Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Golgi reassembly-stacking protein 1

Gene

GORASP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (PubMed:26363069). Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (PubMed:16489344). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed:21884936).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi18Zinc; via pros nitrogenBy similarity1
Metal bindingi20Zinc; via tele nitrogenBy similarity1
Metal bindingi103ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-HSA-204005 COPII-mediated vesicle transport
R-HSA-6807878 COPI-mediated anterograde transport

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi reassembly-stacking protein 1
Alternative name(s):
Golgi peripheral membrane protein p65
Golgi phosphoprotein 5
Short name:
GOLPH5
Golgi reassembly-stacking protein of 65 kDa
Short name:
GRASP652 Publications
Gene namesi
Name:GORASP1
Synonyms:GOLPH5, GRASP65
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000114745.13
HGNCiHGNC:16769 GORASP1
MIMi606867 gene
neXtProtiNX_Q9BQQ3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi64689
OpenTargetsiENSG00000114745
PharmGKBiPA28814

Polymorphism and mutation databases

BioMutaiGORASP1
DMDMi51316077

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000875702 – 440Golgi reassembly-stacking protein 1Add BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei216PhosphothreonineCombined sources1
Modified residuei362PhosphoserineBy similarity1
Modified residuei364PhosphoserineBy similarity1
Modified residuei373PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking.By similarity
Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis.By similarity
Myristoylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ9BQQ3
MaxQBiQ9BQQ3
PaxDbiQ9BQQ3
PeptideAtlasiQ9BQQ3
PRIDEiQ9BQQ3
ProteomicsDBi78706
78707 [Q9BQQ3-2]
78708 [Q9BQQ3-3]

PTM databases

iPTMnetiQ9BQQ3
PhosphoSitePlusiQ9BQQ3

Miscellaneous databases

PMAP-CutDBiQ9BQQ3

Expressioni

Gene expression databases

BgeeiENSG00000114745 Expressed in 218 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_GORASP1
ExpressionAtlasiQ9BQQ3 baseline and differential
GenevisibleiQ9BQQ3 HS

Organism-specific databases

HPAiHPA056283

Interactioni

Subunit structurei

Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae (PubMed:26363069). Interacts with the C-terminus of GOLGA2/GM130 under both mitotic and non-mitotic conditions (PubMed:16489344, PubMed:26363069). The interaction is critical for the correct targeting of both proteins to the cis-Golgi. Interacts with TMED2 and TMED3 (By similarity).By similarity2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi122232, 56 interactors
CORUMiQ9BQQ3
IntActiQ9BQQ3, 12 interactors
MINTiQ9BQQ3
STRINGi9606.ENSP00000313869

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BQQ3
SMRiQ9BQQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 105PDZ GRASP-type 1PROSITE-ProRule annotationAdd BLAST91
Domaini111 – 199PDZ GRASP-type 2PROSITE-ProRule annotationAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 215GRASPPROSITE-ProRule annotationAdd BLAST201
Regioni190 – 202Essential for interaction with GOLGA2/GM130By similarityAdd BLAST13

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi203 – 298Pro-richAdd BLAST96
Compositional biasi295 – 298Poly-Pro4
Compositional biasi348 – 352Poly-Ser5

Sequence similaritiesi

Belongs to the GORASP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3834 Eukaryota
COG5233 LUCA
GeneTreeiENSGT00390000008686
HOGENOMiHOG000054196
HOVERGENiHBG051826
InParanoidiQ9BQQ3
OMAiEVYNIKT
OrthoDBiEOG091G0E90
PhylomeDBiQ9BQQ3

Family and domain databases

InterProiView protein in InterPro
IPR024958 GRASP55/65_PDZ
IPR007583 GRASP55_65
IPR036034 PDZ_sf
PANTHERiPTHR12893 PTHR12893, 1 hit
PfamiView protein in Pfam
PF04495 GRASP55_65, 1 hit
SUPFAMiSSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS51865 PDZ_GRASP, 2 hits

