UniProtKB - Q9ALJ4 (AGAA_GEOSE)
Protein
Alpha-galactosidase AgaA
Gene
agaA
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Functioni
Hydrolyzes the short-chain alpha-galactosaccharides raffinose and stachyose.1 Publication
Catalytic activityi
- Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.3 Publications EC:3.2.1.22
Activity regulationi
Not inhibited by D-galactose or sucrose (Ref. 1). Inhibited by pharmaceutical drug 1-deoxygalactonojirimycin (PubMed:23012371).2 Publications
Kineticsi
kcat is 105 s(-1) for p-nitrophenyl-alpha-galactopyranoside (at 25 degrees Celsius and pH 6.5). kcat is 180 s(-1) for raffinose (at 25 degrees Celsius and pH 6.5).1 Publication
- KM=0.21 mM for p-nitrophenyl-alpha-galactopyranoside (at 25 degrees Celsius and pH 6.5)1 Publication
- KM=3.8 mM for raffinose (at 25 degrees Celsius and pH 6.5)1 Publication
pH dependencei
Optimum pH is 6.5.1 Publication
Temperature dependencei
Optimum temperature is 65 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 53 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 199 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 443 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 478 | Nucleophile1 Publication | 1 | |
Binding sitei | 526 | SubstrateCombined sources1 Publication | 1 | |
Active sitei | 548 | Proton donor1 Publication | 1 | |
Binding sitei | 548 | SubstrateCombined sources1 Publication | 1 |
GO - Molecular functioni
- alpha-galactosidase activity Source: UniProtKB
- raffinose alpha-galactosidase activity Source: UniProtKB-EC
GO - Biological processi
- protein homotetramerization Source: UniProtKB
- raffinose catabolic process Source: UniProtKB
- stachyose metabolic process Source: UniProtKB
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Protein family/group databases
CAZyi | GH36, Glycoside Hydrolase Family 36 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:agaA1 PublicationImported |
Organismi | Geobacillus stearothermophilus (Bacillus stearothermophilus)Imported |
Taxonomic identifieri | 1422 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus |
Pathology & Biotechi
Biotechnological usei
Has properties that make it suitable for elimination of D-raffinose from low green syrup in sugar manufacturing process from beets in order to improve the yield of sucrose.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 336 | W → A: Very strongly reduced hydrolytic efficiency against raffinose, but displays medium level of transglycosylation activity compared to none with wild-type enzyme. 1 Publication | 1 | |
Mutagenesisi | 336 | W → F or S: Strongly reduced hydrolytic efficiency against raffinose, but displays high level of transglycosylation activity compared to none with wild-type enzyme. 1 Publication | 1 | |
Mutagenesisi | 336 | W → N: Very strongly reduced hydrolytic efficiency against raffinose, but displays low level of transglycosylation activity compared to none with wild-type enzyme. 1 Publication | 1 | |
Mutagenesisi | 478 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 548 | D → N: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000439585 | 1 – 729 | Alpha-galactosidase AgaAAdd BLAST | 729 |
Interactioni
Subunit structurei
Homotetramer.
By similarityStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9ALJ4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 366 – 367 | Substrate bindingCombined sources1 Publication | 2 | |
Regioni | 476 – 480 | Substrate bindingCombined sources1 Publication | 5 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 36 family.Curated
Family and domain databases
CDDi | cd14791, GH36, 1 hit |
Gene3Di | 2.60.40.1180, 1 hit 2.70.98.60, 1 hit 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR038417, Alpga-gal_N_sf IPR000111, Glyco_hydro_27/36_CS IPR002252, Glyco_hydro_36 IPR031705, Glyco_hydro_36_C IPR031704, Glyco_hydro_36_N IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF16874, Glyco_hydro_36C, 1 hit PF16875, Glyco_hydro_36N, 1 hit |
PIRSFi | PIRSF005536, Agal, 1 hit |
PRINTSi | PR00743, GLHYDRLASE36 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00512, ALPHA_GALACTOSIDASE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9ALJ4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVAYNPQTK QFHLRAGKAS YVMQLFRSGY LAHVYWGKAV RDVRGARAFP
60 70 80 90 100
RLDRAFSPNP DPSDRTFSLD TLLQEYPAYG NTDFRAPAYQ VQLENGSTVT
110 120 130 140 150
DLRYKTHRIY KGKPRLNGLP ATYVEHEQEA ETLEIVLGDA LIGLEVTLQY
160 170 180 190 200
TAYEKWNVIT RSARFENKGG ERLKLLRALS MSVDFPTADY DWIHLPGAWG
210 220 230 240 250
RERWIERRPL VTGVQAAESR RGASSHQQNP FIALVAKNAD EHQGEVYGFS
260 270 280 290 300
FVYSGNFLAQ IEVDQFGTAR VSMGINPFDF TWLLQPGESF QTPEVVMVYS
310 320 330 340 350
DQGLNGMSQT YHELYRTRLA RGAFRDRERP ILINNWEATY FDFNEEKIVN
360 370 380 390 400
IARTAAELGI ELVVLDDGWF GERDDDRRSL GDWIVNRRKL PNGLDGLAKQ
410 420 430 440 450
VNELGLQFGL WVEPEMVSPN SELYRKHPDW CLHVPNRPRS EGRNQLVLDY
460 470 480 490 500
SREDVCDYII ETISNVLASA PITYVKWDMN RHMTEIGSSA LPPERQRETA
510 520 530 540 550
HRYMLGLYRV MDEITSRFPH ILFESCSGGG GRFDPGMLYY MPQTWTSDNT
560 570 580 590 600
DAVSRLKIQY GTSLVYPISA MGAHVSAVPN HQVGRVASLK TRGHVAMSGN
610 620 630 640 650
FGYELDITKL TETEKQMMKQ QVAFYKDVRR LVQFGTFYRL LSPFEGNEAA
660 670 680 690 700
WMFVSADRSE ALVAYFRVLA EANAPLSYLR LKGLDSNQDY EIEGLGVYGG
710 720
DELMYAGVAL PYRSSDFISM MWRLKAVQQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY013286 Genomic DNA Translation: AAG49420.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY013286 Genomic DNA Translation: AAG49420.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4FNP | X-ray | 2.80 | A/B/C/D | 1-729 | [»] | |
4FNR | X-ray | 3.20 | A/B/C/D | 1-729 | [»] | |
4FNS | X-ray | 2.60 | A/B/C/D | 1-729 | [»] | |
4FNT | X-ray | 2.60 | A/B/C/D | 1-729 | [»] | |
4FNU | X-ray | 3.60 | A/B/C/D | 1-729 | [»] | |
SMRi | Q9ALJ4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
CAZyi | GH36, Glycoside Hydrolase Family 36 |
Family and domain databases
CDDi | cd14791, GH36, 1 hit |
Gene3Di | 2.60.40.1180, 1 hit 2.70.98.60, 1 hit 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR038417, Alpga-gal_N_sf IPR000111, Glyco_hydro_27/36_CS IPR002252, Glyco_hydro_36 IPR031705, Glyco_hydro_36_C IPR031704, Glyco_hydro_36_N IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF16874, Glyco_hydro_36C, 1 hit PF16875, Glyco_hydro_36N, 1 hit |
PIRSFi | PIRSF005536, Agal, 1 hit |
PRINTSi | PR00743, GLHYDRLASE36 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00512, ALPHA_GALACTOSIDASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AGAA_GEOSE | |
Accessioni | Q9ALJ4Primary (citable) accession number: Q9ALJ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2017 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 26, 2020 | |
This is version 63 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries