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Protein

Vacuolar protein-sorting-associated protein 25

Gene

VPS25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein N-terminus binding Source: UniProtKB
  • structural molecule activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processProtein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 25
Short name:
hVps25
Alternative name(s):
Dermal papilla-derived protein 9
ELL-associated protein of 20 kDa
ESCRT-II complex subunit VPS25
Gene namesi
Name:VPS25
Synonyms:DERP9, EAP20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000131475.6
HGNCiHGNC:28122 VPS25
MIMi610907 gene
neXtProtiNX_Q9BRG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124V → E: Abolishes binding to CHMP6. 1 Publication1
Mutagenesisi126T → K: Abolishes binding to CHMP6. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000131475
PharmGKBiPA142670614

Polymorphism and mutation databases

DMDMi73920459

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152161 – 176Vacuolar protein-sorting-associated protein 25Add BLAST176

Proteomic databases

EPDiQ9BRG1
MaxQBiQ9BRG1
PaxDbiQ9BRG1
PeptideAtlasiQ9BRG1
PRIDEiQ9BRG1
ProteomicsDBi78762

PTM databases

iPTMnetiQ9BRG1
PhosphoSitePlusiQ9BRG1

Expressioni

Tissue specificityi

Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.1 Publication

Gene expression databases

BgeeiENSG00000131475 Expressed in 193 organ(s), highest expression level in cortex of kidney
CleanExiHS_VPS25
ExpressionAtlasiQ9BRG1 baseline and differential
GenevisibleiQ9BRG1 HS

Organism-specific databases

HPAiCAB004978
HPA052217
HPA057284

Interactioni

Subunit structurei

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half).By similarity7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi124039, 38 interactors
CORUMiQ9BRG1
IntActiQ9BRG1, 20 interactors
MINTiQ9BRG1
STRINGi9606.ENSP00000253794

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BRG1
SMRiQ9BRG1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BRG1

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS25 family.Curated

Phylogenomic databases

eggNOGiKOG4068 Eukaryota
ENOG4111F6Y LUCA
GeneTreeiENSGT00390000014892
HOGENOMiHOG000191978
HOVERGENiHBG080015
InParanoidiQ9BRG1
KOiK12189
OMAiPPFFTIQ
OrthoDBiEOG091G0PIU
PhylomeDBiQ9BRG1
TreeFamiTF317731

Family and domain databases

Gene3Di1.10.10.10, 1 hit
1.10.10.570, 1 hit
InterProiView protein in InterPro
IPR008570 ESCRT-II_cplx_Vps25-sub
IPR014041 ESCRT-II_cplx_Vps25-sub_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13149 PTHR13149, 1 hit
PfamiView protein in Pfam
PF05871 ESCRT-II, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9BRG1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT
60 70 80 90 100
VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM
110 120 130 140 150
WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL
160 170
RALQALQQEH KAEIITVSDG RGVKFF
Length:176
Mass (Da):20,748
Last modified:June 1, 2001 - v1
Checksum:i34963A53C3DA4DD5
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EKV4K7EKV4_HUMAN
Vacuolar protein-sorting-associated...
VPS25
111Annotation score:
K7EP45K7EP45_HUMAN
Vacuolar protein-sorting-associated...
VPS25
99Annotation score:
K7ENE3K7ENE3_HUMAN
Vacuolar protein-sorting-associated...
VPS25
108Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04894076I → V. Corresponds to variant dbSNP:rs34494804Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014763 mRNA Translation: BAB87804.1
AK312092 mRNA Translation: BAG35028.1
CH471152 Genomic DNA Translation: EAW60879.1
BC006282 mRNA Translation: AAH06282.1
CCDSiCCDS11438.1
RefSeqiNP_115729.1, NM_032353.3
UniGeneiHs.500165

Genome annotation databases

EnsembliENST00000253794; ENSP00000253794; ENSG00000131475
GeneIDi84313
KEGGihsa:84313
UCSCiuc002ibi.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014763 mRNA Translation: BAB87804.1
AK312092 mRNA Translation: BAG35028.1
CH471152 Genomic DNA Translation: EAW60879.1
BC006282 mRNA Translation: AAH06282.1
CCDSiCCDS11438.1
RefSeqiNP_115729.1, NM_032353.3
UniGeneiHs.500165

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZMEX-ray2.90C/D1-102[»]
3CUQX-ray2.61C/D1-176[»]
3HTUX-ray2.00A/C/E/G102-176[»]
ProteinModelPortaliQ9BRG1
SMRiQ9BRG1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124039, 38 interactors
CORUMiQ9BRG1
IntActiQ9BRG1, 20 interactors
MINTiQ9BRG1
STRINGi9606.ENSP00000253794

PTM databases

iPTMnetiQ9BRG1
PhosphoSitePlusiQ9BRG1

Polymorphism and mutation databases

DMDMi73920459

Proteomic databases

EPDiQ9BRG1
MaxQBiQ9BRG1
PaxDbiQ9BRG1
PeptideAtlasiQ9BRG1
PRIDEiQ9BRG1
ProteomicsDBi78762

Protocols and materials databases

DNASUi84313
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253794; ENSP00000253794; ENSG00000131475
GeneIDi84313
KEGGihsa:84313
UCSCiuc002ibi.5 human

Organism-specific databases

CTDi84313
EuPathDBiHostDB:ENSG00000131475.6
GeneCardsiVPS25
HGNCiHGNC:28122 VPS25
HPAiCAB004978
HPA052217
HPA057284
MIMi610907 gene
neXtProtiNX_Q9BRG1
OpenTargetsiENSG00000131475
PharmGKBiPA142670614
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4068 Eukaryota
ENOG4111F6Y LUCA
GeneTreeiENSGT00390000014892
HOGENOMiHOG000191978
HOVERGENiHBG080015
InParanoidiQ9BRG1
KOiK12189
OMAiPPFFTIQ
OrthoDBiEOG091G0PIU
PhylomeDBiQ9BRG1
TreeFamiTF317731

Enzyme and pathway databases

ReactomeiR-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRSiVPS25 human
EvolutionaryTraceiQ9BRG1
GeneWikiiVPS25
GenomeRNAii84313
PROiPR:Q9BRG1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131475 Expressed in 193 organ(s), highest expression level in cortex of kidney
CleanExiHS_VPS25
ExpressionAtlasiQ9BRG1 baseline and differential
GenevisibleiQ9BRG1 HS

Family and domain databases

Gene3Di1.10.10.10, 1 hit
1.10.10.570, 1 hit
InterProiView protein in InterPro
IPR008570 ESCRT-II_cplx_Vps25-sub
IPR014041 ESCRT-II_cplx_Vps25-sub_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13149 PTHR13149, 1 hit
PfamiView protein in Pfam
PF05871 ESCRT-II, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiVPS25_HUMAN
AccessioniPrimary (citable) accession number: Q9BRG1
Secondary accession number(s): B2R581
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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