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Protein

Serine/threonine-protein kinase RIO1

Gene

RIOK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed:21081503).By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208ATPCombined sources1 Publication1
Binding sitei278ATP; via carbonyl oxygenCombined sources1 Publication1
Binding sitei280ATP; via amide nitrogenCombined sources1 Publication1
Active sitei324Proton acceptor1 Publication1 Publication1
Metal bindingi329MagnesiumCombined sources1 Publication1
Active sitei3414-aspartylphosphate intermediateCombined sources1 Publication1
Metal bindingi341MagnesiumCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

  • maturation of SSU-rRNA Source: UniProtKB
  • positive regulation of rRNA processing Source: UniProtKB
  • ribosomal small subunit biogenesis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Kinase, Serine/threonine-protein kinase, Transferase
Biological processRibosome biogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RIO1 (EC:2.7.11.11 Publication, EC:3.6.3.-)
Alternative name(s):
RIO kinase 1
Gene namesi
Name:RIOK1Imported
Synonyms:RIO1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000124784.8
HGNCiHGNC:18656 RIOK1
MIMi617753 gene
neXtProtiNX_Q9BRS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi324D → A: Abolishes autophosphorylation activity; enhances association with pre-40S ribosomal subunits; inhibits processing of 18S-E pre-rRNA to the mature 18S rRNA. 1 Publication1

Organism-specific databases

DisGeNETi83732
OpenTargetsiENSG00000124784
PharmGKBiPA134928236

Chemistry databases

ChEMBLiCHEMBL5975

Polymorphism and mutation databases

BioMutaiRIOK1
DMDMi56404949

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135261 – 568Serine/threonine-protein kinase RIO1Add BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21PhosphoserineCombined sources1
Modified residuei22PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BRS2
MaxQBiQ9BRS2
PaxDbiQ9BRS2
PeptideAtlasiQ9BRS2
PRIDEiQ9BRS2
ProteomicsDBi78823

PTM databases

iPTMnetiQ9BRS2
PhosphoSitePlusiQ9BRS2

Expressioni

Gene expression databases

BgeeiENSG00000124784 Expressed in 182 organ(s), highest expression level in testis
CleanExiHS_RIOK1
ExpressionAtlasiQ9BRS2 baseline and differential
GenevisibleiQ9BRS2 HS

Organism-specific databases

HPAiHPA017866
HPA051446

Interactioni

Subunit structurei

Associates with the precursor of the 40S ribosome subunit. Interacts (via its N-terminus) with PRMT5 (via its N-terminus) (PubMed:22072790, PubMed:21081503). Interacts with WDR77 (PubMed:22072790). Found in a PRMT5 complex composed of PRMT5, WDR77 and RIOK1 (PubMed:21081503). Interacts (via its C-terminus) with NCL; this interaction targets NCL for PRTM5 methylation (PubMed:21081503).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRMT5O147444EBI-7307838,EBI-351098

Protein-protein interaction databases

BioGridi123743, 55 interactors
IntActiQ9BRS2, 11 interactors
MINTiQ9BRS2
STRINGi9606.ENSP00000369162

Chemistry databases

BindingDBiQ9BRS2

Structurei

Secondary structure

1568
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BRS2
SMRiQ9BRS2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 479Protein kinaseAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2270 Eukaryota
COG1718 LUCA
GeneTreeiENSGT00390000004370
HOGENOMiHOG000166501
HOVERGENiHBG056997
InParanoidiQ9BRS2
KOiK07178
OMAiQEINGCI
OrthoDBiEOG091G064C
PhylomeDBiQ9BRS2
TreeFamiTF105831

