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Protein

DNA replication complex GINS protein SLD5

Gene

GINS4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA.1 Publication

GO - Biological processi

Keywordsi

Biological processDNA replication

Enzyme and pathway databases

ReactomeiR-HSA-176974 Unwinding of DNA

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein SLD5
Alternative name(s):
GINS complex subunit 4
Cleaved into the following chain:
Gene namesi
Name:GINS4
Synonyms:SLD5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000147536.11
HGNCiHGNC:28226 GINS4
MIMi610611 gene
neXtProtiNX_Q9BRT9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi84296
OpenTargetsiENSG00000147536
PharmGKBiPA145008337

Polymorphism and mutation databases

BioMutaiGINS4
DMDMi74732928

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003276201 – 223DNA replication complex GINS protein SLD5Add BLAST223
Initiator methionineiRemoved; alternateCombined sources
ChainiPRO_00004217942 – 223DNA replication complex GINS protein SLD5, N-terminally processedAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei2N-acetylthreonine; in DNA replication complex GINS protein SLD5, N-terminally processedCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BRT9
MaxQBiQ9BRT9
PaxDbiQ9BRT9
PeptideAtlasiQ9BRT9
PRIDEiQ9BRT9
ProteomicsDBi78835
78836 [Q9BRT9-2]

PTM databases

iPTMnetiQ9BRT9
PhosphoSitePlusiQ9BRT9

Expressioni

Inductioni

Significantly up-regulated in aggressive melanomas.1 Publication

Gene expression databases

BgeeiENSG00000147536 Expressed in 106 organ(s), highest expression level in oocyte
CleanExiHS_GINS4
ExpressionAtlasiQ9BRT9 baseline and differential
GenevisibleiQ9BRT9 HS

Organism-specific databases

HPAiHPA024663
HPA026580

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS1. GINS complex interacts with DNA primase in vitro.6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi124023, 46 interactors
CORUMiQ9BRT9
DIPiDIP-29334N
IntActiQ9BRT9, 19 interactors
MINTiQ9BRT9
STRINGi9606.ENSP00000276533

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BRT9
SMRiQ9BRT9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRT9

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 223Important for GINS complex assemblyAdd BLAST58

Sequence similaritiesi

Belongs to the GINS4/SLD5 family.Curated

Phylogenomic databases

eggNOGiKOG3176 Eukaryota
COG5086 LUCA
GeneTreeiENSGT00390000003246
HOGENOMiHOG000215319
HOVERGENiHBG059490
InParanoidiQ9BRT9
KOiK10735
OMAiVSIHRME
OrthoDBiEOG091G0ULR
PhylomeDBiQ9BRT9
TreeFamiTF105863

Family and domain databases

CDDicd11711 GINS_A_Sld5, 1 hit
InterProiView protein in InterPro
IPR021151 GINS_A
IPR036224 GINS_bundle-like_dom_sf
IPR008591 GINS_Sld5
IPR031633 SLD5_C
IPR038749 Sld5_GINS_A
PANTHERiPTHR21206 PTHR21206, 1 hit
PfamiView protein in Pfam
PF05916 Sld5, 1 hit
PF16922 SLD5_C, 1 hit
PIRSFiPIRSF007764 Sld5, 1 hit
SUPFAMiSSF158573 SSF158573, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BRT9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEEVDFLGQ DSDGGSEEVV LTPAELIERL EQAWMNEKFA PELLESKPEI
60 70 80 90 100
VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI
110 120 130 140 150
EKFFPHVLEK EKTRPEGEPS SLSPEELAFA REFMANTESY LKNVALKHMP
160 170 180 190 200
PNLQKVDLFR AVPKPDLDSY VFLRVRERQE NILVEPDTDE QRDYVIDLEK
210 220
GSQHLIRYKT IAPLVASGAV QLI
Length:223
Mass (Da):26,047
Last modified:June 1, 2001 - v1
Checksum:i8FAB619A21968BB9
GO
Isoform 2 (identifier: Q9BRT9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-100: I → V
     101-223: Missing.

Show »
Length:100
Mass (Da):11,844
Checksum:i2E8F496F2DC656B9
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RFF9E5RFF9_HUMAN
DNA replication complex GINS protei...
GINS4
192Annotation score:
E5RH56E5RH56_HUMAN
DNA replication complex GINS protei...
GINS4
104Annotation score:

Mass spectrometryi

Molecular mass is 98373±13 Da from positions 1 - 223. Determined by ESI. This is the measured mass for the GINS complex.1 Publication

