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Entry version 72 (11 Dec 2019)
Sequence version 1 (01 Jun 2001)
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Protein

1-butanol dehydrogenase (quinone)

Gene

boh

Organism
Thauera butanivorans (strain ATCC 43655 / DSM 2080/ JCM 20651 / NBRC 103042 / IAM 12574 / Bu B1211) (Pseudomonas butanovora)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the metabolism of butane (PubMed:11889098). May function primarily in energy generation (PubMed:12142403). Catalyzes the oxidation of 1-butanol to 1-butanal (PubMed:11889098, PubMed:12142403). Also able to use 2-butanol and butyraldehyde, although the affinity is comparatively low (PubMed:12142403).2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=1.7 µM for 1-butanol1 Publication
  2. KM=369 µM for butyraldehyde1 Publication
  3. KM=662 µM for 2-butanol1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi40Calcium 1By similarity1
    Metal bindingi43Calcium 1By similarity1
    Metal bindingi46Calcium 1By similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei90Pyrroloquinoline quinoneBy similarity1
    Binding sitei140Pyrroloquinoline quinoneBy similarity1
    Binding sitei184Pyrroloquinoline quinoneBy similarity1
    Metal bindingi208Calcium 2By similarity1
    Metal bindingi295Calcium 2By similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei345Proton acceptorBy similarity1
    Metal bindingi345Calcium 2By similarity1
    Binding sitei374Pyrroloquinoline quinoneBy similarity1
    Binding sitei592Pyrroloquinoline quinone; via amide nitrogenBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    LigandCalcium, Metal-binding, PQQ

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:MONOMER-19862

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    1.2.99.3 8965

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    1-butanol dehydrogenase (quinone)1 Publication (EC:1.1.5.111 Publication1 Publication)
    Alternative name(s):
    PQQ-containing alcohol dehydrogenase1 Publication
    Quinoprotein alcohol dehydrogenase1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:boh1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiThauera butanivorans (strain ATCC 43655 / DSM 2080/ JCM 20651 / NBRC 103042 / IAM 12574 / Bu B1211) (Pseudomonas butanovora)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1219356 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesZoogloeaceaeThauera

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Periplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Cells lacking this gene show a delayed growth on butane and are unable to tolerate high level of 1-butanol (PubMed:11889098, PubMed:12142403). When both bdh and boh genes are inactivated, growth on butane and 1-butanol is eliminated (PubMed:11889098).2 Publications

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 28Sequence analysisAdd BLAST28
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_500432398629 – 6231-butanol dehydrogenase (quinone)Add BLAST595

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi134 ↔ 135By similarity

    Keywords - PTMi

    Disulfide bond

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    By butane, 1-butanol and 2-butanol.2 Publications

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q9AGW3

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni202 – 204Pyrroloquinoline quinone bindingBy similarity3

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the bacterial PQQ dehydrogenase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    KEGG Orthology (KO)

    More...
    KOi
    K20937

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd10277 PQQ_ADH_I, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR034119 ADHI
    IPR018391 PQQ_beta_propeller_repeat
    IPR017512 PQQ_MeOH/EtOH_DH
    IPR002372 PQQ_repeat
    IPR011047 Quinoprotein_ADH-like_supfam
    IPR001479 Quinoprotein_DH_CS

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF01011 PQQ, 1 hit
    PF13360 PQQ_2, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00564 PQQ, 4 hits

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF50998 SSF50998, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR03075 PQQ_enz_alc_DH, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00364 BACTERIAL_PQQ_2, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9AGW3-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKKSHAKPFA LRAIVVATAA ALSLPAAAVT DVTWEDIAND HKTTGDVLTY
    60 70 80 90 100
    GLGLKAQRHS PLKAINTDNV ANLVPAWSFS FGGEKQRGQE AQVLVHDGVI
    110 120 130 140 150
    YATASYSRIF AIDARSGKRL WEYNARLPDD IRPCCDVVNR GAAIYGDKVF
    160 170 180 190 200
    FGTLDAAMVA LDRKTGKVVW RKKFGDHKVG YTMTGAPFVI KDQKSGRTLL
    210 220 230 240 250
    VHGSSGDEFG VVGWLFARDP DTGEEVWARP MVEGHMGRLN GKDSTPTGDP
    260 270 280 290 300
    KAPSWPDDPN SPTGKVEAWS QGGGAPWQTA SFDVENNMVV IGAGNPAPWN
    310 320 330 340 350
    TWKRTAPGDD PRNWDSLFTS GQAYVDASTG ELKGFYQHTP NDAWDFSGNN
    360 370 380 390 400
    SVVLFEYKDP KTGKMVNASA HADRNGFFFV TDRDMLAKGA GYPNKPTSLI
    410 420 430 440 450
    GAWPFVDGIT WASGFDLKTG KPIEKDNRPP QPKEGADKGE SIFVSPPFLG
    460 470 480 490 500
    GTNWHPMSYS PDTGLFYIPA NHWAMDYWTE NVTYKAGSAY LGQGFRIKNL
    510 520 530 540 550
    FDDHVGILRA IDPSPARSLG AQGRVPAVAG TLTTAGGWVF TGTSDGYLKA
    560 570 580 590 600
    FDAKNGKELW KFQTGSGVVS VPVTWEMDGE QYVAIQSGYG GAVPLWGGDM
    610 620
    AELTKQVTQG GSMWVFKLPK ASR
    Length:623
    Mass (Da):67,553
    Last modified:June 1, 2001 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA573A1C64AC12D55
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AF326086 Genomic DNA Translation: AAK15506.1

    Genome annotation databases

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ag:AAK15506

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF326086 Genomic DNA Translation: AAK15506.1

    3D structure databases

    SMRiQ9AGW3
    ModBaseiSearch...

    Genome annotation databases

    KEGGiag:AAK15506

    Phylogenomic databases

    KOiK20937

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-19862
    BRENDAi1.2.99.3 8965

    Family and domain databases

    CDDicd10277 PQQ_ADH_I, 1 hit
    InterProiView protein in InterPro
    IPR034119 ADHI
    IPR018391 PQQ_beta_propeller_repeat
    IPR017512 PQQ_MeOH/EtOH_DH
    IPR002372 PQQ_repeat
    IPR011047 Quinoprotein_ADH-like_supfam
    IPR001479 Quinoprotein_DH_CS
    PfamiView protein in Pfam
    PF01011 PQQ, 1 hit
    PF13360 PQQ_2, 1 hit
    SMARTiView protein in SMART
    SM00564 PQQ, 4 hits
    SUPFAMiSSF50998 SSF50998, 1 hit
    TIGRFAMsiTIGR03075 PQQ_enz_alc_DH, 1 hit
    PROSITEiView protein in PROSITE
    PS00364 BACTERIAL_PQQ_2, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBOH_THABB
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9AGW3
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2018
    Last sequence update: June 1, 2001
    Last modified: December 11, 2019
    This is version 72 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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