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000687 RIO_kinase
IPR018935 RIO_kinase_CS
IPR017407 Ser/Thr_kinase_Rio1
PIRSFiPIRSF038147 Ser/Thr_PK_RIO1, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01245 RIO1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9BRS2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDYRRLLMSR VVPGQFDDAD SSDSENRDLK TVKEKDDILF EDLQDNVNEN
60 70 80 90 100
GEGEIEDEEE EGYDDDDDDW DWDEGVGKLA KGYVWNGGSN PQANRQTSDS
110 120 130 140 150
SSAKMSTPAD KVLRKFENKI NLDKLNVTDS VINKVTEKSR QKEADMYRIK
160 170 180 190 200
DKADRATVEQ VLDPRTRMIL FKMLTRGIIT EINGCISTGK EANVYHASTA
210 220 230 240 250
NGESRAIKIY KTSILVFKDR DKYVSGEFRF RHGYCKGNPR KMVKTWAEKE
260 270 280 290 300
MRNLIRLNTA EIPCPEPIML RSHVLVMSFI GKDDMPAPLL KNVQLSESKA
310 320 330 340 350
RELYLQVIQY MRRMYQDARL VHADLSEFNM LYHGGGVYII DVSQSVEHDH
360 370 380 390 400
PHALEFLRKD CANVNDFFMR HSVAVMTVRE LFEFVTDPSI THENMDAYLS
410 420 430 440 450
KAMEIASQRT KEERSSQDHV DEEVFKRAYI PRTLNEVKNY ERDMDIIMKL
460 470 480 490 500
KEEDMAMNAQ QDNILYQTVT GLKKDLSGVQ KVPALLENQV EERTCSDSED
510 520 530 540 550
IGSSECSDTD SEEQGDHARP KKHTTDPDID KKERKKMVKE AQREKRKNKI
560
PKHVKKRKEK TAKTKKGK
Length:568
Mass (Da):65,583
Last modified:March 1, 2004 - v2
Checksum:i5730BFC3798F5190
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PFQ8E9PFQ8_HUMAN
Serine/threonine-protein kinase RIO...
RIOK1
95Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061777375V → I. Corresponds to variant dbSNP:rs56067778Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK054568 mRNA Translation: BAB70761.1
AK314467 mRNA Translation: BAG37075.1
CH471087 Genomic DNA Translation: EAW55210.1
BC006104 mRNA Translation: AAH06104.2
AL834277 mRNA Translation: CAD38952.1
CCDSiCCDS4500.1
RefSeqiNP_113668.2, NM_031480.2
UniGeneiHs.437474

Genome annotation databases

EnsembliENST00000379834; ENSP00000369162; ENSG00000124784
GeneIDi83732
KEGGihsa:83732
UCSCiuc003mxn.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK054568 mRNA Translation: BAB70761.1
AK314467 mRNA Translation: BAG37075.1
CH471087 Genomic DNA Translation: EAW55210.1
BC006104 mRNA Translation: AAH06104.2
AL834277 mRNA Translation: CAD38952.1
CCDSiCCDS4500.1
RefSeqiNP_113668.2, NM_031480.2
UniGeneiHs.437474

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OTPX-ray2.70A143-494[»]
6G5Ielectron microscopy3.50z1-568[»]
ProteinModelPortaliQ9BRS2
SMRiQ9BRS2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123743, 55 interactors
IntActiQ9BRS2, 11 interactors
MINTiQ9BRS2
STRINGi9606.ENSP00000369162

Chemistry databases

BindingDBiQ9BRS2
ChEMBLiCHEMBL5975

PTM databases

iPTMnetiQ9BRS2
PhosphoSitePlusiQ9BRS2

Polymorphism and mutation databases

BioMutaiRIOK1
DMDMi56404949

Proteomic databases

EPDiQ9BRS2
MaxQBiQ9BRS2
PaxDbiQ9BRS2
PeptideAtlasiQ9BRS2
PRIDEiQ9BRS2
ProteomicsDBi78823

Protocols and materials databases

DNASUi83732
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379834; ENSP00000369162; ENSG00000124784
GeneIDi83732
KEGGihsa:83732
UCSCiuc003mxn.4 human

Organism-specific databases

CTDi83732
DisGeNETi83732
EuPathDBiHostDB:ENSG00000124784.8
GeneCardsiRIOK1
HGNCiHGNC:18656 RIOK1
HPAiHPA017866
HPA051446
MIMi617753 gene
neXtProtiNX_Q9BRS2
OpenTargetsiENSG00000124784
PharmGKBiPA134928236
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2270 Eukaryota
COG1718 LUCA
GeneTreeiENSGT00390000004370
HOGENOMiHOG000166501
HOVERGENiHBG056997
InParanoidiQ9BRS2
KOiK07178
OMAiQEINGCI
OrthoDBiEOG091G064C
PhylomeDBiQ9BRS2
TreeFamiTF105831

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRSiRIOK1 human
GenomeRNAii83732
PROiPR:Q9BRS2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124784 Expressed in 182 organ(s), highest expression level in testis
CleanExiHS_RIOK1
ExpressionAtlasiQ9BRS2 baseline and differential
GenevisibleiQ9BRS2 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000687 RIO_kinase
IPR018935 RIO_kinase_CS
IPR017407 Ser/Thr_kinase_Rio1
PIRSFiPIRSF038147 Ser/Thr_PK_RIO1, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01245 RIO1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIOK1_HUMAN
AccessioniPrimary (citable) accession number: Q9BRS2
Secondary accession number(s): B2RB28, Q8NDC8, Q96NV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2004
Last modified: September 12, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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