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032738100I → V in isoform 2. 1 Publication1
Alternative sequenceiVSP_032739101 – 223Missing in isoform 2. 1 PublicationAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095334 mRNA Translation: BAG53029.1
AK313373 mRNA Translation: BAG36172.1
CH471080 Genomic DNA Translation: EAW63256.1
CH471080 Genomic DNA Translation: EAW63257.1
BC005995 mRNA Translation: AAH05995.1
BC027454 mRNA Translation: AAH27454.1
CCDSiCCDS6116.1 [Q9BRT9-1]
RefSeqiNP_115712.1, NM_032336.2 [Q9BRT9-1]
XP_005273716.1, XM_005273659.3 [Q9BRT9-1]
UniGeneiHs.656996

Genome annotation databases

EnsembliENST00000276533; ENSP00000276533; ENSG00000147536 [Q9BRT9-1]
ENST00000518671; ENSP00000428754; ENSG00000147536 [Q9BRT9-1]
ENST00000520710; ENSP00000429581; ENSG00000147536 [Q9BRT9-2]
GeneIDi84296
KEGGihsa:84296
UCSCiuc003xnx.3 human [Q9BRT9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK095334 mRNA Translation: BAG53029.1
AK313373 mRNA Translation: BAG36172.1
CH471080 Genomic DNA Translation: EAW63256.1
CH471080 Genomic DNA Translation: EAW63257.1
BC005995 mRNA Translation: AAH05995.1
BC027454 mRNA Translation: AAH27454.1
CCDSiCCDS6116.1 [Q9BRT9-1]
RefSeqiNP_115712.1, NM_032336.2 [Q9BRT9-1]
XP_005273716.1, XM_005273659.3 [Q9BRT9-1]
UniGeneiHs.656996

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E9XX-ray2.30D/H1-223[»]
2EHOX-ray3.00A/E/I11-213[»]
2Q9QX-ray2.36B/F1-223[»]
ProteinModelPortaliQ9BRT9
SMRiQ9BRT9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124023, 46 interactors
CORUMiQ9BRT9
DIPiDIP-29334N
IntActiQ9BRT9, 19 interactors
MINTiQ9BRT9
STRINGi9606.ENSP00000276533

PTM databases

iPTMnetiQ9BRT9
PhosphoSitePlusiQ9BRT9

Polymorphism and mutation databases

BioMutaiGINS4
DMDMi74732928

Proteomic databases

EPDiQ9BRT9
MaxQBiQ9BRT9
PaxDbiQ9BRT9
PeptideAtlasiQ9BRT9
PRIDEiQ9BRT9
ProteomicsDBi78835
78836 [Q9BRT9-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276533; ENSP00000276533; ENSG00000147536 [Q9BRT9-1]
ENST00000518671; ENSP00000428754; ENSG00000147536 [Q9BRT9-1]
ENST00000520710; ENSP00000429581; ENSG00000147536 [Q9BRT9-2]
GeneIDi84296
KEGGihsa:84296
UCSCiuc003xnx.3 human [Q9BRT9-1]

Organism-specific databases

CTDi84296
DisGeNETi84296
EuPathDBiHostDB:ENSG00000147536.11
GeneCardsiGINS4
HGNCiHGNC:28226 GINS4
HPAiHPA024663
HPA026580
MIMi610611 gene
neXtProtiNX_Q9BRT9
OpenTargetsiENSG00000147536
PharmGKBiPA145008337
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3176 Eukaryota
COG5086 LUCA
GeneTreeiENSGT00390000003246
HOGENOMiHOG000215319
HOVERGENiHBG059490
InParanoidiQ9BRT9
KOiK10735
OMAiVSIHRME
OrthoDBiEOG091G0ULR
PhylomeDBiQ9BRT9
TreeFamiTF105863

Enzyme and pathway databases

ReactomeiR-HSA-176974 Unwinding of DNA

Miscellaneous databases

ChiTaRSiGINS4 human
EvolutionaryTraceiQ9BRT9
GenomeRNAii84296
PROiPR:Q9BRT9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147536 Expressed in 106 organ(s), highest expression level in oocyte
CleanExiHS_GINS4
ExpressionAtlasiQ9BRT9 baseline and differential
GenevisibleiQ9BRT9 HS

Family and domain databases

CDDicd11711 GINS_A_Sld5, 1 hit
InterProiView protein in InterPro
IPR021151 GINS_A
IPR036224 GINS_bundle-like_dom_sf
IPR008591 GINS_Sld5
IPR031633 SLD5_C
IPR038749 Sld5_GINS_A
PANTHERiPTHR21206 PTHR21206, 1 hit
PfamiView protein in Pfam
PF05916 Sld5, 1 hit
PF16922 SLD5_C, 1 hit
PIRSFiPIRSF007764 Sld5, 1 hit
SUPFAMiSSF158573 SSF158573, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSLD5_HUMAN
AccessioniPrimary (citable) accession number: Q9BRT9
Secondary accession number(s): B2R8H5, D3DSY0, Q8N648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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