Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

45 kDa calcium-binding protein

Gene

SDF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.By similarity
Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi111 – 1221PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi150 – 1612PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi209 – 2203PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi246 – 2574PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi291 – 3025PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi327 – 3386PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: BHF-UCL
  • identical protein binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Biological processExocytosis
LigandCalcium, Metal-binding

Protein family/group databases

TCDBi8.A.82.3.1 the calmodulin calcium binding protein (calmodulin) family

Names & Taxonomyi

Protein namesi
Recommended name:
45 kDa calcium-binding protein
Short name:
Cab45
Alternative name(s):
Stromal cell-derived factor 4
Short name:
SDF-4
Gene namesi
Name:SDF4
Synonyms:CAB45
ORF Names:PSEC0034
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000078808.16
HGNCiHGNC:24188 SDF4
MIMi614282 gene
neXtProtiNX_Q9BRK5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi257E → Q: Does not affect calcium-binding. 1 Publication1
Mutagenesisi302E → Q: Inhibits calcium-binding. 1 Publication1
Mutagenesisi338E → Q: Does not affect calcium-binding. 1 Publication1

Organism-specific databases

DisGeNETi51150
OpenTargetsiENSG00000078808
PharmGKBiPA142670940

Polymorphism and mutation databases

BioMutaiSDF4
DMDMi21263447

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
ChainiPRO_000000415637 – 36245 kDa calcium-binding proteinAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei99PhosphoserineCombined sources1
Modified residuei193PhosphothreonineCombined sources1
Modified residuei217PhosphothreonineCombined sources1
Modified residuei265PhosphothreonineCombined sources1
Modified residuei299PhosphothreonineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9BRK5
MaxQBiQ9BRK5
PaxDbiQ9BRK5
PeptideAtlasiQ9BRK5
PRIDEiQ9BRK5
ProteomicsDBi78779
78780 [Q9BRK5-2]
78781 [Q9BRK5-3]
78782 [Q9BRK5-4]
78783 [Q9BRK5-5]
78784 [Q9BRK5-6]
TopDownProteomicsiQ9BRK5-4 [Q9BRK5-4]

PTM databases

iPTMnetiQ9BRK5
PhosphoSitePlusiQ9BRK5

Expressioni

Tissue specificityi

Ubiquitous. Isoform 5 is expressed in pancreas.1 Publication

Gene expression databases

BgeeiENSG00000078808 Expressed in 216 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_SDF4
ExpressionAtlasiQ9BRK5 baseline and differential
GenevisibleiQ9BRK5 HS

Organism-specific databases

HPAiCAB015227
HPA011249

Interactioni

Subunit structurei

Isoform 5 interacts with STXBP1; the interaction is enhanced in presence of calcium. Isoform 5 interacts with STX3.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119334, 91 interactors
IntActiQ9BRK5, 50 interactors
MINTiQ9BRK5
STRINGi9606.ENSP00000353094

Structurei

3D structure databases

ProteinModelPortaliQ9BRK5
SMRiQ9BRK5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 133EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini137 – 172EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini197 – 232EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini233 – 268EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Domaini278 – 313EF-hand 5PROSITE-ProRule annotationAdd BLAST36
Domaini314 – 349EF-hand 6PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 362Necessary for intracellular retention in Golgi apparatus lumenBy similarityAdd BLAST54

Domaini

Binds calcium via its EF-hands (By similarity). Isoform 5 binds calcium.By similarity

Sequence similaritiesi

Belongs to the CREC family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4251 Eukaryota
ENOG410XSH7 LUCA
GeneTreeiENSGT00550000074546
HOVERGENiHBG097344
InParanoidiQ9BRK5
KOiK19934
OMAiAKQMIAI
OrthoDBiEOG091G0GSA
PhylomeDBiQ9BRK5
TreeFamiTF314849

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 5 hits
SUPFAMiSSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 5 hits
PS50222 EF_HAND_2, 5 hits

Sequences (6+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BRK5-1) [UniParc]FASTAAdd to basket
Also known as: Cab45a, Cab45-G

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVWPWVAMAS RWGPLIGLAP CCLWLLGAVL LMDASARPAN HSSTRERVAN
60 70 80 90 100
REENEILPPD HLNGVKLEMD GHLNRGFHQE VFLGKDLGGF DEDAEPRRSR
110 120 130 140 150
RKLMVIFSKV DVNTDRKISA KEMQRWIMEK TAEHFQEAME ESKTHFRAVD
160 170 180 190 200
PDGDGHVSWD EYKVKFLASK GHSEKEVADA IRLNEELKVD EETQEVLENL
210 220 230 240 250
KDRWYQADSP PADLLLTEEE FLSFLHPEHS RGMLRFMVKE IVRDLDQDGD
260 270 280 290 300
KQLSVPEFIS LPVGTVENQQ GQDIDDNWVK DRKKEFEELI DSNHDGIVTA
310 320 330 340 350
EELESYMDPM NEYNALNEAK QMIAVADENQ NHHLEPEEVL KYSEFFTGSK
360
LVDYARSVHE EF
Length:362
Mass (Da):41,807
Last modified:June 1, 2001 - v1
Checksum:i440C6990149AAE2C
GO
Isoform 2 (identifier: Q9BRK5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Show »
Length:259
Mass (Da):30,197
Checksum:i3745A23BB8FF6D40
GO
Isoform 3 (identifier: Q9BRK5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     193-254: TQEVLENLKD...LDQDGDKQLS → SCAPLSTGSP...GDRPGPGPGR
     255-362: Missing.

Note: No experimental confirmation available.
Show »
Length:254
Mass (Da):27,826
Checksum:i77FACCE59B472125
GO
Isoform 4 (identifier: Q9BRK5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     193-202: TQEVLENLKD → RHGPPGPRAL
     203-362: Missing.

Note: No experimental confirmation available.
Show »
Length:202
Mass (Da):23,031
Checksum:i5F208F14504756F7
GO
Isoform 5 (identifier: Q9BRK5-5) [UniParc]FASTAAdd to basket
Also known as: Cab45b, Cab45-C

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: Missing.

Show »
Length:130
Mass (Da):15,139
Checksum:i22545D121BB56827
GO
Isoform 6 (identifier: Q9BRK5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     305-362: SYMDPMNEYN...DYARSVHEEF → NVPTLPLQPI...PLARRATWTP

Show »
Length:348
Mass (Da):39,608
Checksum:i1832BC4741A9506A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V1E2G3V1E2_HUMAN
45 kDa calcium-binding protein
SDF4 hCG_19193
254Annotation score:
H0Y3T6H0Y3T6_HUMAN
45 kDa calcium-binding protein
SDF4
243Annotation score:

Sequence cautioni

The sequence AAL75950 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78H → Q in AAL75950 (Ref. 2) Curated1
Sequence conflicti86D → N in AAL75950 (Ref. 2) Curated1
Sequence conflicti89G → C in AAL75950 (Ref. 2) Curated1
Sequence conflicti92E → R in AAL75950 (Ref. 2) Curated1
Sequence conflicti94A → T in AAL75950 (Ref. 2) Curated1
Sequence conflicti97R → P in AAL75950 (Ref. 2) Curated1
Sequence conflicti117K → R in BAC11563 (PubMed:16303743).Curated1
Sequence conflicti133E → G in BAC11563 (PubMed:16303743).Curated1
Sequence conflicti150D → G in AAL75950 (Ref. 2) Curated1
Sequence conflicti188K → R in BAG37898 (PubMed:14702039).Curated1
Sequence conflicti193T → A in BAD96241 (Ref. 7) Curated1
Sequence conflicti234L → P in BAD96799 (Ref. 7) Curated1
Sequence conflicti281 – 282DR → KK in AAL75950 (Ref. 2) Curated2
Sequence conflicti286F → L in AAL75950 (Ref. 2) Curated1
Sequence conflicti321Q → R in BAD96799 (Ref. 7) Curated1
Sequence conflicti356R → S in AAL75950 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04865950N → D. Corresponds to variant dbSNP:rs12745364Ensembl.1
Natural variantiVAR_035461148A → T in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs766752243Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0374841 – 232Missing in isoform 5. CuratedAdd BLAST232
Alternative sequenceiVSP_0374851 – 103Missing in isoform 2. 1 PublicationAdd BLAST103
Alternative sequenceiVSP_037486193 – 254TQEVL…DKQLS → SCAPLSTGSPGEPEGPLVPG GQPPCRPAADGGGVPVGPPP RAQPGNAQVHGEGDRPGPGP GR in isoform 3. 1 PublicationAdd BLAST62
Alternative sequenceiVSP_037487193 – 202TQEVLENLKD → RHGPPGPRAL in isoform 4. 1 Publication10
Alternative sequenceiVSP_037488203 – 362Missing in isoform 4. 1 PublicationAdd BLAST160
Alternative sequenceiVSP_037489255 – 362Missing in isoform 3. 1 PublicationAdd BLAST108
Alternative sequenceiVSP_040559305 – 362SYMDP…VHEEF → NVPTLPLQPIGTLNSHFVRL AAELGGGKATLTCAPLARRA TWTP in isoform 6. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L79912 mRNA Translation: AAL40084.1
AF132749 mRNA Translation: AAL75950.1 Frameshift.
AF153686 mRNA Translation: AAD51612.1
AF178986 mRNA Translation: AAF44350.1
AK027277 mRNA Translation: BAB55012.1
AK299810 mRNA Translation: BAG61683.1
AK315517 mRNA Translation: BAG37898.1
AK075352 mRNA Translation: BAC11563.1
AK222521 mRNA Translation: BAD96241.1
AK223079 mRNA Translation: BAD96799.1
AL162741 Genomic DNA No translation available.
CH471183 Genomic DNA Translation: EAW56272.1
CH471183 Genomic DNA Translation: EAW56273.1
BC006211 mRNA Translation: AAH06211.1
BC007625 mRNA Translation: AAH07625.1
BC008917 mRNA Translation: AAH08917.1
BC011244 mRNA Translation: AAH11244.1
BC022375 mRNA Translation: AAH22375.1
CCDSiCCDS12.1 [Q9BRK5-6]
CCDS30553.1 [Q9BRK5-1]
RefSeqiNP_057260.2, NM_016176.3 [Q9BRK5-1]
NP_057631.1, NM_016547.2 [Q9BRK5-6]
XP_011539858.1, XM_011541556.1 [Q9BRK5-4]
UniGeneiHs.42806

