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Protein

Biotin synthase

Gene

bioB

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi72Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi75Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi112Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi143Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi203Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi276Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBDIA224911:G1G3J-2115-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:blr2095
ORF Names:id897
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003812441 – 331Biotin synthaseAdd BLAST331

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr2095

Structurei

3D structure databases

ProteinModelPortaliQ9AMS7
SMRiQ9AMS7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
InParanoidiQ9AMS7
KOiK01012
OMAiKYTWLKP
OrthoDBiPOG091H01DF
PhylomeDBiQ9AMS7

Family and domain databases

Gene3Di3.20.20.701 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9AMS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAAVQVQRK KASNGAQVRN HWNVEEAKAL YDLPFADLML QAQRAHRKNF
60 70 80 90 100
DPNHVETASL LSIKTGGCPE DCGYCSQSAH YATGLKATRL MRCADVVATA
110 120 130 140 150
QRAKDAGATR FCMAAAWRTP KDRDLDSVCD MVNAVKGLGM ETCVTLGTLT
160 170 180 190 200
PKHAARLAEA GLDFYNHNVD TSPEFYSKII TTRSLQDRID TLAHVRDAGI
210 220 230 240 250
KICCGGIIGM GERVEDRLGM LVLLANLPNY PESVPINLWN KIEGVPVEDT
260 270 280 290 300
AEPPDPIALV RLLATARVMM PRSVVRLSAG RQYMTDELQA LCFLAGANSI
310 320 330
FVGDVLLTTN NPKVDRDADL LARLGITSGL A
Length:331
Mass (Da):36,011
Last modified:June 1, 2001 - v1
Checksum:iBE11557840FC5B3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322013 Genomic DNA Translation: AAG61070.1
BA000040 Genomic DNA Translation: BAC47360.1
RefSeqiNP_768735.1, NC_004463.1
WP_011084890.1, NZ_CP011360.1

Genome annotation databases

EnsemblBacteriaiBAC47360; BAC47360; BAC47360
GeneIDi1055459
29270610
KEGGibja:blr2095
PATRICifig|224911.44.peg.1604

Similar proteinsi

Entry informationi

Entry nameiBIOB_BRADU
AccessioniPrimary (citable) accession number: Q9AMS7
Secondary accession number(s): Q79UC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome