UniProtKB - Q9BQ83 (SLX1_HUMAN)
Functioni
Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products.
UniRule annotation3 PublicationsCaution
Cofactori
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 186 – 238 | SLX1-typeUniRule annotationAdd BLAST | 53 |
GO - Molecular functioni
- 5'-flap endonuclease activity Source: UniProtKB
- crossover junction endodeoxyribonuclease activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA double-strand break processing involved in repair via single-strand annealing Source: UniProtKB
- DNA repair Source: UniProtKB
- double-strand break repair via homologous recombination Source: UniProtKB
- negative regulation of telomere maintenance via telomere lengthening Source: BHF-UCL
- positive regulation of t-circle formation Source: BHF-UCL
- t-circle formation Source: BHF-UCL
- telomere maintenance via telomere lengthening Source: BHF-UCL
- telomeric D-loop disassembly Source: BHF-UCL
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA recombination, DNA repair |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9BQ83 |
Reactomei | R-HSA-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates R-HSA-6783310, Fanconi Anemia Pathway |
SignaLinki | Q9BQ83 |
Names & Taxonomyi
Protein namesi | Recommended name: Structure-specific endonuclease subunit SLX1UniRule annotation (EC:3.1.-.-UniRule annotation)Alternative name(s): GIY-YIG domain-containing protein 1UniRule annotation |
Gene namesi | AND |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20922, SLX1A HGNC:28748, SLX1B |
MIMi | 615822, gene 615823, gene |
neXtProti | NX_Q9BQ83 |
VEuPathDBi | HostDB:ENSG00000132207 HostDB:ENSG00000181625 |
Subcellular locationi
Nucleus
- Nucleus UniRule annotation1 Publication
Nucleus
- nucleoplasm Source: HPA
- Slx1-Slx4 complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 41 | R → A: Abolishes endonucleolytic activity. 1 Publication | 1 | |
Mutagenesisi | 82 | E → A: Abolishes endonucleolytic activity. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 548593 79008 |
OpenTargetsi | ENSG00000132207 |
PharmGKBi | PA142671738 |
Miscellaneous databases
Pharosi | Q9BQ83, Tbio |
Genetic variation databases
BioMutai | SLX1A |
DMDMi | 74732820 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000332120 | 1 – 275 | Structure-specific endonuclease subunit SLX1Add BLAST | 275 |
Proteomic databases
MassIVEi | Q9BQ83 |
PaxDbi | Q9BQ83 |
PeptideAtlasi | Q9BQ83 |
PRIDEi | Q9BQ83 |
PTM databases
iPTMneti | Q9BQ83 |
PhosphoSitePlusi | Q9BQ83 |
Expressioni
Gene expression databases
Bgeei | ENSG00000132207, Expressed in testis and 111 other tissues |
ExpressionAtlasi | Q9BQ83, baseline and differential |
Genevisiblei | Q9BQ83, HS |
Organism-specific databases
HPAi | ENSG00000132207, Low tissue specificity ENSG00000181625, Low tissue specificity |
Interactioni
Subunit structurei
Forms a heterodimer with SLX4.
Binary interactionsi
Q9BQ83
With | #Exp. | IntAct |
---|---|---|
SLX4 [Q8IY92] | 10 | EBI-2370858,EBI-2370740 |
Protein-protein interaction databases
BioGRIDi | 122479, 45 interactors 139225, 26 interactors |
IntActi | Q9BQ83, 70 interactors |
STRINGi | 9606.ENSP00000251303 |
Miscellaneous databases
RNActi | Q9BQ83, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 95 | GIY-YIGUniRule annotationAdd BLAST | 84 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 148 – 179 | DisorderedSequence analysisAdd BLAST | 32 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 148 – 164 | Pro residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 186 – 238 | SLX1-typeUniRule annotationAdd BLAST | 53 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3005, Eukaryota |
GeneTreei | ENSGT00390000013368 |
HOGENOMi | CLU_030739_0_0_1 |
InParanoidi | Q9BQ83 |
OMAi | LQFEHAW |
OrthoDBi | 844266at2759 |
PhylomeDBi | Q9BQ83 |
TreeFami | TF352344 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit 3.40.1440.10, 1 hit |
HAMAPi | MF_03100, Endonuc_su_Slx1, 1 hit |
InterProi | View protein in InterPro IPR000305, GIY-YIG_endonuc IPR035901, GIY-YIG_endonuc_sf IPR027520, Slx1 IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01541, GIY-YIG, 1 hit |
SUPFAMi | SSF82771, SSF82771, 1 hit |
PROSITEi | View protein in PROSITE PS50164, GIY_YIG, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGPAGVAARP GRFFGVYLLY CLNPRYRGRV YVGFTVNTAR RVQQHNGGRK
60 70 80 90 100
KGGAWRTSGR GPWEMVLVVH GFPSSVAALR FEWAWQHPHA SRRLAHVGPR
110 120 130 140 150
LRGETAFAFH LRVLAHMLRA PPWARLPLTL RWVRPDLRQD LCLPPPPHVP
160 170 180 190 200
LAFGPPPPQA PAPRRRAGPF DDAEPEPDQG DPGACCSLCA QTIQDEEGPL
210 220 230 240 250
CCPHPGCLLR AHVICLAEEF LQEEPGQLLP LEGQCPCCEK SLLWGDLIWL
260 270
CQMDTEKEVE DSELEEAHWT DLLET
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BPC3 | H3BPC3_HUMAN | Structure-specific endonuclease sub... | SLX1A | 81 | Annotation score: | ||
H3BRR6 | H3BRR6_HUMAN | Structure-specific endonuclease sub... | SLX1B | 88 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_033331 | 81 – 194 | Missing in isoform 2. 1 PublicationAdd BLAST | 114 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC106782 Genomic DNA No translation available. AC133555 Genomic DNA No translation available. BC000754 mRNA Translation: AAH00754.1 BC000803 mRNA Translation: AAH00803.1 BC015990 mRNA Translation: AAH15990.1 BC019306 mRNA Translation: AAH19306.1 BC069007 mRNA Translation: AAH69007.1 BC130545 mRNA Translation: AAI30546.1 BC130547 mRNA Translation: AAI30548.1 BC144462 mRNA Translation: AAI44463.1 |
CCDSi | CCDS10648.1 [Q9BQ83-1] CCDS10649.1 [Q9BQ83-2] CCDS32431.1 [Q9BQ83-1] CCDS32432.1 [Q9BQ83-2] |
RefSeqi | NP_001014999.1, NM_001014999.2 [Q9BQ83-1] NP_001015000.1, NM_001015000.2 [Q9BQ83-2] NP_076949.1, NM_024044.3 [Q9BQ83-1] NP_835145.1, NM_178044.2 [Q9BQ83-2] |
Genome annotation databases
Ensembli | ENST00000251303; ENSP00000251303; ENSG00000132207 ENST00000330181; ENSP00000328940; ENSG00000181625 ENST00000345535; ENSP00000333945; ENSG00000132207 [Q9BQ83-2] ENST00000351581; ENSP00000335316; ENSG00000181625 [Q9BQ83-2] |
GeneIDi | 548593 79008 |
KEGGi | hsa:548593 hsa:79008 |
MANE-Selecti | ENST00000251303.11; ENSP00000251303.7; NM_001014999.3; NP_001014999.1 ENST00000330181.9; ENSP00000328940.5; NM_024044.4; NP_076949.1 |
UCSCi | uc002dsx.4, human [Q9BQ83-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC106782 Genomic DNA No translation available. AC133555 Genomic DNA No translation available. BC000754 mRNA Translation: AAH00754.1 BC000803 mRNA Translation: AAH00803.1 BC015990 mRNA Translation: AAH15990.1 BC019306 mRNA Translation: AAH19306.1 BC069007 mRNA Translation: AAH69007.1 BC130545 mRNA Translation: AAI30546.1 BC130547 mRNA Translation: AAI30548.1 BC144462 mRNA Translation: AAI44463.1 |
CCDSi | CCDS10648.1 [Q9BQ83-1] CCDS10649.1 [Q9BQ83-2] CCDS32431.1 [Q9BQ83-1] CCDS32432.1 [Q9BQ83-2] |
RefSeqi | NP_001014999.1, NM_001014999.2 [Q9BQ83-1] NP_001015000.1, NM_001015000.2 [Q9BQ83-2] NP_076949.1, NM_024044.3 [Q9BQ83-1] NP_835145.1, NM_178044.2 [Q9BQ83-2] |
3D structure databases
SMRi | Q9BQ83 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122479, 45 interactors 139225, 26 interactors |
IntActi | Q9BQ83, 70 interactors |
STRINGi | 9606.ENSP00000251303 |
PTM databases
iPTMneti | Q9BQ83 |
PhosphoSitePlusi | Q9BQ83 |
Genetic variation databases
BioMutai | SLX1A |
DMDMi | 74732820 |
Proteomic databases
MassIVEi | Q9BQ83 |
PaxDbi | Q9BQ83 |
PeptideAtlasi | Q9BQ83 |
PRIDEi | Q9BQ83 |
Protocols and materials databases
Antibodypediai | 26716, 58 antibodies from 16 providers 63854, 7 antibodies from 5 providers |
DNASUi | 79008 |
Genome annotation databases
Ensembli | ENST00000251303; ENSP00000251303; ENSG00000132207 ENST00000330181; ENSP00000328940; ENSG00000181625 ENST00000345535; ENSP00000333945; ENSG00000132207 [Q9BQ83-2] ENST00000351581; ENSP00000335316; ENSG00000181625 [Q9BQ83-2] |
GeneIDi | 548593 79008 |
KEGGi | hsa:548593 hsa:79008 |
MANE-Selecti | ENST00000251303.11; ENSP00000251303.7; NM_001014999.3; NP_001014999.1 ENST00000330181.9; ENSP00000328940.5; NM_024044.4; NP_076949.1 |
UCSCi | uc002dsx.4, human [Q9BQ83-1] |
Organism-specific databases
CTDi | 548593 79008 |
DisGeNETi | 548593 79008 |
GeneCardsi | SLX1A SLX1B |
HGNCi | HGNC:20922, SLX1A HGNC:28748, SLX1B |
HPAi | ENSG00000132207, Low tissue specificity ENSG00000181625, Low tissue specificity |
MIMi | 615822, gene 615823, gene |
neXtProti | NX_Q9BQ83 |
OpenTargetsi | ENSG00000132207 |
PharmGKBi | PA142671738 |
VEuPathDBi | HostDB:ENSG00000132207 HostDB:ENSG00000181625 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3005, Eukaryota |
GeneTreei | ENSGT00390000013368 |
HOGENOMi | CLU_030739_0_0_1 |
InParanoidi | Q9BQ83 |
OMAi | LQFEHAW |
OrthoDBi | 844266at2759 |
PhylomeDBi | Q9BQ83 |
TreeFami | TF352344 |
Enzyme and pathway databases
PathwayCommonsi | Q9BQ83 |
Reactomei | R-HSA-5693568, Resolution of D-loop Structures through Holliday Junction Intermediates R-HSA-6783310, Fanconi Anemia Pathway |
SignaLinki | Q9BQ83 |
Miscellaneous databases
BioGRID-ORCSi | 548593, 26 hits in 573 CRISPR screens 79008, 12 hits in 194 CRISPR screens |
ChiTaRSi | SLX1A, human |
Pharosi | Q9BQ83, Tbio |
PROi | PR:Q9BQ83 |
RNActi | Q9BQ83, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132207, Expressed in testis and 111 other tissues |
ExpressionAtlasi | Q9BQ83, baseline and differential |
Genevisiblei | Q9BQ83, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit 3.40.1440.10, 1 hit |
HAMAPi | MF_03100, Endonuc_su_Slx1, 1 hit |
InterProi | View protein in InterPro IPR000305, GIY-YIG_endonuc IPR035901, GIY-YIG_endonuc_sf IPR027520, Slx1 IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01541, GIY-YIG, 1 hit |
SUPFAMi | SSF82771, SSF82771, 1 hit |
PROSITEi | View protein in PROSITE PS50164, GIY_YIG, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SLX1_HUMAN | |
Accessioni | Q9BQ83Primary (citable) accession number: Q9BQ83 Secondary accession number(s): B7ZME1, Q6NTG6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families