VPS25

Functionⁱ

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.1 Publication

GO - Molecular functionⁱ

protein homodimerization activity
Source: UniProtKB
Inferred from physical interactionⁱ
- 14519844
protein N-terminus binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 16973552
structural molecule activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

endosomal transport Source: Reactome
macroautophagy
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 20588296
multivesicular body assembly
Source: ParkinsonsUK-UCL
Traceable author statementⁱ
- 20588296
negative regulation of epidermal growth factor-activated receptor activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16973552
protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Protein transport, Transcription, Transcription regulation, Transport

Biological process

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

Reactomeⁱ	R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Reactomeⁱ

R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Vacuolar protein-sorting-associated protein 25 Short name: hVps25 Alternative name(s): Dermal papilla-derived protein 9 ELL-associated protein of 20 kDa ESCRT-II complex subunit VPS25
Gene namesⁱ	Name:VPS25 Synonyms:DERP9, EAP20
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 17

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000131475.6
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:28122 VPS25
MIMⁱ	610907 gene
neXtProtⁱ	NX_Q9BRG1

Keywords - Cellular componentⁱ

Cytoplasm, Endosome, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	124	V → E: Abolishes binding to CHMP6. 1 Publication		1
Mutagenesisⁱ	126	T → K: Abolishes binding to CHMP6. 1 Publication		1

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000131475
PharmGKBⁱ	PA142670614

Polymorphism and mutation databases

DMDMⁱ	73920459

DMDMⁱ

73920459

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000215216	1 – 176	Vacuolar protein-sorting-associated protein 25Add BLAST		176

Proteomic databases

EPDⁱ	Q9BRG1
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9BRG1
PaxDbⁱ	Q9BRG1
PeptideAtlas More...PeptideAtlasⁱ	Q9BRG1
PRIDEⁱ	Q9BRG1
ProteomicsDBⁱ	78762

PTM databases

iPTMnetⁱ	Q9BRG1
PhosphoSitePlusⁱ	Q9BRG1

Expressionⁱ

Tissue specificityⁱ

Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000131475 Expressed in 193 organ(s), highest expression level in cortex of kidney
CleanExⁱ	HS_VPS25
ExpressionAtlasⁱ	Q9BRG1 baseline and differential
Genevisibleⁱ	Q9BRG1 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB004978 HPA052217 HPA057284

HPAⁱ

CAB004978
HPA052217
HPA057284

Interactionⁱ

Subunit structureⁱ

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half).By similarity7 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
BEND5	Q7L4P6	3	EBI-741945,EBI-724373
CHMP6	Q96FZ7	6	EBI-741945,EBI-1049648
CRLF3	Q8IUI8	7	EBI-741945,EBI-2872414
REL	Q04864	3	EBI-741945,EBI-307352
SNF8	Q96H20	11	EBI-741945,EBI-747719
TRIM27	P14373	5	EBI-741945,EBI-719493

GO - Molecular functionⁱ

protein homodimerization activity
Source: UniProtKB
Inferred from physical interactionⁱ
- 14519844
protein N-terminus binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 16973552

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	124039, 38 interactors
CORUMⁱ	Q9BRG1
IntActⁱ	Q9BRG1, 20 interactors
Molecular INTeraction database More...MINTⁱ	Q9BRG1
STRINGⁱ	9606.ENSP00000253794

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	9 – 12	Combined sources	4
Helixⁱ	14 – 17	Combined sources	4
Helixⁱ	23 – 44	Combined sources	22
Beta strandⁱ	48 – 50	Combined sources	3
Helixⁱ	51 – 55	Combined sources	5
Beta strandⁱ	60 – 62	Combined sources	3
Turnⁱ	63 – 66	Combined sources	4
Helixⁱ	71 – 84	Combined sources	14
Beta strandⁱ	86 – 89	Combined sources	4
Beta strandⁱ	91 – 99	Combined sources	9
Helixⁱ	104 – 116	Combined sources	13
Turnⁱ	117 – 119	Combined sources	3
Beta strandⁱ	123 – 125	Combined sources	3
Helixⁱ	127 – 132	Combined sources	6
Turnⁱ	135 – 138	Combined sources	4
Turnⁱ	140 – 143	Combined sources	4
Helixⁱ	146 – 158	Combined sources	13
Beta strandⁱ	161 – 165	Combined sources	5
Turnⁱ	167 – 169	Combined sources	3
Beta strandⁱ	172 – 175	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9BRG1
SMRⁱ	Q9BRG1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9BRG1

