UniProtKB - Q9BRG1 (VPS25_HUMAN)
Vacuolar protein-sorting-associated protein 25
VPS25
Functioni
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- protein N-terminus binding Source: UniProtKB
- structural molecule activity Source: GO_Central
GO - Biological processi
- macroautophagy Source: ParkinsonsUK-UCL
- multivesicular body assembly Source: ParkinsonsUK-UCL
- negative regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
- protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
Keywordsi
Biological process | Protein transport, Transcription, Transcription regulation, Transport |
Enzyme and pathway databases
PathwayCommonsi | Q9BRG1 |
Reactomei | R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events |
SignaLinki | Q9BRG1 |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein-sorting-associated protein 25Short name: hVps25 Alternative name(s): Dermal papilla-derived protein 9 ELL-associated protein of 20 kDa ESCRT-II complex subunit VPS25 |
Gene namesi | Name:VPS25 Synonyms:DERP9, EAP20 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28122, VPS25 |
MIMi | 610907, gene |
neXtProti | NX_Q9BRG1 |
VEuPathDBi | HostDB:ENSG00000131475 |
Subcellular locationi
Nucleus
Endosome
Cytoplasm and Cytosol
Note: Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.
Cytosol
- cytosol Source: UniProtKB
Endosome
- endosome membrane Source: UniProtKB
- ESCRT II complex Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endosome, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 124 | V → E: Abolishes binding to CHMP6. 1 Publication | 1 | |
Mutagenesisi | 126 | T → K: Abolishes binding to CHMP6. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 84313 |
OpenTargetsi | ENSG00000131475 |
PharmGKBi | PA142670614 |
Miscellaneous databases
Pharosi | Q9BRG1, Tbio |
Genetic variation databases
BioMutai | VPS25 |
DMDMi | 73920459 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000215216 | 1 – 176 | Vacuolar protein-sorting-associated protein 25Add BLAST | 176 |
Proteomic databases
EPDi | Q9BRG1 |
jPOSTi | Q9BRG1 |
MassIVEi | Q9BRG1 |
MaxQBi | Q9BRG1 |
PaxDbi | Q9BRG1 |
PeptideAtlasi | Q9BRG1 |
PRIDEi | Q9BRG1 |
ProteomicsDBi | 78762 |
PTM databases
iPTMneti | Q9BRG1 |
PhosphoSitePlusi | Q9BRG1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000131475, Expressed in muscle tissue and 209 other tissues |
ExpressionAtlasi | Q9BRG1, baseline and differential |
Genevisiblei | Q9BRG1, HS |
Organism-specific databases
HPAi | ENSG00000131475, Low tissue specificity |
Interactioni
Subunit structurei
Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity).
Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25.
Interacts with CFTR; the interaction requires misfolded CFTR.
Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half).
By similarity7 PublicationsBinary interactionsi
Q9BRG1
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- protein N-terminus binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 124039, 57 interactors |
ComplexPortali | CPX-2506, ESCRT-II complex |
CORUMi | Q9BRG1 |
IntActi | Q9BRG1, 23 interactors |
MINTi | Q9BRG1 |
STRINGi | 9606.ENSP00000253794 |
Miscellaneous databases
RNActi | Q9BRG1, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9BRG1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9BRG1 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4068, Eukaryota |
GeneTreei | ENSGT00390000014892 |
HOGENOMi | CLU_087657_0_1_1 |
InParanoidi | Q9BRG1 |
OMAi | SQEFHGM |
OrthoDBi | 1094121at2759 |
PhylomeDBi | Q9BRG1 |
TreeFami | TF317731 |
Family and domain databases
DisProti | DP01598 |
Gene3Di | 1.10.10.10, 1 hit 1.10.10.570, 1 hit |
InterProi | View protein in InterPro IPR008570, ESCRT-II_cplx_Vps25-sub IPR014041, ESCRT-II_cplx_Vps25-sub_N IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13149, PTHR13149, 1 hit |
Pfami | View protein in Pfam PF05871, ESCRT-II, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT
60 70 80 90 100
VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM
110 120 130 140 150
WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL
160 170
RALQALQQEH KAEIITVSDG RGVKFF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7ENE3 | K7ENE3_HUMAN | ESCRT-II complex subunit VPS25 | VPS25 | 108 | Annotation score: | ||
K7EKV4 | K7EKV4_HUMAN | ESCRT-II complex subunit VPS25 | VPS25 | 111 | Annotation score: | ||
K7EP45 | K7EP45_HUMAN | ESCRT-II complex subunit VPS25 | VPS25 | 99 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048940 | 76 | I → V. Corresponds to variant dbSNP:rs34494804EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014763 mRNA Translation: BAB87804.1 AK312092 mRNA Translation: BAG35028.1 CH471152 Genomic DNA Translation: EAW60879.1 BC006282 mRNA Translation: AAH06282.1 |
CCDSi | CCDS11438.1 |
RefSeqi | NP_115729.1, NM_032353.3 |
Genome annotation databases
Ensembli | ENST00000253794; ENSP00000253794; ENSG00000131475 |
GeneIDi | 84313 |
KEGGi | hsa:84313 |
MANE-Selecti | ENST00000253794.7; ENSP00000253794.1; NM_032353.4; NP_115729.1 |
UCSCi | uc002ibi.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014763 mRNA Translation: BAB87804.1 AK312092 mRNA Translation: BAG35028.1 CH471152 Genomic DNA Translation: EAW60879.1 BC006282 mRNA Translation: AAH06282.1 |
CCDSi | CCDS11438.1 |
RefSeqi | NP_115729.1, NM_032353.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZME | X-ray | 2.90 | C/D | 1-102 | [»] | |
3CUQ | X-ray | 2.61 | C/D | 1-176 | [»] | |
3HTU | X-ray | 2.00 | A/C/E/G | 102-176 | [»] | |
SMRi | Q9BRG1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124039, 57 interactors |
ComplexPortali | CPX-2506, ESCRT-II complex |
CORUMi | Q9BRG1 |
IntActi | Q9BRG1, 23 interactors |
MINTi | Q9BRG1 |
STRINGi | 9606.ENSP00000253794 |
PTM databases
iPTMneti | Q9BRG1 |
PhosphoSitePlusi | Q9BRG1 |
Genetic variation databases
BioMutai | VPS25 |
DMDMi | 73920459 |
Proteomic databases
EPDi | Q9BRG1 |
jPOSTi | Q9BRG1 |
MassIVEi | Q9BRG1 |
MaxQBi | Q9BRG1 |
PaxDbi | Q9BRG1 |
PeptideAtlasi | Q9BRG1 |
PRIDEi | Q9BRG1 |
ProteomicsDBi | 78762 |
Protocols and materials databases
Antibodypediai | 29407, 228 antibodies from 31 providers |
DNASUi | 84313 |
Genome annotation databases
Ensembli | ENST00000253794; ENSP00000253794; ENSG00000131475 |
GeneIDi | 84313 |
KEGGi | hsa:84313 |
MANE-Selecti | ENST00000253794.7; ENSP00000253794.1; NM_032353.4; NP_115729.1 |
UCSCi | uc002ibi.5, human |
Organism-specific databases
CTDi | 84313 |
DisGeNETi | 84313 |
GeneCardsi | VPS25 |
HGNCi | HGNC:28122, VPS25 |
HPAi | ENSG00000131475, Low tissue specificity |
MIMi | 610907, gene |
neXtProti | NX_Q9BRG1 |
OpenTargetsi | ENSG00000131475 |
PharmGKBi | PA142670614 |
VEuPathDBi | HostDB:ENSG00000131475 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4068, Eukaryota |
GeneTreei | ENSGT00390000014892 |
HOGENOMi | CLU_087657_0_1_1 |
InParanoidi | Q9BRG1 |
OMAi | SQEFHGM |
OrthoDBi | 1094121at2759 |
PhylomeDBi | Q9BRG1 |
TreeFami | TF317731 |
Enzyme and pathway databases
PathwayCommonsi | Q9BRG1 |
Reactomei | R-HSA-917729, Endosomal Sorting Complex Required For Transport (ESCRT) R-HSA-9610379, HCMV Late Events |
SignaLinki | Q9BRG1 |
Miscellaneous databases
BioGRID-ORCSi | 84313, 787 hits in 1052 CRISPR screens |
ChiTaRSi | VPS25, human |
EvolutionaryTracei | Q9BRG1 |
GeneWikii | VPS25 |
GenomeRNAii | 84313 |
Pharosi | Q9BRG1, Tbio |
PROi | PR:Q9BRG1 |
RNActi | Q9BRG1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000131475, Expressed in muscle tissue and 209 other tissues |
ExpressionAtlasi | Q9BRG1, baseline and differential |
Genevisiblei | Q9BRG1, HS |
Family and domain databases
DisProti | DP01598 |
Gene3Di | 1.10.10.10, 1 hit 1.10.10.570, 1 hit |
InterProi | View protein in InterPro IPR008570, ESCRT-II_cplx_Vps25-sub IPR014041, ESCRT-II_cplx_Vps25-sub_N IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR13149, PTHR13149, 1 hit |
Pfami | View protein in Pfam PF05871, ESCRT-II, 1 hit |
SUPFAMi | SSF46785, SSF46785, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS25_HUMAN | |
Accessioni | Q9BRG1Primary (citable) accession number: Q9BRG1 Secondary accession number(s): B2R581 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families