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UniProtKB - Q9AJS8 (3HBCL_THAAR)
Protein
3-hydroxybenzoate--CoA/4-hydroxybenzoate--CoA ligase
Gene
hcl
Organism
Thauera aromatica
Status
Functioni
Catalyzes the ligation of 3-hydroxybenzoate or 4-hydroxybenzoate and CoA at the expense of ATP. The enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates.
1 PublicationCatalytic activityi
Kineticsi
- KM=60 µM for 3-hydroxybenzoate1 Publication
- KM=65 µM for 4-hydroxybenzoate1 Publication
- Vmax=4.1 µmol/min/mg enzyme with 3-hydroxybenzoate as substrate1 Publication
- Vmax=3.4 µmol/min/mg enzyme with 4-hydroxybenzoate as substrate1 Publication
pH dependencei
Optimum pH is above 9.1 Publication
GO - Molecular functioni
- 4-hydroxybenzoate-CoA ligase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.2.1.27, 6271 6.2.1.37, 6271 |
SABIO-RKi | Q9AJS8 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hcl |
Organismi | Thauera aromatica |
Taxonomic identifieri | 59405 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Zoogloeaceae › Thauera |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000350736 | 2 – 523 | 3-hydroxybenzoate--CoA/4-hydroxybenzoate--CoA ligaseAdd BLAST | 522 |
Expressioni
Inductioni
By 3-hydroxybenzoate.1 Publication
Family & Domainsi
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR011957, Benz_CoA_lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02262, benz_CoA_lig, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9AJS8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEQLQPQQS MNAADEIIGR PLAQGLGEQT AMLCAERSIT YRELDAATNR
60 70 80 90 100
HGNALRAHGV GKGDRVLFLM DDSPELVAAY LGTLRIGAVA VALNVRLAPR
110 120 130 140 150
DVLYVIQDSA CRLLYIDAEF LHLYQQIAGE LEQPPQVVVR GDEAPAPAII
160 170 180 190 200
AFKHFLDGQA ATLESVQVAP DDVAYWLYSS GTTGRPKAVM HAHRSVLIAD
210 220 230 240 250
RLEREYFGIK PGDRVFTTSK MFFGWSLGHS LMGGLQCGAT VIVAPGWPDA
260 270 280 290 300
ERVMATAARH RPTILFSTPV MYRNLLREGA GESAAMRDIR HFVSAGEKLP
310 320 330 340 350
ENIGQQWLDT FGIPITEGIG ASETVFLFLC ARPDAYRIGS CGKRVPWAEV
360 370 380 390 400
RLLDELGNEI TTPDTPGLIA IRMASQFVGY WKLPETTEKA LRDGWYYPGD
410 420 430 440 450
MFSFDADGFW YHNGRADDML KISGQWVSPG EIESCASAVP GIAEAVVVAV
460 470 480 490 500
PNDDGLTRLT LFIVPEDPSA SQQKLSEAWM TTLRGTLSIY KCPRTIQFLE
510 520
ELPRTATGKV QKYRLRDMLQ ATL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278289 Genomic DNA Translation: CAC28158.1 |
Genome annotation databases
KEGGi | ag:CAC28158 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278289 Genomic DNA Translation: CAC28158.1 |
3D structure databases
SMRi | Q9AJS8 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:CAC28158 |
Enzyme and pathway databases
BRENDAi | 6.2.1.27, 6271 6.2.1.37, 6271 |
SABIO-RKi | Q9AJS8 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR011957, Benz_CoA_lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
TIGRFAMsi | TIGR02262, benz_CoA_lig, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 3HBCL_THAAR | |
Accessioni | Q9AJS8Primary (citable) accession number: Q9AJS8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 23, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 59 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families