EvolutionaryTraceⁱ

Q9BRG1

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the VPS25 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG4068 Eukaryota ENOG4111F6Y LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000014892
HOGENOMⁱ	HOG000191978
HOVERGENⁱ	HBG080015
InParanoidⁱ	Q9BRG1
KEGG Orthology (KO) More...KOⁱ	K12189
OMAⁱ	PPFFTIQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0PIU
PhylomeDBⁱ	Q9BRG1
TreeFamⁱ	TF317731

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit 1.10.10.570, 1 hit
InterProⁱ	View protein in InterPro IPR008570 ESCRT-II_cplx_Vps25-sub IPR014041 ESCRT-II_cplx_Vps25-sub_N IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
PANTHERⁱ	PTHR13149 PTHR13149, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF05871 ESCRT-II, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 2 hits

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show all Align All

Q9BRG1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT 
        60         70         80         90        100
VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM 
       110        120        130        140        150
WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL 
       160        170 
RALQALQQEH KAEIITVSDG RGVKFF

Length:176

Mass (Da):20,748

Last modified:June 1, 2001 - v1

Checksum:ⁱ34963A53C3DA4DD5

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

K7EKV4	K7EKV4_HUMAN	Vacuolar protein-sorting-associated... Vacuolar protein-sorting-associated protein 25	VPS25	111	Annotation score:
K7EP45	K7EP45_HUMAN	Vacuolar protein-sorting-associated... Vacuolar protein-sorting-associated protein 25	VPS25	99	Annotation score:
K7ENE3	K7ENE3_HUMAN	Vacuolar protein-sorting-associated... Vacuolar protein-sorting-associated protein 25	VPS25	108	Annotation score:

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_048940	76	I → V. Corresponds to variant dbSNP:rs34494804Ensembl.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB014763 mRNA Translation: BAB87804.1 AK312092 mRNA Translation: BAG35028.1 CH471152 Genomic DNA Translation: EAW60879.1 BC006282 mRNA Translation: AAH06282.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS11438.1
NCBI Reference Sequences More...RefSeqⁱ	NP_115729.1, NM_032353.3
UniGeneⁱ	Hs.500165

Genome annotation databases

Ensemblⁱ	ENST00000253794; ENSP00000253794; ENSG00000131475
GeneIDⁱ	84313
KEGGⁱ	hsa:84313
UCSC genome browser More...UCSCⁱ	uc002ibi.5 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9BRG1	Vacuolar protein sorting 25 (Yeast), isoform CRA_a		HUMAN		176	UniRef100_Q9BRG1
VPS25 isoform 1		PANTR		176
Vacuolar protein sorting 25 homolog		CALJA		176
Vacuolar protein sorting 25 homolog		MACMU		176
Vacuolar protein sorting 25 homolog		MACFA		176
+17

Protein	Similar proteins		Species	Score	Length	Source
Q9BRG1	Vacuolar protein-sorting-associated protein 25		MOUSE		176	UniRef90_Q9BRG1
Vacuolar protein-sorting-associated protein 25		RAT		176
Vacuolar protein sorting 25 (Yeast), isoform CRA_a		HUMAN		176
VPS25 isoform 1		PANTR		176
Vacuolar protein sorting 25 homolog		CALJA		176
+84

Protein	Similar proteins		Species	Score	Length	Source
Q9BRG1	Vacuolar protein-sorting-associated protein 25		MOUSE		176	UniRef50_Q9BRG1
Vacuolar protein-sorting-associated protein 25		RAT		176
Vacuolar protein-sorting-associated protein 25		BOVIN		176
Vacuolar protein sorting 25 (Yeast), isoform CRA_a		HUMAN		176
VPS25 isoform 1		PANTR		176
+98

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB014763 mRNA Translation: BAB87804.1 AK312092 mRNA Translation: BAG35028.1 CH471152 Genomic DNA Translation: EAW60879.1 BC006282 mRNA Translation: AAH06282.1
CCDSⁱ	CCDS11438.1
RefSeqⁱ	NP_115729.1, NM_032353.3
UniGeneⁱ	Hs.500165

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2ZME	X-ray	2.90	C/D	1-102	[»]
	3CUQ	X-ray	2.61	C/D	1-176	[»]
	3HTU	X-ray	2.00	A/C/E/G	102-176	[»]
ProteinModelPortalⁱ	Q9BRG1
SMRⁱ	Q9BRG1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	124039, 38 interactors
CORUMⁱ	Q9BRG1
IntActⁱ	Q9BRG1, 20 interactors
MINTⁱ	Q9BRG1
STRINGⁱ	9606.ENSP00000253794

PTM databases

iPTMnetⁱ	Q9BRG1
PhosphoSitePlusⁱ	Q9BRG1

Polymorphism and mutation databases

DMDMⁱ	73920459

DMDMⁱ

73920459

Proteomic databases

EPDⁱ	Q9BRG1
MaxQBⁱ	Q9BRG1
PaxDbⁱ	Q9BRG1
PeptideAtlasⁱ	Q9BRG1
PRIDEⁱ	Q9BRG1
ProteomicsDBⁱ	78762

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	84313
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000253794; ENSP00000253794; ENSG00000131475
GeneIDⁱ	84313
KEGGⁱ	hsa:84313
UCSCⁱ	uc002ibi.5 human

Organism-specific databases

CTDⁱ	84313
EuPathDBⁱ	HostDB:ENSG00000131475.6
GeneCardsⁱ	VPS25
HGNCⁱ	HGNC:28122 VPS25
HPAⁱ	CAB004978 HPA052217 HPA057284
MIMⁱ	610907 gene
neXtProtⁱ	NX_Q9BRG1
OpenTargetsⁱ	ENSG00000131475
PharmGKBⁱ	PA142670614
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG4068 Eukaryota ENOG4111F6Y LUCA
GeneTreeⁱ	ENSGT00390000014892
HOGENOMⁱ	HOG000191978
HOVERGENⁱ	HBG080015
InParanoidⁱ	Q9BRG1
KOⁱ	K12189
OMAⁱ	PPFFTIQ
OrthoDBⁱ	EOG091G0PIU
PhylomeDBⁱ	Q9BRG1
TreeFamⁱ	TF317731

Enzyme and pathway databases

Reactomeⁱ	R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Reactomeⁱ

R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRSⁱ	VPS25 human
EvolutionaryTraceⁱ	Q9BRG1
GeneWikiⁱ	VPS25
GenomeRNAiⁱ	84313
Protein Ontology More...PROⁱ	PR:Q9BRG1
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000131475 Expressed in 193 organ(s), highest expression level in cortex of kidney
CleanExⁱ	HS_VPS25
ExpressionAtlasⁱ	Q9BRG1 baseline and differential
Genevisibleⁱ	Q9BRG1 HS

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit 1.10.10.570, 1 hit
InterProⁱ	View protein in InterPro IPR008570 ESCRT-II_cplx_Vps25-sub IPR014041 ESCRT-II_cplx_Vps25-sub_N IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
PANTHERⁱ	PTHR13149 PTHR13149, 1 hit
Pfamⁱ	View protein in Pfam PF05871 ESCRT-II, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VPS25_HUMAN
Accessionⁱ	Q9BRG1Primary (citable) accession number: Q9BRG1 Secondary accession number(s): B2R581
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
	Last sequence update:	June 1, 2001
	Last modified:	November 7, 2018
	This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

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UniRef

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UniProtKB - Q9BRG1 (VPS25_HUMAN)

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Vacuolar protein-sorting-associated protein 25

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endosome

Nucleus

Other locations

Cytosol

Endosome

Extracellular region or secreted

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