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Protein

Three prime repair exonuclease 2

Gene

TREX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exonuclease with a preference for double-stranded DNA with mismatched 3' termini. May play a role in DNA repair.1 Publication

Caution

The gene for this protein is either identical to or adjacent to that of UCHL5IP. Most mRNAs that encode UCHL5IP also include the N-terminal part of TREX2.Curated

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ per subunit. The second magnesium ion interacts with only one residue. Substitution with Mn2+ results in partial activity.By similarity

pH dependencei

Optimum pH is 7.5-8.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57Magnesium 1By similarity1
Metal bindingi57Magnesium 2By similarity1
Metal bindingi59Magnesium 1By similarity1
Binding sitei165SubstrateBy similarity1
Active sitei231Proton donor/acceptorBy similarity1
Metal bindingi236Magnesium 1By similarity1
Binding sitei236SubstrateBy similarity1

GO - Molecular functioni

  • 3'-5'-exodeoxyribonuclease activity Source: UniProtKB
  • exodeoxyribonuclease III activity Source: UniProtKB-EC
  • magnesium ion binding Source: UniProtKB
  • nucleic acid binding Source: InterPro
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • DNA metabolic process Source: UniProtKB
  • DNA repair Source: ProtInc

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Three prime repair exonuclease 2 (EC:3.1.11.2)
Alternative name(s):
3'-5' exonuclease TREX2
Gene namesi
Name:TREX2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000183479.12
HGNCiHGNC:12270 TREX2
MIMi300370 gene
neXtProtiNX_Q9BQ50

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57D → A: Loss of enzyme activity; when associated with A-59. Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi59E → A: Loss of enzyme activity; when associated with A-57. Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi166D → A: Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi206R → A: Strongly reduces DNA-binding; when associated with A-208 and A-210. 1 Publication1
Mutagenesisi208R → A: Strongly reduces DNA-binding; when associated with A-206 and A-210. 1 Publication1
Mutagenesisi210R → A: Strongly reduces DNA-binding; when associated with A-208 and A-208. 1 Publication1
Mutagenesisi231H → A: Loss of enzyme activity; when associated with A-236. Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi236D → A: Loss of enzyme activity; when associated with A-231. Almost abolishes enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi11219
OpenTargetsiENSG00000183479
PharmGKBiPA36950

Polymorphism and mutation databases

BioMutaiTREX2
DMDMi47606206

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001098701 – 279Three prime repair exonuclease 2Add BLAST279

Proteomic databases

EPDiQ9BQ50
PaxDbiQ9BQ50
PeptideAtlasiQ9BQ50
PRIDEiQ9BQ50
ProteomicsDBi78622
78623 [Q9BQ50-2]

PTM databases

PhosphoSitePlusiQ9BQ50

Expressioni

Tissue specificityi

Detected in heart, breast, prostate, skeletal muscle, testis, uterus, bone marrow, colon, small intestine, stomach and thymus.1 Publication

Gene expression databases

BgeeiENSG00000183479 Expressed in 80 organ(s), highest expression level in skin of leg
CleanExiHS_TREX2
GenevisibleiQ9BQ50 HS

Organism-specific databases

HPAiHPA054060

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116388, 12 interactors
IntActiQ9BQ50, 2 interactors
STRINGi9606.ENSP00000333441

Structurei

Secondary structure

1279
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9BQ50
SMRiQ9BQ50
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BQ50

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 60Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the exonuclease superfamily. TREX family.Curated

Phylogenomic databases

eggNOGiKOG4793 Eukaryota
ENOG4111YSP LUCA
GeneTreeiENSGT00390000012715
HOGENOMiHOG000118119
HOVERGENiHBG079278
InParanoidiQ9BQ50
KOiK10791
OMAiDVHTLLM
OrthoDBiEOG091G0LSM
PhylomeDBiQ9BQ50
TreeFamiTF323333

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR034926 TREX2
PANTHERiPTHR13058:SF20 PTHR13058:SF20, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9BQ50-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGRAGSPLPR SSWPRMDDCG SRSRCSPTLC SSLRTCYPRG NITMSEAPRA
60 70 80 90 100
ETFVFLDLEA TGLPSVEPEI AELSLFAVHR SSLENPEHDE SGALVLPRVL
110 120 130 140 150
DKLTLCMCPE RPFTAKASEI TGLSSEGLAR CRKAGFDGAV VRTLQAFLSR
160 170 180 190 200
QAGPICLVAH NGFDYDFPLL CAELRRLGAR LPRDTVCLDT LPALRGLDRA
210 220 230 240 250
HSHGTRARGR QGYSLGSLFH RYFRAEPSAA HSAEGDVHTL LLIFLHRAAE
260 270
LLAWADEQAR GWAHIEPMYL PPDDPSLEA
Length:279
Mass (Da):30,621
Last modified:June 1, 2001 - v1
Checksum:i91387E045960C1EA
GO
Isoform 2 (identifier: Q9BQ50-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Show »
Length:236
Mass (Da):25,922
Checksum:i3363BE40CFCC2300
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025211180R → C1 PublicationCorresponds to variant dbSNP:rs35132777Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0104471 – 43Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151107 mRNA Translation: AAD48776.1
AF319570 mRNA Translation: AAK07617.1
AF319571 mRNA Translation: AAK07618.1
AF319572 mRNA Translation: AAK07619.1
AF319573 mRNA Translation: AAK07620.1
DQ145722 Genomic DNA Translation: AAZ38719.1
CCDSiCCDS35437.1 [Q9BQ50-2]
RefSeqiNP_542432.2, NM_080701.3 [Q9BQ50-2]
UniGeneiHs.644635

Genome annotation databases

EnsembliENST00000330912; ENSP00000333441; ENSG00000183479 [Q9BQ50-2]
ENST00000334497; ENSP00000334993; ENSG00000183479 [Q9BQ50-1]
ENST00000338525; ENSP00000345218; ENSG00000183479 [Q9BQ50-2]
ENST00000370212; ENSP00000359231; ENSG00000183479 [Q9BQ50-1]
ENST00000370231; ENSP00000359251; ENSG00000183479 [Q9BQ50-2]
ENST00000370232; ENSP00000359252; ENSG00000183479 [Q9BQ50-1]
ENST00000393862; ENSP00000377442; ENSG00000183479 [Q9BQ50-2]
GeneIDi11219
KEGGihsa:11219
UCSCiuc011myp.2 human [Q9BQ50-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151107 mRNA Translation: AAD48776.1
AF319570 mRNA Translation: AAK07617.1
AF319571 mRNA Translation: AAK07618.1
AF319572 mRNA Translation: AAK07619.1
AF319573 mRNA Translation: AAK07620.1
DQ145722 Genomic DNA Translation: AAZ38719.1
CCDSiCCDS35437.1 [Q9BQ50-2]
RefSeqiNP_542432.2, NM_080701.3 [Q9BQ50-2]
UniGeneiHs.644635

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y97X-ray2.50A/B44-279[»]
ProteinModelPortaliQ9BQ50
SMRiQ9BQ50
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116388, 12 interactors
IntActiQ9BQ50, 2 interactors
STRINGi9606.ENSP00000333441

PTM databases

PhosphoSitePlusiQ9BQ50

Polymorphism and mutation databases

BioMutaiTREX2
DMDMi47606206

Proteomic databases

EPDiQ9BQ50
PaxDbiQ9BQ50
PeptideAtlasiQ9BQ50
PRIDEiQ9BQ50
ProteomicsDBi78622
78623 [Q9BQ50-2]

Protocols and materials databases

DNASUi11219
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330912; ENSP00000333441; ENSG00000183479 [Q9BQ50-2]
ENST00000334497; ENSP00000334993; ENSG00000183479 [Q9BQ50-1]
ENST00000338525; ENSP00000345218; ENSG00000183479 [Q9BQ50-2]
ENST00000370212; ENSP00000359231; ENSG00000183479 [Q9BQ50-1]
ENST00000370231; ENSP00000359251; ENSG00000183479 [Q9BQ50-2]
ENST00000370232; ENSP00000359252; ENSG00000183479 [Q9BQ50-1]
ENST00000393862; ENSP00000377442; ENSG00000183479 [Q9BQ50-2]
GeneIDi11219
KEGGihsa:11219
UCSCiuc011myp.2 human [Q9BQ50-1]

Organism-specific databases

CTDi11219
DisGeNETi11219
EuPathDBiHostDB:ENSG00000183479.12
GeneCardsiTREX2
H-InvDBiHIX0017129
HGNCiHGNC:12270 TREX2
HPAiHPA054060
MIMi300370 gene
neXtProtiNX_Q9BQ50
OpenTargetsiENSG00000183479
PharmGKBiPA36950
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4793 Eukaryota
ENOG4111YSP LUCA
GeneTreeiENSGT00390000012715
HOGENOMiHOG000118119
HOVERGENiHBG079278
InParanoidiQ9BQ50
KOiK10791
OMAiDVHTLLM
OrthoDBiEOG091G0LSM
PhylomeDBiQ9BQ50
TreeFamiTF323333

Miscellaneous databases

ChiTaRSiTREX2 human
EvolutionaryTraceiQ9BQ50
GeneWikiiTREX2
GenomeRNAii11219
PROiPR:Q9BQ50
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000183479 Expressed in 80 organ(s), highest expression level in skin of leg
CleanExiHS_TREX2
GenevisibleiQ9BQ50 HS

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR034926 TREX2
PANTHERiPTHR13058:SF20 PTHR13058:SF20, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTREX2_HUMAN
AccessioniPrimary (citable) accession number: Q9BQ50
Secondary accession number(s): Q45F08, Q9UN77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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