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9BQQ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGLGVSAEQP AGGAEGFHLH GVQENSPAQQ AGLEPYFDFI ITIGHSRLNK
60 70 80 90 100
ENDTLKALLK ANVEKPVKLE VFNMKTMRVR EVEVVPSNMW GGQGLLGASV
110 120 130 140 150
RFCSFRRASE QVWHVLDVEP SSPAALAGLR PYTDYVVGSD QILQESEDFF
160 170 180 190 200
TLIESHEGKP LKLMVYNSKS DSCREVTVTP NAAWGGEGSL GCGIGYGYLH
210 220 230 240 250
RIPTQPPSYH KKPPGTPPPS ALPLGAPPPD ALPPGPTPED SPSLETGSRQ
260 270 280 290 300
SDYMEALLQA PGSSMEDPLP GPGSPSHSAP DPDGLPHFME TPLQPPPPVQ
310 320 330 340 350
RVMDPGFLDV SGISLLDNSN ASVWPSLPSS TELTTTAVST SGPEDICSSS
360 370 380 390 400
SSHERGGEAT WSGSEFEVSF LDSPGAQAQA DHLPQLTLPD SLTSAASPED
410 420 430 440
GLSAELLEAQ AEEEPASTEG LDTGTEAEGL DSQAQISTTE
Length:440
Mass (Da):46,482
Last modified:January 23, 2007 - v3
Checksum:i1187C0356E16CE00
GO
Isoform 2 (identifier: Q9BQQ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-440: VTVTPNAAWG...DSQAQISTTE → SGMWHWLWVS...FCSTTWCPTT

Note: No experimental confirmation available.
Show »
Length:215
Mass (Da):23,906
Checksum:i0EC77420BA924801
GO
Isoform 3 (identifier: Q9BQQ3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
     306-319: GFLDVSGISLLDNS → AMPVCGPACPLPQN
     320-440: Missing.

Note: No experimental confirmation available.
Show »
Length:246
Mass (Da):26,237
Checksum:i9EC9AC172F80403D
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4E1H8B4E1H8_HUMAN
cDNA FLJ58222, highly similar to Go...
GORASP1
345Annotation score:
B3KPY8B3KPY8_HUMAN
Golgi reassembly stacking protein 1...
GORASP1 hCG_19028
285Annotation score:
C9J0V5C9J0V5_HUMAN
Golgi reassembly-stacking protein 1
GORASP1
193Annotation score:
C9J5S6C9J5S6_HUMAN
Golgi reassembly-stacking protein 1
GORASP1
69Annotation score:
H7C0J2H7C0J2_HUMAN
Golgi reassembly-stacking protein 1
GORASP1
141Annotation score:
G3V0G1G3V0G1_HUMAN
Golgi reassembly stacking protein 1...
GORASP1 hCG_19028
316Annotation score:
C9J9V9C9J9V9_HUMAN
Golgi reassembly-stacking protein 1
GORASP1
88Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271G → E in BAC03598 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051016425T → M. Corresponds to variant dbSNP:rs1109643Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0113061 – 73Missing in isoform 3. 1 PublicationAdd BLAST73
Alternative sequenceiVSP_011307176 – 440VTVTP…ISTTE → SGMWHWLWVSTPDPNSAPQL PQEATWHPTTFCSTTWCPTT in isoform 2. 1 PublicationAdd BLAST265
Alternative sequenceiVSP_011308306 – 319GFLDV…LLDNS → AMPVCGPACPLPQN in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_011309320 – 440Missing in isoform 3. 1 PublicationAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ409349 mRNA Translation: CAC35160.1
AK091168 mRNA Translation: BAC03598.1
AK124755 mRNA Translation: BAG54090.1
CH471055 Genomic DNA Translation: EAW64554.1
BC008928 mRNA Translation: AAH08928.1
BC075854 mRNA Translation: AAH75854.1
BC103826 mRNA Translation: AAI03827.1
BC103827 mRNA Translation: AAI03828.1
CCDSiCCDS2681.1 [Q9BQQ3-1]
RefSeqiNP_001265718.1, NM_001278789.1
NP_001265719.1, NM_001278790.1
NP_114105.1, NM_031899.3 [Q9BQQ3-1]
UniGeneiHs.721946

Genome annotation databases

EnsembliENST00000319283; ENSP00000313869; ENSG00000114745 [Q9BQQ3-1]
ENST00000431601; ENSP00000403552; ENSG00000114745 [Q9BQQ3-3]
ENST00000452389; ENSP00000403167; ENSG00000114745 [Q9BQQ3-2]
GeneIDi64689
KEGGihsa:64689
UCSCiuc003ciw.3 human [Q9BQQ3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ409349 mRNA Translation: CAC35160.1
AK091168 mRNA Translation: BAC03598.1
AK124755 mRNA Translation: BAG54090.1
CH471055 Genomic DNA Translation: EAW64554.1
BC008928 mRNA Translation: AAH08928.1
BC075854 mRNA Translation: AAH75854.1
BC103826 mRNA Translation: AAI03827.1
BC103827 mRNA Translation: AAI03828.1
CCDSiCCDS2681.1 [Q9BQQ3-1]
RefSeqiNP_001265718.1, NM_001278789.1
NP_001265719.1, NM_001278790.1
NP_114105.1, NM_031899.3 [Q9BQQ3-1]
UniGeneiHs.721946

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4REYX-ray1.96A2-210[»]
ProteinModelPortaliQ9BQQ3
SMRiQ9BQQ3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122232, 56 interactors
CORUMiQ9BQQ3
IntActiQ9BQQ3, 12 interactors
MINTiQ9BQQ3
STRINGi9606.ENSP00000313869

PTM databases

iPTMnetiQ9BQQ3
PhosphoSitePlusiQ9BQQ3

Polymorphism and mutation databases

BioMutaiGORASP1
DMDMi51316077

Proteomic databases

EPDiQ9BQQ3
MaxQBiQ9BQQ3
PaxDbiQ9BQQ3
PeptideAtlasiQ9BQQ3
PRIDEiQ9BQQ3
ProteomicsDBi78706
78707 [Q9BQQ3-2]
78708 [Q9BQQ3-3]

Protocols and materials databases

DNASUi64689
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319283; ENSP00000313869; ENSG00000114745 [Q9BQQ3-1]
ENST00000431601; ENSP00000403552; ENSG00000114745 [Q9BQQ3-3]
ENST00000452389; ENSP00000403167; ENSG00000114745 [Q9BQQ3-2]
GeneIDi64689
KEGGihsa:64689
UCSCiuc003ciw.3 human [Q9BQQ3-1]

Organism-specific databases

CTDi64689
DisGeNETi64689
EuPathDBiHostDB:ENSG00000114745.13
GeneCardsiGORASP1
HGNCiHGNC:16769 GORASP1
HPAiHPA056283
MIMi606867 gene
neXtProtiNX_Q9BQQ3
OpenTargetsiENSG00000114745
PharmGKBiPA28814
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3834 Eukaryota
COG5233 LUCA
GeneTreeiENSGT00390000008686
HOGENOMiHOG000054196
HOVERGENiHBG051826
InParanoidiQ9BQQ3
OMAiEVYNIKT
OrthoDBiEOG091G0E90
PhylomeDBiQ9BQQ3

Enzyme and pathway databases

ReactomeiR-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-HSA-204005 COPII-mediated vesicle transport
R-HSA-6807878 COPI-mediated anterograde transport

Miscellaneous databases

ChiTaRSiGORASP1 human
GeneWikiiGORASP1
GenomeRNAii64689
PMAP-CutDBiQ9BQQ3
PROiPR:Q9BQQ3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114745 Expressed in 218 organ(s), highest expression level in left lobe of thyroid gland
CleanExiHS_GORASP1
ExpressionAtlasiQ9BQQ3 baseline and differential
GenevisibleiQ9BQQ3 HS

Family and domain databases

InterProiView protein in InterPro
IPR024958 GRASP55/65_PDZ
IPR007583 GRASP55_65
IPR036034 PDZ_sf
PANTHERiPTHR12893 PTHR12893, 1 hit
PfamiView protein in Pfam
PF04495 GRASP55_65, 1 hit
SUPFAMiSSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS51865 PDZ_GRASP, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGORS1_HUMAN
AccessioniPrimary (citable) accession number: Q9BQQ3
Secondary accession number(s): B3KWC8
, Q3SYG7, Q8N272, Q96H42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again