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Entry version 59 (07 Apr 2021)
Sequence version 1 (01 Jun 2001)
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Protein

Monomethylxanthine methyltransferase 1

Gene

MXMT1

Organism
Coffea arabica (Arabian coffee)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the biosynthesis of caffeine. Catalyzes the conversion of 7-methylxanthine (7mX) to theobromine and of paraxanthine to caffeine. Has a 5-fold preference for 7mX.1 Publication2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=50 µM for 7-methylxanthine1 Publication
  2. KM=148 µM for 7-methylxanthine1 Publication
  3. KM=458 µM for paraxanthine1 Publication
  4. KM=11.9 µM for S-adenosyl-L-methionine1 Publication
  5. KM=12 µM for S-adenosyl-L-methionine1 Publication
  1. Vmax=7.14 pmol/min/µg enzyme toward 7-methylxanthine1 Publication
  2. Vmax=7.94 pmol/min/µg enzyme toward S-adenosyl-L-methionine1 Publication

pH dependencei

Optimum pH is 7.5.3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: Alkaloid biosynthesis

This protein is involved in Alkaloid biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei18S-adenosyl-L-methionineBy similarity1
Binding sitei18SubstrateBy similarity1
Binding sitei60S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei61S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei66S-adenosyl-L-methionineBy similarity1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei154Involved in substrate discriminationBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi178Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi260Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi262Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi263MagnesiumBy similarity1
Sitei266Involved in substrate discriminationBy similarity1
Binding sitei327SubstrateBy similarity1
Sitei337Involved in substrate discriminationBy similarity1
Binding sitei362SubstrateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAlkaloid metabolism
LigandMagnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.1.1.159, 1559
2.1.1.160, 1559

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
Q9AVJ9

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Monomethylxanthine methyltransferase 11 Publication (EC:2.1.1.1592 Publications)
Short name:
CaMXMT11 Publication
Alternative name(s):
7-methylxanthine N-methyltransferase 1Curated
Theobromine synthase 11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MXMT11 Publication
Synonyms:CTS11 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCoffea arabica (Arabian coffee)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri13443 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeIxoroideaeGardenieae complexBertiereae - Coffeeae cladeCoffeeaeCoffea
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000515148 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome assembly

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei

Tobacco plants (Nicotiana tabacum cv. Xanthi) expressing CaXMT1, CaMXMT1 and CaDXMT1 accumulate caffeine and become less appetant and toxic for caterpillars cutworms (Spodoptera litura). Caffeine also stimulates endogenous defense mechanisms against other pathogens (e.g. tobacco mosaic virus and Pseudomonas syringae) by triggering the expression of defense-related genes.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004083031 – 378Monomethylxanthine methyltransferase 1Add BLAST378

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed, at low levels, in stems, young leaves, floral buds and immature fruits (grains), but not in roots, old leaves and mature fruits.3 Publications

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9AVJ9

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni21 – 25Substrate bindingBy similarity5
Regioni60 – 61S-adenosyl-L-methionine bindingBy similarity2
Regioni98 – 101S-adenosyl-L-methionine bindingBy similarity4
Regioni139 – 141S-adenosyl-L-methionine bindingBy similarity3
Regioni156 – 158S-adenosyl-L-methionine bindingBy similarity3
Regioni157 – 161Substrate bindingBy similarity5

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.1200.270, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR005299, MeTrfase_7
IPR042086, MeTrfase_capping
IPR029063, SAM-dependent_MTases

The PANTHER Classification System

More...
PANTHERi
PTHR31009, PTHR31009, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03492, Methyltransf_7, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53335, SSF53335, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9AVJ9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELQEVLHMN EGEGDTSYAK NASYNLALAK VKPFLEQCIR ELLRANLPNI
60 70 80 90 100
NKCIKVADLG CASGPNTLLT VRDIVQSIDK VGQEEKNELE RPTIQIFLND
110 120 130 140 150
LFQNDFNSVF KLLPSFYRKL EKENGRKIGS CLISAMPGSF YGRLFPEESM
160 170 180 190 200
HFLHSCYSVH WLSQVPSGLV IELGIGANKG SIYSSKGCRP PVQKAYLDQF
210 220 230 240 250
TKDFTTFLRI HSKELFSRGR MLLTCICKVD EFDEPNPLDL LDMAINDLIV
260 270 280 290 300
EGLLEEEKLD SFNIPFFTPS AEEVKCIVEE EGSCEILYLE TFKAHYDAAF
310 320 330 340 350
SIDDDYPVRS HEQIKAEYVA SLIRSVYEPI LASHFGEAIM PDLFHRLAKH
360 370
AAKVLHMGKG CYNNLIISLA KKPEKSDV
Length:378
Mass (Da):42,747
Last modified:June 1, 2001 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF36184F3D5243809
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti11E → G in AAM18503 (Ref. 2) Curated1
Sequence conflicti22A → S in AAM18503 (Ref. 2) Curated1
Sequence conflicti34F → V in AAM18503 (Ref. 2) Curated1
Sequence conflicti52K → N in AAM18503 (Ref. 2) Curated1
Sequence conflicti153L → I in AAM18503 (Ref. 2) Curated1
Sequence conflicti159V → F in AAM18503 (Ref. 2) Curated1
Sequence conflicti176G → S in AAM18503 (Ref. 2) Curated1
Sequence conflicti231E → V in BAC43756 (PubMed:12527364).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB048794 mRNA Translation: BAB39216.1
AF494413 mRNA Translation: AAM18503.1
AB034700 mRNA Translation: BAC43756.1
AF494411 mRNA Translation: AAM18501.1
JX978511 Genomic DNA Translation: AFV60439.1
JX978519 mRNA Translation: AFV60447.1

Genome annotation databases

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ag:BAB39216

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048794 mRNA Translation: BAB39216.1
AF494413 mRNA Translation: AAM18503.1
AB034700 mRNA Translation: BAC43756.1
AF494411 mRNA Translation: AAM18501.1
JX978511 Genomic DNA Translation: AFV60439.1
JX978519 mRNA Translation: AFV60447.1

3D structure databases

SMRiQ9AVJ9
ModBaseiSearch...

Genome annotation databases

KEGGiag:BAB39216

Enzyme and pathway databases

BRENDAi2.1.1.159, 1559
2.1.1.160, 1559
SABIO-RKiQ9AVJ9

Family and domain databases

Gene3Di1.10.1200.270, 1 hit
InterProiView protein in InterPro
IPR005299, MeTrfase_7
IPR042086, MeTrfase_capping
IPR029063, SAM-dependent_MTases
PANTHERiPTHR31009, PTHR31009, 1 hit
PfamiView protein in Pfam
PF03492, Methyltransf_7, 1 hit
SUPFAMiSSF53335, SSF53335, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMXMT1_COFAR
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9AVJ9
Secondary accession number(s): A0A096VHZ7, Q8H0G5, Q8RVM1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 1, 2001
Last modified: April 7, 2021
This is version 59 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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