated
Phylogenomic databases
eggNOGi | KOG4493, Eukaryota |
GeneTreei | ENSGT00390000016511 |
InParanoidi | Q9BSB4 |
OMAi | MNARTQT |
OrthoDBi | 1539728at2759 |
PhylomeDBi | Q9BSB4 |
TreeFami | TF320996 |
Family and domain databases
InterProi | View protein in InterPro IPR012445, ATG101 |
PANTHERi | PTHR13292, PTHR13292, 1 hit |
Pfami | View protein in Pfam PF07855, ATG101, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q9BSB4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNCRSEVLEV SVEGRQVEEA MLAVLHTVLL HRSTGKFHYK KEGTYSIGTV
60 70 80 90 100
GTQDVDCDFI DFTYVRVSSE ELDRALRKVV GEFKDALRNS GGDGLGQMSL
110 120 130 140 150
EFYQKKKSRW PFSDECIPWE VWTVKVHVVA LATEQERQIC REKVGEKLCE
160 170 180 190 200
KIINIVEVMN RHEYLPKMPT QSEVDNVFDT GLRDVQPYLY KISFQITDAL
210
GTSVTTTMRR LIKDTLAL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VQD9 | F8VQD9_HUMAN | Autophagy-related protein 101 | ATG101 | 190 | Annotation score: | ||
H3BMZ4 | H3BMZ4_HUMAN | Autophagy-related protein 101 | ATG101 | 84 | Annotation score: | ||
F8VVA0 | F8VVA0_HUMAN | Autophagy-related protein 101 | ATG101 | 141 | Annotation score: |
Sequence cautioni
The sequence AAG17273 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 | I → T in BAB13907 (PubMed:14702039).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK021835 mRNA Translation: BAB13907.1 AF218031 mRNA Translation: AAG17273.1 Frameshift. AC025259 Genomic DNA No translation available. BC005151 mRNA Translation: AAH05151.1 BC009937 mRNA Translation: AAH09937.1 |
CCDSi | CCDS8820.1 |
RefSeqi | NP_001092143.1, NM_001098673.1 NP_068753.2, NM_021934.4 |
Genome annotation databases
Ensembli | ENST00000336854; ENSP00000338990; ENSG00000123395 |
GeneIDi | 60673 |
KEGGi | hsa:60673 |
UCSCi | uc001rzu.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK021835 mRNA Translation: BAB13907.1 AF218031 mRNA Translation: AAG17273.1 Frameshift. AC025259 Genomic DNA No translation available. BC005151 mRNA Translation: AAH05151.1 BC009937 mRNA Translation: AAH09937.1 |
CCDSi | CCDS8820.1 |
RefSeqi | NP_001092143.1, NM_001098673.1 NP_068753.2, NM_021934.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4WZG | X-ray | 1.90 | A | 1-218 | [»] | |
5C50 | X-ray | 1.63 | A | 1-198 | [»] | |
5XUY | X-ray | 2.20 | B/D | 1-218 | [»] | |
5XV1 | X-ray | 2.51 | B/D | 1-218 | [»] | |
5XV3 | X-ray | 2.57 | B/D | 1-218 | [»] | |
5XV4 | X-ray | 2.95 | B/D/F/H/J/L/N/P | 1-218 | [»] | |
5XV6 | X-ray | 2.46 | B | 1-218 | [»] | |
SMRi | Q9BSB4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121948, 60 interactors |
ComplexPortali | CPX-373, ULK1-ATG13-RB1CC1-ATG101 autophagy initiation complex |
CORUMi | Q9BSB4 |
DIPi | DIP-60653N |
IntActi | Q9BSB4, 45 interactors |
MINTi | Q9BSB4 |
STRINGi | 9606.ENSP00000338990 |
Protein family/group databases
TCDBi | 9.A.15.2.1, the autophagy-related phagophore-formation transporter (apt) family |
PTM databases
iPTMneti | Q9BSB4 |
PhosphoSitePlusi | Q9BSB4 |
Genetic variation databases
BioMutai | ATG101 |
DMDMi | 74732983 |
Proteomic databases
EPDi | Q9BSB4 |
jPOSTi | Q9BSB4 |
MassIVEi | Q9BSB4 |
MaxQBi | Q9BSB4 |
PaxDbi | Q9BSB4 |
PeptideAtlasi | Q9BSB4 |
PRIDEi | Q9BSB4 |
ProteomicsDBi | 78873 |
Protocols and materials databases
Antibodypediai | 43032, 84 antibodies |
DNASUi | 60673 |
Genome annotation databases
Ensembli | ENST00000336854; ENSP00000338990; ENSG00000123395 |
GeneIDi | 60673 |
KEGGi | hsa:60673 |
UCSCi | uc001rzu.5, human |
Organism-specific databases
CTDi | 60673 |
DisGeNETi | 60673 |
GeneCardsi | ATG101 |
HGNCi | HGNC:25679, ATG101 |
HPAi | ENSG00000123395, Low tissue specificity |
MIMi | 615089, gene |
neXtProti | NX_Q9BSB4 |
OpenTargetsi | ENSG00000123395 |
PharmGKBi | PA143485374 |
VEuPathDBi | HostDB:ENSG00000123395.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4493, Eukaryota |
GeneTreei | ENSGT00390000016511 |
InParanoidi | Q9BSB4 |
OMAi | MNARTQT |
OrthoDBi | 1539728at2759 |
PhylomeDBi | Q9BSB4 |
TreeFami | TF320996 |
Enzyme and pathway databases
PathwayCommonsi | Q9BSB4 |
Reactomei | R-HSA-1632852, Macroautophagy |
SIGNORi | Q9BSB4 |
Miscellaneous databases
BioGRID-ORCSi | 60673, 20 hits in 984 CRISPR screens |
ChiTaRSi | ATG101, human |
GeneWikii | Autophagy-related_protein_101 |
GenomeRNAii | 60673 |
Pharosi | Q9BSB4, Tbio |
PROi | PR:Q9BSB4 |
RNActi | Q9BSB4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000123395, Expressed in stromal cell of endometrium and 232 other tissues |
ExpressionAtlasi | Q9BSB4, baseline and differential |
Genevisiblei | Q9BSB4, HS |
Family and domain databases
InterProi | View protein in InterPro IPR012445, ATG101 |
PANTHERi | PTHR13292, PTHR13292, 1 hit |
Pfami | View protein in Pfam PF07855, ATG101, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATGA1_HUMAN | |
Accessioni | Q9BSB4Primary (citable) accession number: Q9BSB4 Secondary accession number(s): Q9HAE2, Q9HBN1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 10, 2007 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families