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UniProtKB - Q9AIX6 (BOXA_AROEV)
Protein
Benzoyl-CoA oxygenase component A
Gene
boxA
Organism
Aromatoleum evansii (Azoarcus evansii)
Status
Functioni
The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-epoxy-2,3-dihydrobenzoyl-CoA. BoxA acts as a reductase that uses NADPH to reduce the oxygenase component BoxB. BoxAB does not act on NADH or benzoate.
1 PublicationCatalytic activityi
- EC:1.14.13.2081 Publication
Cofactori
Protein has several cofactor binding sites:- FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication
- [4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters per subunit.1 Publication
Kineticsi
- KM=0.03 mM for benzoyl-CoA1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 274 – 291 | NADPBy similarityAdd BLAST | 18 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- dioxygenase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- aromatic compound catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | 4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NADP |
Enzyme and pathway databases
BRENDAi | 1.14.13.208, 603 |
SABIO-RKi | Q9AIX6 |
Names & Taxonomyi
Protein namesi | Recommended name: Benzoyl-CoA oxygenase component A (EC:1.14.13.2081 Publication)Alternative name(s): Benzoyl-CoA 2,3-dioxygenase subunit A Benzoyl-CoA dioxygenase reductase component |
Gene namesi | Name:boxA |
Organismi | Aromatoleum evansii (Azoarcus evansii) |
Taxonomic identifieri | 59406 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Aromatoleum |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000350726 | 1 – 414 | Benzoyl-CoA oxygenase component AAdd BLAST | 414 |
Expressioni
Inductioni
By benzoate.1 Publication
Interactioni
Subunit structurei
Homodimer. The subunit composition of the active BoxA/BoxB protein complex is not known.
1 PublicationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 41 | 4Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST | 30 | |
Domaini | 42 – 70 | 4Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST | 29 | |
Domaini | 143 – 265 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 123 |
Keywords - Domaini
RepeatFamily and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR017634, Benzoyl_CoA_Oase_BoxA IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR015701, FNR IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR43314, PTHR43314, 1 hit |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00037, Fer4, 2 hits PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF501177, BoxA, 1 hit PIRSF000361, Frd-NADP+_RD, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
TIGRFAMsi | TIGR03224, benzo_boxA, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9AIX6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNAPAEHANL ARQHLIDPEI CIRCNTCEEI CPVDAITHDS RNYVVKFETC
60 70 80 90 100
NGCLACISPC PTGAIDSWRN VDKATPHSLA DQYSWDYLPD TTELDQFEAT
110 120 130 140 150
VMGAAELPAE VQQITEVATA GQGGPAMAPW SASHPYVNLY TPANPITATV
160 170 180 190 200
TGNYRLTAED ASSDIHHIVL DFGTTPFPVL EGQSIGIIPP GVDEKGKPHL
210 220 230 240 250
LRMYSVASPR DGERPHYNNL SLTVKRVVED HEGNPTRGVA SNYVCDLKKG
260 270 280 290 300
DKVQVTGPYG STYLMPNHPG SSIMMICTGT GSAPMRAMTE RRRRRMDRKE
310 320 330 340 350
GGELVLFFGA RAPEELPYFG PLQKLPKEFI DINFAFSRVP GEPKRYVQDA
360 370 380 390 400
IRERADKVFQ MLQDDNCYIY ICGLKGMEAG VLEAFRDICR AKGADWDALR
410
PQLLSKARFH VETY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF220510 Genomic DNA Translation: AAK00600.1 AF548005 Genomic DNA Translation: AAN39377.1 |
Genome annotation databases
KEGGi | ag:AAN39377 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF220510 Genomic DNA Translation: AAK00600.1 AF548005 Genomic DNA Translation: AAN39377.1 |
3D structure databases
AlphaFoldDBi | Q9AIX6 |
SMRi | Q9AIX6 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:AAN39377 |
Enzyme and pathway databases
BRENDAi | 1.14.13.208, 603 |
SABIO-RKi | Q9AIX6 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR017634, Benzoyl_CoA_Oase_BoxA IPR008333, Cbr1-like_FAD-bd_dom IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR015701, FNR IPR039261, FNR_nucleotide-bd IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
PANTHERi | PTHR43314, PTHR43314, 1 hit |
Pfami | View protein in Pfam PF00970, FAD_binding_6, 1 hit PF00037, Fer4, 2 hits PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF501177, BoxA, 1 hit PIRSF000361, Frd-NADP+_RD, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
TIGRFAMsi | TIGR03224, benzo_boxA, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BOXA_AROEV | |
Accessioni | Q9AIX6Primary (citable) accession number: Q9AIX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 23, 2008 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |