UniProtKB - Q9BGI2 (PRDX4_BOVIN)
Protein
Peroxiredoxin-4
Gene
PRDX4
Organism
Bos taurus (Bovine)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.By similarity
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity
Catalytic activityi
- EC:1.11.1.24By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 127 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- thioredoxin peroxidase activity Source: GO_Central
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- response to oxidative stress Source: GO_Central
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Protein family/group databases
PeroxiBasei | 4531, Bt2CysPrx04 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxiredoxin-4 (EC:1.11.1.24By similarity)Alternative name(s): Peroxiredoxin IV Short name: Prx-IV Thioredoxin-dependent peroxiredoxin 4Curated |
Gene namesi | Name:PRDX4 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 40 | By similarityAdd BLAST | 40 | |
ChainiPRO_0000135097 | 41 – 274 | Peroxiredoxin-4Add BLAST | 234 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 127 | Interchain (with C-248); in linked formBy similarity | ||
Disulfide bondi | 248 | Interchain (with C-127); in linked formBy similarity |
Post-translational modificationi
The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.By similarity
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | Q9BGI2 |
PeptideAtlasi | Q9BGI2 |
PRIDEi | Q9BGI2 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000006165, Expressed in spermatocyte and 18 other tissues |
Interactioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.
By similarityProtein-protein interaction databases
BioGRIDi | 159288, 1 interactor |
STRINGi | 9913.ENSBTAP00000008107 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 82 – 240 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 159 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, SignalPhylogenomic databases
eggNOGi | KOG0852, Eukaryota |
HOGENOMi | CLU_042529_21_1_1 |
InParanoidi | Q9BGI2 |
OMAi | EDSESCY |
OrthoDBi | 1326484at2759 |
TreeFami | TF105181 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9BGI2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAPPPPPPL PATTLAPGRS RKLLLLPLLL FLLRAEAVRG FEAEERPRTR
60 70 80 90 100
EEECHFYAGG QVYPGEVSRV SVAEHSLHLS KAKISKPAPY WEGTAVINGE
110 120 130 140 150
FKELKLTDYR GKYLVFFFYP LDFTFVCPTE IIAFGDRIDE FRSINTEVVA
160 170 180 190 200
CSVDSQFTHL AWINTPRRQG GLGSINIPLL ADLNHQISKD YGVYLEDSGH
210 220 230 240 250
TLRGLFIIDD KGILRQITLN DLPVGRSVDE TLRLVQAFQY TDKHGEVCPA
260 270
GWKPGSETII PDPAGKLKYF DKLN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF305563 mRNA Translation: AAG53660.1 BC109824 mRNA Translation: AAI09825.1 |
RefSeqi | NP_776858.1, NM_174433.2 |
Genome annotation databases
GeneIDi | 281999 |
KEGGi | bta:281999 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF305563 mRNA Translation: AAG53660.1 BC109824 mRNA Translation: AAI09825.1 |
RefSeqi | NP_776858.1, NM_174433.2 |
3D structure databases
SMRi | Q9BGI2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 159288, 1 interactor |
STRINGi | 9913.ENSBTAP00000008107 |
Protein family/group databases
PeroxiBasei | 4531, Bt2CysPrx04 |
Proteomic databases
PaxDbi | Q9BGI2 |
PeptideAtlasi | Q9BGI2 |
PRIDEi | Q9BGI2 |
Genome annotation databases
GeneIDi | 281999 |
KEGGi | bta:281999 |
Organism-specific databases
CTDi | 10549 |
Phylogenomic databases
eggNOGi | KOG0852, Eukaryota |
HOGENOMi | CLU_042529_21_1_1 |
InParanoidi | Q9BGI2 |
OMAi | EDSESCY |
OrthoDBi | 1326484at2759 |
TreeFami | TF105181 |
Gene expression databases
Bgeei | ENSBTAG00000006165, Expressed in spermatocyte and 18 other tissues |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR019479, Peroxiredoxin_C IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10417, 1-cysPrx_C, 1 hit PF00578, AhpC-TSA, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRDX4_BOVIN | |
Accessioni | Q9BGI2Primary (citable) accession number: Q9BGI2 Secondary accession number(s): Q32L06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 10, 2021 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families