Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxisomal NADH pyrophosphatase NUDT12

Gene

NUDT12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes NAD(P)H to NMNH and AMP (2',5'-ADP), and diadenosine diphosphate to AMP. Has also activity towards NAD(P)+, ADP-ribose and diadenosine triphosphate. May act to regulate the concentration of peroxisomal nicotinamide nucleotide cofactors required for oxidative metabolism in this organelle.1 Publication

Catalytic activityi

NAD(H) + H2O = AMP + NMN(H).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Kineticsi

  1. KM=11 µM for NADH1 Publication
  2. KM=16 µM for NADPH1 Publication
  3. KM=190 µM for NAD1 Publication

    pH dependencei

    Optimum pH is 8-9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi370Magnesium or manganeseBy similarity1
    Metal bindingi374Magnesium or manganeseBy similarity1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • NAD+ diphosphatase activity Source: UniProtKB
    • NADH pyrophosphatase activity Source: UniProtKB

    GO - Biological processi

    • NAD biosynthesis via nicotinamide riboside salvage pathway Source: Reactome
    • NAD catabolic process Source: UniProtKB
    • NADP catabolic process Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Manganese, Metal-binding, NAD, NADP

    Enzyme and pathway databases

    ReactomeiR-HSA-197264 Nicotinamide salvaging

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal NADH pyrophosphatase NUDT12 (EC:3.6.1.22)
    Alternative name(s):
    Nucleoside diphosphate-linked moiety X motif 12
    Short name:
    Nudix motif 12
    Gene namesi
    Name:NUDT12
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 5

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000112874.9
    HGNCiHGNC:18826 NUDT12
    MIMi609232 gene
    neXtProtiNX_Q9BQG2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Peroxisome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi460 – 462Missing : Abolishes localization to peroxisomes. 1 Publication3

    Organism-specific databases

    DisGeNETi83594
    OpenTargetsiENSG00000112874
    PharmGKBiPA38699

    Polymorphism and mutation databases

    BioMutaiNUDT12
    DMDMi68565930

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000569561 – 462Peroxisomal NADH pyrophosphatase NUDT12Add BLAST462

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei185N6-succinyllysineBy similarity1
    Modified residuei292N6-succinyllysineBy similarity1

    Proteomic databases

    EPDiQ9BQG2
    MaxQBiQ9BQG2
    PaxDbiQ9BQG2
    PeptideAtlasiQ9BQG2
    PRIDEiQ9BQG2
    ProteomicsDBi78677

    PTM databases

    iPTMnetiQ9BQG2
    PhosphoSitePlusiQ9BQG2

    Expressioni

    Gene expression databases

    BgeeiENSG00000112874
    CleanExiHS_NUDT12
    ExpressionAtlasiQ9BQG2 baseline and differential
    GenevisibleiQ9BQG2 HS

    Organism-specific databases

    HPAiHPA045449

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BLMHQ138676EBI-10230612,EBI-718504

    Protein-protein interaction databases

    BioGridi123690, 18 interactors
    IntActiQ9BQG2, 2 interactors
    STRINGi9606.ENSP00000230792

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BQG2
    SMRiQ9BQG2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati11 – 40ANK 1Add BLAST30
    Repeati45 – 74ANK 2Add BLAST30
    Repeati78 – 98ANK 3Add BLAST21
    Domaini319 – 453Nudix hydrolasePROSITE-ProRule annotationAdd BLAST135

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi355 – 376Nudix boxAdd BLAST22
    Motifi460 – 462Microbody targeting signalCurated3

    Sequence similaritiesi

    Belongs to the Nudix hydrolase family. NudC subfamily.Curated

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiKOG0504 Eukaryota
    KOG3084 Eukaryota
    COG2816 LUCA
    GeneTreeiENSGT00530000063600
    HOGENOMiHOG000247937
    HOVERGENiHBG054394
    InParanoidiQ9BQG2
    KOiK03426
    OMAiQPEVRLC
    OrthoDBiEOG091G0SLC
    PhylomeDBiQ9BQG2
    TreeFamiTF106352

    Family and domain databases

    CDDicd00204 ANK, 1 hit
    Gene3Di1.25.40.20, 1 hit
    InterProiView protein in InterPro
    IPR002110 Ankyrin_rpt
    IPR020683 Ankyrin_rpt-contain_dom
    IPR036770 Ankyrin_rpt-contain_sf
    IPR015375 NADH_PPase-like_N
    IPR015797 NUDIX_hydrolase-like_dom_sf
    IPR020084 NUDIX_hydrolase_CS
    IPR000086 NUDIX_hydrolase_dom
    IPR015376 Znr_NADH_PPase
    PfamiView protein in Pfam
    PF12796 Ank_2, 1 hit
    PF00293 NUDIX, 1 hit
    PF09296 NUDIX-like, 1 hit
    PF09297 zf-NADH-PPase, 1 hit
    SMARTiView protein in SMART
    SM00248 ANK, 3 hits
    SUPFAMiSSF48403 SSF48403, 1 hit
    SSF55811 SSF55811, 1 hit
    PROSITEiView protein in PROSITE
    PS50297 ANK_REP_REGION, 1 hit
    PS50088 ANK_REPEAT, 1 hit
    PS51462 NUDIX, 1 hit
    PS00893 NUDIX_BOX, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9BQG2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSVKRSLKQ EIVTQFHCSA AEGDIAKLTG ILSHSPSLLN ETSENGWTAL
    60 70 80 90 100
    MYAARNGHPE IVQFLLEKGC DRSIVNKSRQ TALDIAVFWG YKHIANLLAT
    110 120 130 140 150
    AKGGKKPWFL TNEVEECENY FSKTLLDRKS EKRNNSDWLL AKESHPATVF
    160 170 180 190 200
    ILFSDLNPLV TLGGNKESFQ QPEVRLCQLN YTDIKDYLAQ PEKITLIFLG
    210 220 230 240 250
    VELEIKDKLL NYAGEVPREE EDGLVAWFAL GIDPIAAEEF KQRHENCYFL
    260 270 280 290 300
    HPPMPALLQL KEKEAGVVAQ ARSVLAWHSR YKFCPTCGNA TKIEEGGYKR
    310 320 330 340 350
    LCLKEDCPSL NGVHNTSYPR VDPVVIMQVI HPDGTKCLLG RQKRFPPGMF
    360 370 380 390 400
    TCLAGFIEPG ETIEDAVRRE VEEESGVKVG HVQYVACQPW PMPSSLMIGC
    410 420 430 440 450
    LALAVSTEIK VDKNEIEDAR WFTREQVLDV LTKGKQQAFF VPPSRAIAHQ
    460
    LIKHWIRINP NL
    Length:462
    Mass (Da):52,076
    Last modified:June 1, 2001 - v1
    Checksum:i1A470B93707DB1D5
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_034157129K → E. Corresponds to variant dbSNP:rs35903418Ensembl.1
    Natural variantiVAR_034158235I → V. Corresponds to variant dbSNP:rs34468716Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL136592 mRNA Translation: CAB66527.1
    AK098066 mRNA Translation: BAG53574.1
    CH471086 Genomic DNA Translation: EAW49073.1
    BC026748 mRNA Translation: AAH26748.1
    BC041099 mRNA Translation: AAH41099.1
    CCDSiCCDS4096.1
    RefSeqiNP_001287670.1, NM_001300741.1
    NP_113626.1, NM_031438.3
    XP_005272152.1, XM_005272095.1
    UniGeneiHs.434289

    Genome annotation databases

    EnsembliENST00000230792; ENSP00000230792; ENSG00000112874
    GeneIDi83594
    KEGGihsa:83594
    UCSCiuc003koi.4 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiNUD12_HUMAN
    AccessioniPrimary (citable) accession number: Q9BQG2
    Secondary accession number(s): B3KUW2, Q8TAL7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: June 1, 2001
    Last modified: June 20, 2018
    This is version 148 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health