Genome annotation databases

EnsembliENST00000263741; ENSP00000263741; ENSG00000078808 [Q9BRK5-6]
ENST00000360001; ENSP00000353094; ENSG00000078808 [Q9BRK5-1]
GeneIDi51150
KEGGihsa:51150
UCSCiuc001adh.5 human [Q9BRK5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L79912 mRNA Translation: AAL40084.1
AF132749 mRNA Translation: AAL75950.1 Frameshift.
AF153686 mRNA Translation: AAD51612.1
AF178986 mRNA Translation: AAF44350.1
AK027277 mRNA Translation: BAB55012.1
AK299810 mRNA Translation: BAG61683.1
AK315517 mRNA Translation: BAG37898.1
AK075352 mRNA Translation: BAC11563.1
AK222521 mRNA Translation: BAD96241.1
AK223079 mRNA Translation: BAD96799.1
AL162741 Genomic DNA No translation available.
CH471183 Genomic DNA Translation: EAW56272.1
CH471183 Genomic DNA Translation: EAW56273.1
BC006211 mRNA Translation: AAH06211.1
BC007625 mRNA Translation: AAH07625.1
BC008917 mRNA Translation: AAH08917.1
BC011244 mRNA Translation: AAH11244.1
BC022375 mRNA Translation: AAH22375.1
CCDSiCCDS12.1 [Q9BRK5-6]
CCDS30553.1 [Q9BRK5-1]
RefSeqiNP_057260.2, NM_016176.3 [Q9BRK5-1]
NP_057631.1, NM_016547.2 [Q9BRK5-6]
XP_011539858.1, XM_011541556.1 [Q9BRK5-4]
UniGeneiHs.42806

3D structure databases

ProteinModelPortaliQ9BRK5
SMRiQ9BRK5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119334, 91 interactors
IntActiQ9BRK5, 50 interactors
MINTiQ9BRK5
STRINGi9606.ENSP00000353094

Protein family/group databases

TCDBi8.A.82.3.1 the calmodulin calcium binding protein (calmodulin) family

PTM databases

iPTMnetiQ9BRK5
PhosphoSitePlusiQ9BRK5

Polymorphism and mutation databases

BioMutaiSDF4
DMDMi21263447

Proteomic databases

EPDiQ9BRK5
MaxQBiQ9BRK5
PaxDbiQ9BRK5
PeptideAtlasiQ9BRK5
PRIDEiQ9BRK5
ProteomicsDBi78779
78780 [Q9BRK5-2]
78781 [Q9BRK5-3]
78782 [Q9BRK5-4]
78783 [Q9BRK5-5]
78784 [Q9BRK5-6]
TopDownProteomicsiQ9BRK5-4 [Q9BRK5-4]

Protocols and materials databases

DNASUi51150
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263741; ENSP00000263741; ENSG00000078808 [Q9BRK5-6]
ENST00000360001; ENSP00000353094; ENSG00000078808 [Q9BRK5-1]
GeneIDi51150
KEGGihsa:51150
UCSCiuc001adh.5 human [Q9BRK5-1]

Organism-specific databases

CTDi51150
DisGeNETi51150
EuPathDBiHostDB:ENSG00000078808.16
GeneCardsiSDF4
HGNCiHGNC:24188 SDF4
HPAiCAB015227
HPA011249
MIMi614282 gene
neXtProtiNX_Q9BRK5
OpenTargetsiENSG00000078808
PharmGKBiPA142670940
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4251 Eukaryota
ENOG410XSH7 LUCA
GeneTreeiENSGT00550000074546
HOVERGENiHBG097344
InParanoidiQ9BRK5
KOiK19934
OMAiAKQMIAI
OrthoDBiEOG091G0GSA
PhylomeDBiQ9BRK5
TreeFamiTF314849

Miscellaneous databases

ChiTaRSiSDF4 human
GeneWikiiSDF4
GenomeRNAii51150
PROiPR:Q9BRK5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000078808 Expressed in 216 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_SDF4
ExpressionAtlasiQ9BRK5 baseline and differential
GenevisibleiQ9BRK5 HS

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 5 hits
SUPFAMiSSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 5 hits
PS50222 EF_HAND_2, 5 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCAB45_HUMAN
AccessioniPrimary (citable) accession number: Q9BRK5
Secondary accession number(s): B1AME5
, B1AME6, B2RDF1, B4DSM1, Q53G52, Q53HQ9, Q8NBQ3, Q96AA1, Q9NZP7, Q9UN53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again